FastQCFastQC Report
Mon 6 Jun 2016
ERR1211406_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211406_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9216684
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237210.2573702212205604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192520.208882066478573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125060.13568871407547445No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG100750.052.0052452
ACCTGGG114750.044.9649433
GGTATCA166700.041.2055661
GTATCAA277550.036.318241
CCTGGGG109700.034.2270364
GTACCTG172200.030.8522261
ATCAACG329300.029.953333
CAACGCA332200.029.674645
TGGGGGG189100.029.0947346
TATCAAC342800.029.088542
CTGGGGG79200.029.011045
GTACATG318700.028.9144731
TCAACGC342100.028.8878564
AACGCAG353450.028.5399426
TACATGG323450.027.9957332
ACATGGG318750.027.111653
CATGGGG236400.026.4184064
ATGGGGG180500.025.849475
TAACGCA10700.025.4726034
ACGCAGA393700.025.2163987
Produced by FastQC (version 0.11.5)