##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211408_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8670330 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07869677394056 33.0 31.0 34.0 30.0 34.0 2 32.20573426847652 33.0 31.0 34.0 30.0 34.0 3 32.28510160512921 34.0 31.0 34.0 30.0 34.0 4 35.933778299095884 37.0 35.0 37.0 35.0 37.0 5 35.801934182435964 37.0 35.0 37.0 35.0 37.0 6 35.83396998730152 37.0 35.0 37.0 35.0 37.0 7 35.76564040815055 37.0 35.0 37.0 33.0 37.0 8 35.737783452302274 37.0 35.0 37.0 33.0 37.0 9 37.3253113779983 39.0 37.0 39.0 34.0 39.0 10-11 37.292365169491816 39.0 37.0 39.0 34.0 39.0 12-13 37.203998290722495 39.0 37.0 39.0 33.0 39.0 14-15 38.411032452052 40.0 38.0 41.0 33.5 41.0 16-17 38.391676729720785 40.0 38.0 41.0 33.5 41.0 18-19 38.40212961905718 40.0 38.0 41.0 33.5 41.0 20-21 38.362696806234595 40.0 38.0 41.0 34.0 41.0 22-23 38.26355600075199 40.0 38.0 41.0 34.0 41.0 24-25 38.15622081281796 40.0 37.5 41.0 33.0 41.0 26-27 38.03932087936676 40.0 37.0 41.0 33.0 41.0 28-29 37.95233837697066 40.0 37.0 41.0 33.0 41.0 30-31 37.991500957864346 40.0 37.0 41.0 33.0 41.0 32-33 37.82576124553506 40.0 37.0 41.0 33.0 41.0 34-35 37.72686010797744 40.0 37.0 41.0 33.0 41.0 36-37 37.60019532128535 40.0 37.0 41.0 32.5 41.0 38-39 37.488754868615146 40.0 36.0 41.0 32.0 41.0 40-41 37.43380373065385 40.0 36.0 41.0 32.0 41.0 42-43 37.3129343404461 40.0 36.0 41.0 31.5 41.0 44-45 37.18728254864578 40.0 36.0 41.0 31.0 41.0 46-47 36.99301964285097 39.5 36.0 41.0 31.0 41.0 48-49 36.809887858939625 39.0 36.0 41.0 30.0 41.0 50-51 36.65324630089051 39.0 35.0 41.0 30.0 41.0 52-53 36.47676328351977 39.0 35.0 41.0 30.0 41.0 54-55 36.29434398690707 39.0 35.0 40.0 29.0 41.0 56-57 36.09257790649261 38.5 35.0 40.0 29.0 41.0 58-59 35.86463018131951 38.0 35.0 40.0 28.0 41.0 60-61 35.607776405281 38.0 34.0 40.0 28.0 41.0 62-63 35.307364713915156 38.0 34.0 40.0 27.5 41.0 64-65 34.97401419553812 37.0 34.0 40.0 27.0 41.0 66-67 34.62830180627496 36.5 34.0 39.0 26.0 41.0 68-69 34.26026391152355 36.0 33.0 39.0 26.0 40.0 70-71 33.87190770132163 35.5 33.0 39.0 26.0 40.0 72-73 33.49159524493301 35.0 32.5 38.0 25.5 40.0 74-75 33.09372342229189 35.0 32.0 37.0 25.0 39.0 76-77 32.05357789149894 34.0 30.5 36.0 24.0 39.0 78-79 32.53140578270954 35.0 32.0 36.0 25.0 39.0 80-81 32.42301705932761 35.0 32.0 36.0 25.0 37.5 82-83 32.12677470177029 35.0 32.0 35.5 24.5 37.0 84-85 31.82401379186259 34.0 31.0 35.0 24.0 37.0 86-87 31.565667454410615 34.0 31.0 35.0 23.5 36.0 88-89 31.348671215513136 34.0 31.0 35.0 23.0 36.0 90-91 31.125150196128637 34.0 31.0 35.0 22.5 35.5 92-93 30.92857503693631 34.0 31.0 35.0 20.5 35.0 94-95 30.754394065739135 34.0 30.0 35.0 20.0 35.0 96-97 30.586294004957136 34.0 30.0 35.0 20.0 35.0 98-99 30.403156165912947 34.0 30.0 35.0 19.5 35.0 100 28.58991756945814 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24685682061343428 1101 2 -0.24295285150065737 1101 3 -0.24488398159280322 1101 4 -0.13388422151412982 1101 5 -0.18689246796681402 1101 6 -0.19596840731662724 1101 7 -0.2772171202076663 1101 8 -0.32392873671664546 1101 9 -0.48175999004604364 1101 10-11 -0.4630046477325891 1101 12-13 -0.48191014014451383 1101 14-15 -0.5854543075207985 1101 16-17 -0.6528256125393099 1101 18-19 -0.6458233307651184 1101 20-21 -0.610554445038602 1101 22-23 -0.6603808595777139 1101 24-25 -0.7612054827184949 1101 26-27 -0.6956140609019315 1101 28-29 -0.6884294271417915 1101 30-31 -0.6287171601163521 1101 32-33 -0.5041721910441552 1101 34-35 -0.6237057305475915 1101 36-37 -0.5920360288904831 1101 38-39 -0.7093688645902247 1101 40-41 -0.723626967975818 1101 42-43 -0.6726909290368965 1101 44-45 -0.7282364375030355 1101 46-47 -0.7170220509889731 1101 48-49 -0.7041217606229324 1101 50-51 -0.7632116237907667 1101 52-53 -0.8015871609156946 1101 54-55 -0.7294298180467678 1101 56-57 -0.8231780962784114 1101 58-59 -0.8123063507780159 1101 60-61 -0.8292936249217249 1101 62-63 -0.8595193132184917 1101 64-65 -0.8122201570385528 1101 66-67 -0.7825662724909321 1101 68-69 -0.8725537349372345 1101 70-71 -0.8991085353129407 1101 72-73 -0.9359897516068472 1101 74-75 -0.9444376159618812 1101 76-77 -0.7330203221089953 1101 78-79 -0.5882324538321271 1101 80-81 -0.6280562859463501 1101 82-83 -0.7813794480894494 1101 84-85 -0.7528035526477588 1101 86-87 -0.8972266303207768 1101 88-89 -0.9434217819023374 1101 90-91 -1.02703848360035 1101 92-93 -1.1267339655869577 1101 94-95 -1.0788861138683465 1101 96-97 -1.0418998833018058 1101 98-99 -1.1952295005637232 1101 100 -1.2924543925059133 1102 1 -0.2927841623059777 1102 2 -0.2445447700573169 1102 3 -0.3511015536222075 1102 4 -0.2026634558209608 1102 5 -0.18366318256136793 1102 6 -0.22437236156370943 1102 7 -0.2568194353682358 1102 8 -0.29131037462396137 1102 9 -0.3948346038583992 1102 10-11 -0.4273836275289824 1102 12-13 -0.41804914804514937 1102 14-15 -0.5452614347956697 1102 16-17 -0.5936795736650495 1102 18-19 -0.5957343995124162 1102 20-21 -0.6685139149886865 1102 22-23 -0.6427456939002596 1102 24-25 -0.687162230684379 1102 26-27 -0.7517205916986711 1102 28-29 -0.7601965795729058 1102 30-31 -0.6854347861923742 1102 32-33 -0.6536669780022137 1102 34-35 -0.6437128057411741 1102 36-37 -0.566948571636452 1102 38-39 -0.6294455862206618 1102 40-41 -0.6048232898578192 1102 42-43 -0.6430291625968962 1102 44-45 -0.6759432640821785 1102 46-47 -0.6955133396568698 1102 48-49 -0.7281031047131776 1102 50-51 -0.7613777237868291 1102 52-53 -0.7368245729671159 1102 54-55 -0.8217518818414291 1102 56-57 -0.8250205946078779 1102 58-59 -0.8695583013695796 1102 60-61 -0.9724250368159133 1102 62-63 -0.929058885169276 1102 64-65 -0.8041739668337584 1102 66-67 -0.845346332237007 1102 68-69 -0.8814732693501881 1102 70-71 -0.9263572431074465 1102 72-73 -0.9886029055709216 1102 74-75 -0.9531317514125277 1102 76-77 -0.7636893209691031 1102 78-79 -0.6293674573697245 1102 80-81 -0.5826995045698311 1102 82-83 -0.6003994355113278 1102 84-85 -0.6236610022370037 1102 86-87 -0.7992622224751713 1102 88-89 -0.7252196180723018 1102 90-91 -0.7248073409565876 1102 92-93 -0.7358553149836027 1102 94-95 -0.8277775440401136 1102 96-97 -0.9591397716709729 1102 98-99 -0.9886983353186416 1102 100 -1.0310247612021115 1103 1 -0.35398662464088204 1103 2 -0.34073180131768765 1103 3 -0.3664965393526103 1103 4 -0.19109595731428897 1103 5 -0.18786234297817117 1103 6 -0.30619190095443116 1103 7 -0.35430815243201863 1103 8 -0.3459749561597647 1103 9 -0.5052717346740891 1103 10-11 -0.47409519087935337 1103 12-13 -0.5911069896858194 1103 14-15 -0.7531406161190972 1103 16-17 -0.8140222513712914 1103 18-19 -0.7812051302484662 1103 20-21 -0.7150848564501615 1103 22-23 -0.7200483254792118 1103 24-25 -0.677143854431641 1103 26-27 -0.8490346721393678 1103 28-29 -0.8769892805087238 1103 30-31 -0.7292102972430143 1103 32-33 -0.7165683103842397 1103 34-35 -0.7392195423177981 1103 36-37 -0.7320594098922371 1103 38-39 -0.7544727565266314 1103 40-41 -0.8239480741546217 1103 42-43 -0.7692570115382864 1103 44-45 -0.7494182010304726 1103 46-47 -0.8570109420329288 1103 48-49 -0.8268501595349775 1103 50-51 -0.8191841052828224 1103 52-53 -0.8945716360851819 1103 54-55 -0.9152760157101554 1103 56-57 -0.9600458152888436 1103 58-59 -1.0412669610231262 1103 60-61 -0.9508401990910897 1103 62-63 -0.9909926642892017 1103 64-65 -0.9266890245758503 1103 66-67 -1.075308335290039 1103 68-69 -1.0835479607287013 1103 70-71 -0.9888827541580909 1103 72-73 -0.9621481534636658 1103 74-75 -0.9469369216521244 1103 76-77 -0.8933418729037967 1103 78-79 -0.838682724177982 1103 80-81 -0.7447185279321431 1103 82-83 -0.7384055083858243 1103 84-85 -0.759002933278353 1103 86-87 -0.7905386875976248 1103 88-89 -0.852140620926221 1103 90-91 -0.9466539262091267 1103 92-93 -0.9301329581773388 1103 94-95 -0.9378216387504104 1103 96-97 -1.1472097258755234 1103 98-99 -1.2004626629883823 1103 100 -1.164584444616196 1104 1 -0.37894839508585676 1104 2 -0.3083279734065769 1104 3 -0.35270413381509513 1104 4 -0.17610743780803517 1104 5 -0.20297821681959505 1104 6 -0.3217417995248226 1104 7 -0.2966187555584767 1104 8 -0.30154283926000147 1104 9 -0.4398681869393073 1104 10-11 -0.4603235379560786 1104 12-13 -0.47264371435555574 1104 14-15 -0.6325311291593252 1104 16-17 -0.7264948291330526 1104 18-19 -0.7199720721358531 1104 20-21 -0.6867096017134173 1104 22-23 -0.672048442874221 1104 24-25 -0.7123619228700449 1104 26-27 -0.7773213754390582 1104 28-29 -0.852183828248485 1104 30-31 -0.744140937162868 1104 32-33 -0.7605423622391569 1104 34-35 -0.7527306729519836 1104 36-37 -0.6786915605029762 1104 38-39 -0.7908999935149126 1104 40-41 -0.86398479056502 1104 42-43 -0.7326090815634458 1104 44-45 -0.8380564414268932 1104 46-47 -0.7881418360199461 1104 48-49 -0.8546053697802876 1104 50-51 -0.8114633790182992 1104 52-53 -0.7835732076065725 1104 54-55 -0.7394408552967704 1104 56-57 -0.828746663146994 1104 58-59 -0.9498704913090421 1104 60-61 -0.9218956748062794 1104 62-63 -0.9968127782553822 1104 64-65 -0.9394994898594007 1104 66-67 -1.0099461324080536 1104 68-69 -0.9948570609745744 1104 70-71 -0.9862682214583671 1104 72-73 -0.8844942445227346 1104 74-75 -0.9477419755391594 1104 76-77 -0.8404635302133414 1104 78-79 -0.7947737287215837 1104 80-81 -0.8407496465894653 1104 82-83 -0.8460489014927788 1104 84-85 -0.8136197083509664 1104 86-87 -0.7114692627256964 1104 88-89 -0.7610504046291311 1104 90-91 -0.7956822259429508 1104 92-93 -0.9298848489666227 1104 94-95 -1.1079694040996024 1104 96-97 -1.0802611668591133 1104 98-99 -1.12334338244656 1104 100 -1.1218872238146353 1105 1 -0.3766094847949084 1105 2 -0.3393726764224816 1105 3 -0.374494124520087 1105 4 -0.17682202732911634 1105 5 -0.2659633073010852 1105 6 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-0.7970421562794741 1105 68-69 -0.885388676512072 1105 70-71 -0.9502620896758316 1105 72-73 -0.9421212851691152 1105 74-75 -0.9098672884055929 1105 76-77 -0.8995662178484274 1105 78-79 -0.8507433416641632 1105 80-81 -0.7325153230892241 1105 82-83 -0.7583757575490822 1105 84-85 -0.6847509443789548 1105 86-87 -0.6777917780845932 1105 88-89 -0.7709034538274082 1105 90-91 -0.718862788275576 1105 92-93 -0.753247343360318 1105 94-95 -0.7685972004826915 1105 96-97 -0.9013704269773157 1105 98-99 -0.8786009409132873 1105 100 -0.9149674678808424 1106 1 -0.3605901524489994 1106 2 -0.37410069016177516 1106 3 -0.3631616823616852 1106 4 -0.22273260183594346 1106 5 -0.24599399644488074 1106 6 -0.33256310615708884 1106 7 -0.3263188669686272 1106 8 -0.36902129057666855 1106 9 -0.47144146424954414 1106 10-11 -0.477731035874541 1106 12-13 -0.5287717108801004 1106 14-15 -0.7174263236543297 1106 16-17 -0.6902724092761972 1106 18-19 -0.6796323186843765 1106 20-21 -0.5707140519557967 1106 22-23 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0.9597429755139828 2311 34-35 0.9336621775420042 2311 36-37 0.9880478098998253 2311 38-39 1.0117880842569633 2311 40-41 1.0200906097316178 2311 42-43 1.1147468697596494 2311 44-45 1.0659716243599107 2311 46-47 1.0997064441128117 2311 48-49 1.073404136280523 2311 50-51 1.1045588604275878 2311 52-53 1.1164589218182428 2311 54-55 1.1044261808608056 2311 56-57 1.0974716413577212 2311 58-59 1.099161095051798 2311 60-61 1.1658862585940923 2311 62-63 1.1255083183566583 2311 64-65 1.1417327427156465 2311 66-67 1.1523174544511434 2311 68-69 1.146884865034572 2311 70-71 1.1147977177521042 2311 72-73 1.118954811636307 2311 74-75 0.9649553011539922 2311 76-77 0.9129392487497761 2311 78-79 0.8344291240756263 2311 80-81 0.8577912437995536 2311 82-83 0.9123930235058566 2311 84-85 0.9462503536848885 2311 86-87 0.9346515526668249 2311 88-89 0.973918539471029 2311 90-91 1.0323732362466131 2311 92-93 1.0763009736332592 2311 94-95 1.0647551187696678 2311 96-97 1.1060768120575055 2311 98-99 1.1663104595863523 2311 100 1.3493164511920703 2312 1 0.1549913800748257 2312 2 0.12276525988428943 2312 3 0.28701691219362857 2312 4 0.2424676257574916 2312 5 0.31861311333334896 2312 6 0.37727368087180935 2312 7 0.4376646687211334 2312 8 0.2583434193061507 2312 9 0.35714951266519535 2312 10-11 0.24204957706869834 2312 12-13 0.39410219634304866 2312 14-15 0.7620097319820687 2312 16-17 0.7858161999425661 2312 18-19 0.8198809701818988 2312 20-21 0.8061563480697345 2312 22-23 0.37885547342458636 2312 24-25 0.41832156627967976 2312 26-27 0.6942983307018267 2312 28-29 0.830184237156594 2312 30-31 0.8227111530390943 2312 32-33 0.7022979363508526 2312 34-35 0.8197754414312826 2312 36-37 0.905135951237547 2312 38-39 0.8967061398981713 2312 40-41 0.9268217143200772 2312 42-43 0.7318069046075948 2312 44-45 0.7258962983823878 2312 46-47 0.9178918158206528 2312 48-49 1.0051716157654838 2312 50-51 1.008915761177704 2312 52-53 1.0594442151437775 2312 54-55 1.090280125091894 2312 56-57 1.061198456611173 2312 58-59 1.0604137154116415 2312 60-61 1.0332562715627631 2312 62-63 1.0431078652211596 2312 64-65 1.1077770808738663 2312 66-67 1.170262770608936 2312 68-69 1.1418430063016132 2312 70-71 0.9389468420321734 2312 72-73 1.1247988118439523 2312 74-75 1.1490866383058673 2312 76-77 1.0313770280446128 2312 78-79 0.9297067463426316 2312 80-81 0.8321182905440168 2312 82-83 0.9040023540220403 2312 84-85 0.7163185994202337 2312 86-87 0.924271708032439 2312 88-89 0.4669930146008596 2312 90-91 0.7924547674897298 2312 92-93 1.0377738492376665 2312 94-95 1.1283067388533787 2312 96-97 1.204154700570868 2312 98-99 1.1750339272623016 2312 100 1.2843547685853132 2313 1 -0.3082586009572452 2313 2 0.015987521500413493 2313 3 0.29306805735527774 2313 4 0.13427370150608198 2313 5 0.2802408428655241 2313 6 0.3744425688932864 2313 7 0.13628620874488462 2313 8 0.11169030138482583 2313 9 0.303527755524307 2313 10-11 0.41190578826497415 2313 12-13 0.5985532168651488 2313 14-15 0.6340538635445583 2313 16-17 0.7588013505622158 2313 18-19 0.6288751213803607 2313 20-21 0.8104128650432898 2313 22-23 0.8448261086902349 2313 24-25 0.7177934085734279 2313 26-27 0.9093921897226664 2313 28-29 0.8348715908709465 2313 30-31 0.8211882791526506 2313 32-33 0.9434903770480574 2313 34-35 1.0184658099721062 2313 36-37 0.901527383366016 2313 38-39 0.6407177296584479 2313 40-41 0.7765467390499623 2313 42-43 0.853140743434146 2313 44-45 1.0330589973726276 2313 46-47 1.0680728962814001 2313 48-49 1.1161998872792935 2313 50-51 1.0210272801721132 2313 52-53 0.9818866779105733 2313 54-55 0.9953282177298135 2313 56-57 1.131055641255692 2313 58-59 1.0383800865922268 2313 60-61 1.0363504722448411 2313 62-63 1.1347926655425837 2313 64-65 1.1700730396798278 2313 66-67 1.210302075967725 2313 68-69 1.2459158708114515 2313 70-71 1.1313927214114372 2313 72-73 1.151040060949839 2313 74-75 0.8224006521908578 2313 76-77 0.9483253346025542 2313 78-79 0.7990990951348635 2313 80-81 0.9620097462929209 2313 82-83 0.9901437244749332 2313 84-85 1.0043957432849027 2313 86-87 1.0015252717358045 2313 88-89 1.0054539070333988 2313 90-91 1.1159879217531241 2313 92-93 0.8600020770417345 2313 94-95 0.9081520894212005 2313 96-97 1.1612868981827695 2313 98-99 1.2841330677170468 2313 100 1.398703472062003 2314 1 -0.3283270656577457 2314 2 -0.15598957914223632 2314 3 0.12323926018775921 2314 4 -0.17004581671131547 2314 5 0.13184003536679256 2314 6 0.19402792960742943 2314 7 0.1098100328633933 2314 8 0.1817027467368959 2314 9 0.31666651356779596 2314 10-11 0.44357160736425527 2314 12-13 0.5261747563507839 2314 14-15 0.581488692974709 2314 16-17 0.7420528713121755 2314 18-19 0.8134204126287727 2314 20-21 0.4364855139238415 2314 22-23 0.653994427808648 2314 24-25 0.8445318843549359 2314 26-27 0.7836509608578694 2314 28-29 0.7460319935981801 2314 30-31 0.7461554527981633 2314 32-33 0.8173514988137001 2314 34-35 0.878667930766234 2314 36-37 0.788172673721192 2314 38-39 0.8346528620909623 2314 40-41 0.9313161740250919 2314 42-43 0.6890537363236646 2314 44-45 0.6839467458448283 2314 46-47 0.8618627952505875 2314 48-49 0.8047676813192481 2314 50-51 0.8453007538658213 2314 52-53 0.9356217477564002 2314 54-55 0.9613184410749014 2314 56-57 0.8379781813105538 2314 58-59 0.8856645342137881 2314 60-61 0.9249759062690046 2314 62-63 0.6339478997231325 2314 64-65 0.8162239053424258 2314 66-67 0.9178646446633607 2314 68-69 1.0174413624511018 2314 70-71 1.073739112558961 2314 72-73 1.0845001952131028 2314 74-75 0.9934686232758523 2314 76-77 0.7998074550137524 2314 78-79 0.7961057620158627 2314 80-81 0.8044025780776778 2314 82-83 0.7778665661338806 2314 84-85 0.6891061886254448 2314 86-87 0.8294666399225044 2314 88-89 0.9654777597768458 2314 90-91 0.9613586811947314 2314 92-93 1.05712805286705 2314 94-95 1.0837881239485831 2314 96-97 1.1475002815663622 2314 98-99 1.1735101375374661 2314 100 1.1873836666151831 2315 1 -0.08222353811996186 2315 2 0.11778841617596214 2315 3 0.09439014047836736 2315 4 -0.11347325314579138 2315 5 0.19381398938116945 2315 6 0.3440036000324156 2315 7 0.42447872131945985 2315 8 0.3554509031322084 2315 9 0.5789441229368251 2315 10-11 0.5857855832125978 2315 12-13 0.6220033582168654 2315 14-15 0.753708539687338 2315 16-17 0.7068323566233659 2315 18-19 0.7857679546009777 2315 20-21 0.7936698951769046 2315 22-23 0.8604754993476931 2315 24-25 0.7896935124066573 2315 26-27 0.8904967936055712 2315 28-29 0.8960906359076546 2315 30-31 0.8734757819085459 2315 32-33 0.9371760100534701 2315 34-35 0.9243938781879635 2315 36-37 0.956447256155009 2315 38-39 0.9097637455478704 2315 40-41 0.8835372022072363 2315 42-43 0.9821774565979737 2315 44-45 0.8745598728777111 2315 46-47 0.8731656554165568 2315 48-49 0.9799347711799911 2315 50-51 0.9084442742572989 2315 52-53 0.8730672393004184 2315 54-55 0.8948236433365153 2315 56-57 1.0434681137972746 2315 58-59 1.0412945736548238 2315 60-61 1.009618265570026 2315 62-63 1.0072617997377975 2315 64-65 1.080785775097901 2315 66-67 1.070403681986086 2315 68-69 0.984011219987103 2315 70-71 0.6572226477899292 2315 72-73 0.9812621653677027 2315 74-75 1.126725606383495 2315 76-77 1.0072966542965567 2315 78-79 0.9314489085234641 2315 80-81 0.928990408838466 2315 82-83 0.9174908076035422 2315 84-85 0.8514505384925428 2315 86-87 0.8425065108703613 2315 88-89 0.9521089033722703 2315 90-91 0.983398016611865 2315 92-93 1.070975678971969 2315 94-95 1.1003706069199062 2315 96-97 1.1210065646920064 2315 98-99 1.099698713928607 2315 100 0.961871815031337 2316 1 0.4755979475999439 2316 2 0.4730615696224092 2316 3 0.45999992402988 2316 4 0.3197350490397213 2316 5 0.4059905769249781 2316 6 0.4278960017618303 2316 7 0.48691537860218403 2316 8 0.4790411389634315 2316 9 0.5273304127948322 2316 10-11 0.4781212793697449 2316 12-13 0.6459230464836239 2316 14-15 0.7668874393146865 2316 16-17 0.7574732794637029 2316 18-19 0.8144647608697326 2316 20-21 0.881230144444082 2316 22-23 0.8758462868384171 2316 24-25 0.9281758441556036 2316 26-27 0.923752490835696 2316 28-29 0.923761258609062 2316 30-31 0.835830855754395 2316 32-33 0.7324713213277363 2316 34-35 0.6524054510083204 2316 36-37 0.621444574443835 2316 38-39 0.6196446812494685 2316 40-41 0.7074929140248898 2316 42-43 0.8058528650613752 2316 44-45 0.73620590308348 2316 46-47 0.7666500382887662 2316 48-49 0.5279808554382157 2316 50-51 0.5477162289738047 2316 52-53 0.7324763950185655 2316 54-55 0.8006357583543036 2316 56-57 0.8543600921062051 2316 58-59 0.8131189091705693 2316 60-61 0.834563385674123 2316 62-63 0.9016588495382294 2316 64-65 0.8719013323324631 2316 66-67 0.88930578517882 2316 68-69 0.84984891113114 2316 70-71 0.749225098610026 2316 72-73 0.7853038011869913 2316 74-75 0.7862350973263545 2316 76-77 0.6795105085399662 2316 78-79 0.663437989365697 2316 80-81 0.626397962452323 2316 82-83 0.5786915107901791 2316 84-85 0.5978751970106231 2316 86-87 0.6406253615115993 2316 88-89 0.6189124107389716 2316 90-91 0.6272168832827951 2316 92-93 0.6510234414088458 2316 94-95 0.588221072642785 2316 96-97 0.7839370130454135 2316 98-99 0.8379010956544377 2316 100 0.6014349614695682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 16.0 12 56.0 13 232.0 14 955.0 15 2929.0 16 6930.0 17 14181.0 18 22781.0 19 31720.0 20 41846.0 21 54272.0 22 65574.0 23 68924.0 24 71716.0 25 79065.0 26 93003.0 27 112678.0 28 138366.0 29 167873.0 30 206141.0 31 253369.0 32 319504.0 33 415342.0 34 584584.0 35 770307.0 36 1148942.0 37 1863424.0 38 1849101.0 39 286445.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.701069619671806 19.358244352220733 12.901453562037062 24.0392324660704 2 15.679553142729286 21.07626814665647 41.32949956922055 21.914679141393695 3 17.60354191865153 28.398449470602554 31.29723220422201 22.700776406523904 4 11.845785099408642 17.570364467593446 38.757050520114284 31.826799912883626 5 11.872827205631742 38.27855112786057 34.16911582112063 15.679505845387066 6 28.113258447286142 37.48766626964213 20.758727840662104 13.640347442409626 7 26.50890388436778 33.598033382170456 23.326494290808515 16.56656844265325 8 26.296255159710103 35.71185459147161 21.160206281141498 16.831683967676792 9 28.622806662424537 15.851406493777462 20.472465556354848 35.053321287443154 10-11 24.65847013443208 26.012104377127677 29.88490991957327 19.444515568866972 12-13 24.867579568460197 24.352839653433023 28.871858081974317 21.907722696132463 14-15 22.859574796915112 25.90277587553642 27.02320644750732 24.21444288004115 16-17 21.92066997269479 27.87598409678716 27.87451356342125 22.328832367096798 18-19 21.5217586873856 27.479997877820107 29.375825372275333 21.622418062518957 20-21 22.665631719217068 27.423433543888624 28.5368747604978 21.37405997639651 22-23 21.999031294024064 27.220332283796132 28.686435734651145 22.094200687528655 24-25 21.50932883246347 26.716129639446002 29.151332752962865 22.623208775127665 26-27 21.549281552596305 26.861911864123716 29.037325402376624 22.551481180903355 28-29 21.47548391643283 28.15305607787183 28.738529594370267 21.632930411325074 30-31 23.21807442392892 26.933723171587943 28.998954760596924 20.849247643886216 32-33 22.698373313852642 26.87050911992333 28.876536968037154 21.55458059818687 34-35 22.53409768994297 27.379100324544886 28.87778648447777 21.209015501034372 36-37 22.18441270159918 27.331708887265982 28.73883337544433 21.74504503569051 38-39 22.042827571930083 27.792244902220446 28.572535204890304 21.59239232095917 40-41 22.234647116440662 26.75584832966036 29.346220596881256 21.663283957017722 42-43 21.998614908367866 27.172610917333994 28.34621339372882 22.48256078056932 44-45 20.915440767242806 27.593328784812478 29.292420484031055 22.198809963913657 46-47 20.88487296399654 26.62908682247489 28.898123478251343 23.587916735277233 48-49 21.137330609337805 26.900749579361843 29.094976996463323 22.866942814837028 50-51 21.251393038117005 27.222994121715093 29.029296608821976 22.49631623134592 52-53 21.015430758840907 27.636831282306744 28.366821523917856 22.98091643493449 54-55 20.79488997243659 26.95279085304466 29.225578420995806 23.02674075352294 56-57 21.298105893735563 27.123727887815548 28.40284548750049 23.1753207309484 58-59 20.675721439769827 27.259133284918995 29.00659983703992 23.058545438271256 60-61 21.779195252883458 27.50603663276333 28.3980701921221 22.316697922231114 62-63 21.540087080599292 27.32764079809293 28.387316919069228 22.744955202238554 64-65 21.085525991344323 26.744380076449083 28.230471530990624 23.939622401215967 66-67 20.85121935951569 26.774474669648317 27.96750528554034 24.406800685295654 68-69 20.373734310364604 27.039995919419617 28.41115055518281 24.17511921503297 70-71 20.78461757086726 26.62571449127852 28.591207740879675 23.99846019697455 72-73 20.740737022814688 27.1888284834879 27.683029316917434 24.387405176779982 74-75 21.138936989912274 27.094691329488573 27.397760945512132 24.36861073508702 76-77 20.58897077787473 26.562748676432086 27.942925768230094 24.905354777463092 78-79 20.274343066388056 26.524164994569894 28.282084216002414 24.919407723039637 80-81 20.413583338975297 26.794844749532043 27.62355003967059 25.16802187182207 82-83 21.02122866640624 26.713846095629602 27.61494073190952 24.649984506054636 84-85 21.000614465025976 27.03524952184236 27.047319576624947 24.91681643650672 86-87 20.30433891322433 26.999013792879428 27.54946772336743 25.14717957052881 88-89 20.307695326001593 26.317304024839977 27.801505239718338 25.57349540944009 90-91 21.17443011883503 25.89622392524518 27.745491875034432 25.183854080885354 92-93 20.906625414556366 26.190479211989015 27.55308288496967 25.349812488484947 94-95 20.300781149523875 25.82449897971766 27.674894710391722 26.199825160366746 96-97 20.195014032020676 25.664408090826846 27.990027441203374 26.1505504359491 98-99 20.04091094247446 25.770040657145355 27.79729131560468 26.39175708477551 100 19.813986411207143 25.868956675196287 28.094721947476266 26.222334966120304 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1148.0 1 824.0 2 1523.0 3 2424.0 4 1757.5 5 1324.0 6 1794.0 7 3055.0 8 4678.0 9 5532.0 10 5337.5 11 5102.5 12 5547.5 13 6312.5 14 7080.5 15 7829.5 16 8575.0 17 9475.5 18 10507.5 19 11499.0 20 12882.5 21 14505.5 22 16785.5 23 20042.0 24 24140.5 25 30251.5 26 39616.5 27 50707.5 28 61450.0 29 75710.0 30 95713.5 31 115319.0 32 139234.0 33 164463.0 34 189061.5 35 211266.0 36 233490.0 37 259294.5 38 269494.5 39 270413.0 40 269271.5 41 267558.5 42 268291.0 43 272711.5 44 283705.0 45 299161.5 46 314150.0 47 325137.0 48 339376.0 49 357800.5 50 376676.5 51 387006.5 52 379938.0 53 358931.0 54 328490.0 55 287266.0 56 247221.0 57 212917.5 58 178667.5 59 142866.5 60 104532.0 61 74371.0 62 55408.0 63 41065.0 64 29016.5 65 20173.0 66 14257.5 67 10850.5 68 8149.0 69 5713.0 70 4257.5 71 3136.0 72 2355.5 73 1736.0 74 1269.5 75 875.0 76 553.5 77 329.0 78 194.0 79 118.0 80 75.5 81 41.0 82 30.0 83 25.5 84 16.5 85 11.0 86 12.5 87 8.5 88 3.5 89 2.5 90 2.0 91 1.0 92 1.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.051278325046451516 2 0.0 3 1.1533586380218515E-5 4 0.016908237633400342 5 0.003817617091852329 6 1.1533586380218515E-5 7 1.499366229428407E-4 8 3.1140683226589993E-4 9 0.001764638716173433 10-11 8.996197376570442E-4 12-13 3.3447400502633695E-4 14-15 2.8833965950546285E-5 16-17 7.496831147142036E-5 18-19 0.0 20-21 2.8833965950546285E-5 22-23 5.766793190109258E-6 24-25 6.920151828131109E-5 26-27 1.7300379570327772E-5 28-29 9.572876695581368E-4 30-31 7.785170806647498E-4 32-33 0.01422091200680943 34-35 0.023499682249695227 36-37 0.0318326984094031 38-39 0.0210545619370889 40-41 1.4416982975273143E-4 42-43 0.001678136818321794 44-45 0.009745880491284645 46-47 0.01421514521361932 48-49 0.005391951632752156 50-51 0.010143789221402185 52-53 0.015899048825131223 54-55 0.009988085805269234 56-57 0.0029006969746249566 58-59 0.008327249366517768 60-61 0.0016204688864207013 62-63 9.976552218889016E-4 64-65 0.01942832625747809 66-67 0.04610551155492351 68-69 0.05646843891754986 70-71 0.049438718018806664 72-73 0.04164201362577895 74-75 0.029058870884960546 76-77 0.029589415858450603 78-79 0.012398605358734905 80-81 0.002064511962059114 82-83 0.028003547731170555 84-85 0.04907541004782978 86-87 0.04960018822812972 88-89 0.06072433229185048 90-91 0.03532160828941921 92-93 0.026861722679528924 94-95 0.007739036461126624 96-97 0.0039041189897039675 98-99 0.022940303310254626 100 0.019791634228454973 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8670330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.18914281920159 #Duplication Level Percentage of deduplicated Percentage of total 1 78.61575862169055 46.5319936489912 2 13.368901497569402 15.825876401509467 3 3.8659555135252854 6.864677790681838 4 1.5172601782901494 3.5922131754681166 5 0.7392757690142508 2.1878549537479786 6 0.4382306635929245 1.5563098401093056 7 0.285153574722458 1.1814596961757406 8 0.19105880261839334 0.9046885404036583 9 0.14668903373109177 0.7814158350768242 >10 0.736300775804114 7.763736222531173 >50 0.04730232595238341 1.951032181051536 >100 0.04012358042565587 4.865082866970389 >500 0.005463954425751729 2.2127744063124504 >1k 0.0023195283410988397 2.3891513943446894 >5k 1.288626853410672E-4 0.5105390710093736 >10k+ 7.731761120464032E-5 0.8811939756163342 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25115 0.289666021939188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18910 0.21810011844993213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13741 0.15848301045058263 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1533586380218515E-5 0.0 3 0.0 0.0 0.0 1.1533586380218515E-5 0.0 4 0.0 0.0 0.0 2.306717276043703E-5 0.0 5 1.1533586380218515E-5 0.0 0.0 3.4600759140655544E-5 0.0 6 2.306717276043703E-5 0.0 0.0 3.4600759140655544E-5 0.0 7 2.306717276043703E-5 0.0 0.0 3.4600759140655544E-5 0.0 8 2.306717276043703E-5 0.0 0.0 6.920151828131109E-5 0.0 9 2.306717276043703E-5 0.0 0.0 9.226869104174812E-5 1.1533586380218515E-5 10-11 3.4600759140655544E-5 0.0 0.0 1.3840303656262218E-4 1.1533586380218515E-5 12-13 3.4600759140655544E-5 0.0 0.0 2.01837761653824E-4 2.306717276043703E-5 14-15 4.03675523307648E-5 0.0 0.0 2.479721071746981E-4 2.883396595054629E-5 16-17 4.613434552087406E-5 0.0 0.0 3.1717362545600916E-4 3.4600759140655544E-5 18-19 4.613434552087406E-5 0.0 0.0 4.382762824483036E-4 4.613434552087406E-5 20-21 4.613434552087406E-5 0.0 0.0 9.226869104174812E-4 5.766793190109258E-5 22-23 4.613434552087406E-5 0.0 0.0 0.002283650103283266 6.920151828131109E-5 24-25 8.07351046615296E-5 0.0 0.0 0.005807160742440022 8.073510466152961E-5 26-27 1.095690706120759E-4 0.0 0.0 0.010553231537899941 8.073510466152961E-5 28-29 1.1533586380218515E-4 0.0 0.0 0.023961025704903965 8.073510466152961E-5 30-31 1.4993662294284069E-4 0.0 0.0 0.07546425568576974 8.073510466152961E-5 32-33 1.8453738208349624E-4 0.0 0.0 0.19132489766825483 1.0380227742196663E-4 34-35 1.9607096846371475E-4 5.766793190109258E-6 0.0 0.3503269195059473 1.0380227742196663E-4 36-37 2.0760455484393326E-4 1.1533586380218515E-5 0.0 0.5817483302250318 1.0380227742196663E-4 38-39 2.0760455484393326E-4 1.1533586380218515E-5 0.0 0.9887512932033728 1.0380227742196663E-4 40-41 2.0760455484393326E-4 1.1533586380218515E-5 0.0 1.5271967733638743 1.0380227742196663E-4 42-43 2.0760455484393326E-4 1.1533586380218515E-5 0.0 2.0141217231639397 1.095690706120759E-4 44-45 2.191381412241518E-4 2.306717276043703E-5 0.0 2.5215072552025126 1.1533586380218515E-4 46-47 2.306717276043703E-4 3.4600759140655544E-5 0.0 3.0666076147044 1.1533586380218515E-4 48-49 2.306717276043703E-4 3.4600759140655544E-5 0.0 3.664387629997935 1.1533586380218515E-4 50-51 2.306717276043703E-4 3.4600759140655544E-5 0.0 4.323624360318465 1.1533586380218515E-4 52-53 2.306717276043703E-4 3.4600759140655544E-5 0.0 5.002058745168869 1.8453738208349624E-4 54-55 2.306717276043703E-4 3.4600759140655544E-5 0.0 5.7426303266427 1.9607096846371475E-4 56-57 2.306717276043703E-4 4.613434552087406E-5 0.0 6.474672820988359 1.9607096846371475E-4 58-59 2.306717276043703E-4 5.766793190109258E-5 0.0 7.301423359895183 1.9607096846371475E-4 60-61 2.6527248674502584E-4 7.496831147142034E-5 0.0 8.120221490992845 1.9607096846371475E-4 62-63 2.6527248674502584E-4 8.073510466152961E-5 0.0 9.008146172060348 2.0760455484393326E-4 64-65 2.6527248674502584E-4 8.073510466152961E-5 0.0 9.806910463615571 2.0760455484393326E-4 66-67 2.6527248674502584E-4 8.073510466152961E-5 0.0 10.641353904638002 2.0760455484393326E-4 68-69 2.6527248674502584E-4 8.073510466152961E-5 0.0 11.586571676049239 2.0760455484393326E-4 70-71 2.6527248674502584E-4 8.073510466152961E-5 0.0 12.581378102102226 2.0760455484393326E-4 72-73 2.6527248674502584E-4 8.073510466152961E-5 0.0 13.462019323370622 2.306717276043703E-4 74-75 2.6527248674502584E-4 8.073510466152961E-5 0.0 14.306912193653528 2.479721071746981E-4 76-77 2.7680607312524435E-4 8.073510466152961E-5 0.0 15.204334783105141 2.6527248674502584E-4 78-79 2.998732458856814E-4 8.073510466152961E-5 0.0 16.173092604318406 2.6527248674502584E-4 80-81 2.998732458856814E-4 8.073510466152961E-5 0.0 17.057309237364667 2.7680607312524435E-4 82-83 2.998732458856814E-4 8.073510466152961E-5 0.0 17.965515730081783 2.8833965950546286E-4 84-85 2.998732458856814E-4 8.073510466152961E-5 0.0 18.87315131027308 2.8833965950546286E-4 86-87 2.998732458856814E-4 8.073510466152961E-5 0.0 19.78102332898517 2.8833965950546286E-4 88 2.998732458856814E-4 8.073510466152961E-5 0.0 20.46226614211916 2.8833965950546286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 12365 0.0 62.10211 2 ACCTGGG 13530 0.0 55.435894 3 GGTATCA 13670 0.0 46.88256 1 CCTGGGG 10805 0.0 45.58296 4 CTGGGGG 7715 0.0 42.93648 5 GTATCAA 24500 0.0 40.341366 1 GTACCTG 20765 0.0 37.38515 1 TGGGGGG 21310 0.0 36.885933 6 TAACGCA 950 0.0 34.62904 4 GTACATG 32095 0.0 33.095036 1 ATGGGGG 18080 0.0 32.589104 5 ATCAACG 30385 0.0 31.892265 3 TACATGG 32760 0.0 31.745777 2 CAACGCA 30795 0.0 31.522697 5 CATGGGG 22915 0.0 31.317362 4 GTACCCG 1430 0.0 31.237162 1 TATCACG 680 0.0 31.099403 2 TCAACGC 31265 0.0 31.085516 4 TATCAAC 31635 0.0 30.898966 2 ACATGGG 32325 0.0 30.806927 3 >>END_MODULE