FastQCFastQC Report
Mon 6 Jun 2016
ERR1211413_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211413_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6227014
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190690.30623024133236254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150130.24109468840121445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94620.15195083871659837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95300.051.7195171
TACCTGG66750.050.2829132
GTATCAA177050.042.6631851
ACCTGGG80000.040.958623
ATCAACG215300.034.434213
CAACGCA218600.033.8926165
TATCAAC222650.033.4265332
TCAACGC223250.033.1869434
GTACCTG104150.032.756311
AACGCAG233400.032.2878156
CCTGGGG74400.031.2776994
GTACATG208200.030.6219431
TAACGCA6750.029.9479544
TACATGG213200.029.1011662
ACGCAGA256400.029.0619147
CGCAGAG260450.028.5711448
CATGGGG164600.028.218224
ACATGGG213200.028.0921343
ATGGGGG132800.027.4702535
TGGGGGG150900.026.8860866
Produced by FastQC (version 0.11.5)