##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211405_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 10682567 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.926382675624687 31.0 31.0 34.0 27.0 34.0 2 30.964859850633278 31.0 31.0 34.0 27.0 34.0 3 31.019612795314085 31.0 31.0 34.0 27.0 34.0 4 34.649029020833666 37.0 35.0 37.0 31.0 37.0 5 34.70450978683307 37.0 35.0 37.0 31.0 37.0 6 34.764595812972665 37.0 35.0 37.0 31.0 37.0 7 34.67972211173588 37.0 35.0 37.0 30.0 37.0 8 34.67389205235034 37.0 35.0 37.0 30.0 37.0 9 36.17271906649404 39.0 35.0 39.0 30.0 39.0 10-11 36.07145342500543 39.0 35.0 39.0 30.0 39.0 12-13 35.95672505494232 39.0 35.0 39.0 30.0 39.0 14-15 37.05939714677193 40.0 36.0 41.0 30.5 41.0 16-17 37.03495568995729 40.0 36.0 41.0 30.0 41.0 18-19 37.02921137775218 40.0 36.0 41.0 30.0 41.0 20-21 37.01514032160996 40.0 36.0 41.0 30.0 41.0 22-23 36.891649544533635 39.5 36.0 41.0 30.0 41.0 24-25 36.79320050134018 39.0 36.0 41.0 30.0 41.0 26-27 36.648324789350724 39.0 36.0 41.0 29.5 41.0 28-29 36.4988951157526 39.0 35.0 41.0 29.0 41.0 30-31 36.337616323866726 39.0 35.0 40.5 29.0 41.0 32-33 36.12500029253269 39.0 35.0 40.0 28.0 41.0 34-35 36.01175401006144 38.5 35.0 40.0 28.0 41.0 36-37 35.898684932189056 38.0 35.0 40.0 27.0 41.0 38-39 35.744479533804935 38.0 34.5 40.0 27.0 41.0 40-41 35.58084943441028 38.0 34.0 40.0 26.5 41.0 42-43 35.392484690243464 38.0 34.0 40.0 26.0 41.0 44-45 35.26472115737725 38.0 34.0 40.0 25.0 41.0 46-47 35.052072034745954 38.0 33.0 40.0 24.5 41.0 48-49 34.81266904293696 38.0 33.0 40.0 24.0 41.0 50-51 34.17383794550504 37.5 32.5 39.5 23.5 40.5 52-53 34.51621553134186 37.5 33.0 39.5 24.0 40.5 54-55 34.93837810705985 38.0 33.5 40.0 24.5 41.0 56-57 34.86878074342992 38.0 33.0 40.0 24.5 41.0 58-59 34.6755231677929 38.0 33.0 40.0 24.0 41.0 60-61 34.45305341871481 37.0 33.0 40.0 24.0 41.0 62-63 34.14498869981344 37.0 32.5 40.0 23.0 41.0 64-65 33.936327429540114 36.0 32.0 40.0 23.0 41.0 66-67 33.744396828964426 36.0 32.0 39.0 23.0 41.0 68-69 33.40675111141358 35.5 32.0 39.0 22.0 41.0 70-71 33.029396632850506 35.0 31.5 39.0 22.0 40.5 72-73 32.64243224498381 35.0 31.0 37.5 21.0 40.0 74-75 32.27761098994277 35.0 31.0 37.0 20.0 39.5 76-77 31.91187033977882 35.0 30.5 36.5 20.0 39.0 78-79 31.540983548242664 35.0 30.0 36.0 19.5 39.0 80-81 31.192909578755742 34.0 30.0 36.0 18.5 37.5 82-83 30.857320108546944 34.0 29.5 35.0 18.0 37.0 84-85 30.544903205381253 34.0 29.0 35.0 18.0 37.0 86-87 30.23274279487318 34.0 29.0 35.0 17.0 36.0 88-89 29.965856287163938 34.0 29.0 35.0 15.5 36.0 90-91 29.697282310515817 34.0 28.5 35.0 14.5 36.0 92-93 29.43467674951161 34.0 27.5 35.0 11.5 35.0 94-95 29.17900028148665 34.0 27.0 35.0 10.5 35.0 96-97 28.913608592391697 34.0 26.5 35.0 10.0 35.0 98-99 28.626291274372537 33.0 25.5 35.0 10.0 35.0 100 27.371228469711447 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.26439076405652173 1101 2 -0.2870718055842012 1101 3 -0.3514467592771453 1101 4 -0.21428087725165312 1101 5 -0.2574262855857157 1101 6 -0.2168977113311925 1101 7 -0.26617260015384403 1101 8 -0.23896342666095904 1101 9 -0.4051842630167073 1101 10-11 -0.3970956461621711 1101 12-13 -0.39775746611186236 1101 14-15 -0.6109286659283555 1101 16-17 -0.5850277559805406 1101 18-19 -0.6023555323274152 1101 20-21 -0.6005772260241287 1101 22-23 -0.5476421857411609 1101 24-25 -0.6221277248173678 1101 26-27 -0.654188810597482 1101 28-29 -0.6965105916889911 1101 30-31 -0.7598668763137439 1101 32-33 -0.7112655202936935 1101 34-35 -0.7074353245966378 1101 36-37 -0.7298299628150673 1101 38-39 -0.767190851610259 1101 40-41 -0.9119924973949978 1101 42-43 -0.9603913468732088 1101 44-45 -0.9590846446984926 1101 46-47 -0.9611702084398317 1101 48-49 -0.9914094111285792 1101 50-51 -0.9605895587576683 1101 52-53 -0.7460031061526138 1101 54-55 -0.6730181915625693 1101 56-57 -0.7696128521431973 1101 58-59 -0.7903281204412735 1101 60-61 -0.767369915165176 1101 62-63 -0.8978074728250007 1101 64-65 -0.8096415487249686 1101 66-67 -0.9120377835288878 1101 68-69 -0.874362217190999 1101 70-71 -0.8756106938309287 1101 72-73 -1.0391882774052306 1101 74-75 -1.1624108865141736 1101 76-77 -1.073400039056171 1101 78-79 -1.1306542322050035 1101 80-81 -1.2340945531206167 1101 82-83 -1.2999393626700275 1101 84-85 -1.3274506213264026 1101 86-87 -1.4428524191811718 1101 88-89 -1.5012521607886953 1101 90-91 -1.6308130871137898 1101 92-93 -1.7833885425315295 1101 94-95 -1.8202268339728072 1101 96-97 -1.8346243191017813 1101 98-99 -1.993805718988746 1101 100 -2.050176308176752 1102 1 -0.38756704012833865 1102 2 -0.39361136133496544 1102 3 -0.4138602977647352 1102 4 -0.43528641517930566 1102 5 -0.29271529649255257 1102 6 -0.26259955713887706 1102 7 -0.4419096770720117 1102 8 -0.3641677574681026 1102 9 -0.546340929229217 1102 10-11 -0.4577190393600148 1102 12-13 -0.5645606591479222 1102 14-15 -0.7538843738446985 1102 16-17 -0.7518287535847676 1102 18-19 -0.7243397216792786 1102 20-21 -0.8152069513716782 1102 22-23 -0.7335925426364582 1102 24-25 -0.8389178370117989 1102 26-27 -0.8252618072642903 1102 28-29 -0.9231001121916123 1102 30-31 -0.8343678399117138 1102 32-33 -0.9355773785681478 1102 34-35 -0.9317251138659799 1102 36-37 -1.020193829100286 1102 38-39 -1.1118694557900852 1102 40-41 -1.2567103244391475 1102 42-43 -1.2371913765213662 1102 44-45 -1.195502900546515 1102 46-47 -1.4456868926404738 1102 48-49 -1.429106315969456 1102 50-51 -1.1935155051378814 1102 52-53 -1.0460139948391713 1102 54-55 -1.0527071014926506 1102 56-57 -1.0339630629734629 1102 58-59 -1.0681770094577416 1102 60-61 -1.1452291948000806 1102 62-63 -1.0709470753548445 1102 64-65 -1.2340643811636482 1102 66-67 -1.1569225092673534 1102 68-69 -1.1315593670355142 1102 70-71 -1.209224010258783 1102 72-73 -1.1301876491185183 1102 74-75 -1.3129111959389945 1102 76-77 -1.2868537829785076 1102 78-79 -1.291117394923134 1102 80-81 -1.4152240202263933 1102 82-83 -1.5128561269023386 1102 84-85 -1.5426879897782548 1102 86-87 -1.5564173568109183 1102 88-89 -1.6796151052546904 1102 90-91 -1.6811983460800484 1102 92-93 -1.7247511959553528 1102 94-95 -1.8767380522690509 1102 96-97 -1.9736524977643164 1102 98-99 -2.012901617876196 1102 100 -2.0679516056626817 1103 1 -0.43022371669801984 1103 2 -0.5326812252335209 1103 3 -0.51131190642365 1103 4 -0.3300090016121189 1103 5 -0.25962699862839145 1103 6 -0.30002654691871555 1103 7 -0.3018598354398989 1103 8 -0.30053097444208987 1103 9 -0.47460435184000715 1103 10-11 -0.5480503130425234 1103 12-13 -0.5687629394762368 1103 14-15 -0.7482651926649666 1103 16-17 -0.7525188566951684 1103 18-19 -0.7315930557935886 1103 20-21 -0.6798479642799791 1103 22-23 -0.6930191880612782 1103 24-25 -0.6622710731326507 1103 26-27 -0.805991348787451 1103 28-29 -0.8728752382513747 1103 30-31 -0.846385342235422 1103 32-33 -0.9760818686173138 1103 34-35 -0.8940117159069061 1103 36-37 -0.8899353200711673 1103 38-39 -0.8962430592235435 1103 40-41 -0.9601071824630125 1103 42-43 -0.8540990225938643 1103 44-45 -0.9412173715697634 1103 46-47 -1.0973391056398611 1103 48-49 -1.031922878323158 1103 50-51 -0.9845214524757537 1103 52-53 -0.8250817145099916 1103 54-55 -0.8591676220873055 1103 56-57 -0.861139581554518 1103 58-59 -0.7020347347638562 1103 60-61 -0.7359388724117295 1103 62-63 -0.7495933834344086 1103 64-65 -0.7841543013667618 1103 66-67 -0.8611811079704665 1103 68-69 -0.8788112761282107 1103 70-71 -0.9271806346568638 1103 72-73 -0.9179329146040587 1103 74-75 -0.9819904643754853 1103 76-77 -0.9406226325502196 1103 78-79 -1.0327883509091897 1103 80-81 -0.9986352135616983 1103 82-83 -1.097785233912692 1103 84-85 -1.2232781089833225 1103 86-87 -1.296956049106722 1103 88-89 -1.3501644590793056 1103 90-91 -1.340343466584006 1103 92-93 -1.5615385868768357 1103 94-95 -1.4277359863980656 1103 96-97 -1.6341084200185243 1103 98-99 -1.678940631417401 1103 100 -1.6791414334877608 1104 1 -0.5401134276821082 1104 2 -0.5001231946812013 1104 3 -0.5149583340489379 1104 4 -0.35715574660703453 1104 5 -0.3896326610749554 1104 6 -0.42076160410917396 1104 7 -0.3592076641735815 1104 8 -0.4416263920005363 1104 9 -0.4764689672131581 1104 10-11 -0.4995578674726602 1104 12-13 -0.6280930901669137 1104 14-15 -0.8008795911455948 1104 16-17 -0.7619906172326054 1104 18-19 -0.7817544329805202 1104 20-21 -0.9070622577411811 1104 22-23 -0.8468543060502256 1104 24-25 -0.8629244769632223 1104 26-27 -0.7762496720886034 1104 28-29 -0.9362878346888621 1104 30-31 -1.0148863280412925 1104 32-33 -0.9281241191214775 1104 34-35 -0.9721418580293957 1104 36-37 -1.0076579692393395 1104 38-39 -1.06015776388098 1104 40-41 -0.9427585210415046 1104 42-43 -1.0231299949404118 1104 44-45 -1.0945331603215607 1104 46-47 -1.0626939369098736 1104 48-49 -1.1627409299792717 1104 50-51 -1.076976185287947 1104 52-53 -1.0144197924565077 1104 54-55 -0.9169313819890661 1104 56-57 -0.8641839932370701 1104 58-59 -0.8914122563750055 1104 60-61 -0.8498159494852615 1104 62-63 -0.9603225004745894 1104 64-65 -0.9218072723012511 1104 66-67 -0.9169281186973706 1104 68-69 -1.016388291285665 1104 70-71 -0.9118358955463819 1104 72-73 -0.9524990160900373 1104 74-75 -0.9897550772848618 1104 76-77 -1.1171711948450458 1104 78-79 -1.033234380757765 1104 80-81 -1.1599790845093914 1104 82-83 -1.1655023332397754 1104 84-85 -1.1600656835876215 1104 86-87 -1.262038711682564 1104 88-89 -1.2466992606468779 1104 90-91 -1.2255721708362337 1104 92-93 -1.3131498505227377 1104 94-95 -1.3826030361660422 1104 96-97 -1.4353648007482924 1104 98-99 -1.4914142527390872 1104 100 -1.473199107300868 1105 1 -0.5440969108120228 1105 2 -0.534920840050372 1105 3 -0.5750890818333403 1105 4 -0.4072531891529607 1105 5 -0.4922988395560779 1105 6 -0.43185932699211094 1105 7 -0.41933213731176266 1105 8 -0.4672180682091138 1105 9 -0.6231678195490957 1105 10-11 -0.5910363621545969 1105 12-13 -0.6720955373206365 1105 14-15 -0.8788840335117314 1105 16-17 -0.8106953801602117 1105 18-19 -0.8050470246120298 1105 20-21 -0.8700930010895576 1105 22-23 -0.7716637298267344 1105 24-25 -0.8499929522400365 1105 26-27 -0.8282826345354479 1105 28-29 -0.8118144776288929 1105 30-31 -0.8675134306939967 1105 32-33 -0.9119010525713662 1105 34-35 -0.9305433821055331 1105 36-37 -0.9089993718650007 1105 38-39 -0.9529798333418569 1105 40-41 -0.9322952461914866 1105 42-43 -0.9882011656885012 1105 44-45 -0.9937818537776195 1105 46-47 -1.058340187114581 1105 48-49 -1.0664019609157336 1105 50-51 -1.0024581323177557 1105 52-53 -0.8634905986497401 1105 54-55 -0.7841524675020324 1105 56-57 -0.8413973131978381 1105 58-59 -0.8515299587321365 1105 60-61 -0.8084630186042645 1105 62-63 -0.9035171588453252 1105 64-65 -0.8974337425696817 1105 66-67 -0.8693006041276803 1105 68-69 -0.9354007966347453 1105 70-71 -0.9437150903871157 1105 72-73 -0.8665624828233227 1105 74-75 -0.9021751182797786 1105 76-77 -0.9990864386748832 1105 78-79 -1.0942235564301832 1105 80-81 -1.0545459225413047 1105 82-83 -1.1018208965700929 1105 84-85 -1.090795840613044 1105 86-87 -1.131286747655757 1105 88-89 -1.1415092360085737 1105 90-91 -1.2672496635259591 1105 92-93 -1.193225643246116 1105 94-95 -1.327211470307855 1105 96-97 -1.3441353115453545 1105 98-99 -1.417878421632384 1105 100 -1.4344883757463265 1106 1 -0.47109028396439 1106 2 -0.5073553297730271 1106 3 -0.5023874447297452 1106 4 -0.3017929930845824 1106 5 -0.3768177801124537 1106 6 -0.42030061199645274 1106 7 -0.4612674334861637 1106 8 -0.41477517234935846 1106 9 -0.6068455483568371 1106 10-11 -0.7071706923646346 1106 12-13 -0.6143453713852409 1106 14-15 -0.8459792007161226 1106 16-17 -0.7774573453268019 1106 18-19 -0.8038353910786427 1106 20-21 -0.8278040323237548 1106 22-23 -0.7868357535454606 1106 24-25 -0.8664869548900853 1106 26-27 -0.8397642956638336 1106 28-29 -0.8429154388908628 1106 30-31 -0.8513302394536879 1106 32-33 -0.9946128415949431 1106 34-35 -0.8699140722296406 1106 36-37 -0.8362381663720129 1106 38-39 -0.8657597248968756 1106 40-41 -0.9342019261908803 1106 42-43 -0.8428296674332216 1106 44-45 -0.8088039509159017 1106 46-47 -0.8016222716301371 1106 48-49 -0.7878211715974004 1106 50-51 -0.795640360674021 1106 52-53 -0.7347020101713184 1106 54-55 -0.701123379777222 1106 56-57 -0.7516785325227247 1106 58-59 -0.594698067629011 1106 60-61 -0.5722226867678799 1106 62-63 -0.6920499755902298 1106 64-65 -0.6989667125928491 1106 66-67 -0.6160001292927575 1106 68-69 -0.6636632038178405 1106 70-71 -0.6866686680869094 1106 72-73 -0.6997254944601821 1106 74-75 -0.728914979139816 1106 76-77 -0.7422045232323775 1106 78-79 -0.7059762870115662 1106 80-81 -0.7255381823986689 1106 82-83 -0.7352532746819271 1106 84-85 -0.7559799981781801 1106 86-87 -0.7985419179912441 1106 88-89 -0.9016568038159747 1106 90-91 -0.9015503402605454 1106 92-93 -0.8579829401024419 1106 94-95 -0.9105703551384501 1106 96-97 -0.9514243349948792 1106 98-99 -1.0630604315814622 1106 100 -1.079561683881181 1107 1 -0.6067246336827807 1107 2 -0.6224164965039449 1107 3 -0.6720173387181028 1107 4 -0.5094728221119809 1107 5 -0.5230290268485049 1107 6 -0.560367669184771 1107 7 -0.5515077890374869 1107 8 -0.5986193899389889 1107 9 -0.6866909750112384 1107 10-11 -0.7117494513752405 1107 12-13 -0.745283325787014 1107 14-15 -0.8608144767020249 1107 16-17 -0.9698807859733947 1107 18-19 -0.9431694596590603 1107 20-21 -0.9562939900617948 1107 22-23 -0.960026505084862 1107 24-25 -0.9517423034530665 1107 26-27 -1.028126500995775 1107 28-29 -1.0182785385767588 1107 30-31 -0.9839232602848895 1107 32-33 -1.0542850072202299 1107 34-35 -1.061478660680251 1107 36-37 -0.9765199625356402 1107 38-39 -0.9980041232931214 1107 40-41 -1.099744693124002 1107 42-43 -0.9891149251393472 1107 44-45 -1.0725329340127772 1107 46-47 -1.0557568265466202 1107 48-49 -1.1535286984957693 1107 50-51 -1.1504228175091384 1107 52-53 -0.9714042334456536 1107 54-55 -0.9238868588306985 1107 56-57 -0.845770085717426 1107 58-59 -0.7542185332351039 1107 60-61 -0.8382514717462826 1107 62-63 -0.8088559829165192 1107 64-65 -0.8169536805780737 1107 66-67 -0.9005464866394277 1107 68-69 -0.8481974414792646 1107 70-71 -0.8294780028302569 1107 72-73 -0.810200608245566 1107 74-75 -0.8143783604061703 1107 76-77 -0.9344267757211675 1107 78-79 -0.8691809848310967 1107 80-81 -0.9078244362281573 1107 82-83 -0.8930679965666073 1107 84-85 -0.9662557075358187 1107 86-87 -1.043350883267415 1107 88-89 -1.146490347233069 1107 90-91 -1.2082020563194824 1107 92-93 -1.133443992840565 1107 94-95 -1.1098793394963842 1107 96-97 -1.0378441205158602 1107 98-99 -1.1399738000989856 1107 100 -1.037026912206926 1108 1 -0.4491827958970056 1108 2 -0.45985840518988397 1108 3 -0.5393005915641425 1108 4 -0.384749533414535 1108 5 -0.4123481177211161 1108 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1.3222293829425098 2202 30-31 1.3340101747537005 2202 32-33 1.3761846742165602 2202 34-35 1.3819212911853782 2202 36-37 1.3510096336148791 2202 38-39 1.2832788317388406 2202 40-41 1.2957702817484247 2202 42-43 0.6648532512379006 2202 44-45 1.0715638078622973 2202 46-47 1.2024604523417466 2202 48-49 1.1761805797084364 2202 50-51 1.2941402161422957 2202 52-53 1.2014080488674566 2202 54-55 1.160686360349871 2202 56-57 1.1055452296169719 2202 58-59 1.1274141902726598 2202 60-61 1.1103687458274223 2202 62-63 1.08103762304939 2202 64-65 1.0748854388965654 2202 66-67 1.016285106875614 2202 68-69 0.932056667880012 2202 70-71 0.925373510715005 2202 72-73 0.8144221670953371 2202 74-75 0.7815258793368827 2202 76-77 0.7333673562543801 2202 78-79 0.7109694423548092 2202 80-81 0.6979064099068104 2202 82-83 0.6541406700292818 2202 84-85 0.6277903251251225 2202 86-87 0.5537180268544937 2202 88-89 0.5439893403542193 2202 90-91 0.47805440627563556 2202 92-93 0.46827770776418376 2202 94-95 0.4702704919671312 2202 96-97 0.4326093063044105 2202 98-99 0.4036777749777869 2202 100 0.4216388231911914 2203 1 -0.08058289095423632 2203 2 0.13427077064762827 2203 3 0.5217359401933344 2203 4 0.6056725737145499 2203 5 0.6261132198931918 2203 6 0.7363930821538887 2203 7 0.7685917469113477 2203 8 0.7874667883314004 2203 9 0.9910544837171997 2203 10-11 0.974784745052915 2203 12-13 1.0108823243201357 2203 14-15 1.2117813788869043 2203 16-17 1.220976702977211 2203 18-19 1.1857053741927999 2203 20-21 1.1544241355944038 2203 22-23 1.1819075473205416 2203 24-25 1.2308576979432786 2203 26-27 1.175429127295189 2203 28-29 1.213989814849505 2203 30-31 1.1610612935072098 2203 32-33 1.1797577318319554 2203 34-35 1.1670242473692625 2203 36-37 1.117087880653763 2203 38-39 1.176628696806759 2203 40-41 1.1416490245980384 2203 42-43 1.1368986782288957 2203 44-45 1.1515395288495043 2203 46-47 1.1907401905997972 2203 48-49 1.1324351668196755 2203 50-51 1.2576348933269443 2203 52-53 1.2145947731563922 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1.2328474541722798 2204 14-15 1.502221714983726 2204 16-17 1.4469328390783218 2204 18-19 1.429352649115721 2204 20-21 1.4029838152994643 2204 22-23 1.4844916156362515 2204 24-25 1.444206915356844 2204 26-27 1.4580647756467684 2204 28-29 1.4563120417272515 2204 30-31 1.4631092599431668 2204 32-33 1.5161949339124448 2204 34-35 1.5229644016027137 2204 36-37 1.5057569533164141 2204 38-39 1.43729951292012 2204 40-41 1.4681274306175496 2204 42-43 1.4684427389427057 2204 44-45 1.4881828321146173 2204 46-47 1.549446021416344 2204 48-49 1.4608679007231373 2204 50-51 1.601999276083113 2204 52-53 1.4712317337019272 2204 54-55 1.3673543218405797 2204 56-57 1.380806152603327 2204 58-59 1.4006149068473093 2204 60-61 1.3846080127194398 2204 62-63 1.3134871776048058 2204 64-65 1.3640597491586988 2204 66-67 1.31834318793058 2204 68-69 1.2354986221089277 2204 70-71 1.2461038700556202 2204 72-73 1.2176311249470828 2204 74-75 1.1651316060933397 2204 76-77 1.164830089263102 2204 78-79 1.1174502997963742 2204 80-81 1.115014657704581 2204 82-83 1.0723874473804038 2204 84-85 1.0474633487599085 2204 86-87 1.0676618060968401 2204 88-89 1.057089540604725 2204 90-91 1.0496496722420616 2204 92-93 1.0491774899916528 2204 94-95 1.0156421534464606 2204 96-97 0.9867150200957049 2204 98-99 0.9591713368468575 2204 100 0.954106691225455 2205 1 0.961788196030767 2205 2 1.0156417402646305 2205 3 1.0827022951177874 2205 4 0.9316741661880741 2205 5 0.9163422434262429 2205 6 0.9479599282418363 2205 7 1.0656534536592588 2205 8 1.051749018969403 2205 9 1.3052913752222466 2205 10-11 1.3227684380015248 2205 12-13 1.3356497637360434 2205 14-15 1.5662202978928192 2205 16-17 1.5606446155824685 2205 18-19 1.5561230901057215 2205 20-21 1.5582275061861566 2205 22-23 1.625739803249047 2205 24-25 1.6558724942471486 2205 26-27 1.6262450506938464 2205 28-29 1.6638580299384031 2205 30-31 1.6511551419621497 2205 32-33 1.669566896755157 2205 34-35 1.6354275497121549 2205 36-37 1.6781225610180996 2205 38-39 1.7111065004017831 2205 40-41 1.7277261864699511 2205 42-43 1.7327255636316465 2205 44-45 1.7533940198183586 2205 46-47 1.7422568622159744 2205 48-49 1.7298263393259887 2205 50-51 1.7296354761293742 2205 52-53 1.6755098161279705 2205 54-55 1.5865004638958666 2205 56-57 1.561426840253965 2205 58-59 1.5804436049022286 2205 60-61 1.585911974464743 2205 62-63 1.5833288376503631 2205 64-65 1.5584729906642778 2205 66-67 1.5350222753060265 2205 68-69 1.4654180766169205 2205 70-71 1.5200424978677631 2205 72-73 1.2927923741872362 2205 74-75 1.3923551391934552 2205 76-77 1.3795532121274512 2205 78-79 1.3570070212346792 2205 80-81 1.3440546438797973 2205 82-83 1.3006172935033185 2205 84-85 1.3103972640421269 2205 86-87 1.2885271227031474 2205 88-89 1.35279144159243 2205 90-91 1.3744696178361444 2205 92-93 1.2867504113536192 2205 94-95 1.224351480629366 2205 96-97 1.2889641379376435 2205 98-99 1.2761821866289296 2205 100 1.3507842631785145 2206 1 0.9625270741983982 2206 2 0.9905170022272785 2206 3 1.0269331928087944 2206 4 0.9316170088446114 2206 5 0.9050357527992929 2206 6 0.9582365040062655 2206 7 1.0256839443850794 2206 8 0.9999705328872537 2206 9 1.2159085458356884 2206 10-11 1.255158430032587 2206 12-13 1.2879799264506246 2206 14-15 1.5169142754739866 2206 16-17 1.503270256023555 2206 18-19 1.4863817546625882 2206 20-21 1.4708897677743664 2206 22-23 1.565208685283686 2206 24-25 1.5341142379424255 2206 26-27 1.5632964712621984 2206 28-29 1.5355098771644649 2206 30-31 1.586569468354277 2206 32-33 1.5859208629089352 2206 34-35 1.61381890755451 2206 36-37 1.5783393728354085 2206 38-39 1.580287375203305 2206 40-41 1.5351474689802345 2206 42-43 1.5688365028076916 2206 44-45 1.6566241757712987 2206 46-47 1.6935624315433557 2206 48-49 1.6645009397914592 2206 50-51 1.6429546896786462 2206 52-53 1.595624237040127 2206 54-55 1.472517761448465 2206 56-57 1.5284288109362123 2206 58-59 1.5648535207003533 2206 60-61 1.4568438393568712 2206 62-63 1.5050871348109425 2206 64-65 1.4758870233027963 2206 66-67 1.4136361954275145 2206 68-69 1.3813400533703444 2206 70-71 1.3880589092568272 2206 72-73 1.3389509226930443 2206 74-75 1.335785057814718 2206 76-77 1.343916885224214 2206 78-79 1.2984405286343872 2206 80-81 1.2937225470489615 2206 82-83 1.3458092026545962 2206 84-85 1.3422115766249725 2206 86-87 1.3372107061458216 2206 88-89 1.311655742745856 2206 90-91 1.3540568061749063 2206 92-93 1.312475730483822 2206 94-95 1.2738518747862422 2206 96-97 1.2980588150527872 2206 98-99 1.2577398529342219 2206 100 1.2883297127655524 2207 1 0.9239819966002827 2207 2 0.94371734082263 2207 3 0.9861321851897316 2207 4 0.8981875460283462 2207 5 0.8611235573083391 2207 6 0.8583404992372792 2207 7 0.9248040851518482 2207 8 0.9296161357499884 2207 9 1.177200362746511 2207 10-11 1.215481755791373 2207 12-13 1.2171036620316116 2207 14-15 1.490991129522854 2207 16-17 1.475169703647211 2207 18-19 1.4128650590265366 2207 20-21 1.414193489076517 2207 22-23 1.4745437216351576 2207 24-25 1.4987310237166085 2207 26-27 1.5520561666334842 2207 28-29 1.5478276888664908 2207 30-31 1.5159770235559336 2207 32-33 1.5579262470971713 2207 34-35 1.5852332226135317 2207 36-37 1.601472019137482 2207 38-39 1.5786841349167489 2207 40-41 1.6164097173725267 2207 42-43 1.688732996257194 2207 44-45 1.6667538702422036 2207 46-47 1.6772342430786935 2207 48-49 1.7169222346140955 2207 50-51 1.7110924353284744 2207 52-53 1.6731500877375112 2207 54-55 1.554000501821541 2207 56-57 1.5469186408586566 2207 58-59 1.5313061218697754 2207 60-61 1.5326532332022467 2207 62-63 1.5066572050704892 2207 64-65 1.4716918851384193 2207 66-67 1.4783901297240831 2207 68-69 1.433839520340392 2207 70-71 1.4394166545250755 2207 72-73 1.3982889379874024 2207 74-75 1.3718583491817782 2207 76-77 1.302312634105359 2207 78-79 1.3072494635886756 2207 80-81 1.3168933338614188 2207 82-83 1.3135003746410199 2207 84-85 1.2768999105825465 2207 86-87 1.3350986691078575 2207 88-89 1.372290752956264 2207 90-91 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52-53 1.488397003635086 2310 54-55 1.4838322708985814 2310 56-57 1.4689968370313977 2310 58-59 1.4826665236548493 2310 60-61 1.4951641551310786 2310 62-63 1.4739012423391458 2310 64-65 1.4754490609085522 2310 66-67 1.5155808890079356 2310 68-69 1.471290012864074 2310 70-71 1.4033576476636611 2310 72-73 1.3326091247564165 2310 74-75 1.3590428710493043 2310 76-77 1.3961319608591474 2310 78-79 1.4090374283293983 2310 80-81 1.4688404784986524 2310 82-83 1.500685335127283 2310 84-85 1.5418706643888207 2310 86-87 1.578926207062242 2310 88-89 1.579831015252843 2310 90-91 1.6074098852109096 2310 92-93 1.6445686120392473 2310 94-95 1.6899354847537502 2310 96-97 1.7963968310955067 2310 98-99 1.7840262828134215 2310 100 1.9274419864894021 2311 1 0.6376617793859687 2311 2 0.7101301246492611 2311 3 0.7693545157335784 2311 4 0.6646512612295936 2311 5 0.6403053192320272 2311 6 0.63420174968509 2311 7 0.6728118466392417 2311 8 0.6887952582209778 2311 9 0.8856662718154311 2311 10-11 0.7886007877546817 2311 12-13 0.9173145537860634 2311 14-15 1.2270588829577278 2311 16-17 1.1661998732623644 2311 18-19 1.116407776039928 2311 20-21 1.1786973783990788 2311 22-23 1.2309890625818838 2311 24-25 1.2244695447742373 2311 26-27 1.2632728859537536 2311 28-29 1.3454539359143567 2311 30-31 1.3310710292246952 2311 32-33 1.3252195322185685 2311 34-35 1.3659108743572332 2311 36-37 1.3887205793392567 2311 38-39 1.4580179169312402 2311 40-41 1.4527026628224178 2311 42-43 1.5345779933010348 2311 44-45 1.4956194848173539 2311 46-47 1.5396081676081437 2311 48-49 1.5889534082791172 2311 50-51 1.592760049540594 2311 52-53 1.410383663949517 2311 54-55 1.3321727504920418 2311 56-57 1.2947868957895352 2311 58-59 1.2805464664847648 2311 60-61 1.2749005375354017 2311 62-63 1.2744808834380805 2311 64-65 1.2985791228428027 2311 66-67 1.3137387419537845 2311 68-69 1.3400370352556905 2311 70-71 1.2700401949083755 2311 72-73 1.3455914973147785 2311 74-75 1.3619807779891175 2311 76-77 1.2776628485834571 2311 78-79 1.2940620432253809 2311 80-81 1.3501728589973006 2311 82-83 1.3873774107188375 2311 84-85 1.417327231007846 2311 86-87 1.4771714031873806 2311 88-89 1.5397325556689125 2311 90-91 1.5829934964543604 2311 92-93 1.582832864858048 2311 94-95 1.673340280688322 2311 96-97 1.7141751382258867 2311 98-99 1.7824779264532538 2311 100 1.7798320134863843 2312 1 0.6224868354994157 2312 2 0.6771668264672712 2312 3 0.7500096503390665 2312 4 0.6297874668143422 2312 5 0.6437786736649755 2312 6 0.4836154756889499 2312 7 0.6375945485248948 2312 8 0.7144953834709966 2312 9 0.888784166164406 2312 10-11 0.9166161364970264 2312 12-13 0.9952447188970481 2312 14-15 1.1822207800144824 2312 16-17 1.2119086521294307 2312 18-19 1.153312634823365 2312 20-21 1.1838423884682499 2312 22-23 1.2083215784473182 2312 24-25 1.2609724323042641 2312 26-27 1.2754216128155988 2312 28-29 1.277766094654524 2312 30-31 1.2078398504091226 2312 32-33 1.322274900205727 2312 34-35 1.4003130559972519 2312 36-37 1.363562598438314 2312 38-39 1.3807873475295622 2312 40-41 1.4005504362890022 2312 42-43 1.490721788643313 2312 44-45 1.502692371102441 2312 46-47 1.440439159017096 2312 48-49 1.4718066522869222 2312 50-51 1.4532149722319758 2312 52-53 1.3143398297165731 2312 54-55 1.258808637204865 2312 56-57 1.2169722514435648 2312 58-59 1.2668009909540103 2312 60-61 1.2447518282057715 2312 62-63 1.2440433533845265 2312 64-65 1.25328684703031 2312 66-67 1.2047418245015251 2312 68-69 1.2564726603923262 2312 70-71 1.30804262323349 2312 72-73 1.3257054093294514 2312 74-75 1.3217458887767464 2312 76-77 1.311828655663522 2312 78-79 1.3020852340612805 2312 80-81 1.1529261659743675 2312 82-83 1.2831542040587856 2312 84-85 1.4073832558512258 2312 86-87 1.4613676696751448 2312 88-89 1.482927349864184 2312 90-91 1.5543194968809253 2312 92-93 1.5190417096214297 2312 94-95 1.6663623637212837 2312 96-97 1.6647586902134464 2312 98-99 1.601297780915523 2312 100 1.7061454352987262 2313 1 0.5739208787248486 2313 2 0.635082370430748 2313 3 0.6962845315086774 2313 4 0.6380693273675888 2313 5 0.6232518428422011 2313 6 0.6881258641252685 2313 7 0.7405457206055743 2313 8 0.7641286120596575 2313 9 0.9125786515189489 2313 10-11 0.8214932229863763 2313 12-13 0.9416978057581673 2313 14-15 1.154807932354288 2313 16-17 1.1215941287121964 2313 18-19 1.1337975068895787 2313 20-21 1.1682260991952802 2313 22-23 1.2001266933486292 2313 24-25 1.2028970002210286 2313 26-27 1.2539974813991606 2313 28-29 1.2603244487198353 2313 30-31 1.2423476669028517 2313 32-33 1.3463558956370036 2313 34-35 1.308986802181657 2313 36-37 1.3302509168122398 2313 38-39 1.3655893015310312 2313 40-41 1.4220996161265091 2313 42-43 1.3876228820820344 2313 44-45 1.3426728299139654 2313 46-47 1.4082009204735257 2313 48-49 1.4995672733883154 2313 50-51 1.4472387819208379 2313 52-53 1.3135136683507938 2313 54-55 1.2713659396787023 2313 56-57 1.279450631372086 2313 58-59 1.2843064127755142 2313 60-61 1.2576260386089402 2313 62-63 1.2679834183191119 2313 64-65 1.2646817044435679 2313 66-67 1.2757226259330636 2313 68-69 1.3513593784619005 2313 70-71 1.3720962171326292 2313 72-73 1.3744363397891064 2313 74-75 1.3641351852994603 2313 76-77 1.4097293093558143 2313 78-79 1.3699657296216543 2313 80-81 1.381386917019018 2313 82-83 1.399695664583998 2313 84-85 1.4742534606702158 2313 86-87 1.5374319457189713 2313 88-89 1.5718379876600999 2313 90-91 1.6696498757814027 2313 92-93 1.7423203374570448 2313 94-95 1.8081453482523244 2313 96-97 1.8704042894351751 2313 98-99 1.8833442883278835 2313 100 2.01404494496807 2314 1 0.5365643265462268 2314 2 0.5623918982996656 2314 3 0.5989962679492287 2314 4 0.5559641169024374 2314 5 0.5354847544692376 2314 6 0.5594208779460033 2314 7 0.6428723561867145 2314 8 0.5715906848076955 2314 9 0.7811850285515689 2314 10-11 0.7143822071216874 2314 12-13 0.5087032121900492 2314 14-15 0.9104705497842147 2314 16-17 0.9395459453630579 2314 18-19 0.9304095707446365 2314 20-21 0.9823632766478809 2314 22-23 0.8314236502109011 2314 24-25 0.8333110612494394 2314 26-27 1.0121420557874004 2314 28-29 1.0921155283010293 2314 30-31 1.0328788094348127 2314 32-33 1.0979208451719558 2314 34-35 1.1373907618088523 2314 36-37 1.210685874622719 2314 38-39 1.2774956100072359 2314 40-41 1.2494125663226683 2314 42-43 1.3525438920043484 2314 44-45 1.368360773531407 2314 46-47 1.310555334920096 2314 48-49 1.409977761045866 2314 50-51 1.3371453276961063 2314 52-53 1.1963502257409644 2314 54-55 1.1479552647681217 2314 56-57 1.0887621613960121 2314 58-59 1.116749415813885 2314 60-61 1.1255877183719107 2314 62-63 1.1077254140848893 2314 64-65 1.1029922027224544 2314 66-67 1.1701169390285173 2314 68-69 1.1869298688225385 2314 70-71 1.234928317951244 2314 72-73 1.2738044854529207 2314 74-75 1.3111502186793231 2314 76-77 1.297629721794241 2314 78-79 1.3476018956555151 2314 80-81 1.383938289447105 2314 82-83 1.2962930382095372 2314 84-85 1.3300957191903144 2314 86-87 1.5604981325136578 2314 88-89 1.699078511776868 2314 90-91 1.723143455528092 2314 92-93 1.7351431787343365 2314 94-95 1.8263073201850872 2314 96-97 1.8688404416372535 2314 98-99 1.933805785142141 2314 100 1.8324476950389226 2315 1 0.36948067506997617 2315 2 0.40989297403355707 2315 3 0.4435196480984658 2315 4 0.3743029153622075 2315 5 0.28295088067385166 2315 6 -0.38850887873186224 2315 7 0.1790112205924288 2315 8 0.2621737315001411 2315 9 0.45625352749795667 2315 10-11 0.5775940917286917 2315 12-13 0.5717835030139966 2315 14-15 0.6889390412505705 2315 16-17 0.6447240061094206 2315 18-19 0.7281649305523956 2315 20-21 0.6954307330105323 2315 22-23 0.6383239393104176 2315 24-25 0.725863367799839 2315 26-27 0.8042874103684667 2315 28-29 0.8856344922845878 2315 30-31 0.8944286968499924 2315 32-33 0.9314217390289485 2315 34-35 0.9853125805271361 2315 36-37 0.9770784615010868 2315 38-39 1.048390955220306 2315 40-41 1.0564622555911498 2315 42-43 1.1312762019552522 2315 44-45 1.0760296362172355 2315 46-47 1.150670199427914 2315 48-49 1.1666112877738541 2315 50-51 1.1657803199745942 2315 52-53 0.94288486133145 2315 54-55 0.7887309343312054 2315 56-57 0.8253835269616232 2315 58-59 0.761250137970066 2315 60-61 0.672064585418191 2315 62-63 0.8853954884453898 2315 64-65 0.9233180770036213 2315 66-67 0.9290684475233775 2315 68-69 1.001031143107781 2315 70-71 1.0371027107030244 2315 72-73 1.049441389234694 2315 74-75 1.0616849040968077 2315 76-77 1.0843934588340396 2315 78-79 1.0548669615627944 2315 80-81 1.0641440657101136 2315 82-83 1.2340867789287806 2315 84-85 1.1842011192272004 2315 86-87 1.302875970362642 2315 88-89 1.4622892614490013 2315 90-91 1.4728556842909306 2315 92-93 1.5878179682224705 2315 94-95 1.6186361080164602 2315 96-97 1.6822394091242678 2315 98-99 1.816492362030349 2315 100 1.906360580071862 2316 1 0.036480892543366394 2316 2 -0.06632449016228747 2316 3 0.07655317110362603 2316 4 0.005095892661032053 2316 5 0.05055327719789915 2316 6 0.12528901881056953 2316 7 0.08516789956647841 2316 8 0.13215771395366716 2316 9 0.19233568078919205 2316 10-11 0.11122957107508569 2316 12-13 0.22876542111696097 2316 14-15 0.41964006976298407 2316 16-17 0.4129706222351004 2316 18-19 0.37592980568833667 2316 20-21 0.3479118919704973 2316 22-23 0.2511910168696616 2316 24-25 0.2929873826926652 2316 26-27 0.3880122868198228 2316 28-29 0.48536468691865053 2316 30-31 0.4977042656753454 2316 32-33 0.5307824788582991 2316 34-35 0.499094808447353 2316 36-37 0.6473595176024602 2316 38-39 0.7017366176923616 2316 40-41 0.6611819914113326 2316 42-43 0.7295880544113018 2316 44-45 0.5322750518680195 2316 46-47 0.560805274355296 2316 48-49 0.5891583797419244 2316 50-51 0.3906894728891004 2316 52-53 0.16203338016093483 2316 54-55 0.13312573958165075 2316 56-57 0.24910279611077613 2316 58-59 0.2655890151965963 2316 60-61 0.32421192900527984 2316 62-63 0.4274576499403864 2316 64-65 0.4606390953268473 2316 66-67 0.42784811535131695 2316 68-69 0.45822570073850244 2316 70-71 0.388843011938377 2316 72-73 0.46173270605702754 2316 74-75 0.5635966246614856 2316 76-77 0.6260265167129369 2316 78-79 0.6844215470871546 2316 80-81 0.7856116856663284 2316 82-83 0.8245413982931318 2316 84-85 0.9174625480176495 2316 86-87 0.9853434672837409 2316 88-89 1.0523796028182524 2316 90-91 1.1468273910244058 2316 92-93 1.2075132665397383 2316 94-95 1.286865660809056 2316 96-97 1.323535567460059 2316 98-99 1.495490060849633 2316 100 1.3948105847546834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 65.0 1 625.0 2 299.0 3 219.0 4 195.0 5 165.0 6 201.0 7 1094.0 8 3772.0 9 7051.0 10 10870.0 11 17300.0 12 24315.0 13 30481.0 14 35944.0 15 42253.0 16 50413.0 17 60866.0 18 72988.0 19 85351.0 20 99425.0 21 114713.0 22 124914.0 23 129978.0 24 133692.0 25 143638.0 26 159934.0 27 181707.0 28 212164.0 29 248730.0 30 293087.0 31 354304.0 32 436002.0 33 556932.0 34 755048.0 35 966924.0 36 1357051.0 37 1887500.0 38 1668703.0 39 413213.0 40 441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.226131124708104 18.761693691722694 12.304322548547672 23.707852635021535 2 16.176580055646504 20.276623762095912 40.98820263367451 22.558593548583065 3 18.210131231949227 27.76355121678997 30.22627925518343 23.800038296077368 4 12.316807883961529 16.725600187938085 38.25188928244486 32.70570264565553 5 12.470698792482187 38.33817090799519 33.975093060653805 15.216037238868818 6 29.272583593583967 36.66221548555823 20.10625700189958 13.95894391895822 7 27.21912452253185 33.2457774060559 22.97023288933717 16.564865182075085 8 26.80528966992529 35.06642527857461 20.660664559063513 17.467620492436577 9 29.110701627447828 15.217964203977058 20.19809802372104 35.47323614485408 10-11 25.263170316115307 25.7228017381294 29.191493362887798 19.822534582867487 12-13 25.374644769133297 23.99790127567039 28.335779972026376 22.291673983169936 14-15 23.256708227395062 25.43171083732704 26.428068168146098 24.883512767131805 16-17 22.35992888918018 27.478789056236767 27.308876797812836 22.85240525677022 18-19 21.904144046230726 27.40702918318877 28.875647434807806 21.8131793357727 20-21 23.169131918661595 27.049711073692766 28.046855728697707 21.73430127894793 22-23 22.89325225915509 26.56324337412979 28.191457734986162 22.35204663172895 24-25 22.152352699689345 26.184769673783936 28.66047096455755 23.002406661969165 26-27 22.106522422624284 26.51833476929461 28.430961222678736 22.94418158540237 28-29 22.0254694259403 27.870527874558586 28.101238220111956 22.002764479389153 30-31 23.618003364957442 26.654301649615615 28.32930424776723 21.398390737659717 32-33 23.3729576147564 26.53724143426459 28.036085365820547 22.053715585158464 34-35 23.086882653502887 26.9364396100849 28.34300876816921 21.633668968243004 36-37 22.590437213563348 26.912483082005124 28.30498229535845 22.192097409073078 38-39 22.666693757773576 27.274038983946784 28.085882679058916 21.973384579220724 40-41 22.744577140459043 26.322008988488825 28.637465282856123 22.295948588196012 42-43 22.40658219863833 26.841425241599858 27.745165661787535 23.00682689797427 44-45 21.595296477372916 27.195261527693383 28.575816011101864 22.63362598383184 46-47 21.486515955751827 26.31247536615891 28.382202675245093 23.818806002844166 48-49 21.9588823283747 26.52114590134143 28.44563918360268 23.07433258668119 50-51 21.952957286521094 26.855833698363384 28.3913991740801 22.79980984103542 52-53 21.59149260964948 27.236891580877103 27.859394864566823 23.312220944906592 54-55 21.411967189349014 26.625955844149036 28.604688557665693 23.357388408836265 56-57 22.047884105579296 26.537494877768133 27.94017280002278 23.4744482166298 58-59 21.465778699646265 26.722949990814666 28.565340917738148 23.245930391800922 60-61 22.311393177244497 26.854750116398318 28.02878953475552 22.805067171601667 62-63 22.240253728465053 26.620210269637525 27.99793740750907 23.14159859438835 64-65 21.726593652474186 26.16299917287037 28.12699077956698 23.983416395088458 66-67 21.79874036213348 26.019329716986476 27.83434204808064 24.347587872799405 68-69 21.12110124853879 26.262082176546215 28.375735568648565 24.241081006266434 70-71 21.483979225394023 25.863441400254665 28.627897940754032 24.024681433597276 72-73 21.461912700286863 26.389159505415847 28.0517618903595 24.09716590393779 74-75 21.71784631069103 26.325910370125673 27.904661796178065 24.05158152300523 76-77 21.370635664012745 25.879627150217388 28.139108592555363 24.610628593214507 78-79 21.029353770629452 25.948855514163803 28.52188415561517 24.499906559591576 80-81 21.26018091667294 25.995470529038762 28.241184965631977 24.503163588656324 82-83 21.908735281926646 25.72826942132155 28.43235390582699 23.930641390924816 84-85 22.008631908101073 26.10289009764729 27.7555915478453 24.13288644640634 86-87 21.285010316386025 26.356564035836872 28.186723122954206 24.171702524822898 88-89 21.36528676165021 25.414200661271686 28.37831328110067 24.842199295977434 90-91 22.263799426585066 25.00365037877566 28.430985042365226 24.301565152274048 92-93 22.285459693298264 25.201293231563515 28.235465294692563 24.27778178044566 94-95 21.79041189722764 25.093985838020433 28.28632313113186 24.829279133620076 96-97 21.792099626634208 24.897636910544367 28.385126230410002 24.92513723241143 98-99 21.657606891565585 25.079146423763376 28.06780939909958 25.195437285571458 100 21.84376579589591 25.03262885471362 27.96150984371673 25.162095505673744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1214.0 1 901.0 2 1609.0 3 2459.5 4 1852.0 5 1496.0 6 1971.5 7 3399.5 8 5527.5 9 6771.0 10 6480.5 11 6092.0 12 6620.0 13 7488.5 14 8372.0 15 9360.0 16 10380.0 17 11463.5 18 12428.0 19 13450.5 20 14696.5 21 16321.0 22 18759.0 23 22026.5 24 26355.5 25 31856.0 26 39813.5 27 50044.0 28 61550.5 29 77738.0 30 99686.0 31 120830.5 32 147674.0 33 176548.0 34 203420.5 35 231641.0 36 260938.0 37 296488.0 38 313121.5 39 310455.5 40 309196.0 41 309508.5 42 313083.0 43 324608.0 44 343747.0 45 371498.0 46 402560.5 47 428249.5 48 454649.5 49 481449.5 50 499288.0 51 500851.0 52 478582.5 53 443285.0 54 407215.5 55 361007.5 56 318287.5 57 278649.0 58 233315.5 59 190149.0 60 147197.0 61 111817.5 62 86886.0 63 66953.0 64 49091.0 65 34353.0 66 24603.0 67 18191.0 68 13818.0 69 10545.5 70 8005.5 71 6006.5 72 4492.0 73 3410.0 74 2565.5 75 1861.0 76 1318.5 77 926.0 78 644.0 79 454.5 80 342.5 81 256.0 82 196.5 83 157.0 84 132.0 85 106.0 86 80.5 87 60.0 88 50.5 89 45.5 90 29.0 91 21.5 92 22.5 93 18.0 94 10.5 95 8.5 96 9.5 97 7.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22005946698017434 2 0.14929932103397994 3 0.024544662345670286 4 0.02297200663473489 5 0.027830389456017457 6 0.05616627539054986 7 0.06294367262100954 8 0.05601649865617505 9 0.04325739309662181 10-11 0.04569126503021231 12-13 0.043547585519472985 14-15 0.020706633527316047 16-17 0.019232268798314113 18-19 0.01920418566061884 20-21 0.02266777264303608 22-23 0.026210928515589933 24-25 0.02266777264303608 26-27 0.020116887635715272 28-29 0.019424170239231823 30-31 0.01775790406931218 32-33 0.026547926167933233 34-35 0.02674918865474937 36-37 0.025653946284633647 38-39 0.023922152793425026 40-41 0.02513440823727106 42-43 0.031256532254841 44-45 0.04079075750238683 46-47 0.04613591471038749 48-49 0.041726862092229325 50-51 0.028841382413047352 52-53 0.04485813194525248 54-55 0.04278934080170056 56-57 0.04538703103851349 58-59 0.048850618020930735 60-61 0.02475528587838485 62-63 0.02624369217623442 64-65 0.026795993884241497 66-67 0.032445385083940965 68-69 0.03673274410541961 70-71 0.04060821710736755 72-73 0.044825368284608 74-75 0.02560246053219231 76-77 0.018899951668920025 78-79 0.018375733098608227 80-81 0.02144615615329162 82-83 0.01943353128513025 84-85 0.011860445153304445 86-87 0.01405092989353589 88-89 0.01952246122116529 90-91 0.02029942803073456 92-93 0.020505371040499907 94-95 0.02465231437350217 96-97 0.025199935558560035 98-99 0.030985061923786666 100 0.03153736363179374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.0682567E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.725413774262655 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98048835533496 44.619856174375904 2 14.234078418832603 16.71804289682494 3 4.6557015502271035 8.202239998396882 4 1.934689778000873 4.544618309517505 5 0.9988893718279441 2.933009583765466 6 0.5662500015155901 1.995195938320793 7 0.34856713362306885 1.432882440508639 8 0.23709964836057992 1.1139019964565784 9 0.1701983733892933 0.8995472910893433 >10 0.7987350874333126 8.117885379904886 >50 0.04055737174889164 1.6543597381868311 >100 0.02973573793973094 3.439275482857533 >500 0.0031727643770974616 1.3011152772248535 >1k 0.0017111978032802603 1.8228662753483547 >5k 2.0868264288239437E-5 0.09033141741376471 >10k+ 1.0434132144119717E-4 1.114871799807681 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30797 0.288292130533794 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23188 0.2170639322926783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15369 0.14386991441289346 No Hit GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 10930 0.10231623166978498 No Hit GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 10689 0.10006021960826457 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.8722091796849952E-5 2 0.0 0.0 0.0 0.0 1.8722091796849952E-5 3 0.0 0.0 0.0 0.0 1.8722091796849952E-5 4 0.0 0.0 0.0 4.680522949212488E-5 1.8722091796849952E-5 5 0.0 0.0 0.0 5.616627539054985E-5 2.8083137695274926E-5 6 0.0 0.0 0.0 5.616627539054985E-5 5.616627539054985E-5 7 0.0 0.0 0.0 5.616627539054985E-5 5.616627539054985E-5 8 0.0 0.0 0.0 5.616627539054985E-5 5.616627539054985E-5 9 0.0 0.0 0.0 9.361045898424976E-5 5.616627539054985E-5 10-11 0.0 0.0 0.0 1.2169359667952469E-4 6.552732128897483E-5 12-13 0.0 0.0 0.0 1.3573516552716215E-4 1.4041568847637464E-4 14-15 0.0 4.680522949212488E-6 0.0 1.4509621142558713E-4 1.7785987207007454E-4 16-17 9.361045898424976E-6 9.361045898424976E-6 0.0 1.91901440917712E-4 2.0594300976534946E-4 18-19 9.361045898424976E-6 9.361045898424976E-6 0.0 2.5274823925747436E-4 2.387066704098369E-4 20-21 9.361045898424976E-6 9.361045898424976E-6 0.0 4.961354326165237E-4 2.714703310543243E-4 22-23 9.361045898424976E-6 9.361045898424976E-6 0.0 0.0014369205454082337 2.9955346874959923E-4 24-25 9.361045898424976E-6 9.361045898424976E-6 0.0 0.0037959041118113277 3.089145146480242E-4 26-27 9.361045898424976E-6 9.361045898424976E-6 0.0 0.007666696590810056 3.182755605464492E-4 28-29 9.361045898424976E-6 1.8722091796849952E-5 0.0 0.020299428030734562 3.182755605464492E-4 30-31 9.361045898424976E-6 1.8722091796849952E-5 0.0 0.07036230149551133 3.182755605464492E-4 32-33 9.361045898424976E-6 1.8722091796849952E-5 0.0 0.1810987939509296 3.2763660644487415E-4 34-35 1.4041568847637465E-5 1.8722091796849952E-5 0.0 0.335518607091348 3.557197441401491E-4 36-37 1.8722091796849952E-5 2.808313769527493E-5 0.0 0.5665539003874256 3.6508079003857405E-4 38-39 1.8722091796849952E-5 3.7444183593699904E-5 0.0 0.9669866802614017 3.83802881835424E-4 40-41 1.8722091796849952E-5 3.7444183593699904E-5 0.0 1.4718653297470543 3.83802881835424E-4 42-43 1.8722091796849952E-5 3.7444183593699904E-5 0.0 1.9262083729500596 4.165665424799114E-4 44-45 1.8722091796849952E-5 3.7444183593699904E-5 0.0 2.4099263781823224 4.3528863427676136E-4 46-47 1.8722091796849952E-5 3.7444183593699904E-5 0.0 2.9473861479174435 4.3996915722597385E-4 48-49 1.8722091796849952E-5 3.7444183593699904E-5 9.361045898424976E-6 3.533879076068514 4.4464968017518633E-4 50-51 2.340261474606244E-5 3.7444183593699904E-5 9.361045898424976E-6 4.167851229016396 4.493302031243988E-4 52-53 2.8083137695274926E-5 3.7444183593699904E-5 9.361045898424976E-6 4.8166512786673845 4.914549096673112E-4 54-55 3.276366064448742E-5 3.7444183593699904E-5 9.361045898424976E-6 5.535027301958415 5.148575244133737E-4 56-57 3.7444183593699904E-5 3.7444183593699904E-5 9.361045898424976E-6 6.252228513989193 5.242185703117987E-4 58-59 3.7444183593699904E-5 3.7444183593699904E-5 9.361045898424976E-6 7.0585047582664355 5.335796162102236E-4 60-61 3.7444183593699904E-5 5.1485752441337365E-5 9.361045898424976E-6 7.861453150726787 5.382601391594362E-4 62-63 3.7444183593699904E-5 5.616627539054985E-5 9.361045898424976E-6 8.700310515253497 5.897458916007735E-4 64-65 3.7444183593699904E-5 5.616627539054985E-5 9.361045898424976E-6 9.498615828948228 5.897458916007735E-4 66-67 3.7444183593699904E-5 5.616627539054985E-5 9.361045898424976E-6 10.346277257142408 5.94426414549986E-4 68-69 3.7444183593699904E-5 5.616627539054985E-5 9.361045898424976E-6 11.28413704309086 5.991069374991985E-4 70-71 3.7444183593699904E-5 5.616627539054985E-5 9.361045898424976E-6 12.247496318066622 5.991069374991985E-4 72-73 4.680522949212488E-5 5.616627539054985E-5 9.361045898424976E-6 13.100306321504934 6.459121669913233E-4 74-75 4.680522949212488E-5 5.616627539054985E-5 9.361045898424976E-6 13.923741362913988 6.693147817373858E-4 76-77 4.680522949212488E-5 5.616627539054985E-5 9.361045898424976E-6 14.805224249939176 6.739953046865983E-4 78-79 4.680522949212488E-5 5.616627539054985E-5 9.361045898424976E-6 15.771831807841693 6.739953046865983E-4 80-81 4.680522949212488E-5 5.616627539054985E-5 9.361045898424976E-6 16.669368888582678 6.786758276358107E-4 82-83 4.680522949212488E-5 6.0846798339762347E-5 9.361045898424976E-6 17.575813004496016 7.395226259755731E-4 84-85 4.680522949212488E-5 6.552732128897483E-5 9.361045898424976E-6 18.462070024929403 7.535641948232105E-4 86-87 4.680522949212488E-5 7.488836718739981E-5 9.361045898424976E-6 19.343707369211913 7.86327855467698E-4 88 4.680522949212488E-5 8.424941308582478E-5 9.361045898424976E-6 20.011632035633383 8.237720390613978E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 11810 0.0 55.0746 2 GGTATCA 17090 0.0 47.057278 1 ACCTGGG 13690 0.0 46.596645 3 GTATCAA 30895 0.0 40.45614 1 CCTGGGG 12625 0.0 36.712814 4 GTACATG 46585 0.0 33.596104 1 TACATGG 47005 0.0 32.8415 2 ATCAACG 37580 0.0 32.72346 3 CAACGCA 37750 0.0 32.712616 5 TATCAAC 38695 0.0 32.08582 2 CTGGGGG 9930 0.0 31.859426 5 TGGGGGG 31605 0.0 31.844694 6 GTACCTG 21010 0.0 31.438175 1 ACATGGG 46960 0.0 31.432564 3 TCAACGC 39680 0.0 31.050423 4 CATGGGG 35590 0.0 30.907227 4 AACGCAG 40410 0.0 30.815508 6 ATGGGGG 28635 0.0 30.550713 5 ACGCAGA 44670 0.0 27.39306 7 CGCAGAG 45075 0.0 26.98999 8 >>END_MODULE