FastQCFastQC Report
Mon 6 Jun 2016
ERR1211414_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211414_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12120587
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283210.23366030044584477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT236580.19518856636233872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140400.11583597395076657No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA186450.049.4276051
GTATCAA326700.039.175531
ATCAACG389400.031.945643
CAACGCA390800.031.8011135
TATCAAC410250.030.8254762
TCAACGC417950.029.831284
AACGCAG425250.029.7655376
GTACTAG50000.027.5532931
ACGCAGA464500.026.6333377
CGCAGAG471100.026.1505018
TACCTGG84200.024.5575622
GTACATG395850.023.613571
TACATGG397400.022.988632
GCAGAGT525450.022.9268979
ACATGGG389150.022.4258293
CTAACGC20600.021.672553
GTGGTAT85900.021.62121
TCTAACG20800.021.4637172
CTGTGCG63050.020.8656359
TAACGCC21000.020.8124244
Produced by FastQC (version 0.11.5)