FastQCFastQC Report
Mon 6 Jun 2016
ERR1211409_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211409_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523221
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG147850.050.1861422
ACCTGGG176000.041.5995033
GTATCAA196150.035.2620541
GGTATCA124150.034.3513451
GTACTAG51700.032.3774531
GTACATG447050.030.9961131
TGGGGGG242300.030.8118066
CCTGGGG174050.030.7259334
GTACCTG246850.030.667331
CAACGCA218350.030.5565555
TACATGG456150.029.5148072
ATCAACG226000.029.2562753
ACATGGG460200.028.1018683
TATCAAC245250.027.7831822
TCAACGC241950.027.7745654
AACGCAG251300.027.5582526
CATGGGG362450.026.9941394
ATGGGGG265950.026.3595545
CTGGGGG119900.025.511215
ACGCAGA277700.024.0078457
Produced by FastQC (version 0.11.5)