FastQCFastQC Report
Mon 6 Jun 2016
ERR1211413_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211413_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6227014
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175830.28236647613125654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149070.23939242789561738No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94620.15195083871659837No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95200.055.262411
TACCTGG63100.051.5327342
ACCTGGG79200.039.8710333
GTATCAA172000.039.6620941
CCTGGGG70800.032.5885544
ATCAACG212600.031.5630053
CAACGCA214950.031.235285
GTACCTG105350.031.105341
TATCAAC219650.030.8061712
TCAACGC222100.030.2555034
CTGGGGG49700.029.9677075
AACGCAG228350.029.7927916
GTACATG197700.028.1091371
TGGGGGG123500.027.6573836
TACATGG202900.026.8647232
ACGCAGA252300.026.6480877
ACATGGG196850.026.1869323
CGCAGAG256200.026.1324068
CATGGGG152900.025.8159854
ATGGGGG115600.025.6461495
Produced by FastQC (version 0.11.5)