FastQCFastQC Report
Mon 6 Jun 2016
ERR1211405_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211405_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10682567
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281390.26341047053578037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232320.21747581831220905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153080.14329889061308954No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG122350.055.1963272
ACCTGGG136500.048.0980263
GGTATCA175850.047.5964931
GTATCAA306650.039.070941
CCTGGGG123850.038.364254
CTGGGGG90200.035.370845
TGGGGGG286650.033.881026
GTACATG461200.032.3604851
TACATGG468350.031.4876162
GTACCTG219900.031.0483651
ATCAACG380500.030.9150283
CAACGCA381750.030.8818825
ATGGGGG275800.030.8536365
ACATGGG455700.030.7326983
CATGGGG346250.030.5396694
TATCAAC396650.030.0468642
TCAACGC401900.029.3746854
AACGCAG409150.029.1803176
ACGCAGA458700.025.7312477
CGCAGAG465100.025.2256378
Produced by FastQC (version 0.11.5)