##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211414_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12120587 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.8323046565319 31.0 31.0 34.0 27.0 34.0 2 30.88221997828983 31.0 31.0 34.0 27.0 34.0 3 30.937974786204663 31.0 31.0 34.0 27.0 34.0 4 34.539986800969295 37.0 35.0 37.0 30.0 37.0 5 34.587847271753425 37.0 35.0 37.0 30.0 37.0 6 34.65058969503705 37.0 35.0 37.0 30.0 37.0 7 34.55939551442517 37.0 35.0 37.0 30.0 37.0 8 34.55761639267141 37.0 35.0 37.0 30.0 37.0 9 36.05292664455938 39.0 35.0 39.0 30.0 39.0 10-11 35.96464490539938 39.0 35.0 39.0 30.0 39.0 12-13 35.862979367253416 39.0 35.0 39.0 30.0 39.0 14-15 36.95895598950777 40.0 36.0 41.0 30.0 41.0 16-17 36.952652045647625 40.0 36.0 41.0 30.0 41.0 18-19 36.94746582818142 40.0 36.0 41.0 30.0 41.0 20-21 36.930500271975276 40.0 36.0 41.0 30.0 41.0 22-23 36.79955298369625 39.5 36.0 41.0 30.0 41.0 24-25 36.696428687818496 39.0 36.0 41.0 29.0 41.0 26-27 36.546976685205095 39.0 36.0 41.0 29.0 41.0 28-29 36.42102024431655 39.0 35.0 41.0 29.0 41.0 30-31 36.26879931640275 39.0 35.0 40.5 28.0 41.0 32-33 36.0875256289155 39.0 35.0 40.0 27.5 41.0 34-35 35.95600786496561 38.5 35.0 40.0 27.0 41.0 36-37 35.82097001572613 38.0 35.0 40.0 27.0 41.0 38-39 35.664215272742155 38.0 34.0 40.0 26.5 41.0 40-41 35.500386821199335 38.0 34.0 40.0 26.0 41.0 42-43 35.3019840128205 38.0 34.0 40.0 25.0 41.0 44-45 35.1615354520371 38.0 33.5 40.0 25.0 41.0 46-47 34.93991871020768 38.0 33.0 40.0 24.0 41.0 48-49 34.696968471906516 38.0 33.0 40.0 24.0 41.0 50-51 34.0734422763518 37.5 32.0 39.5 23.5 40.5 52-53 34.40598945414112 37.5 33.0 39.5 24.0 40.5 54-55 34.81793307535352 38.0 33.0 40.0 24.0 41.0 56-57 34.738616619805626 38.0 33.0 40.0 24.0 41.0 58-59 34.52926685811504 38.0 33.0 40.0 23.0 41.0 60-61 34.312833157338005 37.0 33.0 40.0 23.0 41.0 62-63 34.045012588911746 37.0 32.0 40.0 23.0 41.0 64-65 33.84020361390088 36.0 32.0 40.0 23.0 41.0 66-67 33.64617926508015 36.0 32.0 39.0 22.5 41.0 68-69 33.31660574689988 35.5 32.0 39.0 22.0 41.0 70-71 32.95943987696305 35.0 31.0 39.0 21.5 40.5 72-73 32.60281997893337 35.0 31.0 38.0 20.5 40.0 74-75 32.24881855969517 35.0 31.0 37.0 20.0 39.5 76-77 31.892790258425602 35.0 30.0 37.0 20.0 39.0 78-79 31.526890364303313 35.0 30.0 36.0 19.5 39.0 80-81 31.16438902670308 34.0 30.0 36.0 18.5 38.0 82-83 30.84219572038879 34.0 29.0 35.0 18.0 37.0 84-85 30.54712634792358 34.0 29.0 35.0 18.0 37.0 86-87 30.255533704761987 34.0 29.0 35.0 17.0 36.0 88-89 29.98256371576723 34.0 29.0 35.0 16.0 36.0 90-91 29.719412599406283 34.0 28.5 35.0 15.0 36.0 92-93 29.47157427276418 34.0 27.5 35.0 12.5 35.0 94-95 29.24678474730638 34.0 27.0 35.0 11.0 35.0 96-97 29.006317392053703 34.0 26.5 35.0 10.0 35.0 98-99 28.73520288250066 33.0 25.5 35.0 10.0 35.0 100 27.506228287458356 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35419654488375585 1101 2 -0.3390247032268867 1101 3 -0.40726141531068905 1101 4 -0.2938193458085152 1101 5 -0.2673975484479101 1101 6 -0.2684497472171685 1101 7 -0.2797480810070496 1101 8 -0.2531540316549794 1101 9 -0.37111954115064094 1101 10-11 -0.4019050208498811 1101 12-13 -0.4334098097937584 1101 14-15 -0.6698608674993096 1101 16-17 -0.6262357168619417 1101 18-19 -0.6054458868532393 1101 20-21 -0.6037177768392041 1101 22-23 -0.5564786210503527 1101 24-25 -0.6475817690169023 1101 26-27 -0.762779763290844 1101 28-29 -0.7327023478641408 1101 30-31 -0.7761878222864453 1101 32-33 -0.7212435041496406 1101 34-35 -0.7735831422868742 1101 36-37 -0.7696889356378875 1101 38-39 -0.8049046844984034 1101 40-41 -0.9573118699413996 1101 42-43 -0.9158881788100715 1101 44-45 -0.9860363206581155 1101 46-47 -1.0838368801466132 1101 48-49 -1.167692636161071 1101 50-51 -1.029572497489049 1101 52-53 -0.82779339945494 1101 54-55 -0.8042049080278915 1101 56-57 -0.8729497482623216 1101 58-59 -0.7942483260151434 1101 60-61 -0.8168886628015244 1101 62-63 -0.9467022277940274 1101 64-65 -0.8275332867137237 1101 66-67 -0.8771285340826722 1101 68-69 -0.9995011991102345 1101 70-71 -0.9540471476856425 1101 72-73 -0.9970725404864069 1101 74-75 -1.1113265861027557 1101 76-77 -1.1177102259269027 1101 78-79 -1.071343495357901 1101 80-81 -1.20847581746197 1101 82-83 -1.2237666379015089 1101 84-85 -1.1898474663667216 1101 86-87 -1.2829502971776563 1101 88-89 -1.4132420016111773 1101 90-91 -1.5161276880558852 1101 92-93 -1.6351272238634245 1101 94-95 -1.7104177392181263 1101 96-97 -1.839649556023378 1101 98-99 -1.913216163443959 1101 100 -1.8779710759344646 1102 1 -0.3807099126985456 1102 2 -0.4000095277582325 1102 3 -0.45069358993177033 1102 4 -0.4007564718079948 1102 5 -0.3164635245050107 1102 6 -0.35315594157512464 1102 7 -0.41434890446385 1102 8 -0.3983707711915443 1102 9 -0.60761052101914 1102 10-11 -0.48826636436196225 1102 12-13 -0.6462782464170473 1102 14-15 -0.8196172570348637 1102 16-17 -0.7669675384819215 1102 18-19 -0.7410263974278379 1102 20-21 -0.8538747914921032 1102 22-23 -0.8188356693317544 1102 24-25 -0.8379002200278904 1102 26-27 -0.9556116007446391 1102 28-29 -0.8904421947319605 1102 30-31 -0.8698782156662332 1102 32-33 -0.9163259748337822 1102 34-35 -0.979426778634469 1102 36-37 -0.9525937753461733 1102 38-39 -1.098736479303831 1102 40-41 -1.2300086738132876 1102 42-43 -1.2400331401495635 1102 44-45 -1.2645634026219312 1102 46-47 -1.3507184063898237 1102 48-49 -1.3574377116217278 1102 50-51 -1.2075902745086324 1102 52-53 -0.9891170089585657 1102 54-55 -1.0466614152631664 1102 56-57 -1.0555459264776346 1102 58-59 -1.0144872598645733 1102 60-61 -1.0837909985109064 1102 62-63 -1.015273021892078 1102 64-65 -1.2067070798259536 1102 66-67 -1.1271473336828564 1102 68-69 -1.0920263359159321 1102 70-71 -1.1551422550345478 1102 72-73 -1.0647772294057205 1102 74-75 -1.1880233664445043 1102 76-77 -1.2425927835745156 1102 78-79 -1.3348477849511582 1102 80-81 -1.3690907531192984 1102 82-83 -1.4452407324446241 1102 84-85 -1.513282479310746 1102 86-87 -1.5160818105528264 1102 88-89 -1.580537070037309 1102 90-91 -1.6119825355340183 1102 92-93 -1.7391290386253502 1102 94-95 -1.7430837725319037 1102 96-97 -1.8681657552981612 1102 98-99 -1.8895557023610934 1102 100 -1.8411776847660128 1103 1 -0.43256916287832325 1103 2 -0.5035742148563216 1103 3 -0.5371909470556417 1103 4 -0.3604817240840035 1103 5 -0.3235321748446438 1103 6 -0.3863596589016467 1103 7 -0.44008096451481293 1103 8 -0.43277495245123987 1103 9 -0.5611775679364754 1103 10-11 -0.6114390850781248 1103 12-13 -0.6445489522523431 1103 14-15 -0.7803821412406009 1103 16-17 -0.8129698346710512 1103 18-19 -0.8594999610973346 1103 20-21 -0.6833213527578295 1103 22-23 -0.7128818251295286 1103 24-25 -0.7994116126118698 1103 26-27 -0.7916272025904405 1103 28-29 -0.83744516357077 1103 30-31 -0.860446426949963 1103 32-33 -0.9321263328301015 1103 34-35 -0.8753284404734174 1103 36-37 -0.9627557682349845 1103 38-39 -0.9918541494441726 1103 40-41 -0.984585375265226 1103 42-43 -1.0370424679524248 1103 44-45 -1.056658056712493 1103 46-47 -1.129575089750098 1103 48-49 -1.0481597199760628 1103 50-51 -1.011843296190392 1103 52-53 -0.8796978774546247 1103 54-55 -0.8549172165934209 1103 56-57 -0.847807063185158 1103 58-59 -0.7415273600342474 1103 60-61 -0.8550922651793087 1103 62-63 -0.8544219091624186 1103 64-65 -0.8140410539939111 1103 66-67 -0.9719570954172099 1103 68-69 -0.9382168509645012 1103 70-71 -0.8911500812152013 1103 72-73 -0.9314456082568512 1103 74-75 -0.9701722567101569 1103 76-77 -1.0470158903417257 1103 78-79 -1.0439688384975199 1103 80-81 -1.0329688825007004 1103 82-83 -1.1172639378466123 1103 84-85 -1.2317481580267398 1103 86-87 -1.2872319799781309 1103 88-89 -1.2910569540038246 1103 90-91 -1.2734842500256782 1103 92-93 -1.408136666224987 1103 94-95 -1.3054618340330855 1103 96-97 -1.4395990220789265 1103 98-99 -1.4473705696433221 1103 100 -1.4298694956913032 1104 1 -0.4830631748688816 1104 2 -0.43054097171373584 1104 3 -0.3844223232434274 1104 4 -0.24282244513074858 1104 5 -0.32394879944313004 1104 6 -0.2954114440052251 1104 7 -0.3114220094318867 1104 8 -0.4180220633437912 1104 9 -0.5015845157616567 1104 10-11 -0.474338208507497 1104 12-13 -0.4953922721574955 1104 14-15 -0.7062391533752219 1104 16-17 -0.7248261110425318 1104 18-19 -0.7885546012309916 1104 20-21 -0.7660387519257554 1104 22-23 -0.7196953875030161 1104 24-25 -0.7084410215311365 1104 26-27 -0.6696582705398626 1104 28-29 -0.940241582309504 1104 30-31 -0.9539362628319097 1104 32-33 -0.897987772313698 1104 34-35 -0.8167336511655705 1104 36-37 -0.9664433919132875 1104 38-39 -0.999117373355773 1104 40-41 -0.935081473799336 1104 42-43 -0.999107595361842 1104 44-45 -0.9543601565563407 1104 46-47 -0.9812301663134164 1104 48-49 -1.0212296258552698 1104 50-51 -0.9825792092706962 1104 52-53 -0.7626470596863228 1104 54-55 -0.7993958888402446 1104 56-57 -0.7584752639364183 1104 58-59 -0.7507352881472329 1104 60-61 -0.8127179006130589 1104 62-63 -0.8808554800291617 1104 64-65 -0.7515685432075898 1104 66-67 -0.7865249149524018 1104 68-69 -0.874229315724854 1104 70-71 -0.8656954470333176 1104 72-73 -0.9191034180782083 1104 74-75 -0.9014350414214469 1104 76-77 -0.9998538774748198 1104 78-79 -0.9886783417513598 1104 80-81 -1.0495713841524044 1104 82-83 -1.0759871179943907 1104 84-85 -1.0872781055991965 1104 86-87 -1.15939364651301 1104 88-89 -1.0514729822841282 1104 90-91 -1.1070951430600537 1104 92-93 -1.1960081016204391 1104 94-95 -1.2911695807707062 1104 96-97 -1.3550567524406034 1104 98-99 -1.3988414716817523 1104 100 -1.290434797349917 1105 1 -0.4812808511859892 1105 2 -0.43933156577808674 1105 3 -0.4351503519450226 1105 4 -0.3210741004749096 1105 5 -0.452396360552747 1105 6 -0.38202338461339025 1105 7 -0.4168784018612186 1105 8 -0.42325492571951173 1105 9 -0.5426440436053213 1105 10-11 -0.5131422650900248 1105 12-13 -0.557768798505073 1105 14-15 -0.7055504671925092 1105 16-17 -0.7128965679613231 1105 18-19 -0.6636736085085957 1105 20-21 -0.7618324834023014 1105 22-23 -0.746085227297506 1105 24-25 -0.7000793915521442 1105 26-27 -0.7205563096380345 1105 28-29 -0.7379094238728783 1105 30-31 -0.8584305693085952 1105 32-33 -0.9572904722708984 1105 34-35 -0.919275469881093 1105 36-37 -0.8771662261729389 1105 38-39 -0.9460383986279695 1105 40-41 -0.9196539427959749 1105 42-43 -0.8954177742396823 1105 44-45 -0.9876647129886464 1105 46-47 -1.0708327530840265 1105 48-49 -1.0773609115334466 1105 50-51 -1.011485202934395 1105 52-53 -0.8785180776970591 1105 54-55 -0.8762891899311498 1105 56-57 -0.8574631930061543 1105 58-59 -0.7258549869925446 1105 60-61 -0.7962397629895435 1105 62-63 -0.8476682980062336 1105 64-65 -0.8372743380071554 1105 66-67 -0.7939976883156632 1105 68-69 -0.8267261056980502 1105 70-71 -0.8443156311286373 1105 72-73 -0.8195480192780025 1105 74-75 -0.8310005985829747 1105 76-77 -0.9253658873165946 1105 78-79 -0.9641693740391197 1105 80-81 -0.9545983898716663 1105 82-83 -1.0128323858654618 1105 84-85 -0.9255067778429762 1105 86-87 -0.9251746454595242 1105 88-89 -0.9663945930836491 1105 90-91 -1.0725350070209991 1105 92-93 -1.0382978131675422 1105 94-95 -1.1976406182990154 1105 96-97 -1.1988969203839837 1105 98-99 -1.1527630448292534 1105 100 -1.1440472013084495 1106 1 -0.45803949291553536 1106 2 -0.4469662271765955 1106 3 -0.5167580669674159 1106 4 -0.3468610869902662 1106 5 -0.40600764299826864 1106 6 -0.394974065745032 1106 7 -0.4621199925505266 1106 8 -0.3675514151633621 1106 9 -0.6542454576471357 1106 10-11 -0.6995563630584698 1106 12-13 -0.5762572026608268 1106 14-15 -0.763453327113389 1106 16-17 -0.7542136952367713 1106 18-19 -0.7587771346051753 1106 20-21 -0.8229441102357242 1106 22-23 -0.7149280129039539 1106 24-25 -0.772895152851099 1106 26-27 -0.7504112564349654 1106 28-29 -0.7960002359246445 1106 30-31 -0.7843786789341962 1106 32-33 -0.8865681424346121 1106 34-35 -0.8689313226531468 1106 36-37 -0.8171480223214331 1106 38-39 -0.943670476830718 1106 40-41 -0.9490310786026157 1106 42-43 -0.8935230333924338 1106 44-45 -0.9514422918122278 1106 46-47 -0.966017052754907 1106 48-49 -0.8366436151469117 1106 50-51 -0.7378745531082416 1106 52-53 -0.6775875083602543 1106 54-55 -0.6825375553779409 1106 56-57 -0.7356157748381094 1106 58-59 -0.6735068345505297 1106 60-61 -0.6445880190344369 1106 62-63 -0.700301129163563 1106 64-65 -0.7812317457621987 1106 66-67 -0.7769178134734531 1106 68-69 -0.7250264568705589 1106 70-71 -0.7159862482282051 1106 72-73 -0.7431948728103492 1106 74-75 -0.722244424527382 1106 76-77 -0.7582446057031049 1106 78-79 -0.8015512077321958 1106 80-81 -0.7341951087775662 1106 82-83 -0.8203739880381882 1106 84-85 -0.8122462622077649 1106 86-87 -0.8191911664518301 1106 88-89 -0.8487988698656217 1106 90-91 -0.863083868500965 1106 92-93 -0.8765009059886211 1106 94-95 -0.8543472316697915 1106 96-97 -0.950617587760739 1106 98-99 -1.0166583458655367 1106 100 -1.1578563881793968 1107 1 -0.5490243524808243 1107 2 -0.5434384773411303 1107 3 -0.5818906145585778 1107 4 -0.4026749491684427 1107 5 -0.3969723504221605 1107 6 -0.4720996172597012 1107 7 -0.5175774304701832 1107 8 -0.5205710333074549 1107 9 -0.598011622699218 1107 10-11 -0.6281089433476907 1107 12-13 -0.6662259059948994 1107 14-15 -0.8457076981113261 1107 16-17 -0.8895961944375372 1107 18-19 -0.8939906214009241 1107 20-21 -0.8420399441918818 1107 22-23 -0.8373668942450436 1107 24-25 -0.8609324651570134 1107 26-27 -0.8726616505803904 1107 28-29 -0.9073455011931841 1107 30-31 -0.9545245098709643 1107 32-33 -0.9809738758374777 1107 34-35 -1.003443808829637 1107 36-37 -0.9472238266716175 1107 38-39 -0.9597916465366225 1107 40-41 -0.9786801290219245 1107 42-43 -0.9557581117573193 1107 44-45 -1.0190660780436929 1107 46-47 -0.8863142734967866 1107 48-49 -1.0112657969372947 1107 50-51 -1.055663130739788 1107 52-53 -0.899146143705913 1107 54-55 -0.8051863503939245 1107 56-57 -0.7919162503842543 1107 58-59 -0.8638729208156164 1107 60-61 -0.8588044860969362 1107 62-63 -0.8139928216059147 1107 64-65 -0.7739299659523127 1107 66-67 -0.8392539548657112 1107 68-69 -0.8237196100178821 1107 70-71 -0.7289519316494619 1107 72-73 -0.7251603518017973 1107 74-75 -0.7003992162404984 1107 76-77 -0.7699895959289726 1107 78-79 -0.8033498954167904 1107 80-81 -0.8130007274058322 1107 82-83 -0.8573122585772808 1107 84-85 -0.8674997410161396 1107 86-87 -0.9222880676372256 1107 88-89 -0.9727644806534563 1107 90-91 -0.99786692377225 1107 92-93 -1.00830063304306 1107 94-95 -0.9774662956185125 1107 96-97 -1.0402668455947968 1107 98-99 -0.9881919766362124 1107 100 -1.0206795677189042 1108 1 -0.4943244264763109 1108 2 -0.5497858058124656 1108 3 -0.6181775823039182 1108 4 -0.48254732755724206 1108 5 -0.41223758933957555 1108 6 -0.4486253747432656 1108 7 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2116 40-41 0.5067283782933032 2116 42-43 0.5126929575767107 2116 44-45 0.446533181487915 2116 46-47 0.41889705465090543 2116 48-49 0.4748920199714348 2116 50-51 0.3389414794517265 2116 52-53 0.1984006092408066 2116 54-55 0.2805855065554468 2116 56-57 0.25020683773799135 2116 58-59 0.18186252303227235 2116 60-61 0.3882279565202751 2116 62-63 0.17137246121231442 2116 64-65 0.028482999846765722 2116 66-67 0.2260571511251399 2116 68-69 0.21584579500675005 2116 70-71 0.05054718145166248 2116 72-73 0.16963382922999415 2116 74-75 0.35959688909338183 2116 76-77 0.5424528170844951 2116 78-79 0.6068675216361541 2116 80-81 0.6860800987579658 2116 82-83 0.5129010139716961 2116 84-85 0.77578212436093 2116 86-87 0.8011633914948355 2116 88-89 0.8706079662395574 2116 90-91 0.893221212341043 2116 92-93 0.9939439560682644 2116 94-95 0.9985058043262107 2116 96-97 1.085173723054499 2116 98-99 1.1537311150143559 2116 100 1.0581857131363108 2201 1 0.9400324379923966 2201 2 0.9454711452097975 2201 3 1.0278208727506701 2201 4 0.918772378919769 2201 5 0.9099942378543986 2201 6 0.929888894432203 2201 7 0.9904585218295097 2201 8 1.0288455032819712 2201 9 1.186092295885075 2201 10-11 1.224195645346832 2201 12-13 0.8902404994405941 2201 14-15 1.1824906941066828 2201 16-17 1.3500502211706404 2201 18-19 1.3569792988767304 2201 20-21 1.3524043791800864 2201 22-23 1.3896429078261079 2201 24-25 1.3917136971070008 2201 26-27 1.3709041795316566 2201 28-29 1.3558401803603388 2201 30-31 1.3620596026610556 2201 32-33 1.3174340837613272 2201 34-35 1.340780871769276 2201 36-37 0.8534355102671753 2201 38-39 1.1892735160985168 2201 40-41 1.176047282128927 2201 42-43 1.255360356668902 2201 44-45 1.3482214374854706 2201 46-47 1.3222814682198205 2201 48-49 1.270050142431856 2201 50-51 1.3791152631671864 2201 52-53 1.340658323375628 2201 54-55 1.2754867005975186 2201 56-57 1.2004001636322528 2201 58-59 1.166095845079191 2201 60-61 1.1948148199995003 2201 62-63 1.082751881029914 2201 64-65 1.0976569024920124 2201 66-67 0.9901094637679435 2201 68-69 0.3825005145371634 2201 70-71 0.7637555637704239 2201 72-73 0.8739340563230655 2201 74-75 0.9031111375968521 2201 76-77 0.8311233087844343 2201 78-79 0.8611666926587063 2201 80-81 0.7681536645221279 2201 82-83 0.820192531028372 2201 84-85 0.7844528316075667 2201 86-87 0.7331932142764543 2201 88-89 0.6755471484921998 2201 90-91 0.6473151590747932 2201 92-93 0.7058044945364195 2201 94-95 0.6302619207075679 2201 96-97 0.6076643586876393 2201 98-99 0.5217521190113921 2201 100 0.4936360508514177 2202 1 0.9559052522562723 2202 2 1.0221768218386735 2202 3 1.0781363066618326 2202 4 0.9781042799842581 2202 5 0.9681272865718853 2202 6 0.9754863743742064 2202 7 1.043753330938003 2202 8 1.0289320498664765 2202 9 1.2452235349026495 2202 10-11 1.2604191488654735 2202 12-13 1.3005258898388803 2202 14-15 1.5112810045650704 2202 16-17 1.4915436962080122 2202 18-19 1.4798504235622332 2202 20-21 1.489637379520758 2202 22-23 1.0113888940928675 2202 24-25 1.229506280028609 2202 26-27 1.3869120977072242 2202 28-29 1.4679939365871348 2202 30-31 1.4904051188128093 2202 32-33 1.4963235108997424 2202 34-35 1.5258764635713362 2202 36-37 1.5225898743220796 2202 38-39 1.5089860514703801 2202 40-41 1.4501718389756206 2202 42-43 0.8035745990137784 2202 44-45 1.2583144036801244 2202 46-47 1.4036224578243832 2202 48-49 1.411259116495458 2202 50-51 1.4853359835419653 2202 52-53 1.4570670405235333 2202 54-55 1.414940653022832 2202 56-57 1.3716955792560626 2202 58-59 1.3428872313802174 2202 60-61 1.303325217733068 2202 62-63 1.319946449042007 2202 64-65 1.2436327816930728 2202 66-67 1.2244586774313362 2202 68-69 1.2094866552684067 2202 70-71 1.1777856939283495 2202 72-73 1.1382003762782702 2202 74-75 1.0158960734368492 2202 76-77 1.0132968456811113 2202 78-79 1.0267092055094125 2202 80-81 0.9806111050668953 2202 82-83 0.9246875381076656 2202 84-85 0.8940421734793773 2202 86-87 0.8787313554908138 2202 88-89 0.8753932714874644 2202 90-91 0.9044847575603434 2202 92-93 0.8628025916274389 2202 94-95 0.8635853751929901 2202 96-97 0.7973573726683405 2202 98-99 0.7463269183001167 2202 100 0.8651524113394373 2203 1 0.03569084375536846 2203 2 0.1774419726663048 2203 3 0.6256498926428122 2203 4 0.6740404700519562 2203 5 0.7565678716464035 2203 6 0.8206353145188174 2203 7 0.852885773323564 2203 8 0.9096873001455492 2203 9 1.086013834424513 2203 10-11 1.109552067893155 2203 12-13 1.166120581225357 2203 14-15 1.404722096749495 2203 16-17 1.3568033800719803 2203 18-19 1.3023172138262353 2203 20-21 1.3453009759442836 2203 22-23 1.369114775454129 2203 24-25 1.388422543636672 2203 26-27 1.3542916831730025 2203 28-29 1.3203234127190555 2203 30-31 1.3271753199325786 2203 32-33 1.3254720326922325 2203 34-35 1.3465547345219093 2203 36-37 1.2980361229154909 2203 38-39 1.3569628910872282 2203 40-41 1.2817906566720012 2203 42-43 1.373888915324116 2203 44-45 1.3688857685526301 2203 46-47 1.3313475523358136 2203 48-49 1.3066066090922703 2203 50-51 1.4594466399347539 2203 52-53 1.3846387632896509 2203 54-55 1.3813478847723601 2203 56-57 1.3175169458471316 2203 58-59 1.2809811541867049 2203 60-61 1.2732036884136093 2203 62-63 1.2183305482305826 2203 64-65 1.2401966891548426 2203 66-67 1.225884429372769 2203 68-69 1.22172050306947 2203 70-71 1.1502030889542638 2203 72-73 1.1234192417516269 2203 74-75 1.0916696524269298 2203 76-77 0.9541455859070993 2203 78-79 0.9327772315776173 2203 80-81 0.9294809834600457 2203 82-83 0.9404926969150367 2203 84-85 0.8861676575188824 2203 86-87 0.9688195024053563 2203 88-89 0.920812687059609 2203 90-91 0.9238205891879687 2203 92-93 0.8702210122579537 2203 94-95 0.8629325294563053 2203 96-97 0.8429059212811651 2203 98-99 0.7714296235086984 2203 100 0.8221064669642679 2204 1 0.9661832329240561 2204 2 1.023142927752307 2204 3 1.07461961124109 2204 4 0.9829051041254075 2204 5 0.9198128656937499 2204 6 0.9309746364591689 2204 7 1.0073200130204256 2204 8 1.0392719653788092 2204 9 1.203788153137431 2204 10-11 1.2222452482477948 2204 12-13 1.2626693324920666 2204 14-15 1.519044411129073 2204 16-17 1.491607232815774 2204 18-19 1.4575089816180977 2204 20-21 1.458647289872502 2204 22-23 1.496729411479862 2204 24-25 1.496498415274111 2204 26-27 1.5102335469282195 2204 28-29 1.506924015059944 2204 30-31 1.4801112661276505 2204 32-33 1.5056951848141438 2204 34-35 1.5348755091146131 2204 36-37 1.5117919516051188 2204 38-39 1.5470431689877628 2204 40-41 1.5075007405698955 2204 42-43 1.5029627157557215 2204 44-45 1.5206663279510906 2204 46-47 1.548224690507574 2204 48-49 1.4985203615527993 2204 50-51 1.6116175397335653 2204 52-53 1.5111796986466857 2204 54-55 1.4283900277915436 2204 56-57 1.3870849319767444 2204 58-59 1.3879723519900793 2204 60-61 1.4389979116891212 2204 62-63 1.4189357558649007 2204 64-65 1.4198727017485808 2204 66-67 1.3571700994980844 2204 68-69 1.3521367268914943 2204 70-71 1.338173837075118 2204 72-73 1.3127119452800997 2204 74-75 1.3137949095665675 2204 76-77 1.2532180661120762 2204 78-79 1.1538861958798066 2204 80-81 1.181245005288499 2204 82-83 1.1890520535966154 2204 84-85 1.1453576579282938 2204 86-87 1.1223610946228 2204 88-89 1.1176405918832124 2204 90-91 1.0574191198275393 2204 92-93 1.0989383116462186 2204 94-95 1.1349563913166314 2204 96-97 1.131205114686999 2204 98-99 1.1018766104315176 2204 100 1.1164974070622442 2205 1 0.9598238969079986 2205 2 1.0075683430981677 2205 3 1.0911479462524554 2205 4 1.0187700934912058 2205 5 0.9931516973143388 2205 6 1.0045212674957398 2205 7 1.0776581568444357 2205 8 1.0440218062071907 2205 9 1.2818680229356971 2205 10-11 1.3257711226129345 2205 12-13 1.364796214531168 2205 14-15 1.6079900055145373 2205 16-17 1.5859492364125813 2205 18-19 1.608349287814299 2205 20-21 1.6350820348066932 2205 22-23 1.6787237068567862 2205 24-25 1.6512669780559577 2205 26-27 1.692759068060674 2205 28-29 1.6581376020818723 2205 30-31 1.6245639672689265 2205 32-33 1.6517346953644392 2205 34-35 1.6714887469556103 2205 36-37 1.6933000137223075 2205 38-39 1.7303578803977544 2205 40-41 1.7111241411448432 2205 42-43 1.7544975726783747 2205 44-45 1.7642830663093463 2205 46-47 1.721108242399879 2205 48-49 1.6682276332993524 2205 50-51 1.6876688960614672 2205 52-53 1.6501780731361535 2205 54-55 1.5850927493152724 2205 56-57 1.5266445754790823 2205 58-59 1.534337850177515 2205 60-61 1.5543545512405714 2205 62-63 1.540080970087459 2205 64-65 1.5156199785101236 2205 66-67 1.4753016141111175 2205 68-69 1.4867533740328085 2205 70-71 1.4456749190061728 2205 72-73 1.2179929913315135 2205 74-75 1.3382464699432859 2205 76-77 1.3319686984209085 2205 78-79 1.287795035144697 2205 80-81 1.3177949056358607 2205 82-83 1.2644416211688245 2205 84-85 1.3120513011393626 2205 86-87 1.3201144328306533 2205 88-89 1.3445905166884664 2205 90-91 1.3569489292934271 2205 92-93 1.3005466712287763 2205 94-95 1.2898549590358606 2205 96-97 1.3008899586273195 2205 98-99 1.2723097876094052 2205 100 1.3102120185768946 2206 1 0.9724478870944111 2206 2 0.9915189704350418 2206 3 1.050783557837164 2206 4 0.9888358300570204 2206 5 0.9934840601133175 2206 6 1.0121956066845144 2206 7 1.0729780253589638 2206 8 1.0542514849361524 2206 9 1.2949844423300902 2206 10-11 1.3334212579376725 2206 12-13 1.3405618225766034 2206 14-15 1.557573610202752 2206 16-17 1.5518869336700192 2206 18-19 1.543101510960902 2206 20-21 1.5438256799073642 2206 22-23 1.6124661020429514 2206 24-25 1.6288893193378016 2206 26-27 1.624999545800108 2206 28-29 1.604463861095958 2206 30-31 1.6305328643576473 2206 32-33 1.6743191217714894 2206 34-35 1.6795884098649339 2206 36-37 1.7052231868006942 2206 38-39 1.6822190315841254 2206 40-41 1.668186191040384 2206 42-43 1.7423857260111433 2206 44-45 1.721601295107476 2206 46-47 1.7197914445304292 2206 48-49 1.747560360415875 2206 50-51 1.7886988167308076 2206 52-53 1.6683570304336897 2206 54-55 1.6162854333657535 2206 56-57 1.5554684271991164 2206 58-59 1.5560717284467458 2206 60-61 1.5116657896115484 2206 62-63 1.5510816707190287 2206 64-65 1.5243696413886099 2206 66-67 1.5089939155042984 2206 68-69 1.4719995352743567 2206 70-71 1.4577049031982412 2206 72-73 1.4349665444576019 2206 74-75 1.4293815494245194 2206 76-77 1.4117311055353738 2206 78-79 1.4159480823390567 2206 80-81 1.4797685801732534 2206 82-83 1.4304700648850392 2206 84-85 1.3725536206388753 2206 86-87 1.4308589566502263 2206 88-89 1.4523408641636522 2206 90-91 1.4113102826664665 2206 92-93 1.444857347323758 2206 94-95 1.4369956161410293 2206 96-97 1.4348012937531216 2206 98-99 1.403514003382103 2206 100 1.470269814331047 2207 1 0.9130029279033494 2207 2 0.962947136113069 2207 3 1.0090209458945907 2207 4 0.902481547108529 2207 5 0.9342244651299509 2207 6 0.9281389907634718 2207 7 0.987882442295593 2207 8 0.9730812874014134 2207 9 1.236565143127585 2207 10-11 1.239433128839103 2207 12-13 1.2325205704626327 2207 14-15 1.4890585342118925 2207 16-17 1.537560512042731 2207 18-19 1.4539360747833072 2207 20-21 1.478225526801367 2207 22-23 1.5057119680383693 2207 24-25 1.5319477576539455 2207 26-27 1.5856266346245604 2207 28-29 1.599687337985543 2207 30-31 1.5976215044435946 2207 32-33 1.6441524891212822 2207 34-35 1.6505833244197774 2207 36-37 1.6868784125743588 2207 38-39 1.6238264329634475 2207 40-41 1.6689891875750789 2207 42-43 1.7360765351244751 2207 44-45 1.7812425308952058 2207 46-47 1.7685718101612409 2207 48-49 1.8103684504375366 2207 50-51 1.7636346984717548 2207 52-53 1.679431834656171 2207 54-55 1.6157231891251982 2207 56-57 1.5733006694695888 2207 58-59 1.6376468503834118 2207 60-61 1.6012329292846985 2207 62-63 1.5672998911835236 2207 64-65 1.5856623812345205 2207 66-67 1.5493798200357745 2207 68-69 1.5303674333078234 2207 70-71 1.548339221702605 2207 72-73 1.4892194156420473 2207 74-75 1.455917514708247 2207 76-77 1.383031885934841 2207 78-79 1.3990876675220818 2207 80-81 1.4204539265143374 2207 82-83 1.3951092945605161 2207 84-85 1.4153404938809544 2207 86-87 1.4248984628656203 2207 88-89 1.4443345614982377 2207 90-91 1.4719105877720509 2207 92-93 1.485967077360879 2207 94-95 1.4625799761391427 2207 96-97 1.4867540064135447 2207 98-99 1.4889144196131419 2207 100 1.6474031631073025 2208 1 0.9688260291729911 2208 2 0.9678149965505476 2208 3 1.0342801553006105 2208 4 0.9070872495351807 2208 5 0.8950003759196221 2208 6 0.9040802306218794 2208 7 0.9761386340129619 2208 8 0.9970787918130881 2208 9 1.1745046962324892 2208 10-11 1.1967030975564583 2208 12-13 1.2229999762412973 2208 14-15 1.4846462952731656 2208 16-17 1.4807455612550342 2208 18-19 1.410460204560799 2208 20-21 1.405821073747049 2208 22-23 1.5064498038089482 2208 24-25 1.486653877253957 2208 26-27 1.5465779514738216 2208 28-29 1.5441012721434149 2208 30-31 1.5595702492524453 2208 32-33 1.5503887817669053 2208 34-35 1.520290327948011 2208 36-37 1.5205748645653543 2208 38-39 1.5478938181333817 2208 40-41 1.6384652593008227 2208 42-43 1.632164939757601 2208 44-45 1.6626030294359566 2208 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1.3980696228599498 2311 78-79 1.430965661795284 2311 80-81 1.4048231582566153 2311 82-83 1.398700380575054 2311 84-85 1.4250439744953631 2311 86-87 1.456024685514798 2311 88-89 1.5153060045854794 2311 90-91 1.564748776779414 2311 92-93 1.5620309558393188 2311 94-95 1.6862501657678841 2311 96-97 1.7360206163156526 2311 98-99 1.7124742497203478 2311 100 1.745133569726061 2312 1 0.7207779956188212 2312 2 0.7210489987598905 2312 3 0.7931520719005896 2312 4 0.6876822250205876 2312 5 0.7479411999819376 2312 6 0.5723425151274171 2312 7 0.7062272217919627 2312 8 0.7774522301783833 2312 9 0.9319772915095186 2312 10-11 0.9861803805785527 2312 12-13 1.0849727587799052 2312 14-15 1.2837624170170443 2312 16-17 1.305945490385227 2312 18-19 1.2243937024636793 2312 20-21 1.2679772898995765 2312 22-23 1.2601258970904965 2312 24-25 1.3134048343616271 2312 26-27 1.3236512906489608 2312 28-29 1.3780842599149068 2312 30-31 1.2733617291746526 2312 32-33 1.3786364321358135 2312 34-35 1.4583720205782313 2312 36-37 1.5308782916643082 2312 38-39 1.5302586926711896 2312 40-41 1.5578623801660854 2312 42-43 1.6116031146545637 2312 44-45 1.6141473948541218 2312 46-47 1.5863357598910994 2312 48-49 1.6744449516400124 2312 50-51 1.6292121957980328 2312 52-53 1.4764142180287436 2312 54-55 1.4113802734714866 2312 56-57 1.4019991800366398 2312 58-59 1.4203763014026478 2312 60-61 1.4197207628983435 2312 62-63 1.4181714888165473 2312 64-65 1.447145290547084 2312 66-67 1.4158637075041511 2312 68-69 1.4376166809765252 2312 70-71 1.4602756011348248 2312 72-73 1.4659379535729613 2312 74-75 1.4984459111228432 2312 76-77 1.4172825163819098 2312 78-79 1.4715485452495756 2312 80-81 1.3448108402439622 2312 82-83 1.44415637312866 2312 84-85 1.4939378374236298 2312 86-87 1.5587852457673037 2312 88-89 1.6328880403308794 2312 90-91 1.6772878531597364 2312 92-93 1.7378510603133712 2312 94-95 1.7290370181557826 2312 96-97 1.7383205751860196 2312 98-99 1.628032829289939 2312 100 1.7569531626991584 2313 1 0.6508422289216789 2313 2 0.7145670614459689 2313 3 0.6950626610184365 2313 4 0.6301289512319954 2313 5 0.6487745919222974 2313 6 0.7089918341137462 2313 7 0.7221659891689498 2313 8 0.7397022712033063 2313 9 0.9175962989408859 2313 10-11 0.7767206201640846 2313 12-13 0.9752006229053691 2313 14-15 1.1482723450476087 2313 16-17 1.1237450309061217 2313 18-19 1.1490696055979797 2313 20-21 1.1288641314496175 2313 22-23 1.178022725278133 2313 24-25 1.2769160986324408 2313 26-27 1.2652915841529548 2313 28-29 1.24301322819678 2313 30-31 1.252106718273744 2313 32-33 1.312074085979262 2313 34-35 1.2621252146612676 2313 36-37 1.2741982625952275 2313 38-39 1.3029191001072462 2313 40-41 1.3078166666841184 2313 42-43 1.3799230855854745 2313 44-45 1.385826134568589 2313 46-47 1.350230314126044 2313 48-49 1.4576429027383 2313 50-51 1.4021791179913379 2313 52-53 1.1939365473064711 2313 54-55 1.1779250375195929 2313 56-57 1.1970685707579705 2313 58-59 1.2426522467340178 2313 60-61 1.201771188385365 2313 62-63 1.232277028352847 2313 64-65 1.2272353109423264 2313 66-67 1.2342411133226605 2313 68-69 1.234641202157107 2313 70-71 1.2218358374383627 2313 72-73 1.2823414746832142 2313 74-75 1.2862708870939628 2313 76-77 1.3083496331505557 2313 78-79 1.3126742690463686 2313 80-81 1.3141769466274376 2313 82-83 1.301463497399002 2313 84-85 1.3550051886203853 2313 86-87 1.4136390798390863 2313 88-89 1.460316129507337 2313 90-91 1.504602830771848 2313 92-93 1.6044819766928633 2313 94-95 1.6466290897970133 2313 96-97 1.6573602404226762 2313 98-99 1.6997637292594625 2313 100 1.8515495947032825 2314 1 0.46772933728783883 2314 2 0.4983180464371486 2314 3 0.5606670962600049 2314 4 0.4586073318545303 2314 5 0.4579861986310547 2314 6 0.48915841358920886 2314 7 0.5833816270756031 2314 8 0.5613184343359379 2314 9 0.7695206647066541 2314 10-11 0.702841556486014 2314 12-13 0.46228948171682305 2314 14-15 0.8470464809404064 2314 16-17 0.8388234606482996 2314 18-19 0.9070715449768088 2314 20-21 0.9047506523818569 2314 22-23 0.8321458588954869 2314 24-25 0.8089526119234236 2314 26-27 0.933699919247438 2314 28-29 0.9839548990112874 2314 30-31 0.9122031425103643 2314 32-33 0.9636431812578792 2314 34-35 1.0193715065327709 2314 36-37 1.0850980104143844 2314 38-39 1.0945181599050144 2314 40-41 1.1149845634589752 2314 42-43 1.1339410219524453 2314 44-45 1.2258914760058914 2314 46-47 1.2288030267811578 2314 48-49 1.2906159708563862 2314 50-51 1.2339419894730383 2314 52-53 1.0818155547818193 2314 54-55 1.0643834634815548 2314 56-57 1.0611376034821944 2314 58-59 1.0451302766669741 2314 60-61 1.0186731750745608 2314 62-63 1.0014401929355827 2314 64-65 0.992532517474622 2314 66-67 1.0477166780348952 2314 68-69 1.0514387711199618 2314 70-71 1.0401710801244022 2314 72-73 1.1009301011772479 2314 74-75 1.1468761576773403 2314 76-77 1.1932362114403645 2314 78-79 1.188566083043085 2314 80-81 1.1942736865231716 2314 82-83 1.0966990175956077 2314 84-85 1.0658637082792097 2314 86-87 1.294490081421614 2314 88-89 1.3922635075926628 2314 90-91 1.4554414061637573 2314 92-93 1.4696569423071892 2314 94-95 1.5442079266239617 2314 96-97 1.5457781299797126 2314 98-99 1.6414361701782774 2314 100 1.6298339412061473 2315 1 0.43016540049018204 2315 2 0.4625236219117639 2315 3 0.5043187945420904 2315 4 0.4665959955704011 2315 5 0.36538878313748313 2315 6 -0.2887193650100528 2315 7 0.3084849892158559 2315 8 0.3575626596964696 2315 9 0.5847031795768629 2315 10-11 0.6801073773530888 2315 12-13 0.7139910681192205 2315 14-15 0.8362656645525988 2315 16-17 0.7400680795171581 2315 18-19 0.8353649756664652 2315 20-21 0.9064018556466849 2315 22-23 0.7853210597288651 2315 24-25 0.8914253424410816 2315 26-27 0.9557083750154121 2315 28-29 0.9841652945378669 2315 30-31 0.9820149794715434 2315 32-33 1.0327827609784777 2315 34-35 1.0657922609809773 2315 36-37 1.1436716520636026 2315 38-39 1.1317461659603012 2315 40-41 1.2036540901015798 2315 42-43 1.2086094059258201 2315 44-45 1.2025977958434808 2315 46-47 1.2465136914705752 2315 48-49 1.2553720720090737 2315 50-51 1.245828393751438 2315 52-53 1.0237431427801837 2315 54-55 0.9377614166726502 2315 56-57 0.9350738582205906 2315 58-59 0.8866470344444437 2315 60-61 0.8074095678408639 2315 62-63 1.0204155076020243 2315 64-65 1.033301397377933 2315 66-67 1.09067141756082 2315 68-69 1.1678344237040292 2315 70-71 1.134339978511953 2315 72-73 1.1767041878592792 2315 74-75 1.1980317837627794 2315 76-77 1.2136332642769787 2315 78-79 1.1365510365235991 2315 80-81 1.129869015083866 2315 82-83 1.3214089911096742 2315 84-85 1.2049713898842853 2315 86-87 1.2491798202819808 2315 88-89 1.3931553311462217 2315 90-91 1.4652478228335148 2315 92-93 1.5502934610371994 2315 94-95 1.6804931142766222 2315 96-97 1.7036940317871583 2315 98-99 1.7751976351025966 2315 100 1.8088951235529827 2316 1 -0.05707556290317228 2316 2 -0.12771773393287233 2316 3 -0.00828994624288626 2316 4 -0.04880091303861889 2316 5 -0.03416011662473295 2316 6 0.07428768951915998 2316 7 0.07245279585467301 2316 8 0.12344384702334565 2316 9 0.18784535932397972 2316 10-11 0.060883394410673475 2316 12-13 0.12540556872441044 2316 14-15 0.3433340717025146 2316 16-17 0.39427873904317323 2316 18-19 0.3364379706650382 2316 20-21 0.2563229667991891 2316 22-23 0.13882984673658427 2316 24-25 0.19572461825175225 2316 26-27 0.2579090194698068 2316 28-29 0.42561715503760666 2316 30-31 0.39710552581044567 2316 32-33 0.48734235498206147 2316 34-35 0.5120065368427689 2316 36-37 0.5638941299534679 2316 38-39 0.6289300251947125 2316 40-41 0.5929248862518506 2316 42-43 0.6542267396444501 2316 44-45 0.4650947796342848 2316 46-47 0.5235905196995745 2316 48-49 0.5511675568934038 2316 50-51 0.3355214112439029 2316 52-53 0.13527385119488855 2316 54-55 0.08024960798613989 2316 56-57 0.2094257159576216 2316 58-59 0.2596803383125703 2316 60-61 0.315869635392211 2316 62-63 0.35446760503617014 2316 64-65 0.33829265940575937 2316 66-67 0.40089325946998144 2316 68-69 0.42066715096650853 2316 70-71 0.2956504077047484 2316 72-73 0.36649002676120546 2316 74-75 0.4840557479220351 2316 76-77 0.5299769941033645 2316 78-79 0.5978239194309829 2316 80-81 0.7409399434671755 2316 82-83 0.756706220060515 2316 84-85 0.770149815436227 2316 86-87 0.8552065930419275 2316 88-89 0.9315225950827788 2316 90-91 0.97026404368086 2316 92-93 1.0295646597860149 2316 94-95 1.0495600663755873 2316 96-97 1.1611343092230193 2316 98-99 1.2373874661199302 2316 100 1.1108393958908032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 71.0 1 706.0 2 334.0 3 260.0 4 202.0 5 162.0 6 225.0 7 974.0 8 3288.0 9 6671.0 10 11240.0 11 19419.0 12 29858.0 13 38898.0 14 45348.0 15 52798.0 16 62020.0 17 73655.0 18 86291.0 19 100593.0 20 117375.0 21 135635.0 22 146314.0 23 150522.0 24 154200.0 25 165028.0 26 183348.0 27 209384.0 28 241627.0 29 280969.0 30 330627.0 31 399744.0 32 490507.0 33 620218.0 34 824444.0 35 1096591.0 36 1550792.0 37 2107810.0 38 1897700.0 39 484275.0 40 464.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78797164717528 19.489201656505614 13.01208080905634 23.71074588726276 2 15.632666632074042 20.394947537638487 40.47118296535703 23.50120286493045 3 18.465889257001216 26.405562187739267 29.93499249608712 25.193556059172394 4 12.353030398557099 17.120755793207554 39.06134209707165 31.4648717111637 5 13.125021405443235 37.83172660122 35.14732102883122 13.895930964505546 6 30.09493168267105 37.86671095788229 18.446157610466905 13.592199748979755 7 26.61972641430227 32.574194952904676 23.157805750142387 17.648272882650662 8 25.528351190970017 35.22346501302433 20.70190645182194 18.54627734418371 9 26.437673224393986 15.447742782817311 20.778400959371883 37.336183033416816 10-11 24.578661945086456 26.109311207212983 28.889684678352808 20.422342169347758 12-13 25.370348497405743 24.083108021162793 28.5371534589865 22.009390022444965 14-15 22.731523511948073 25.478067550863138 26.297896032817402 25.492512904371388 16-17 21.963907448988266 28.10954350820124 27.124668579477795 22.801880463332697 18-19 21.691436938451098 27.65047079325783 29.01713673512677 21.640955533164302 20-21 22.703159989595548 26.904964792544707 28.693836747789714 21.69803847007003 22-23 22.64807752773475 26.781774578356703 28.38651962827224 22.183628265636308 24-25 22.300738875167355 26.86326652300211 28.71684379642314 22.119150805407394 26-27 22.238955578879562 26.977238408054284 28.469744252786537 22.314061760279618 28-29 21.923854849335466 27.40298145852333 28.50131539060856 22.17184830153264 30-31 22.544097773406854 27.034289562485164 28.43723906047868 21.984373603629304 32-33 22.47346651181526 27.20459622479136 28.09552410607437 22.226413157319012 34-35 22.557840712434174 27.155916361112553 28.434956380654814 21.851286545798455 36-37 22.46671992024095 26.934016444733448 28.391640137878316 22.207623497147285 38-39 22.36558005746307 26.95649816598799 28.55992591330893 22.117995863240008 40-41 22.37028519244539 26.600478669190224 28.788234457703492 22.241001680660897 42-43 22.182714505964093 26.78051915029107 28.39715580756276 22.63961053618207 44-45 22.044231319776515 27.12598521220636 28.484927346358113 22.344856121659014 46-47 22.09836238235489 26.87944932570579 28.18812041695618 22.83406787498314 48-49 22.278035597899216 26.849329348765096 28.175894589233057 22.69674046410263 50-51 22.10870832025605 26.999937361079212 28.010557340320062 22.880796978344677 52-53 21.83217906404747 27.221093960124026 27.966254570018645 22.980472405809856 54-55 21.811280930271025 27.06596963856937 28.089366483437615 23.033382947721982 56-57 22.143063584948187 26.91433975064307 27.885047973303777 23.057548691104966 58-59 21.89165729892708 26.962085902883974 28.122274105405115 23.02398269278383 60-61 21.98291609322405 27.044106825417536 27.859697169481457 23.113279911876962 62-63 22.011180998945115 26.753571765305896 27.873289164185564 23.36195807156343 64-65 21.66066706958783 26.6869428413815 27.984933823974504 23.66745626505616 66-67 21.900585639937077 26.602075159326084 27.78885435425274 23.708484846484097 68-69 21.660651502954583 26.635691293740205 27.90667722169467 23.796979981610548 70-71 21.65004630146062 26.32612446890745 28.023441633644453 24.000387595987473 72-73 21.777986495837016 26.39338907970936 28.019293406553587 23.809331017900035 74-75 21.821260913822123 26.528312650900173 27.872163619370166 23.778262815907535 76-77 21.73388020297062 26.3357364608443 27.848369559589003 24.082013776596074 78-79 21.614531984389817 26.32511090082435 27.983757719941355 24.076599394844482 80-81 21.62859249406141 26.39219956541478 28.019259627307054 23.95994831321676 82-83 21.87260046612383 26.053141879773122 28.32683857778345 23.747419076319588 84-85 21.970301917237062 26.13717194691947 27.86732053635027 24.025205599493198 86-87 21.641512986020924 26.27607020435087 28.126793809571804 23.9556230000564 88-89 21.952500081797957 25.72752215899402 28.05429750368318 24.26568025552485 90-91 22.092794788383287 25.631446987945942 28.146573254237712 24.129184969433062 92-93 22.200769508484193 25.662208468719378 28.03727061736036 24.09975140543607 94-95 21.837170404653968 25.50029865497156 28.209649683247477 24.452881257126993 96-97 21.963533330579754 25.301569618995444 28.134648576278792 24.600248474146007 98-99 21.936490810977567 25.395789913349372 28.000735172963097 24.666984102709968 100 22.294532941537174 25.102029447434543 27.852365859264523 24.751071751763764 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 849.0 1 659.0 2 787.0 3 1144.5 4 998.5 5 927.5 6 1328.0 7 2349.5 8 3859.5 9 4853.0 10 4832.0 11 4805.0 12 5429.5 13 6331.0 14 7228.0 15 8232.5 16 9408.5 17 10756.0 18 11967.0 19 13150.5 20 14909.0 21 17234.5 22 20342.0 23 24792.0 24 30788.0 25 38536.5 26 49003.5 27 61567.0 28 77408.0 29 99146.0 30 124344.5 31 150103.0 32 181754.5 33 211499.5 34 239033.0 35 268137.5 36 298800.5 37 338299.5 38 361050.5 39 363618.0 40 366977.0 41 373086.0 42 385198.5 43 404415.5 44 427133.5 45 456199.5 46 484468.5 47 505460.5 48 520692.0 49 519143.5 50 504389.5 51 496086.5 52 484154.5 53 461806.0 54 434774.5 55 391517.0 56 347253.0 57 300335.5 58 252419.0 59 216555.0 60 176971.0 61 138220.5 62 107507.5 63 82352.0 64 60147.5 65 41109.0 66 28816.5 67 21537.0 68 16217.0 69 12079.0 70 9131.5 71 6959.0 72 5177.0 73 3572.0 74 2499.0 75 1798.5 76 1256.0 77 847.5 78 593.0 79 435.5 80 317.5 81 236.0 82 187.0 83 141.5 84 102.0 85 84.5 86 80.0 87 60.5 88 39.5 89 45.0 90 44.0 91 36.0 92 26.0 93 17.5 94 13.5 95 8.5 96 7.5 97 4.5 98 3.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2202451085908628 2 0.14698133019465145 3 0.02400048776515527 4 0.022894930748816045 5 0.027572922004520075 6 0.05629265315285473 7 0.06270323376252322 8 0.05545936017785277 9 0.042572195554555235 10-11 0.0448328121402041 12-13 0.042687701511486195 14-15 0.020654940226904853 16-17 0.018951227362173136 18-19 0.01867483810808833 20-21 0.022267898411190812 22-23 0.02571657626812959 24-25 0.022899055961563577 26-27 0.019883525443115915 28-29 0.01916986363779246 30-31 0.01747440119855581 32-33 0.026104346266397824 34-35 0.02687163583743923 36-37 0.025497939992510266 38-39 0.02385198010624403 40-41 0.025019415313796273 42-43 0.031566127944133396 44-45 0.0411407467311608 46-47 0.04578161107213702 48-49 0.04135525779403258 50-51 0.029041497742642336 52-53 0.044110899909385576 54-55 0.042489691299604546 56-57 0.04507619969230863 58-59 0.04844237329429672 60-61 0.024619269677285432 62-63 0.026025967224194674 64-65 0.026776755944245934 66-67 0.03239942091913535 68-69 0.036322498242040584 70-71 0.04024970077769336 72-73 0.04422228065356901 74-75 0.025555692970975746 76-77 0.018823345766999566 78-79 0.018270567258829955 80-81 0.021413979372451188 82-83 0.019371999062421645 84-85 0.012000243882577635 86-87 0.013625577705106195 88-89 0.01908735938284177 90-91 0.020032033102027152 92-93 0.020312547568859494 94-95 0.024132494573076372 96-97 0.024986413611815996 98-99 0.030613203799452947 100 0.030873092202547615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2120587E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.05545029185965 #Duplication Level Percentage of deduplicated Percentage of total 1 74.9565031485194 46.51459555184567 2 14.653709788332916 18.18685118722461 3 4.92145272921545 9.162088956046992 4 2.1923768334487828 5.441957264364223 5 1.0903195986034648 3.383013682668884 6 0.6121031270413765 2.2790601104164794 7 0.37387899430964117 1.624086054258669 8 0.2573064567391722 1.277381442876179 9 0.1818163734829699 1.0154427234226766 >10 0.7195803860371423 7.412882024113249 >50 0.029395276217137018 1.2347759283051998 >100 0.01060886490537687 1.121749694454811 >500 5.520704759593999E-4 0.22951843209152245 >1k 3.4465454965164344E-4 0.3869031844105364 >5k 0.0 0.0 >10k+ 5.1698121392323214E-5 0.7296937635003613 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30004 0.2475457665540456 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24028 0.1982412237955142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14203 0.11718079330646279 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.250425495068845E-6 0.0 0.0 0.0 1.650085099013769E-5 2 8.250425495068845E-6 0.0 0.0 0.0 1.650085099013769E-5 3 8.250425495068845E-6 0.0 0.0 8.250425495068845E-6 1.650085099013769E-5 4 8.250425495068845E-6 0.0 0.0 2.4751276485206533E-5 1.650085099013769E-5 5 8.250425495068845E-6 0.0 0.0 3.300170198027538E-5 3.300170198027538E-5 6 1.650085099013769E-5 0.0 0.0 3.300170198027538E-5 4.125212747534422E-5 7 1.650085099013769E-5 0.0 0.0 4.9502552970413065E-5 4.125212747534422E-5 8 2.4751276485206533E-5 0.0 0.0 4.9502552970413065E-5 6.600340396055076E-5 9 2.4751276485206533E-5 0.0 0.0 9.900510594082613E-5 6.600340396055076E-5 10-11 2.8876489232740954E-5 0.0 0.0 1.6088329715384248E-4 7.42538294556196E-5 12-13 3.300170198027538E-5 0.0 0.0 1.9801021188165226E-4 2.268867011143932E-4 14-15 3.71269147278098E-5 0.0 0.0 2.1451106287178996E-4 2.846396795798751E-4 16-17 5.3627765717947484E-5 0.0 0.0 2.6401361584220303E-4 3.547682962879603E-4 18-19 5.775297846548191E-5 8.250425495068845E-6 0.0 3.5064308354042586E-4 3.630187217830292E-4 20-21 5.775297846548191E-5 8.250425495068845E-6 0.0 5.610289336646815E-4 4.2902212574357987E-4 22-23 7.42538294556196E-5 8.250425495068845E-6 0.0 0.0010931813780966219 4.413977639861832E-4 24-25 8.250425495068844E-5 8.250425495068845E-6 0.0 0.002570007541713945 4.455229767337176E-4 26-27 9.48798931932917E-5 8.250425495068845E-6 0.0 0.005428779975755299 4.6202382772385527E-4 28-29 1.0313031868836056E-4 8.250425495068845E-6 0.0 0.017193886731723472 4.6202382772385527E-4 30-31 1.0725553143589498E-4 8.250425495068845E-6 0.0 0.05918030207612882 4.7027425321892415E-4 32-33 1.2375638242603267E-4 8.250425495068845E-6 0.0 0.15261224559503594 5.115263806942683E-4 34-35 1.5263287165877363E-4 8.250425495068845E-6 0.0 0.29319949603100903 5.445280826745437E-4 36-37 1.815093608915146E-4 8.250425495068845E-6 0.0 0.5168231538621025 5.445280826745437E-4 38-39 1.815093608915146E-4 1.2375638242603268E-5 0.0 0.8402934610345192 5.899054228974223E-4 40-41 1.9801021188165226E-4 1.650085099013769E-5 0.0 1.1824509819532667 6.022810611400256E-4 42-43 1.9801021188165226E-4 1.650085099013769E-5 0.0 1.5225788982002273 6.105314866350945E-4 44-45 1.9801021188165226E-4 1.650085099013769E-5 0.0 1.922613153966883 6.476584013629042E-4 46-47 1.9801021188165226E-4 1.650085099013769E-5 0.0 2.39296578622801 6.971609543333173E-4 48-49 1.9801021188165226E-4 1.650085099013769E-5 0.0 2.88514904434909 7.549139327987992E-4 50-51 1.9801021188165226E-4 1.650085099013769E-5 0.0 3.3882558658256405 7.92040847526609E-4 52-53 2.227614883668588E-4 1.650085099013769E-5 0.0 3.869672318675655 8.621694642346942E-4 54-55 2.227614883668588E-4 1.650085099013769E-5 0.0 4.379499111717939 8.99296378962504E-4 56-57 2.227614883668588E-4 1.650085099013769E-5 0.0 4.961430498374378 9.240476554477105E-4 58-59 2.227614883668588E-4 1.650085099013769E-5 0.0 5.602793825084545 9.694249956705891E-4 60-61 2.227614883668588E-4 1.650085099013769E-5 0.0 6.249086781028014 9.900510594082613E-4 62-63 2.227614883668588E-4 1.650085099013769E-5 0.0 6.8861887629699785 0.0010478040378737433 64-65 2.227614883668588E-4 1.650085099013769E-5 0.0 7.505296566907196 0.0010725553143589497 66-67 2.3101191386192763E-4 1.650085099013769E-5 0.0 8.18353929558032 0.0010808057398540185 68-69 2.3926233935699648E-4 1.650085099013769E-5 0.0 8.914477491890452 0.0010890561653490874 70-71 2.433875521045309E-4 1.650085099013769E-5 0.0 9.67353726350052 0.0011055570163392252 72-73 2.4751276485206533E-4 1.650085099013769E-5 0.0 10.413022900623542 0.0011798108457948446 74-75 2.4751276485206533E-4 1.650085099013769E-5 0.0 11.122592494901443 0.0012251881860177232 76-77 2.4751276485206533E-4 1.650085099013769E-5 0.0 11.870518317305919 0.0012664403134930676 78-79 2.4751276485206533E-4 1.650085099013769E-5 0.0 12.660475932395023 0.0013035672282208774 80-81 2.5163797759959977E-4 1.650085099013769E-5 0.0 13.476240878432703 0.0013324437174536184 82-83 2.598884030946686E-4 1.650085099013769E-5 0.0 14.29060737734897 0.001386071483171566 84-85 2.6401361584220303E-4 2.4751276485206533E-5 0.0 15.06392388421452 0.0013901966959191004 86-87 2.6401361584220303E-4 2.4751276485206533E-5 0.0 15.830124399090572 0.0014520748871321167 88 2.6401361584220303E-4 2.4751276485206533E-5 0.0 16.42238119325409 0.0015180782910926673 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17510 0.0 49.511444 1 GTATCAA 32890 0.0 42.360527 1 ATCAACG 38720 0.0 34.953117 3 CAACGCA 38810 0.0 34.84769 5 TATCAAC 40620 0.0 33.893345 2 AACGCAG 41870 0.0 32.727814 6 TCAACGC 41525 0.0 32.626007 4 ACGCAGA 45995 0.0 29.209976 7 CGCAGAG 46665 0.0 28.68831 8 GTACTAG 5165 0.0 26.272179 1 TACCTGG 8035 0.0 25.540483 2 GCAGAGT 50800 0.0 25.474203 9 GTACATG 39080 0.0 25.306484 1 TACATGG 39725 0.0 24.32504 2 ACATGGG 39205 0.0 23.897114 3 CATGGGG 28785 0.0 22.07954 4 GCATCGT 5460 0.0 21.43695 8 GTGGTAT 7950 0.0 21.158018 1 GAGTACT 29465 0.0 21.151335 12-13 CAGAGTA 49680 0.0 20.806541 10-11 >>END_MODULE