FastQCFastQC Report
Mon 6 Jun 2016
ERR1211412_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211412_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514127
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157970.2864823389087702No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122400.2219753008953185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76000.1378278012095115No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59000.049.5383872
GGTATCA86950.049.4184761
ACCTGGG68850.041.5806473
GTATCAA153600.039.78441
ATCAACG184350.032.690593
CAACGCA185200.032.6158145
GTACCTG91900.032.196471
TATCAAC190950.031.59882
TCAACGC191550.031.4854974
CCTGGGG64300.031.435734
AACGCAG198200.030.9991536
GTACATG180000.029.002231
ACGCAGA219200.027.6004337
TACATGG185250.027.515192
CGCAGAG222550.027.0361278
ACATGGG183950.026.8584423
CATGGGG139250.026.4321584
ATGGGGG107700.026.2316025
TGGGGGG117550.026.153696
ATAACGC6200.025.0207393
Produced by FastQC (version 0.11.5)