FastQCFastQC Report
Mon 6 Jun 2016
ERR1211407_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211407_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9028876
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234420.25963364653584786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187150.20727940000505046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125730.13925321379981295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG103500.054.4841842
ACCTGGG121050.045.2660873
GGTATCA151550.043.0944441
GTATCAA256350.038.6278081
CCTGGGG108450.036.0099684
GTACCTG170850.033.6303371
TAACGCA8950.033.605334
ATCAACG312000.031.027863
CTGGGGG76550.030.936025
CAACGCA314050.030.8958645
TGGGGGG185050.030.6725376
GTACATG298750.030.4542621
TATCAAC323600.030.321562
TCAACGC324350.029.93424
ATAACGC10150.029.6314663
TACATGG303550.029.3210372
AACGCAG336950.029.2000146
ACATGGG301850.028.2102033
ATGGGGG177700.027.5259515
CATGGGG229450.027.44534
Produced by FastQC (version 0.11.5)