FastQCFastQC Report
Mon 6 Jun 2016
ERR1211409_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211409_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523221
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG146350.049.7197232
ACCTGGG179650.039.9327133
GTATCAA192500.036.6886141
GTACATG447500.033.7965551
TACATGG456050.032.292752
GGTATCA118250.032.1139951
CAACGCA210150.032.0116655
ATCAACG219400.030.7479953
ACATGGG465850.030.496493
GTACCTG246950.030.1636091
TGGGGGG269650.029.7425636
TATCAAC235400.029.4715522
GTACTAG52700.029.2346271
CATGGGG371850.029.1281364
AACGCAG241300.028.8729176
CCTGGGG180950.028.73394
TCAACGC238000.028.54234
ATGGGGG277500.027.8338075
ACGCAGA268100.025.1802987
TAGTACT84150.023.742854
Produced by FastQC (version 0.11.5)