FastQCFastQC Report
Mon 6 Jun 2016
ERR1211405_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211405_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10682567
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT307970.288292130533794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231880.2170639322926783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153690.14386991441289346No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC109300.10231623166978498No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG106890.10006021960826457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG118100.055.07462
GGTATCA170900.047.0572781
ACCTGGG136900.046.5966453
GTATCAA308950.040.456141
CCTGGGG126250.036.7128144
GTACATG465850.033.5961041
TACATGG470050.032.84152
ATCAACG375800.032.723463
CAACGCA377500.032.7126165
TATCAAC386950.032.085822
CTGGGGG99300.031.8594265
TGGGGGG316050.031.8446946
GTACCTG210100.031.4381751
ACATGGG469600.031.4325643
TCAACGC396800.031.0504234
CATGGGG355900.030.9072274
AACGCAG404100.030.8155086
ATGGGGG286350.030.5507135
ACGCAGA446700.027.393067
CGCAGAG450750.026.989998
Produced by FastQC (version 0.11.5)