##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211406_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9216684 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.098702743850176 33.0 31.0 34.0 30.0 34.0 2 32.237442555261744 33.0 31.0 34.0 30.0 34.0 3 32.317348517102246 34.0 31.0 34.0 30.0 34.0 4 35.95390478831649 37.0 35.0 37.0 35.0 37.0 5 35.81682370796265 37.0 35.0 37.0 35.0 37.0 6 35.84963757030186 37.0 35.0 37.0 35.0 37.0 7 35.77936045111235 37.0 35.0 37.0 33.0 37.0 8 35.757944505854816 37.0 35.0 37.0 33.0 37.0 9 37.35162483600393 39.0 37.0 39.0 34.0 39.0 10-11 37.32555342029737 39.0 37.0 39.0 34.0 39.0 12-13 37.24182558499348 39.0 37.0 39.0 33.0 39.0 14-15 38.46809405638731 40.0 38.0 41.0 34.0 41.0 16-17 38.45720668084096 40.0 38.0 41.0 34.0 41.0 18-19 38.45546619586827 40.0 38.0 41.0 34.0 41.0 20-21 38.41438390423281 40.0 38.0 41.0 34.0 41.0 22-23 38.320044768812735 40.0 38.0 41.0 34.0 41.0 24-25 38.21073121309139 40.0 38.0 41.0 33.5 41.0 26-27 38.09383450707435 40.0 37.0 41.0 33.0 41.0 28-29 38.014804673785065 40.0 37.0 41.0 33.0 41.0 30-31 38.0572185723195 40.0 37.0 41.0 33.0 41.0 32-33 37.90501258370147 40.0 37.0 41.0 33.0 41.0 34-35 37.80216480243871 40.0 37.0 41.0 33.0 41.0 36-37 37.66841002685999 40.0 37.0 41.0 33.0 41.0 38-39 37.55860665289165 40.0 37.0 41.0 32.0 41.0 40-41 37.50071690642751 40.0 37.0 41.0 32.0 41.0 42-43 37.37707455306052 40.0 36.0 41.0 32.0 41.0 44-45 37.238677869394245 40.0 36.0 41.0 31.0 41.0 46-47 37.034412864757 39.5 36.0 41.0 31.0 41.0 48-49 36.83431649604131 39.0 36.0 41.0 30.5 41.0 50-51 36.66372130150063 39.0 35.0 41.0 30.0 41.0 52-53 36.46692335334487 39.0 35.0 40.5 30.0 41.0 54-55 36.27786202716726 39.0 35.0 40.0 29.0 41.0 56-57 36.05660810330484 38.0 35.0 40.0 29.0 41.0 58-59 35.802159268995226 38.0 35.0 40.0 28.0 41.0 60-61 35.546037707270855 38.0 34.0 40.0 28.0 41.0 62-63 35.2517120040136 37.0 34.0 40.0 28.0 41.0 64-65 34.90964423864375 37.0 34.0 40.0 27.0 41.0 66-67 34.5577996381345 36.0 33.5 39.0 26.0 41.0 68-69 34.20006989498609 36.0 33.0 39.0 26.0 40.0 70-71 33.8240691012082 35.0 33.0 38.5 26.0 40.0 72-73 33.460655969109936 35.0 32.5 37.5 26.0 40.0 74-75 33.08727591181383 35.0 32.0 37.0 25.0 39.0 76-77 32.06720226059611 34.0 30.5 36.0 24.5 39.0 78-79 32.533894782548685 35.0 32.0 36.0 25.0 38.5 80-81 32.42458659752249 35.0 32.0 36.0 25.0 37.5 82-83 32.148194676089574 35.0 32.0 35.0 25.0 37.0 84-85 31.8690262680157 34.0 31.0 35.0 24.0 36.5 86-87 31.616928170695665 34.0 31.0 35.0 24.0 36.0 88-89 31.39653231031898 34.0 31.0 35.0 23.0 36.0 90-91 31.180055755410514 34.0 31.0 35.0 23.0 35.5 92-93 31.002740627757227 34.0 31.0 35.0 22.5 35.0 94-95 30.831348183359655 34.0 30.0 35.0 20.0 35.0 96-97 30.66353772137571 34.0 30.0 35.0 20.0 35.0 98-99 30.472995927819596 34.0 30.0 35.0 20.0 35.0 100 28.704260990178245 32.0 26.0 34.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.17892610909855833 1101 2 -0.215786453986631 1101 3 -0.24037396227028296 1101 4 -0.11292625621139507 1101 5 -0.09897350523225157 1101 6 -0.20194833499176212 1101 7 -0.25926538410066513 1101 8 -0.28548876779380095 1101 9 -0.42579504252324796 1101 10-11 -0.38556260588905644 1101 12-13 -0.3393101454057117 1101 14-15 -0.48826655048638656 1101 16-17 -0.4886504241863179 1101 18-19 -0.5409989704893619 1101 20-21 -0.5838525934427707 1101 22-23 -0.63881548123328 1101 24-25 -0.6619196418357802 1101 26-27 -0.6685123576154268 1101 28-29 -0.6751501792185266 1101 30-31 -0.5762230682954552 1101 32-33 -0.4474200071155039 1101 34-35 -0.5465202516788352 1101 36-37 -0.6412470605234049 1101 38-39 -0.6663275818642873 1101 40-41 -0.6596383305980851 1101 42-43 -0.6106590822504145 1101 44-45 -0.7483643629728363 1101 46-47 -0.663154962579938 1101 48-49 -0.6333597206487696 1101 50-51 -0.6938471695476522 1101 52-53 -0.6997549744172673 1101 54-55 -0.6952119150694998 1101 56-57 -0.7341387090636502 1101 58-59 -0.7426454711870889 1101 60-61 -0.732067718406789 1101 62-63 -0.7207041726706436 1101 64-65 -0.7651719466629388 1101 66-67 -0.7696339140999484 1101 68-69 -0.8266418092274819 1101 70-71 -0.8211487213841266 1101 72-73 -0.9236999033842679 1101 74-75 -0.91345898002551 1101 76-77 -0.7450652916018896 1101 78-79 -0.6593712621316641 1101 80-81 -0.6426383435224281 1101 82-83 -0.7700609630564799 1101 84-85 -0.8415262315854939 1101 86-87 -0.9331147899051686 1101 88-89 -0.8412785308361848 1101 90-91 -0.8784510487325825 1101 92-93 -1.0432590352678766 1101 94-95 -1.0750023226287198 1101 96-97 -1.050246825579574 1101 98-99 -1.183660394331433 1101 100 -1.11635471000789 1102 1 -0.328028277116168 1102 2 -0.24008431771241945 1102 3 -0.25477088772796463 1102 4 -0.14248746882083196 1102 5 -0.11357520850066294 1102 6 -0.22274768286641233 1102 7 -0.24614325116221636 1102 8 -0.2874875353668713 1102 9 -0.45423599814373716 1102 10-11 -0.4439185121875866 1102 12-13 -0.46745644463794633 1102 14-15 -0.5492428429208402 1102 16-17 -0.6408749149285384 1102 18-19 -0.6438151054119956 1102 20-21 -0.664350961532989 1102 22-23 -0.6431281408244374 1102 24-25 -0.7133188237066861 1102 26-27 -0.8062019187006513 1102 28-29 -0.7648112157838725 1102 30-31 -0.6431747075560068 1102 32-33 -0.6513735040924828 1102 34-35 -0.6484855416859503 1102 36-37 -0.5696787148391493 1102 38-39 -0.6095832210213743 1102 40-41 -0.6749704836916237 1102 42-43 -0.7403097082911145 1102 44-45 -0.6047419746172125 1102 46-47 -0.6842939482703088 1102 48-49 -0.6614090457968871 1102 50-51 -0.7366043631441741 1102 52-53 -0.6426238695689293 1102 54-55 -0.675146388114932 1102 56-57 -0.689188399408323 1102 58-59 -0.7216186068180193 1102 60-61 -0.8532721552020917 1102 62-63 -0.920994634638852 1102 64-65 -0.8381804873157748 1102 66-67 -0.7991842111687788 1102 68-69 -0.8671188217553549 1102 70-71 -0.9077011708160327 1102 72-73 -0.9475207004501627 1102 74-75 -0.9365244816482488 1102 76-77 -0.8114815831572599 1102 78-79 -0.7005554833781815 1102 80-81 -0.6483976404390894 1102 82-83 -0.7028775205004827 1102 84-85 -0.7128776878280085 1102 86-87 -0.7873631418084521 1102 88-89 -0.797788464440039 1102 90-91 -0.7683940449757038 1102 92-93 -0.8808421524094854 1102 94-95 -0.9204361769566738 1102 96-97 -0.9847633954107273 1102 98-99 -1.0474310602982086 1102 100 -1.0641470163945215 1103 1 -0.3530330767349241 1103 2 -0.2943271207755451 1103 3 -0.34101645754110166 1103 4 -0.17568876848947212 1103 5 -0.1657754931224673 1103 6 -0.2739900935086723 1103 7 -0.3079512404272151 1103 8 -0.3449921555517861 1103 9 -0.4612840427689662 1103 10-11 -0.4377496697358012 1103 12-13 -0.48613430923773393 1103 14-15 -0.7146159618770866 1103 16-17 -0.7754626579510173 1103 18-19 -0.7013209318085885 1103 20-21 -0.7078514010670602 1103 22-23 -0.7843252694919371 1103 24-25 -0.7805302234508531 1103 26-27 -0.8574552167829594 1103 28-29 -0.8730478929314955 1103 30-31 -0.7243600695891743 1103 32-33 -0.7189238177832067 1103 34-35 -0.7337861431961201 1103 36-37 -0.686451602240588 1103 38-39 -0.741895686167382 1103 40-41 -0.7831912041566582 1103 42-43 -0.7343467732681148 1103 44-45 -0.7731846657817414 1103 46-47 -0.8317158308485517 1103 48-49 -0.8774750868480652 1103 50-51 -0.8888445725841478 1103 52-53 -0.8859535347573697 1103 54-55 -0.9048281465867589 1103 56-57 -0.9238923739341374 1103 58-59 -1.0483451719068384 1103 60-61 -0.92111361528017 1103 62-63 -0.9295865119557973 1103 64-65 -0.876546288713989 1103 66-67 -0.9657499265901137 1103 68-69 -0.8787697622598927 1103 70-71 -0.8720601542958875 1103 72-73 -0.9197213527694359 1103 74-75 -1.0148408100773878 1103 76-77 -0.937532579831835 1103 78-79 -0.6883445453056254 1103 80-81 -0.7213184815442091 1103 82-83 -0.7568545864588501 1103 84-85 -0.71052207467703 1103 86-87 -0.724434069451771 1103 88-89 -0.7611832038812878 1103 90-91 -0.8571826085879373 1103 92-93 -0.8521828073373356 1103 94-95 -0.8716345317028633 1103 96-97 -0.9563195128559485 1103 98-99 -1.1202157965771313 1103 100 -1.2089931245974803 1104 1 -0.3322678059426565 1104 2 -0.307196409672418 1104 3 -0.3139221733039328 1104 4 -0.19021579608968864 1104 5 -0.2292564087071014 1104 6 -0.3145264342095473 1104 7 -0.24230847190784033 1104 8 -0.30242148260851565 1104 9 -0.4610326067447801 1104 10-11 -0.4003053801299714 1104 12-13 -0.48039839274539986 1104 14-15 -0.5928455165744708 1104 16-17 -0.6388871178316649 1104 18-19 -0.6679576073292921 1104 20-21 -0.6953436480271051 1104 22-23 -0.7180214020732194 1104 24-25 -0.7657071169801384 1104 26-27 -0.8256178990112062 1104 28-29 -0.8510549968853738 1104 30-31 -0.7800071427272073 1104 32-33 -0.6881681536103557 1104 34-35 -0.6880712777675626 1104 36-37 -0.6940851653959683 1104 38-39 -0.816573851692084 1104 40-41 -0.8228669998385172 1104 42-43 -0.7312299694038487 1104 44-45 -0.8114800628452485 1104 46-47 -0.7085183899152554 1104 48-49 -0.7870847450695848 1104 50-51 -0.7607664278796378 1104 52-53 -0.7465816891448398 1104 54-55 -0.7834264933078003 1104 56-57 -0.7988935555228807 1104 58-59 -0.8593114091296314 1104 60-61 -0.8788963888555301 1104 62-63 -0.8880036926438706 1104 64-65 -0.7910228200590481 1104 66-67 -0.8491742149888992 1104 68-69 -0.8212023195984557 1104 70-71 -0.8865480682791329 1104 72-73 -0.7960250693083637 1104 74-75 -0.8375643551538516 1104 76-77 -0.6674992881146622 1104 78-79 -0.6591401102153256 1104 80-81 -0.800073188811961 1104 82-83 -0.7523426898598622 1104 84-85 -0.6654815782982659 1104 86-87 -0.5913754524704871 1104 88-89 -0.736990447129326 1104 90-91 -0.7182098369976408 1104 92-93 -0.865707347784145 1104 94-95 -0.8212875657440115 1104 96-97 -1.0603631125109025 1104 98-99 -0.9968173340725173 1104 100 -1.0277740413212868 1105 1 -0.3899396187559283 1105 2 -0.3966040374230211 1105 3 -0.4228712809313464 1105 4 -0.2624235951817582 1105 5 -0.254694753616171 1105 6 -0.33416157666288626 1105 7 -0.3499463323988081 1105 8 -0.3236124597050818 1105 9 -0.4877002838214537 1105 10-11 -0.506241472333997 1105 12-13 -0.5539320800810259 1105 14-15 -0.6977094949023837 1105 16-17 -0.6896432789310651 1105 18-19 -0.7041703169213704 1105 20-21 -0.7123556451701774 1105 22-23 -0.7713390046075546 1105 24-25 -0.8068610700154011 1105 26-27 -0.7863188527201928 1105 28-29 -0.7971062787229783 1105 30-31 -0.8020831834292537 1105 32-33 -0.7229202680929347 1105 34-35 -0.6931165859177284 1105 36-37 -0.8695603966357552 1105 38-39 -0.842160951406818 1105 40-41 -0.8224953877067875 1105 42-43 -0.7788534833459408 1105 44-45 -0.8352603023737544 1105 46-47 -0.8880223706181596 1105 48-49 -0.824431214543111 1105 50-51 -0.8513400346278388 1105 52-53 -0.8971616419159929 1105 54-55 -0.9379724355394288 1105 56-57 -0.8645937731256055 1105 58-59 -0.9407454494374647 1105 60-61 -1.0184837347492035 1105 62-63 -0.9913387458732643 1105 64-65 -0.9856148667318863 1105 66-67 -0.9337765358995114 1105 68-69 -1.0004972449675762 1105 70-71 -1.0144170163659325 1105 72-73 -1.0140436541394635 1105 74-75 -0.9916405377531063 1105 76-77 -0.9507222026162694 1105 78-79 -0.8596070457922558 1105 80-81 -0.7441446949374395 1105 82-83 -0.7801377321842367 1105 84-85 -0.753953072178458 1105 86-87 -0.7770940623129903 1105 88-89 -0.8274750064325787 1105 90-91 -0.7806780591401612 1105 92-93 -0.7866611968154658 1105 94-95 -0.8521468528991782 1105 96-97 -0.8941304273561634 1105 98-99 -0.946054720469391 1105 100 -0.9243494632195457 1106 1 -0.3419340820001331 1106 2 -0.2910659124362063 1106 3 -0.3440967118488274 1106 4 -0.17233443147299 1106 5 -0.19304702860455336 1106 6 -0.2977177564672502 1106 7 -0.3264949200496474 1106 8 -0.2914809450852829 1106 9 -0.45135609161268064 1106 10-11 -0.47166548904611716 1106 12-13 -0.49662396093997785 1106 14-15 -0.5999322648620904 1106 16-17 -0.6882001550181727 1106 18-19 -0.6838350829383657 1106 20-21 -0.6103782536546163 1106 22-23 -0.6083547075367122 1106 24-25 -0.6572798609891493 1106 26-27 -0.6730540764287198 1106 28-29 -0.7159709716163718 1106 30-31 -0.7189482619219163 1106 32-33 -0.6910018822266224 1106 34-35 -0.74606259940402 1106 36-37 -0.6953126895126545 1106 38-39 -0.7117148556856989 1106 40-41 -0.6980092719242634 1106 42-43 -0.6785830420624208 1106 44-45 -0.7698650553300297 1106 46-47 -0.7361249987432359 1106 48-49 -0.8105120785726712 1106 50-51 -0.7665898943833511 1106 52-53 -0.8903815469310032 1106 54-55 -0.8637215753853482 1106 56-57 -0.8289441034758767 1106 58-59 -0.8767967214503116 1106 60-61 -1.0269083315856307 1106 62-63 -0.7751968162884353 1106 64-65 -0.8480035220206688 1106 66-67 -0.8283733191734086 1106 68-69 -0.8861539470173909 1106 70-71 -0.8309301770838573 1106 72-73 -0.8712184749301386 1106 74-75 -0.8701410352529777 1106 76-77 -0.9146913585150891 1106 78-79 -0.7599497966790416 1106 80-81 -0.6949733228891901 1106 82-83 -0.7125753607509537 1106 84-85 -0.6719523930384028 1106 86-87 -0.720426491295072 1106 88-89 -0.7818599847807448 1106 90-91 -0.7139908277356817 1106 92-93 -0.6835582942959988 1106 94-95 -0.6898725374227617 1106 96-97 -0.8348477886571679 1106 98-99 -0.8788850099265417 1106 100 -0.9804232249981197 1107 1 -0.36934315416987573 1107 2 -0.3331775966259194 1107 3 -0.3974700151983015 1107 4 -0.2535815019622518 1107 5 -0.24792264934114172 1107 6 -0.31475970546262744 1107 7 -0.33700760475039004 1107 8 -0.39149856891307877 1107 9 -0.5106066660535618 1107 10-11 -0.4900121658594614 1107 12-13 -0.556476561529287 1107 14-15 -0.688956483647317 1107 16-17 -0.7208666306409981 1107 18-19 -0.702320158834759 1107 20-21 -0.7144696276581612 1107 22-23 -0.6896707905141355 1107 24-25 -0.68435893021433 1107 26-27 -0.6999435347609477 1107 28-29 -0.7277145898827584 1107 30-31 -0.7253529554785203 1107 32-33 -0.7318734366271471 1107 34-35 -0.7655456015928124 1107 36-37 -0.8108394926611666 1107 38-39 -0.8036430541298643 1107 40-41 -0.8334400561601143 1107 42-43 -0.750932514609346 1107 44-45 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2311 22-23 0.873199744230881 2311 24-25 0.8345154214011785 2311 26-27 0.8669146444166742 2311 28-29 0.8357049392847316 2311 30-31 0.9258153681453933 2311 32-33 1.0318537045696914 2311 34-35 0.9632805282793058 2311 36-37 1.0138475586239295 2311 38-39 1.085829170474355 2311 40-41 1.0268500770836937 2311 42-43 1.1700568065460075 2311 44-45 1.131492856839138 2311 46-47 1.2132629674476334 2311 48-49 1.1556564666646807 2311 50-51 1.1976111083960888 2311 52-53 1.2003579672674434 2311 54-55 1.1527378011186826 2311 56-57 1.1838873829273808 2311 58-59 1.2859839518290173 2311 60-61 1.3012768377449007 2311 62-63 1.2607838467938208 2311 64-65 1.3095618777160425 2311 66-67 1.2653861455162243 2311 68-69 1.2751966077824193 2311 70-71 1.288290359399916 2311 72-73 1.3045171255315253 2311 74-75 1.1593318009122058 2311 76-77 1.1195145525338788 2311 78-79 1.150549644257353 2311 80-81 1.124281885767381 2311 82-83 1.08300616318909 2311 84-85 1.1320518242256306 2311 86-87 1.1832530890688204 2311 88-89 1.1994404040171673 2311 90-91 1.2714895135610327 2311 92-93 1.2945920783132578 2311 94-95 1.3246768486142564 2311 96-97 1.3861374405838234 2311 98-99 1.3885178583027198 2311 100 1.554283900875383 2312 1 0.11575714235899426 2312 2 0.12278098113716851 2312 3 0.28221123921979796 2312 4 0.23082695507778794 2312 5 0.30289615591375707 2312 6 0.37724099225236074 2312 7 0.4216212016979668 2312 8 0.26040481592263376 2312 9 0.3592166100855039 2312 10-11 0.28025434290328377 2312 12-13 0.42277963757653225 2312 14-15 0.7237108990952521 2312 16-17 0.8077156308735951 2312 18-19 0.8196982571686249 2312 20-21 0.8521371824072972 2312 22-23 0.49960073996040677 2312 24-25 0.3897543750267971 2312 26-27 0.7606289587438724 2312 28-29 0.9027505840410583 2312 30-31 0.829042189894075 2312 32-33 0.6683294515456666 2312 34-35 0.8062654278121997 2312 36-37 0.8688033874328482 2312 38-39 0.879293407904342 2312 40-41 0.9231708851124836 2312 42-43 0.808722300083069 2312 44-45 0.741458449806089 2312 46-47 0.8788327579295441 2312 48-49 0.9497556247295904 2312 50-51 0.9274214762866748 2312 52-53 0.9196208985177563 2312 54-55 0.9597805845832781 2312 56-57 0.9469900246149763 2312 58-59 0.9760848838947922 2312 60-61 1.0062556284199502 2312 62-63 0.9852865185249655 2312 64-65 1.0206252052552571 2312 66-67 1.0165692181143484 2312 68-69 0.9369305505708923 2312 70-71 0.831705883338195 2312 72-73 0.8871044485785191 2312 74-75 0.9732587092149672 2312 76-77 0.9252374221233595 2312 78-79 0.8645788437394515 2312 80-81 0.725535703803537 2312 82-83 0.7402088213291123 2312 84-85 0.5866592907664092 2312 86-87 0.7526325464248416 2312 88-89 0.44717962588887517 2312 90-91 0.7886943176698473 2312 92-93 0.9627539129735183 2312 94-95 1.038329359040084 2312 96-97 1.0962951692904461 2312 98-99 1.0953531981168823 2312 100 1.2155553158556742 2313 1 -0.38897882373287374 2313 2 -0.04724391108715764 2313 3 0.20593759119469013 2313 4 0.030037457108058163 2313 5 0.19392227420991048 2313 6 0.3249275150203772 2313 7 0.12698525768858104 2313 8 0.0776762128534969 2313 9 0.29535875201980133 2313 10-11 0.36398471174655356 2313 12-13 0.5541998580387499 2313 14-15 0.560771602010135 2313 16-17 0.77387946389711 2313 18-19 0.6570682548285376 2313 20-21 0.8305218533784 2313 22-23 0.8379444429939511 2313 24-25 0.6918267577589603 2313 26-27 0.8983864667858512 2313 28-29 0.7267361455621781 2313 30-31 0.7423716217152503 2313 32-33 0.8831444860866142 2313 34-35 0.9268343605694085 2313 36-37 0.7710420108376326 2313 38-39 0.6100986107994544 2313 40-41 0.6888420193607558 2313 42-43 0.7743796039445314 2313 44-45 0.9007019354755457 2313 46-47 0.9289183398852003 2313 48-49 1.02770284843497 2313 50-51 0.9144486805792411 2313 52-53 0.9026485378680675 2313 54-55 0.9646385769565811 2313 56-57 1.031709066301417 2313 58-59 0.9492711215681595 2313 60-61 1.008432713401227 2313 62-63 1.0002893365126297 2313 64-65 1.0526839800733256 2313 66-67 1.0676449231056537 2313 68-69 1.1459012311396322 2313 70-71 1.062188673838115 2313 72-73 1.1262794744512732 2313 74-75 0.8328638325142848 2313 76-77 0.9545283521581638 2313 78-79 0.7259538404435588 2313 80-81 0.8508945193258697 2313 82-83 0.9074524874486585 2313 84-85 0.9072933585892748 2313 86-87 1.0195345480200544 2313 88-89 0.9657613500029427 2313 90-91 1.1235113132665369 2313 92-93 0.7933327704855131 2313 94-95 0.8216565084560514 2313 96-97 1.1305863341575595 2313 98-99 1.2585517129879449 2313 100 1.1986866851669653 2314 1 -0.3492354766125345 2314 2 -0.1960867538762976 2314 3 0.07033097928584198 2314 4 -0.17363879542823213 2314 5 0.16379192361681305 2314 6 0.18657913466815756 2314 7 0.093982674251194 2314 8 0.1968884026459108 2314 9 0.19147398730932252 2314 10-11 0.39043681410205267 2314 12-13 0.4574759501060228 2314 14-15 0.5101136749706967 2314 16-17 0.7060379307107567 2314 18-19 0.7857972694804829 2314 20-21 0.40927486522090817 2314 22-23 0.6644612613501693 2314 24-25 0.8374163391412992 2314 26-27 0.7983355134015326 2314 28-29 0.7255301119596069 2314 30-31 0.7358427803763519 2314 32-33 0.8497423478003654 2314 34-35 0.9068053842869617 2314 36-37 0.8386656589633859 2314 38-39 0.8000695572902714 2314 40-41 0.8513499777786322 2314 42-43 0.6984007307394435 2314 44-45 0.7384667743887121 2314 46-47 0.8544805382944958 2314 48-49 0.7868159969769266 2314 50-51 0.9345523107233618 2314 52-53 1.0177246590376328 2314 54-55 1.001933409919225 2314 56-57 0.8838918188897367 2314 58-59 0.9274489283940284 2314 60-61 1.0279226755519986 2314 62-63 0.7094941613136356 2314 64-65 0.8472316062049003 2314 66-67 0.921821466664781 2314 68-69 0.9803236930627861 2314 70-71 1.0586610949530453 2314 72-73 1.0180332406770063 2314 74-75 1.0633285626596987 2314 76-77 0.7677631035368648 2314 78-79 0.7974416174842958 2314 80-81 0.85946134564864 2314 82-83 0.8501167487058794 2314 84-85 0.6978187970745218 2314 86-87 0.839593541113544 2314 88-89 0.9495765578160089 2314 90-91 1.0523720482699197 2314 92-93 1.1072239246918514 2314 94-95 1.1406544591024819 2314 96-97 1.1883884898401469 2314 98-99 1.258119942398487 2314 100 1.250057134480219 2315 1 -0.0756271784375997 2315 2 0.08362302879960737 2315 3 0.09527739740997987 2315 4 -0.08720219232414905 2315 5 0.1958002332073221 2315 6 0.3077447921658987 2315 7 0.39345334017584577 2315 8 0.33325839108763944 2315 9 0.5551776531372639 2315 10-11 0.5484249886716341 2315 12-13 0.5662156344652516 2315 14-15 0.6264978813580342 2315 16-17 0.6900775475277996 2315 18-19 0.7594065903010758 2315 20-21 0.7580269640227542 2315 22-23 0.8212530514874246 2315 24-25 0.7304000188264865 2315 26-27 0.8276818803141097 2315 28-29 0.8798454942943152 2315 30-31 0.8709649429310318 2315 32-33 0.8889965537279068 2315 34-35 0.8664667894813647 2315 36-37 0.8545761624062891 2315 38-39 0.8979646354314994 2315 40-41 0.8981700333661564 2315 42-43 0.9334105528942231 2315 44-45 0.7813396753886863 2315 46-47 0.8211037345933079 2315 48-49 0.9275570424854465 2315 50-51 0.8392491993437687 2315 52-53 0.8739776813915512 2315 54-55 0.8528013189814558 2315 56-57 0.9326909435367554 2315 58-59 0.9071758423665344 2315 60-61 0.9889812327178973 2315 62-63 0.9405684505749008 2315 64-65 0.9826567889730811 2315 66-67 0.9854066205451986 2315 68-69 0.9271056808639742 2315 70-71 0.5450628646849509 2315 72-73 0.8164199418440958 2315 74-75 1.0120128735312974 2315 76-77 0.9416666926267396 2315 78-79 0.8276281076217131 2315 80-81 0.8501198105385939 2315 82-83 0.8658169280256018 2315 84-85 0.7322174457553068 2315 86-87 0.7704830882479996 2315 88-89 0.8601297456958363 2315 90-91 0.9261071703611314 2315 92-93 0.9718777472461824 2315 94-95 1.0195454157438881 2315 96-97 1.1356446681261296 2315 98-99 1.0027448415212277 2315 100 0.939517462963515 2316 1 0.5082965131772283 2316 2 0.4746532280972886 2316 3 0.4719174457536397 2316 4 0.290531315261525 2316 5 0.3992219671688204 2316 6 0.42821635267029023 2316 7 0.4769346456044943 2316 8 0.46308835391308634 2316 9 0.5137811791865587 2316 10-11 0.4756302589846726 2316 12-13 0.6385179218457111 2316 14-15 0.7594511056466757 2316 16-17 0.764968185990341 2316 18-19 0.8030777292723528 2316 20-21 0.8583399755913064 2316 22-23 0.8795564875044093 2316 24-25 0.9239416332928059 2316 26-27 0.9500228323784015 2316 28-29 0.9244083067000943 2316 30-31 0.8206991052744925 2316 32-33 0.8067244018181015 2316 34-35 0.6954323802235294 2316 36-37 0.7122548711869641 2316 38-39 0.6379848252538025 2316 40-41 0.6974351545613047 2316 42-43 0.8478784141795117 2316 44-45 0.7905337481195147 2316 46-47 0.7669187962457045 2316 48-49 0.5065658351255848 2316 50-51 0.6029842334069144 2316 52-53 0.7115933319512422 2316 54-55 0.8122163599743857 2316 56-57 0.8674908171067983 2316 58-59 0.7731550300122265 2316 60-61 0.8090542116060178 2316 62-63 0.8855007071260346 2316 64-65 0.8721711278188522 2316 66-67 0.8065246419476111 2316 68-69 0.8280447749000359 2316 70-71 0.6729169691119452 2316 72-73 0.7540911412699316 2316 74-75 0.8281996020331448 2316 76-77 0.689578574869266 2316 78-79 0.6718749919014542 2316 80-81 0.6579425610359166 2316 82-83 0.6026650024856295 2316 84-85 0.6399500421509678 2316 86-87 0.6579826136048084 2316 88-89 0.6268231963942696 2316 90-91 0.7177880555057179 2316 92-93 0.7832307188997447 2316 94-95 0.6951464233114528 2316 96-97 0.811789907192658 2316 98-99 0.913532984659625 2316 100 0.63942805517582 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 7.0 12 42.0 13 243.0 14 885.0 15 2650.0 16 6607.0 17 13435.0 18 21864.0 19 30607.0 20 41671.0 21 55285.0 22 67065.0 23 71797.0 24 74735.0 25 82659.0 26 97577.0 27 119329.0 28 146468.0 29 178789.0 30 217997.0 31 270107.0 32 339363.0 33 440927.0 34 617051.0 35 837403.0 36 1268785.0 37 2008234.0 38 1898286.0 39 306782.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25771593295444 19.358561085633923 13.036041791391503 24.347681190020133 2 15.703109708437438 20.896409164076797 40.25975068690648 23.14073044057928 3 17.989083709498992 26.944734136485533 30.784824563801905 24.281357590213574 4 12.167030200431899 17.1945176937852 38.44391270848932 32.19453939729357 5 12.541571721670113 37.84125540170618 34.478908620714535 15.138264255909176 6 29.61134716253125 37.277988187290376 19.306359999579023 13.804304650599352 7 26.757174390061834 33.07566982963048 22.9109813173345 17.25617446297319 8 26.24848792657722 35.37523236482153 20.6148351749101 17.76144453369115 9 27.485631762999223 15.563656959087552 20.37954713877596 36.57116413913727 10-11 25.019471648211482 25.863800940595755 29.079719208986432 20.037008202206334 12-13 25.32987011127027 23.949282917951386 28.525100963928573 22.19574600684977 14-15 22.821458180010787 25.48161860001447 26.51766400507948 25.179259214895254 16-17 22.157870881345257 27.728625635430987 27.274410979444596 22.839092503779153 18-19 21.84523197280063 27.433114773165705 28.840882469226457 21.880770784807204 20-21 22.902527791043276 26.981435350362197 28.274302790152227 21.8417340684423 22-23 22.543613437523376 26.713746870010237 28.278056852011897 22.464582840454487 24-25 22.089257332116556 26.45902096818626 28.69909078944613 22.75263091025106 26-27 22.1778377318608 26.631544124930844 28.541477449099638 22.649140694108716 28-29 21.97384294166354 27.59546381258284 28.3529019314021 22.077791314351522 30-31 23.254133087747363 26.68015393101748 28.44963585254252 21.61607712869263 32-33 22.884956119047835 26.825945906998168 28.193359845510575 22.095738128443426 34-35 22.93958024368064 26.930812381122248 28.447439245745905 21.68216812945121 36-37 22.50115804956235 26.864101635726012 28.48059556090407 22.15414475380756 38-39 22.374461978356937 27.03881200393542 28.427760278109794 22.15896573959785 40-41 22.515277619022537 26.407231636897865 28.818238390585492 22.25925235349411 42-43 22.28482597422184 26.682547566069527 28.223747080311473 22.808879379397165 44-45 21.711035917665402 27.06211532375618 28.648670262561993 22.578178496016427 46-47 21.73440746613376 26.392054493296634 28.371453697148635 23.50208434342097 48-49 21.94884127142062 26.572156421875054 28.540103224991615 22.938899081712712 50-51 21.85636266766207 26.867322213915152 28.322413574211865 22.953901544210918 52-53 21.68032718617642 27.15573209274283 27.94414304265195 23.219797678428797 54-55 21.463359267706743 26.684848560805314 28.500140764276505 23.35165140721144 56-57 21.956440185614625 26.599778709871497 27.959001378791033 23.48477972572285 58-59 21.46447412545398 26.78938525848016 28.346604717085395 23.399535898980474 60-61 22.012357486180925 26.98457123478893 27.883289118336823 23.119782160693326 62-63 21.90459047486678 26.90760378927338 27.685369288475336 23.502436447384504 64-65 21.418356225318185 26.57687302632968 27.738024254505454 24.266746493846682 66-67 21.462262192460855 26.585201323141998 27.482604955209872 24.46993152918727 68-69 21.112329620125532 26.78865350749303 27.676195354086392 24.42282151829504 70-71 21.397951612168747 26.439678201822446 27.794733304737107 24.367636881271697 72-73 21.476852707385106 26.97157600441338 27.197829348568487 24.353741939633032 74-75 21.74197604966212 26.968980818316684 26.88450633053993 24.404536801481264 76-77 21.492542934353175 26.7997665937937 26.970680800841805 24.737009671011315 78-79 21.21391939229645 26.957512880951384 27.182350345817817 24.646217380934345 80-81 21.313474056586884 27.178059914835185 26.774321264881763 24.734144763696168 82-83 21.69030310479395 27.018851358372793 26.8934777105526 24.397367826280657 84-85 21.77298009160334 27.248051053066096 26.400247671068595 24.57872118426197 86-87 21.194065283960626 27.39250764325987 26.66485855742517 24.74856851535434 88-89 21.23556313144294 26.994769514657623 26.698876371437034 25.0707909824624 90-91 21.751325753032823 26.669405780227866 26.6684994957911 24.91076897094821 92-93 21.672871133770837 26.857068189737042 26.51808033789606 24.951980338596062 94-95 21.189268844956388 26.6884942687218 26.666689814797234 25.455547071524578 96-97 21.096105068783114 26.68841575619435 26.84628780160681 25.369191373415727 98-99 21.025123565658134 26.801738386721862 26.6132827500339 25.559855297586104 100 20.931429809174436 26.90095223956658 26.657726805302882 25.509891145956097 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 805.0 1 610.0 2 920.0 3 1440.5 4 1163.0 5 950.0 6 1312.5 7 2332.5 8 3647.0 9 4454.5 10 4429.5 11 4326.0 12 4810.0 13 5546.0 14 6464.0 15 7384.0 16 8307.0 17 9303.0 18 10404.0 19 11582.5 20 12965.5 21 14820.5 22 17377.0 23 21004.0 24 25683.5 25 31402.0 26 39319.5 27 49283.0 28 60773.0 29 76059.5 30 94289.0 31 111566.0 32 132158.0 33 153306.5 34 172341.5 35 191786.5 36 212129.0 37 237601.5 38 254844.5 39 259919.0 40 263637.5 41 268661.5 42 277852.0 43 291195.5 44 308655.0 45 332560.5 46 352736.0 47 370714.5 48 392849.5 49 407843.0 50 414895.0 51 418550.0 52 407288.0 53 381226.5 54 352799.0 55 315797.5 56 276316.0 57 238538.5 58 202560.5 59 169731.0 60 132483.5 61 99093.0 62 75975.5 63 57950.5 64 41563.0 65 28659.5 66 20505.5 67 15366.5 68 11524.0 69 8453.0 70 6309.5 71 4645.0 72 3419.0 73 2549.0 74 1823.0 75 1244.0 76 868.5 77 548.5 78 320.5 79 187.5 80 111.5 81 74.0 82 58.5 83 41.0 84 26.0 85 17.0 86 12.5 87 8.5 88 6.5 89 3.5 90 2.0 91 2.0 92 3.0 93 3.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05141762482038008 2 0.0 3 0.0 4 0.017186224459903365 5 0.004155507555645826 6 1.0849889179231923E-5 7 1.3019867015078308E-4 8 3.7974612127311734E-4 9 0.0018878807171863546 10-11 8.517163005697059E-4 12-13 2.8209711866002997E-4 14-15 3.254966753769577E-5 16-17 7.052427966500749E-5 18-19 0.0 20-21 5.424944589615962E-6 22-23 5.424944589615962E-6 24-25 7.594922425462346E-5 26-27 1.0849889179231923E-5 28-29 9.710650815412572E-4 30-31 9.547902477724093E-4 32-33 0.014061456376284575 34-35 0.02303973967209899 36-37 0.03163827684664029 38-39 0.020679888775616044 40-41 1.681732822780948E-4 42-43 0.0017522571024459555 44-45 0.009748625427539882 46-47 0.014235054603152284 48-49 0.005479194035512122 50-51 0.010567792060571894 52-53 0.016301958491795963 54-55 0.01000902276784145 56-57 0.0029999943580576267 58-59 0.008707036066333619 60-61 0.0017956566591628834 62-63 9.602151923620252E-4 64-65 0.019285678016084745 66-67 0.04577025750258987 68-69 0.054922139025271995 70-71 0.04896012492128406 72-73 0.04102885593126552 74-75 0.0288552802721673 76-77 0.02934352528523274 78-79 0.01234717388596593 80-81 0.002191677614204849 82-83 0.028497233929252644 84-85 0.04845018012986015 86-87 0.0486346282459071 88-89 0.0606346056781376 90-91 0.03516449082989066 92-93 0.027010799111697875 94-95 0.007345374974340012 96-97 0.0039005351599338766 98-99 0.022225997983656594 100 0.019269403182315896 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9216684.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.971226320523755 #Duplication Level Percentage of deduplicated Percentage of total 1 76.46331077308804 47.385251371355814 2 14.666760931581365 18.178343221600894 3 4.3699485299596725 8.124332080775128 4 1.7171487523980746 4.2565525584346435 5 0.8498071031838799 2.6331794160098445 6 0.47618056275075943 1.7705696054197004 7 0.30028577641572074 1.3026354467764978 8 0.20855131899929713 1.033934479131935 9 0.14931796937140412 0.8328075906272686 >10 0.7371040461122097 8.031208065342101 >50 0.040487968673696015 1.7050707877688542 >100 0.01830346033224857 2.1151799423116633 >500 0.0018619899686165252 0.7897062455561817 >1k 8.009357059837298E-4 0.8608849639393354 >5k 6.494072956751981E-5 0.23610145676765695 >10k+ 6.494072956751981E-5 0.7442427681825478 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23721 0.2573702212205604 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19252 0.208882066478573 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12506 0.13568871407547445 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0849889179231923E-5 2 0.0 0.0 0.0 0.0 1.0849889179231923E-5 3 0.0 0.0 0.0 1.0849889179231923E-5 2.1699778358463847E-5 4 0.0 0.0 0.0 5.424944589615962E-5 2.1699778358463847E-5 5 0.0 1.0849889179231923E-5 0.0 5.424944589615962E-5 2.1699778358463847E-5 6 0.0 1.0849889179231923E-5 0.0 5.424944589615962E-5 4.3399556716927694E-5 7 0.0 1.0849889179231923E-5 0.0 5.424944589615962E-5 4.3399556716927694E-5 8 0.0 2.1699778358463847E-5 0.0 5.424944589615962E-5 5.424944589615962E-5 9 0.0 2.1699778358463847E-5 0.0 7.594922425462346E-5 7.594922425462346E-5 10-11 0.0 2.1699778358463847E-5 0.0 7.594922425462346E-5 7.594922425462346E-5 12-13 0.0 2.1699778358463847E-5 0.0 9.76490026130873E-5 9.222405802347135E-5 14-15 0.0 2.712472294807981E-5 0.0 1.1934878097155115E-4 1.0849889179231923E-4 16-17 0.0 3.254966753769577E-5 0.0 1.4104855933001498E-4 1.0849889179231923E-4 18-19 0.0 3.797461212731173E-5 0.0 2.224227281742544E-4 1.5189844850924691E-4 20-21 0.0 5.424944589615962E-5 0.0 3.688962320938854E-4 1.5732339309886288E-4 22-23 0.0 5.424944589615962E-5 0.0 0.001399635704120918 1.8987306063655867E-4 24-25 0.0 8.137416884423942E-5 0.0 0.003406865202278824 2.386975619431023E-4 26-27 0.0 8.679911343385539E-5 0.0 0.0068191553491472635 2.4412250653271827E-4 28-29 0.0 9.222405802347135E-5 0.0 0.019090380010858568 2.4954745112233423E-4 30-31 0.0 1.1934878097155115E-4 0.0 0.06553333064256081 2.549723957119502E-4 32-33 0.0 1.1934878097155115E-4 0.0 0.17182969493149597 2.9294700783926195E-4 34-35 0.0 1.2477372556116712E-4 0.0 0.3228276026388667 2.9294700783926195E-4 36-37 0.0 1.4104855933001498E-4 0.0 0.5563606173326546 2.983719524288779E-4 38-39 0.0 1.6274833768847884E-4 0.0 0.9423996743297265 3.0379689701849383E-4 40-41 0.0 1.7902317145732674E-4 0.0 1.4023156267481882 3.0379689701849383E-4 42-43 0.0 2.1699778358463847E-4 0.0 1.839734333953513 3.0379689701849383E-4 44-45 0.0 2.1699778358463847E-4 0.0 2.310923321229197 3.092218416081098E-4 46-47 0.0 2.1699778358463847E-4 0.0 2.838602256516552 3.200717307873417E-4 48-49 0.0 2.2242272817425443E-4 0.0 3.4016301307498447 3.688962320938854E-4 50-51 0.0 2.278476727638704E-4 0.0 4.001097357791588 3.688962320938854E-4 52-53 0.0 2.278476727638704E-4 0.0 4.599533845361304 3.960209550419652E-4 54-55 0.0 2.278476727638704E-4 0.0 5.25954887896775 4.3399556716927694E-4 56-57 0.0 2.278476727638704E-4 0.0 5.939625357666596 4.5027040093812483E-4 58-59 0.0 2.3327261735348634E-4 0.0 6.6841935776467984 4.665452347069727E-4 60-61 0.0 2.4412250653271827E-4 0.0 7.4220402912804655 4.773951238862046E-4 62-63 0.0 2.4954745112233423E-4 0.0 8.204762146559435 4.773951238862046E-4 64-65 0.0 2.4954745112233423E-4 0.0 8.916634225498022 4.773951238862046E-4 66-67 0.0 2.8209711866003E-4 0.0 9.681746710639096 4.773951238862046E-4 68-69 0.0 2.8209711866003E-4 0.0 10.531043485921835 4.773951238862046E-4 70-71 0.0 2.8209711866003E-4 0.0 11.419242538856707 4.773951238862046E-4 72-73 0.0 2.8209711866003E-4 0.0 12.226767240799402 5.58769292730444E-4 74-75 1.0849889179231923E-5 2.8209711866003E-4 0.0 13.005783858923664 5.6419423732006E-4 76-77 2.1699778358463847E-5 2.87522063249646E-4 0.0 13.831080679341941 5.6419423732006E-4 78-79 2.1699778358463847E-5 3.0379689701849383E-4 0.0 14.718145918857584 5.696191819096761E-4 80-81 2.1699778358463847E-5 3.0379689701849383E-4 0.0 15.590737406208133 5.913189602681398E-4 82-83 2.1699778358463847E-5 3.092218416081098E-4 0.0 16.44766707852846 6.401434615746834E-4 84-85 2.1699778358463847E-5 3.1464678619772576E-4 0.0 17.283200769387342 6.455684061642995E-4 86-87 2.1699778358463847E-5 3.1464678619772576E-4 0.0 18.129709123151017 6.564182953435313E-4 88 2.1699778358463847E-5 3.1464678619772576E-4 0.0 18.768897794477926 6.726931291123792E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 10075 0.0 52.005245 2 ACCTGGG 11475 0.0 44.964943 3 GGTATCA 16670 0.0 41.205566 1 GTATCAA 27755 0.0 36.31824 1 CCTGGGG 10970 0.0 34.227036 4 GTACCTG 17220 0.0 30.852226 1 ATCAACG 32930 0.0 29.95333 3 CAACGCA 33220 0.0 29.67464 5 TGGGGGG 18910 0.0 29.094734 6 TATCAAC 34280 0.0 29.08854 2 CTGGGGG 7920 0.0 29.01104 5 GTACATG 31870 0.0 28.914473 1 TCAACGC 34210 0.0 28.887856 4 AACGCAG 35345 0.0 28.539942 6 TACATGG 32345 0.0 27.995733 2 ACATGGG 31875 0.0 27.11165 3 CATGGGG 23640 0.0 26.418406 4 ATGGGGG 18050 0.0 25.84947 5 TAACGCA 1070 0.0 25.472603 4 ACGCAGA 39370 0.0 25.216398 7 >>END_MODULE