##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211414_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12120587 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09763528779588 33.0 31.0 34.0 30.0 34.0 2 32.24123724370775 34.0 31.0 34.0 30.0 34.0 3 32.31974078483162 34.0 31.0 34.0 30.0 34.0 4 35.95133758785775 37.0 35.0 37.0 35.0 37.0 5 35.80929281725382 37.0 35.0 37.0 35.0 37.0 6 35.841931913033584 37.0 35.0 37.0 35.0 37.0 7 35.77088114626792 37.0 35.0 37.0 33.0 37.0 8 35.75420711884664 37.0 35.0 37.0 33.0 37.0 9 37.34851554631801 39.0 37.0 39.0 34.0 39.0 10-11 37.32826549572228 39.0 37.0 39.0 34.0 39.0 12-13 37.25197773012149 39.0 37.0 39.0 33.5 39.0 14-15 38.48083681920686 40.0 38.0 41.0 34.0 41.0 16-17 38.476382538238454 40.0 38.0 41.0 34.0 41.0 18-19 38.47539318846521 40.0 38.0 41.0 34.0 41.0 20-21 38.43498747214141 40.0 38.0 41.0 34.0 41.0 22-23 38.33845633878953 40.0 38.0 41.0 34.0 41.0 24-25 38.226863682427265 40.0 38.0 41.0 33.5 41.0 26-27 38.110490028246986 40.0 37.5 41.0 33.0 41.0 28-29 38.045268269597834 40.0 37.0 41.0 33.0 41.0 30-31 38.09812924077027 40.0 37.5 41.0 33.0 41.0 32-33 37.95998188866595 40.0 37.0 41.0 33.0 41.0 34-35 37.86034983289176 40.0 37.0 41.0 33.0 41.0 36-37 37.72563552408806 40.0 37.0 41.0 33.0 41.0 38-39 37.61448084156321 40.0 37.0 41.0 32.5 41.0 40-41 37.55617669342252 40.0 37.0 41.0 32.0 41.0 42-43 37.43354047126596 40.0 36.5 41.0 32.0 41.0 44-45 37.28452079094849 40.0 36.0 41.0 31.5 41.0 46-47 37.07364762944236 39.5 36.0 41.0 31.0 41.0 48-49 36.86603454106637 39.0 36.0 41.0 31.0 41.0 50-51 36.68986790821269 39.0 35.0 41.0 30.0 41.0 52-53 36.48923505107467 39.0 35.0 40.5 30.0 41.0 54-55 36.29490943796699 39.0 35.0 40.0 29.5 41.0 56-57 36.07003068415746 38.0 35.0 40.0 29.0 41.0 58-59 35.8065556973437 38.0 34.5 40.0 28.5 41.0 60-61 35.55948309269179 38.0 34.0 40.0 28.0 41.0 62-63 35.27749047962776 37.0 34.0 40.0 28.0 41.0 64-65 34.937250935123856 37.0 34.0 40.0 27.5 41.0 66-67 34.589582171226525 36.0 33.5 39.0 26.5 41.0 68-69 34.232115532028274 36.0 33.0 39.0 26.0 40.0 70-71 33.874170904428965 35.0 33.0 38.5 26.0 40.0 72-73 33.5311218012791 35.0 32.5 38.0 26.0 40.0 74-75 33.17000525634608 35.0 32.0 37.0 25.5 39.0 76-77 32.15283546085681 34.0 30.5 36.0 24.5 39.0 78-79 32.62495422045153 35.0 32.0 36.0 25.0 39.0 80-81 32.510791391539044 35.0 32.0 36.0 25.0 37.5 82-83 32.237858405702625 35.0 32.0 35.0 25.0 37.0 84-85 31.9652130709511 34.0 31.5 35.0 25.0 37.0 86-87 31.708504258085853 34.0 31.0 35.0 24.0 36.0 88-89 31.477207168266684 34.0 31.0 35.0 24.0 36.0 90-91 31.259164552013857 34.0 31.0 35.0 23.0 35.5 92-93 31.082167637590487 34.0 31.0 35.0 23.0 35.0 94-95 30.91528537355493 34.0 30.5 35.0 22.0 35.0 96-97 30.744788144336574 34.0 30.0 35.0 20.0 35.0 98-99 30.553804902353328 34.0 30.0 35.0 20.0 35.0 100 28.75970553241357 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26278887862657285 1101 2 -0.24573467071703448 1101 3 -0.288673669445199 1101 4 -0.18090089563843748 1101 5 -0.19107062450708412 1101 6 -0.282845498282839 1101 7 -0.2613903363510346 1101 8 -0.3261973186351952 1101 9 -0.48895880876467857 1101 10-11 -0.41289238975274856 1101 12-13 -0.4556192360330087 1101 14-15 -0.5828723916532823 1101 16-17 -0.5727041574646634 1101 18-19 -0.6055799738909826 1101 20-21 -0.6354181016232161 1101 22-23 -0.7263436359645397 1101 24-25 -0.7272487474117142 1101 26-27 -0.6824266202224578 1101 28-29 -0.7055724712219344 1101 30-31 -0.652035058510883 1101 32-33 -0.5525542391540057 1101 34-35 -0.6342162972571828 1101 36-37 -0.641850319266176 1101 38-39 -0.6861935793775729 1101 40-41 -0.7528248024322366 1101 42-43 -0.7014770143952731 1101 44-45 -0.8084627618949582 1101 46-47 -0.8254445944245958 1101 48-49 -0.7565619949602649 1101 50-51 -0.8448062117372785 1101 52-53 -0.8343274954838691 1101 54-55 -0.8042523151288634 1101 56-57 -0.8298433050433758 1101 58-59 -0.7228671140444831 1101 60-61 -0.7202441176153727 1101 62-63 -0.7054995266041857 1101 64-65 -0.7733602208450847 1101 66-67 -0.8563893123415198 1101 68-69 -0.8148655860263929 1101 70-71 -0.8641396081298183 1101 72-73 -0.8661297708089748 1101 74-75 -0.8919871381942173 1101 76-77 -0.7328140684400424 1101 78-79 -0.6260202467501088 1101 80-81 -0.5775659012371435 1101 82-83 -0.7048672290548481 1101 84-85 -0.808656181492772 1101 86-87 -0.8597295829025562 1101 88-89 -0.8728620429376299 1101 90-91 -1.0062215920069377 1101 92-93 -1.084409219000026 1101 94-95 -1.0437216454906135 1101 96-97 -1.0439561226981056 1101 98-99 -1.1095281682680174 1101 100 -1.0317260997251871 1102 1 -0.35502736442294847 1102 2 -0.3002831690285035 1102 3 -0.34499168318778217 1102 4 -0.21994602301246857 1102 5 -0.22388320646427218 1102 6 -0.2951222989879625 1102 7 -0.2680644150113096 1102 8 -0.3239461706830795 1102 9 -0.4216699660209997 1102 10-11 -0.4366369955910656 1102 12-13 -0.43609559388131913 1102 14-15 -0.6066220221565715 1102 16-17 -0.6510254242488784 1102 18-19 -0.68122365798515 1102 20-21 -0.7280619920963076 1102 22-23 -0.7617969612388009 1102 24-25 -0.7104397969392267 1102 26-27 -0.8325023823660871 1102 28-29 -0.7727654531620729 1102 30-31 -0.6939260090647466 1102 32-33 -0.7271318364033306 1102 34-35 -0.7465502565902469 1102 36-37 -0.725635235172895 1102 38-39 -0.6857996701539051 1102 40-41 -0.8031634403411587 1102 42-43 -0.7961703554151214 1102 44-45 -0.7516988765396775 1102 46-47 -0.9050855833374882 1102 48-49 -0.8689193373683821 1102 50-51 -0.9205490655607065 1102 52-53 -0.9619631528595676 1102 54-55 -1.028449238857398 1102 56-57 -0.9711115157773307 1102 58-59 -0.971885171955094 1102 60-61 -1.0349381988541566 1102 62-63 -1.0337457175404765 1102 64-65 -1.0019869137282527 1102 66-67 -0.9457232231436663 1102 68-69 -0.992168684112265 1102 70-71 -1.0566113364233942 1102 72-73 -1.0979859751917544 1102 74-75 -1.0820471988725657 1102 76-77 -0.9352214467071178 1102 78-79 -0.8041374876256846 1102 80-81 -0.6981940873036692 1102 82-83 -0.6803485061727201 1102 84-85 -0.7983942469330287 1102 86-87 -0.9111765393526774 1102 88-89 -0.8625327043355036 1102 90-91 -0.8712484286200279 1102 92-93 -0.8913466391262332 1102 94-95 -0.9234681768785933 1102 96-97 -1.151451501231854 1102 98-99 -1.179749920214281 1102 100 -1.0947083879036938 1103 1 -0.33198151536119624 1103 2 -0.2734499598386648 1103 3 -0.33029667990872724 1103 4 -0.16793906352906163 1103 5 -0.16960001309696082 1103 6 -0.3225460539566356 1103 7 -0.3118628950286606 1103 8 -0.3145840108016458 1103 9 -0.47678150046938583 1103 10-11 -0.4785189907044938 1103 12-13 -0.5392281345258638 1103 14-15 -0.6813023071255913 1103 16-17 -0.7830339882474604 1103 18-19 -0.6540661618675188 1103 20-21 -0.6725879764586793 1103 22-23 -0.7940938684346008 1103 24-25 -0.7632163529809688 1103 26-27 -0.8491724167336372 1103 28-29 -0.8107002275961364 1103 30-31 -0.7240501987016046 1103 32-33 -0.7449334425537941 1103 34-35 -0.7702008791575139 1103 36-37 -0.7574604051911891 1103 38-39 -0.7278824644324686 1103 40-41 -0.8237873943603802 1103 42-43 -0.8387424984896299 1103 44-45 -0.8159091332774864 1103 46-47 -0.827664159854038 1103 48-49 -0.8595162914934207 1103 50-51 -0.8853508881021313 1103 52-53 -0.9165818777136323 1103 54-55 -0.9752172781778867 1103 56-57 -0.9682474116557884 1103 58-59 -1.0911523634811786 1103 60-61 -1.040872533058483 1103 62-63 -1.0630338754331845 1103 64-65 -0.9884742041789352 1103 66-67 -1.042690835170923 1103 68-69 -1.1084853527501721 1103 70-71 -1.0492091460739772 1103 72-73 -1.0253560290935937 1103 74-75 -1.0295123482280673 1103 76-77 -0.9558719982774235 1103 78-79 -0.8260321901431453 1103 80-81 -0.768162792781311 1103 82-83 -0.7991457698509912 1103 84-85 -0.8386116870739677 1103 86-87 -0.9058864034214231 1103 88-89 -0.8893188061901576 1103 90-91 -0.9530364851174227 1103 92-93 -0.9581776916922422 1103 94-95 -1.0297799971605883 1103 96-97 -1.1649241343311267 1103 98-99 -1.2285676710094329 1103 100 -1.2301341077963066 1104 1 -0.39604433149606066 1104 2 -0.3294124832360268 1104 3 -0.3855188794095419 1104 4 -0.21256675261195568 1104 5 -0.24366826165628908 1104 6 -0.3257038608990186 1104 7 -0.2974772033813693 1104 8 -0.3541637467238985 1104 9 -0.522995247701509 1104 10-11 -0.5012545906892356 1104 12-13 -0.5392269658334357 1104 14-15 -0.6289169802243606 1104 16-17 -0.689771135238928 1104 18-19 -0.7028609805808017 1104 20-21 -0.7021608832264974 1104 22-23 -0.7378785084069293 1104 24-25 -0.828886406252245 1104 26-27 -0.8327598133492558 1104 28-29 -0.7770731267931055 1104 30-31 -0.8097618448812867 1104 32-33 -0.7498345110294125 1104 34-35 -0.7906015171837737 1104 36-37 -0.7307265943890471 1104 38-39 -0.8519073545026998 1104 40-41 -0.8352922876755784 1104 42-43 -0.7603022570685596 1104 44-45 -0.8129553629303388 1104 46-47 -0.8373624827119883 1104 48-49 -0.8404816838881715 1104 50-51 -0.8039533417519351 1104 52-53 -0.8217637801294089 1104 54-55 -0.7040128790599667 1104 56-57 -0.7513968450594533 1104 58-59 -0.8384688603843458 1104 60-61 -0.8593156511732047 1104 62-63 -0.8664135458060542 1104 64-65 -0.8548509002010647 1104 66-67 -0.868493181004574 1104 68-69 -0.8456974204287491 1104 70-71 -0.8618232824293699 1104 72-73 -0.853994762947039 1104 74-75 -0.8469062085793624 1104 76-77 -0.8116131941325477 1104 78-79 -0.7352661772468245 1104 80-81 -0.8242417578511478 1104 82-83 -0.8123579517763808 1104 84-85 -0.7235534043825496 1104 86-87 -0.679870038648918 1104 88-89 -0.7481029350733337 1104 90-91 -0.7550998657592345 1104 92-93 -0.908414140974326 1104 94-95 -0.9815279363140093 1104 96-97 -1.0508179083987095 1104 98-99 -0.9235775139651032 1104 100 -0.9802546683969702 1105 1 -0.40279095875873594 1105 2 -0.37521270776501225 1105 3 -0.4317083028802742 1105 4 -0.2662516174988312 1105 5 -0.27735721447677264 1105 6 -0.28329654441996155 1105 7 -0.3202228655228794 1105 8 -0.3334220941383208 1105 9 -0.5052000577854869 1105 10-11 -0.4791707204304103 1105 12-13 -0.5093114754081896 1105 14-15 -0.7006340890717411 1105 16-17 -0.7087264299966094 1105 18-19 -0.6165998184857813 1105 20-21 -0.7159757167392513 1105 22-23 -0.7611101889702567 1105 24-25 -0.7126671070562338 1105 26-27 -0.7699308864548726 1105 28-29 -0.7808577311126399 1105 30-31 -0.8021369266207401 1105 32-33 -0.7431011388146089 1105 34-35 -0.6919760848578989 1105 36-37 -0.8135685304405413 1105 38-39 -0.8479058668636981 1105 40-41 -0.8548146679594453 1105 42-43 -0.8111395918895283 1105 44-45 -0.7881593946164358 1105 46-47 -0.9465252938886479 1105 48-49 -0.8723110879642704 1105 50-51 -0.9248991220746774 1105 52-53 -0.9163575647798297 1105 54-55 -0.9232774966883284 1105 56-57 -0.980440998584406 1105 58-59 -0.9937156309552151 1105 60-61 -1.0942558036715724 1105 62-63 -1.1031671425344811 1105 64-65 -1.0697484598122813 1105 66-67 -1.0258175362521484 1105 68-69 -1.0460042459715737 1105 70-71 -1.0663477105220807 1105 72-73 -1.1154911156957539 1105 74-75 -1.043598824403503 1105 76-77 -0.9958268547464613 1105 78-79 -0.8886011472904158 1105 80-81 -0.8507856635643414 1105 82-83 -0.8238856171744047 1105 84-85 -0.7340170160104122 1105 86-87 -0.810646261936153 1105 88-89 -0.8566287355437545 1105 90-91 -0.8799647219016222 1105 92-93 -0.9301403170428557 1105 94-95 -0.8718698858126643 1105 96-97 -0.9551051287652221 1105 98-99 -0.9683982096668551 1105 100 -1.0546300866993867 1106 1 -0.3680854144230068 1106 2 -0.33387708324936227 1106 3 -0.38615982303481644 1106 4 -0.21907902495361498 1106 5 -0.2595399254937263 1106 6 -0.3339874862503791 1106 7 -0.3471333932030731 1106 8 -0.379044173287717 1106 9 -0.4999562620967879 1106 10-11 -0.4626333739981945 1106 12-13 -0.526165864995825 1106 14-15 -0.6906525473462608 1106 16-17 -0.7052975298810225 1106 18-19 -0.6986670764681904 1106 20-21 -0.717012845765872 1106 22-23 -0.6397620816825977 1106 24-25 -0.753843190010052 1106 26-27 -0.7307885257806959 1106 28-29 -0.7418488049908873 1106 30-31 -0.7145757820106056 1106 32-33 -0.6672552453509226 1106 34-35 -0.7124370007853074 1106 36-37 -0.7680277176667616 1106 38-39 -0.7901072401519826 1106 40-41 -0.7835858185909288 1106 42-43 -0.7262854736864384 1106 44-45 -0.8206320332557766 1106 46-47 -0.8597843769618905 1106 48-49 -0.830536618143995 1106 50-51 -0.8876174072727849 1106 52-53 -0.881570719686998 1106 54-55 -0.9044853091707807 1106 56-57 -0.9435547052686033 1106 58-59 -0.9803404874411612 1106 60-61 -1.0264721970856385 1106 62-63 -0.8987773829917245 1106 64-65 -0.9195091875812622 1106 66-67 -0.9559130044741408 1106 68-69 -0.9505599993352902 1106 70-71 -0.828601180932111 1106 72-73 -0.9240405997098478 1106 74-75 -0.9535052540396123 1106 76-77 -0.981621226405295 1106 78-79 -0.799396418598171 1106 80-81 -0.7397270873659103 1106 82-83 -0.8010194755359699 1106 84-85 -0.8221314160546065 1106 86-87 -0.8475374374231599 1106 88-89 -0.8218576600931193 1106 90-91 -0.8679228810067698 1106 92-93 -0.7496910403949677 1106 94-95 -0.8348400841686328 1106 96-97 -0.9595943310299084 1106 98-99 -1.0158666778317773 1106 100 -1.0262693249614898 1107 1 -0.4111776958467033 1107 2 -0.319000034655609 1107 3 -0.406386128597763 1107 4 -0.21958662471417512 1107 5 -0.24748174282500912 1107 6 -0.3065065398972493 1107 7 -0.3429239606385437 1107 8 -0.37478686695013863 1107 9 -0.5386938058270516 1107 10-11 -0.49823470341192433 1107 12-13 -0.541281471164659 1107 14-15 -0.7131630212170137 1107 16-17 -0.6589028459439774 1107 18-19 -0.6793667667773065 1107 20-21 -0.693713939453886 1107 22-23 -0.724383131493596 1107 24-25 -0.7675231570135068 1107 26-27 -0.7657789077827886 1107 28-29 -0.7708589682370146 1107 30-31 -0.7838666355789314 1107 32-33 -0.7886490012791754 1107 34-35 -0.8198334527991378 1107 36-37 -0.7956661078864684 1107 38-39 -0.7937000539274521 1107 40-41 -0.8095594605390417 1107 42-43 -0.8226776663503941 1107 44-45 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1.0876038347049999 2311 46-47 1.1414714072552599 2311 48-49 1.1389716204866431 2311 50-51 1.1337952889356586 2311 52-53 1.1294390454057677 2311 54-55 1.1148664467081133 2311 56-57 1.1527722034530683 2311 58-59 1.1504919579659472 2311 60-61 1.175618927687509 2311 62-63 1.2029511031960212 2311 64-65 1.2185675487638576 2311 66-67 1.2421840080093816 2311 68-69 1.207649629330895 2311 70-71 1.2231945624739282 2311 72-73 1.2340012859324716 2311 74-75 1.1564519593739107 2311 76-77 1.0235491062822817 2311 78-79 0.940268947714344 2311 80-81 0.9985006935748189 2311 82-83 1.019141407609574 2311 84-85 1.0138633646616526 2311 86-87 1.0878502492635427 2311 88-89 1.1018490966606187 2311 90-91 1.1397839698553724 2311 92-93 1.1886578026208667 2311 94-95 1.2080173528318952 2311 96-97 1.2854156551380562 2311 98-99 1.3064542011602676 2311 100 1.3586886341907523 2312 1 0.1315508153728544 2312 2 0.1521189052266294 2312 3 0.3410115380933121 2312 4 0.25178752762881373 2312 5 0.32780085784796853 2312 6 0.4095032567810719 2312 7 0.4745114006429745 2312 8 0.2830265132881493 2312 9 0.41578267148695147 2312 10-11 0.27935688124720315 2312 12-13 0.4520355150371884 2312 14-15 0.8085425448072527 2312 16-17 0.8319290814248745 2312 18-19 0.8809637913621984 2312 20-21 0.8825851608496862 2312 22-23 0.5262030596271856 2312 24-25 0.5339592503650366 2312 26-27 0.8350349082017701 2312 28-29 0.9546145342567414 2312 30-31 0.8808324058517698 2312 32-33 0.7394550021487944 2312 34-35 0.9181856997359858 2312 36-37 0.9953496884597897 2312 38-39 1.020089104328207 2312 40-41 1.0129625678489944 2312 42-43 0.8777340812531804 2312 44-45 0.8495688359026303 2312 46-47 1.0185252651156702 2312 48-49 1.0900412844279828 2312 50-51 1.083599114752353 2312 52-53 1.1107894498738418 2312 54-55 1.0938174220522754 2312 56-57 1.130881981073692 2312 58-59 1.1711790272205675 2312 60-61 1.2095183650418662 2312 62-63 1.1743255983824596 2312 64-65 1.266193922860836 2312 66-67 1.2404879540996347 2312 68-69 1.2461597937937583 2312 70-71 1.0792219747555407 2312 72-73 1.1911260551121856 2312 74-75 1.210352261477695 2312 76-77 1.145853357166743 2312 78-79 1.0331546703265744 2312 80-81 0.9374516562454289 2312 82-83 0.9695876011679516 2312 84-85 0.7806976753467012 2312 86-87 0.9994586250778958 2312 88-89 0.6146969034197518 2312 90-91 0.9779059112445481 2312 92-93 1.1580731324402223 2312 94-95 1.1971500993004724 2312 96-97 1.281927071070772 2312 98-99 1.3133728105703 2312 100 1.4145102430851502 2313 1 -0.3648870494558949 2313 2 -0.05838112993758671 2313 3 0.23754551844823624 2313 4 0.11340344060909757 2313 5 0.23936402450291183 2313 6 0.342522456443497 2313 7 0.11068717484181434 2313 8 0.060384274030823804 2313 9 0.25594137931919647 2313 10-11 0.3181453567611854 2313 12-13 0.5119442052873282 2313 14-15 0.5804275812608211 2313 16-17 0.7232945079668269 2313 18-19 0.5963838865400035 2313 20-21 0.7868318261097826 2313 22-23 0.8463087867059826 2313 24-25 0.7347697855644171 2313 26-27 0.9081794096903906 2313 28-29 0.78637309674356 2313 30-31 0.7716856069676439 2313 32-33 0.9432697336113876 2313 34-35 0.9352561688266405 2313 36-37 0.7484822341146682 2313 38-39 0.5529289664844725 2313 40-41 0.7060381474725048 2313 42-43 0.6811737277355903 2313 44-45 0.9213712364465039 2313 46-47 0.9533454587532972 2313 48-49 1.0479766760401503 2313 50-51 0.9455502599336825 2313 52-53 0.8709190886201199 2313 54-55 1.007373813734766 2313 56-57 1.0488368302192086 2313 58-59 0.9899760593606572 2313 60-61 1.0021913143819532 2313 62-63 1.0477994713693022 2313 64-65 1.1381299474289008 2313 66-67 1.1419279287813993 2313 68-69 1.1295323938359658 2313 70-71 1.070386965261207 2313 72-73 1.161132736313398 2313 74-75 0.7765225266158922 2313 76-77 0.9407123486570015 2313 78-79 0.8154406960667799 2313 80-81 0.8951110087549736 2313 82-83 0.8844274867670308 2313 84-85 0.9300002517116539 2313 86-87 1.012142944181445 2313 88-89 1.0362461216019945 2313 90-91 1.1146484154056466 2313 92-93 0.8137949365989812 2313 94-95 0.843172440299611 2313 96-97 1.1350391042657932 2313 98-99 1.161356728801774 2313 100 1.2501351112193397 2314 1 -0.39601985379074023 2314 2 -0.20838904066302533 2314 3 0.08071138307128223 2314 4 -0.1904394011852446 2314 5 0.16868993060818127 2314 6 0.2103599874656652 2314 7 0.10330048202933284 2314 8 0.1529827798853347 2314 9 0.23755527509548813 2314 10-11 0.36289597450991806 2314 12-13 0.48811904425306096 2314 14-15 0.5583531542302396 2314 16-17 0.7614696479635725 2314 18-19 0.7809962167240627 2314 20-21 0.43953177017652934 2314 22-23 0.6447652039980909 2314 24-25 0.8574243998270461 2314 26-27 0.795653478486372 2314 28-29 0.7535368880251312 2314 30-31 0.7131782376745761 2314 32-33 0.788461841055593 2314 34-35 0.8354087245683814 2314 36-37 0.7614010646823246 2314 38-39 0.7842752160499629 2314 40-41 0.8382129252311614 2314 42-43 0.6535671629498196 2314 44-45 0.6670579182109151 2314 46-47 0.7772856717616108 2314 48-49 0.6602259050812478 2314 50-51 0.8106717174781082 2314 52-53 0.885982255089111 2314 54-55 0.9631707490696826 2314 56-57 0.8030776719665838 2314 58-59 0.7966827377452077 2314 60-61 0.8440349907482059 2314 62-63 0.6244519757201985 2314 64-65 0.7573334259153839 2314 66-67 0.8328811192289365 2314 68-69 0.8836361837086102 2314 70-71 0.9520718169726337 2314 72-73 0.9865174169914823 2314 74-75 1.0182350312208541 2314 76-77 0.7842023124293434 2314 78-79 0.7668142462964198 2314 80-81 0.7250037539460834 2314 82-83 0.7264445158065129 2314 84-85 0.613234989689559 2314 86-87 0.8177088516879358 2314 88-89 0.8228204560520744 2314 90-91 0.9185455544176904 2314 92-93 1.00809524929398 2314 94-95 1.0392983309298813 2314 96-97 1.0343948420824098 2314 98-99 1.099167879190631 2314 100 1.157413613794077 2315 1 -0.030310112023471447 2315 2 0.13676516760026658 2315 3 0.18382305385605235 2315 4 -0.02339238384842446 2315 5 0.2655676164344527 2315 6 0.3820744367143618 2315 7 0.443863008978596 2315 8 0.39145607857156506 2315 9 0.5875785259949282 2315 10-11 0.5765929866393762 2315 12-13 0.6505062540851227 2315 14-15 0.7432588489377849 2315 16-17 0.7523663232882072 2315 18-19 0.8374926263978395 2315 20-21 0.8279711960722054 2315 22-23 0.9128552201963274 2315 24-25 0.8263259944650798 2315 26-27 0.9098305580752992 2315 28-29 0.9310578448599358 2315 30-31 0.9098641275212103 2315 32-33 0.9552023992448611 2315 34-35 0.9531816563078479 2315 36-37 1.0248175318538486 2315 38-39 0.9887444236371437 2315 40-41 0.9576447310583873 2315 42-43 0.9912700730724211 2315 44-45 0.876371897852799 2315 46-47 0.9145840993914405 2315 48-49 1.0276694775795079 2315 50-51 0.9152002332297684 2315 52-53 0.9636597465474779 2315 54-55 0.8811423888354071 2315 56-57 1.0014990488556563 2315 58-59 1.016679093176002 2315 60-61 1.0979001132127522 2315 62-63 1.09069762120901 2315 64-65 1.0810250087634117 2315 66-67 1.1297679812160624 2315 68-69 1.0450197249916116 2315 70-71 0.6668939751482057 2315 72-73 0.8686843718954336 2315 74-75 1.040190946737681 2315 76-77 1.0720139117611325 2315 78-79 0.9514750218883421 2315 80-81 0.9222676012130222 2315 82-83 0.9142287283461243 2315 84-85 0.8734437776657344 2315 86-87 0.8488551386197507 2315 88-89 0.9792244997346806 2315 90-91 1.0102846707853956 2315 92-93 1.036280762254023 2315 94-95 1.1207444512748737 2315 96-97 1.137380220340848 2315 98-99 1.0719291045702946 2315 100 0.9661064854380719 2316 1 0.4922882248881919 2316 2 0.5026914837474408 2316 3 0.47809285534129486 2316 4 0.31645175360242916 2316 5 0.40325127911881964 2316 6 0.41705406221795016 2316 7 0.4756358815359718 2316 8 0.4589423660218941 2316 9 0.5327569657635323 2316 10-11 0.4737923000473856 2316 12-13 0.6105409019854591 2316 14-15 0.7583058178848177 2316 16-17 0.7756177605218113 2316 18-19 0.8429503698454752 2316 20-21 0.8682847322198768 2316 22-23 0.8790392495010764 2316 24-25 0.9515314710704033 2316 26-27 0.9876898715589562 2316 28-29 0.9386907534601576 2316 30-31 0.814793378210517 2316 32-33 0.7819336198370479 2316 34-35 0.6886391914194476 2316 36-37 0.7015296379604479 2316 38-39 0.6959848270468854 2316 40-41 0.8173197781721697 2316 42-43 0.9486073179136767 2316 44-45 0.8490846213630334 2316 46-47 0.8680207516561964 2316 48-49 0.6142386710321759 2316 50-51 0.7220002046189791 2316 52-53 0.8766114469977211 2316 54-55 0.9152369415951185 2316 56-57 0.9147724107021631 2316 58-59 0.8559656037410122 2316 60-61 0.9367887386800291 2316 62-63 0.944505108281426 2316 64-65 0.9375548329068479 2316 66-67 0.9474481387789808 2316 68-69 0.9424124421772362 2316 70-71 0.7898263228645988 2316 72-73 0.8697819502602044 2316 74-75 0.9170528105320983 2316 76-77 0.7466421613387766 2316 78-79 0.6736689817471557 2316 80-81 0.7104443130519655 2316 82-83 0.660920837961946 2316 84-85 0.7624362493688253 2316 86-87 0.7150639177877913 2316 88-89 0.6684639872768834 2316 90-91 0.7379932359474424 2316 92-93 0.7293120014628727 2316 94-95 0.6867479080501511 2316 96-97 0.803813283009557 2316 98-99 0.9207698404666118 2316 100 0.6387567138618131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 12.0 12 44.0 13 260.0 14 1041.0 15 3145.0 16 7386.0 17 15057.0 18 25211.0 19 36691.0 20 52085.0 21 70946.0 22 86934.0 23 90380.0 24 94460.0 25 104320.0 26 124417.0 27 153946.0 28 190075.0 29 232511.0 30 284594.0 31 355846.0 32 445582.0 33 579262.0 34 802581.0 35 1121298.0 36 1718377.0 37 2657613.0 38 2460212.0 39 406248.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.83205973284519 20.351424254986224 13.802852686794326 24.01366332537426 2 15.287840432150688 20.864897054903363 40.26438653507458 23.582875977871367 3 17.94430418262746 26.50185176675024 30.598320031859842 24.955524018762457 4 12.24697410330522 17.57885188805688 39.04026051337877 31.133913495259126 5 12.902308379212169 37.99956584692152 35.32083640225875 13.777289371607559 6 29.99722950689018 38.325795468964955 18.18138165926961 13.495593364875264 7 26.403622801711208 32.77029022741846 23.085039607067788 17.741047363802544 8 25.46059183632554 35.734245327376726 20.465924516443028 18.339238319854704 9 26.150946263693804 15.509110997014691 20.705345793146453 37.63459694614505 10-11 24.51183196459781 26.341382056761965 28.84257146790269 20.304214510737538 12-13 25.250629942067853 24.12665125428621 28.604992558511533 22.0177262451344 14-15 22.595670125054237 25.608537247513137 26.236031919591973 25.559760707840656 16-17 21.88023528754642 28.29654471382893 27.092690787279995 22.73052921134466 18-19 21.616964590906367 27.724795837033305 29.028825089081906 21.629414482978422 20-21 22.62589729067934 26.925157776013194 28.683583700109512 21.765361233197954 22-23 22.532449234201664 26.823751474402663 28.434135592365926 22.209663699029747 24-25 22.207902677884505 26.943655725904094 28.750104677377102 22.098336918834296 26-27 22.197563071419406 27.087327055583536 28.47510660582802 22.240003267169033 28-29 21.87929709210738 27.479055385478386 28.522017509457235 22.119630012956996 30-31 22.52589076752146 27.072646453152444 28.439715940492 21.961746838834095 32-33 22.44345305372633 27.26461634421072 28.099412235798315 22.192518366264636 34-35 22.50932556688273 27.156260372171033 28.505692717846742 21.828721343099495 36-37 22.371230639710216 27.001396481948277 28.480923756179088 22.14644912216242 38-39 22.273342318799987 26.92902765923367 28.696872699461558 22.100757322504784 40-41 22.23373433350094 26.575548003379545 28.96405395505995 22.22666370805957 42-43 22.083919054116556 26.761568147042087 28.565762796159927 22.588750002681433 44-45 21.92250406640646 27.11356803042521 28.598539715958427 22.365388187209902 46-47 22.04995181286882 26.818218931512718 28.269712113711314 22.862117141907152 48-49 22.200576107606974 26.802064825637185 28.296900863847878 22.700458202907964 50-51 21.996545525161284 26.919415582663532 28.10548137346936 22.978557518705824 52-53 21.730068969119547 27.1463664228572 28.07336885451552 23.050195753507737 54-55 21.684849907555602 27.0055545023814 28.146076791041835 23.16351879902116 56-57 22.02914718428329 26.849953398217334 27.857540332622825 23.263359084876548 58-59 21.656833759055843 26.994212057872407 28.12583838936829 23.22311579370346 60-61 21.826920510523156 27.194990128412115 27.736239191303287 23.241850169761445 62-63 21.782486468041036 26.942668535185526 27.670280373122385 23.604564623651047 64-65 21.378462068112718 26.917630764529672 27.717802042954858 23.986105124402755 66-67 21.61783040978874 26.883268834174086 27.528469531318034 23.970431224719142 68-69 21.469167090904154 26.997345444168186 27.519667297650273 24.013820167277384 70-71 21.481813705313478 26.762451135570604 27.486162859818574 24.26957229929734 72-73 21.611236362180698 26.992208021598486 27.352287307382632 24.044268308838184 74-75 21.704217613103026 27.175484838086355 27.090287326924887 24.03001022188573 76-77 21.626934086165228 27.0774167597308 27.017753104457736 24.277896049646237 78-79 21.486476573594977 27.139941551673765 27.035915512075544 24.337666362655717 80-81 21.41791197165595 27.395301664988015 26.935306982487933 24.251479380868098 82-83 21.69197661909036 26.992347924889803 27.186888990195452 24.128786465824383 84-85 21.825287258407887 27.137744619406906 26.65385670357115 24.38311141861406 86-87 21.38916724585092 27.340982967855687 26.873131408515576 24.396718377777816 88-89 21.57865052501267 26.93413553989946 26.804733625229932 24.68248030985794 90-91 21.592629575003507 26.823309009905234 26.84552275782587 24.73853865726539 92-93 21.64066440944008 26.959352384622825 26.73053637224721 24.669446833689882 94-95 21.23905802137557 26.791173362625532 26.977002692223245 24.99276592377565 96-97 21.34850140681009 26.704928292763363 26.8837957086424 25.062774591784155 98-99 21.17401980646421 26.915412141184643 26.773514172714624 25.13705387963652 100 21.287469456159343 26.738625428764927 26.735291610487565 25.23861350458817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 832.0 1 600.0 2 554.0 3 802.0 4 735.5 5 684.5 6 993.5 7 1873.0 8 3108.5 9 3936.0 10 4024.0 11 4105.5 12 4709.0 13 5563.0 14 6536.5 15 7717.5 16 8996.5 17 10291.5 18 11705.0 19 13270.0 20 15019.0 21 17171.5 22 20456.0 23 25191.0 24 31345.5 25 39266.5 26 49743.5 27 62615.5 28 78872.0 29 100644.0 30 125632.5 31 150895.5 32 183021.0 33 212488.5 34 238671.5 35 268033.5 36 298083.5 37 336639.5 38 359669.0 39 361797.5 40 364888.5 41 372313.5 42 385990.5 43 404629.5 44 428469.0 45 462944.5 46 490988.0 47 509818.5 48 528205.0 49 527674.0 50 511569.5 51 502042.5 52 489465.0 53 465463.0 54 438961.5 55 394976.0 56 347970.5 57 299461.5 58 249933.5 59 214239.5 60 173420.0 61 132649.0 62 100970.0 63 75956.0 64 54269.5 65 35870.5 66 24455.5 67 18284.5 68 13824.0 69 10075.0 70 7493.5 71 5718.5 72 4130.5 73 2682.5 74 1799.0 75 1161.5 76 751.5 77 460.5 78 260.5 79 163.0 80 95.0 81 56.0 82 42.0 83 34.5 84 24.5 85 17.0 86 11.5 87 7.5 88 7.0 89 5.0 90 4.0 91 2.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050434851051355846 2 0.0 3 0.0 4 0.01947925459385754 5 0.0036136863668401542 6 8.250425495068845E-6 7 1.897597863865834E-4 8 3.300170198027538E-4 9 0.0019305995658461096 10-11 7.714147837889371E-4 12-13 2.3101191386192763E-4 14-15 4.9502552970413065E-5 16-17 1.113807441834294E-4 18-19 0.0 20-21 2.062606373767211E-5 22-23 4.125212747534422E-6 24-25 9.900510594082613E-5 26-27 1.650085099013769E-5 28-29 8.951711662149697E-4 30-31 8.126669112642811E-4 32-33 0.013996846852384295 34-35 0.023307452023569486 36-37 0.03060907858670541 38-39 0.02062606373767211 40-41 1.526328716587736E-4 42-43 0.001592332120548287 44-45 0.009603495276260135 46-47 0.013922593022928675 48-49 0.005189517636398303 50-51 0.010181025060914953 52-53 0.015696434504368476 54-55 0.009401359851630948 56-57 0.0028257707320610793 58-59 0.008205048154845965 60-61 0.0016212086097810278 62-63 9.364232936903139E-4 64-65 0.01902960640437629 66-67 0.04489881554416465 68-69 0.05524484911498098 70-71 0.048005100743058075 72-73 0.040608594286728854 74-75 0.028455717532492444 76-77 0.028835237105265612 78-79 0.01242514079557368 80-81 0.0018934726511183 82-83 0.027647175833975697 84-85 0.04783596702040916 86-87 0.04824023786966754 88-89 0.05911429867216827 90-91 0.03478791909995778 92-93 0.026479740626423453 94-95 0.007128367627739482 96-97 0.003601310728597551 98-99 0.0224824094740626 100 0.019388499913411784 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2120587E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.62972376764938 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67395537132178 43.33527883894397 2 15.802271367940183 18.84570153143416 3 5.467646257761458 9.781027080286126 4 2.3887937343822236 5.6977244207641435 5 1.1910882996520737 3.551213314556617 6 0.6943241637142734 2.484141485249177 7 0.44838284895938485 1.8715861797919842 8 0.2942789113737139 1.4038216156687309 9 0.1977514880838781 1.0612679948174932 >10 0.796271792021008 7.894147354295479 >50 0.03187684700632256 1.2940548733251627 >100 0.012177873734266325 1.2579643075920133 >500 7.271336961826009E-4 0.27948947112489186 >1k 3.812847290951361E-4 0.4356414536605363 >5k 1.8156406040046753E-5 0.09217779813315721 >10k+ 5.4469218120140266E-5 0.7147622803562258 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28321 0.23366030044584477 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23658 0.19518856636233872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14040 0.11583597395076657 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.250425495068845E-6 2 0.0 0.0 0.0 0.0 8.250425495068845E-6 3 0.0 0.0 0.0 0.0 8.250425495068845E-6 4 0.0 0.0 0.0 8.250425495068845E-6 8.250425495068845E-6 5 0.0 0.0 0.0 1.650085099013769E-5 8.250425495068845E-6 6 0.0 0.0 0.0 2.4751276485206533E-5 1.650085099013769E-5 7 0.0 0.0 0.0 2.4751276485206533E-5 1.650085099013769E-5 8 0.0 0.0 0.0 3.300170198027538E-5 2.4751276485206533E-5 9 0.0 0.0 0.0 8.250425495068844E-5 2.4751276485206533E-5 10-11 2.4751276485206533E-5 0.0 0.0 1.2375638242603267E-4 3.300170198027538E-5 12-13 3.300170198027538E-5 0.0 0.0 1.5675808440630804E-4 1.320068079211015E-4 14-15 4.537734022287865E-5 0.0 0.0 1.815093608915146E-4 1.815093608915146E-4 16-17 6.187819121301633E-5 0.0 0.0 2.3101191386192763E-4 2.3101191386192763E-4 18-19 6.600340396055076E-5 0.0 0.0 3.2589180705521934E-4 2.4751276485206533E-4 20-21 6.600340396055076E-5 0.0 0.0 5.156515934418028E-4 3.093909560650817E-4 22-23 8.250425495068844E-5 0.0 0.0 0.0010395536123786744 3.3826744529782266E-4 24-25 9.900510594082614E-5 0.0 0.0 0.0025081293505009286 3.5064308354042586E-4 26-27 1.0725553143589498E-4 0.0 0.0 0.00539165306102749 3.630187217830292E-4 28-29 1.1963116967849826E-4 0.0 0.0 0.017198011944471008 3.795195727731668E-4 30-31 1.3613202066863593E-4 0.0 0.0 0.059543320797911845 3.795195727731668E-4 32-33 1.4438244616370478E-4 4.125212747534422E-6 0.0 0.15403956920568285 3.877699982682357E-4 34-35 1.6088329715384245E-4 8.250425495068845E-6 0.0 0.29641303676133834 4.3727255123864875E-4 36-37 1.650085099013769E-4 8.250425495068845E-6 0.0 0.5225283230919426 4.3727255123864875E-4 38-39 1.650085099013769E-4 1.650085099013769E-5 0.0 0.849987710991225 4.743994659664586E-4 40-41 1.9801021188165226E-4 1.650085099013769E-5 0.0 1.1960765596583731 4.8677510420906184E-4 42-43 1.9801021188165226E-4 1.650085099013769E-5 0.0 1.540408067695071 4.8677510420906184E-4 44-45 2.0213542462918667E-4 1.650085099013769E-5 0.0 1.9445056580180482 4.950255297041307E-4 46-47 2.062606373767211E-4 1.650085099013769E-5 0.0 2.4195899093005973 5.280272316844061E-4 48-49 2.062606373767211E-4 1.650085099013769E-5 0.0 2.9152135948531206 5.610289336646815E-4 50-51 2.1451106287178996E-4 1.650085099013769E-5 0.0 3.42115031227448 5.775297846548191E-4 52-53 2.1451106287178996E-4 1.650085099013769E-5 0.0 3.9055369183027193 6.600340396055076E-4 54-55 2.1451106287178996E-4 1.650085099013769E-5 0.0 4.417884216333747 6.765348905956453E-4 56-57 2.1451106287178996E-4 1.650085099013769E-5 0.0 5.002888886487098 6.847853160907141E-4 58-59 2.1451106287178996E-4 1.650085099013769E-5 0.0 5.646983104036133 6.889105288382485E-4 60-61 2.1863627561932437E-4 1.650085099013769E-5 0.0 6.2961513332646355 7.095365925759206E-4 62-63 2.3101191386192763E-4 1.650085099013769E-5 0.0 6.9353984258353165 7.92040847526609E-4 64-65 2.3101191386192763E-4 1.650085099013769E-5 0.0 7.5566183386992725 8.291677622544189E-4 66-67 2.3101191386192763E-4 1.650085099013769E-5 0.0 8.23721243863849 8.374181877494878E-4 68-69 2.4751276485206533E-4 1.650085099013769E-5 0.0 8.970992906531672 8.49793825992091E-4 70-71 2.6401361584220303E-4 1.650085099013769E-5 0.0 9.732998080043483 8.745451024772975E-4 72-73 2.8051446683234073E-4 1.650085099013769E-5 0.0 10.476167532150052 9.322980809427794E-4 74-75 2.8051446683234073E-4 1.650085099013769E-5 0.0 11.19050999757685 9.77675421165658E-4 76-77 2.8051446683234073E-4 2.062606373767211E-5 0.0 11.944545260060424 9.900510594082613E-4 78-79 2.8051446683234073E-4 2.4751276485206533E-5 0.0 12.739906903848798 0.0010106771231459333 80-81 2.8051446683234073E-4 2.4751276485206533E-5 0.0 13.560778863267927 0.00103542839963114 82-83 2.8051446683234073E-4 2.4751276485206533E-5 0.0 14.38035138067158 0.0010973065908441563 84-85 2.8051446683234073E-4 2.4751276485206533E-5 0.0 15.159566941766105 0.0010973065908441563 86-87 2.8051446683234073E-4 2.4751276485206533E-5 0.0 15.930676459811723 0.0011715604202997758 88 2.8051446683234073E-4 2.4751276485206533E-5 0.0 16.52723585087092 0.0012375638242603266 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 18645 0.0 49.427605 1 GTATCAA 32670 0.0 39.17553 1 ATCAACG 38940 0.0 31.94564 3 CAACGCA 39080 0.0 31.801113 5 TATCAAC 41025 0.0 30.825476 2 TCAACGC 41795 0.0 29.83128 4 AACGCAG 42525 0.0 29.765537 6 GTACTAG 5000 0.0 27.553293 1 ACGCAGA 46450 0.0 26.633337 7 CGCAGAG 47110 0.0 26.150501 8 TACCTGG 8420 0.0 24.557562 2 GTACATG 39585 0.0 23.61357 1 TACATGG 39740 0.0 22.98863 2 GCAGAGT 52545 0.0 22.926897 9 ACATGGG 38915 0.0 22.425829 3 CTAACGC 2060 0.0 21.67255 3 GTGGTAT 8590 0.0 21.6212 1 TCTAACG 2080 0.0 21.463717 2 CTGTGCG 6305 0.0 20.865635 9 TAACGCC 2100 0.0 20.812424 4 >>END_MODULE