FastQCFastQC Report
Mon 6 Jun 2016
ERR1211408_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211408_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670330
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268700.3099074660364715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193830.22355550480777547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141660.1633847846621755No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG125450.064.614452
ACCTGGG143400.055.2406233
GGTATCA133650.048.1974141
CCTGGGG114150.045.591044
GTACCTG202100.040.780041
TAACGCA12300.040.514324
GTATCAA245250.040.291511
CTGGGGG86600.039.466115
GTACATG327850.035.3145831
TGGGGGG240350.034.7774666
TACATGG335850.033.806412
TATAACG14700.033.6232072
ATAACGC15400.033.579933
ACATGGG333250.032.6861423
CATGGGG245950.032.647424
ATCAACG296900.032.63443
CAACGCA299850.032.3603675
ATGGGGG199500.032.18965
TCAACGC304400.031.8612174
GTACCCG13400.031.6491371
Produced by FastQC (version 0.11.5)