FastQCFastQC Report
Mon 6 Jun 2016
ERR1211407_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211407_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9028876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253850.2811534902018812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188900.20921762575983988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123170.13641786640994957No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG102450.053.2941972
ACCTGGG117400.046.287883
GGTATCA151550.043.0587771
GTATCAA259050.038.2129251
CCTGGGG114300.034.1355634
GTACCTG165300.033.6338841
GTACATG302000.032.4351121
ATCAACG312200.030.9726943
CAACGCA312200.030.942415
TACATGG307450.030.8791452
TATCAAC322700.030.265862
TCAACGC322050.030.1127974
GTACACG14250.029.7574371
AACGCAG334050.029.2152966
ACATGGG313600.028.9906523
TGGGGGG212300.028.6757136
CATGGGG241600.028.0960624
CTGGGGG89800.027.953755
TAACGCA11100.027.5274244
ATGGGGG188300.027.3612175
Produced by FastQC (version 0.11.5)