FastQCFastQC Report
Mon 6 Jun 2016
ERR1211404_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211404_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7205260
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191690.26604175283057097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167970.23312135856304977No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111170.15429006031704615No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG71800.052.3117752
GGTATCA108150.051.1847041
ACCTGGG82300.044.380583
GTATCAA182100.039.6607631
CCTGGGG81200.035.660754
GTACATG277950.035.3062361
TACATGG285450.033.9011232
TGGGGGG196700.032.8367546
ACATGGG289950.032.6680873
CATGGGG225350.031.6233164
CAACGCA221950.031.4728055
ATCAACG225750.031.0887953
ATGGGGG183600.030.903415
CTGGGGG67500.030.6422545
TATCAAC235100.030.33062
AACGCAG239850.029.5947766
GTACCTG128200.029.5826241
TCAACGC241350.029.1373524
ACGCAGA269550.025.8806637
CGCAGAG273550.025.2779438
Produced by FastQC (version 0.11.5)