FastQCFastQC Report
Mon 6 Jun 2016
ERR1211411_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211411_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC110.10540436949022614No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG254.584781E-794.02
CCTGGGG156.709863E-494.04
ATCAACG200.002103039370.499994
GGTATCA200.002103039370.499991
GTACCTG353.3915749E-667.142861
ACCTGGG353.3915749E-667.142863
GTATCAA250.00509175456.3999982
TCAACGC250.00509175456.3999985
TATCAAC250.00509175456.3999983
CAACGCA250.00509175456.3999986
Produced by FastQC (version 0.11.5)