FastQCFastQC Report
Mon 6 Jun 2016
ERR1211410_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211410_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6684
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC300.4488330341113106No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG250.37402752842609216TruSeq Adapter, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.25433871932974267No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG170.25433871932974267RNA PCR Primer, Index 26 (100% over 29bp)
CTTCTTGGCCGCCTGGCGGCCATTGTGGCCAAGCAGGTACTTCTGGGCCG150.2244165170556553No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG140.20945541591861158RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG130.19449431478156792RNA PCR Primer, Index 33 (96% over 29bp)
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA130.19449431478156792No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT130.19449431478156792No Hit
GGTCACAAGCCTCGTGCTCTGCATTGTGAGCCATGTTGTAGGGGACGATT110.16457211250748055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.16457211250748055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.16457211250748055No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT100.14961101137043686No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG90.13464991023339318RNA PCR Primer, Index 46 (96% over 28bp)
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG90.13464991023339318No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG80.11968880909634949No Hit
CTGTAGGATCAGCTTGGTATTTCAAGGGATCCTCCTTGCCACGGTGGTCA70.10472770795930579No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG70.10472770795930579RNA PCR Primer, Index 17 (96% over 28bp)
GTATAAAATCAGGCAGTTTTTGATCACGTATCTCGTATGCCGTCTTCTGC70.10472770795930579TruSeq Adapter, Index 2 (96% over 25bp)
GGTGCCACACGACTACAACCCTCGTAATAGGGCTGCGTGCGTCCTAAATA70.10472770795930579No Hit
GTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGC70.10472770795930579No Hit
GGTGGCTGCTAATCTGGCTGAAGTCATGTGGTTCCTGTGACTGGGGAGTC70.10472770795930579No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG404.5344225E-429.37512-13
CTATCTC350.00853122726.85714124-25
TCTGCTT905.004271E-620.88888742-43
TGAAAAA757.717614E-418.848-49
TGCTTGA901.2160629E-418.27777744-45
CTTGAAA901.2160629E-418.27777746-47
GTCTTCT1051.8958925E-517.90476238-39
CCCACGA1350.00901344717.4074089
TGCCGTC951.8401892E-417.31578834-35
CCGTCTT951.8401892E-417.31578836-37
ATCTCGT1002.7235335E-416.44999926-27
CTCGTAT1002.7235335E-416.44999928-29
CACGAGA1154.143461E-516.34782610-11
CTTCTGC1205.9662565E-515.66666740-41
TATGCCG1053.9510045E-415.66666632-33
CGTATGC1105.628546E-414.95454530-31
AAAAAAA2705.594866E-48.70370452-53
Produced by FastQC (version 0.11.5)