FastQCFastQC Report
Mon 6 Jun 2016
ERR1211406_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211406_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9216684
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256120.27788736165848804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196620.2133305210420581No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123460.13395273180679734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100550.050.4692462
ACCTGGG115700.043.0674553
GGTATCA154800.042.0685121
GTATCAA265550.038.364491
GTACCTG157800.032.7283131
CCTGGGG111850.032.403214
TAACGCA9900.032.2881664
ATCAACG316300.031.135913
GTACATG321450.031.0919041
CAACGCA317800.031.0775285
TATCAAC328850.030.5482752
TCAACGC331000.029.8520664
ATAACGC10400.029.832343
TACATGG326850.029.7990282
AACGCAG338600.029.7107076
ACATGGG327450.028.7549533
CATGGGG252650.028.1879184
TGGGGGG228900.027.601946
ATGGGGG202050.027.1277685
ACGCAGA374150.026.410437
Produced by FastQC (version 0.11.5)