FastQCFastQC Report
Mon 6 Jun 2016
ERR1211408_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211408_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670330
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251150.289666021939188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189100.21810011844993213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137410.15848301045058263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG123650.062.102112
ACCTGGG135300.055.4358943
GGTATCA136700.046.882561
CCTGGGG108050.045.582964
CTGGGGG77150.042.936485
GTATCAA245000.040.3413661
GTACCTG207650.037.385151
TGGGGGG213100.036.8859336
TAACGCA9500.034.629044
GTACATG320950.033.0950361
ATGGGGG180800.032.5891045
ATCAACG303850.031.8922653
TACATGG327600.031.7457772
CAACGCA307950.031.5226975
CATGGGG229150.031.3173624
GTACCCG14300.031.2371621
TATCACG6800.031.0994032
TCAACGC312650.031.0855164
TATCAAC316350.030.8989662
ACATGGG323250.030.8069273
Produced by FastQC (version 0.11.5)