FastQCFastQC Report
Mon 6 Jun 2016
ERR1211412_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211412_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5514127
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146920.26644290202238724No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122510.2221747885023323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76960.13956878396163164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG59600.049.5188372
GGTATCA85300.049.22661
ACCTGGG66150.043.6920473
GTATCAA148500.039.4538151
CCTGGGG60900.032.1792454
CAACGCA181950.031.3763585
ATCAACG182150.031.3217933
GTACCTG95800.031.0695951
TATCAAC189750.030.6616782
TAACGCA6300.030.584434
ATAACGC6250.030.0771773
TCAACGC190750.030.032834
AACGCAG195550.029.4817496
GTACATG179750.028.0952451
TACATGG184750.026.93822
CTGGGGG44850.026.5055245
ACATGGG179100.026.3972973
TGGGGGG103750.026.176186
ACGCAGA218800.026.112767
CGCAGAG224300.025.3467678
Produced by FastQC (version 0.11.5)