FastQCFastQC Report
Mon 6 Jun 2016
ERR1211411_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211411_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10436
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG360.34495975469528556TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG350.33537753928708314RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG230.22039095438865464RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.18206209275584515No Hit
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC190.18206209275584515No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT170.16289766193944039No Hit
GGTCACAAGCCTCGTGCTCTGCATTGTGAGCCATGTTGTAGGGGACGATT140.13415101571483326No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC130.1245688003066309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAGTA205.548415E-446.99999610-11
GTACATG459.682626E-441.777781
TACATGG459.682626E-441.777782
ACATGGG459.2061365E-431.3333343
GAGTACA350.00864159626.85714312-13
Produced by FastQC (version 0.11.5)