##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211404_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7205260 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.835484770847962 31.0 31.0 34.0 27.0 34.0 2 30.864660123298812 31.0 31.0 34.0 27.0 34.0 3 30.914345353255815 31.0 31.0 34.0 27.0 34.0 4 34.538252609898876 37.0 35.0 37.0 30.0 37.0 5 34.59227647579685 37.0 35.0 37.0 30.0 37.0 6 34.661702284164626 37.0 35.0 37.0 30.0 37.0 7 34.56554919600403 37.0 35.0 37.0 30.0 37.0 8 34.565333797808826 37.0 35.0 37.0 30.0 37.0 9 36.055407854817176 39.0 35.0 39.0 30.0 39.0 10-11 35.95425265708663 39.0 35.0 39.0 30.0 39.0 12-13 35.82792140741625 39.0 35.0 39.0 30.0 39.0 14-15 36.91515642738777 40.0 36.0 41.0 30.0 41.0 16-17 36.88651152075012 39.5 36.0 41.0 30.0 41.0 18-19 36.87711068025304 39.5 36.0 41.0 30.0 41.0 20-21 36.8604289782742 39.5 36.0 41.0 30.0 41.0 22-23 36.731685046757505 39.0 36.0 41.0 29.5 41.0 24-25 36.626480238048316 39.0 36.0 41.0 29.0 41.0 26-27 36.48438356145371 39.0 35.0 41.0 29.0 41.0 28-29 36.33487195465535 39.0 35.0 41.0 28.5 41.0 30-31 36.170003164354924 39.0 35.0 40.0 28.0 41.0 32-33 35.956819462448266 38.5 35.0 40.0 27.0 41.0 34-35 35.83856356328571 38.0 35.0 40.0 27.0 41.0 36-37 35.7144246703103 38.0 34.0 40.0 27.0 41.0 38-39 35.5586969797065 38.0 34.0 40.0 26.0 41.0 40-41 35.38482823659382 38.0 34.0 40.0 26.0 41.0 42-43 35.197440342194454 38.0 33.5 40.0 25.0 41.0 44-45 35.0730315769313 38.0 33.0 40.0 24.5 41.0 46-47 34.85120765940438 38.0 33.0 40.0 24.0 41.0 48-49 34.60934865639824 38.0 33.0 40.0 23.5 41.0 50-51 33.971049899656634 37.5 32.0 39.5 23.0 40.5 52-53 34.31949929634739 37.5 33.0 39.5 23.5 40.5 54-55 34.737527028865024 38.0 33.0 40.0 24.0 41.0 56-57 34.657505627833004 38.0 33.0 40.0 24.0 41.0 58-59 34.453528949683985 37.5 33.0 40.0 23.0 41.0 60-61 34.225058498929954 37.0 33.0 40.0 23.0 41.0 62-63 33.91387111637887 36.5 32.0 40.0 23.0 41.0 64-65 33.70159279470831 36.0 32.0 39.0 22.5 41.0 66-67 33.50427069946123 36.0 32.0 39.0 22.0 41.0 68-69 33.15872737416832 35.0 31.5 39.0 22.0 41.0 70-71 32.78168234040132 35.0 31.0 38.5 21.0 40.0 72-73 32.39462059661969 35.0 31.0 37.5 20.0 40.0 74-75 32.03316590934956 35.0 30.5 37.0 20.0 39.0 76-77 31.675165920452557 35.0 30.0 36.5 19.5 39.0 78-79 31.311937737153137 34.5 30.0 36.0 18.5 39.0 80-81 30.959067611716996 34.0 29.0 35.5 18.0 37.5 82-83 30.632829488457045 34.0 29.0 35.0 18.0 37.0 84-85 30.332921990323737 34.0 29.0 35.0 17.0 36.5 86-87 30.0361327002773 34.0 29.0 35.0 16.0 36.0 88-89 29.774333611833576 34.0 28.5 35.0 15.0 36.0 90-91 29.500864021562023 34.0 27.5 35.0 12.5 35.5 92-93 29.23763098070021 34.0 27.0 35.0 11.0 35.0 94-95 28.98704987745064 34.0 26.5 35.0 10.0 35.0 96-97 28.72129714958239 33.0 25.5 35.0 10.0 35.0 98-99 28.429504070637286 33.0 25.0 35.0 10.0 35.0 100 27.167646552657363 32.0 23.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2922386240621542 1101 2 -0.2222430151013981 1101 3 -0.3278664808087868 1101 4 -0.24566250163371706 1101 5 -0.19050304212397862 1101 6 -0.2612354839681288 1101 7 -0.24057961416657747 1101 8 -0.320358037099723 1101 9 -0.45394840298592953 1101 10-11 -0.3899161023213793 1101 12-13 -0.40016680154279527 1101 14-15 -0.6636465742794968 1101 16-17 -0.6518504842149753 1101 18-19 -0.6260881784277785 1101 20-21 -0.704227090102826 1101 22-23 -0.6683492480094699 1101 24-25 -0.7014372926466805 1101 26-27 -0.7986234033607715 1101 28-29 -0.7234874014909991 1101 30-31 -0.7284193230378122 1101 32-33 -0.7089258077069474 1101 34-35 -0.7248257755891103 1101 36-37 -0.711658618666533 1101 38-39 -0.7786690251095294 1101 40-41 -1.0152593769035434 1101 42-43 -1.0316633695757602 1101 44-45 -0.8954553473584497 1101 46-47 -1.010539386946398 1101 48-49 -0.9415432751315862 1101 50-51 -0.9483645812971488 1101 52-53 -0.7131205129929725 1101 54-55 -0.7244343822254109 1101 56-57 -0.6838977951999894 1101 58-59 -0.7275835183961021 1101 60-61 -0.704619783581343 1101 62-63 -0.778368262894304 1101 64-65 -0.6627502275347439 1101 66-67 -0.79173206871166 1101 68-69 -0.850074327112786 1101 70-71 -0.8726310660987124 1101 72-73 -1.0196051785631965 1101 74-75 -1.047243926322004 1101 76-77 -1.0424485514369515 1101 78-79 -1.079774108105358 1101 80-81 -1.168405114732316 1101 82-83 -1.2274951221296035 1101 84-85 -1.2265652543175705 1101 86-87 -1.3022593697751716 1101 88-89 -1.4339625897321184 1101 90-91 -1.5443222033947848 1101 92-93 -1.628887488069541 1101 94-95 -1.6179744729213077 1101 96-97 -1.803343526314297 1101 98-99 -1.8256018495679776 1101 100 -1.972365419408277 1102 1 -0.3852293120323864 1102 2 -0.4737184735525233 1102 3 -0.4888256529925208 1102 4 -0.5187170762097395 1102 5 -0.32958865378855506 1102 6 -0.3314555043805285 1102 7 -0.49748407930442085 1102 8 -0.39747484541882727 1102 9 -0.6345476635631968 1102 10-11 -0.5370552423510588 1102 12-13 -0.6498392396808725 1102 14-15 -0.7819218466706701 1102 16-17 -0.8459058235034291 1102 18-19 -0.7787880799785754 1102 20-21 -0.9232028722019265 1102 22-23 -0.8752134076838374 1102 24-25 -0.8333922619227039 1102 26-27 -0.9308944939189772 1102 28-29 -1.0275753765384863 1102 30-31 -0.8991035148662263 1102 32-33 -0.961239100456325 1102 34-35 -1.0686434594136358 1102 36-37 -1.1325694769809758 1102 38-39 -1.1684368942413457 1102 40-41 -1.2908846949065023 1102 42-43 -1.1948907495926733 1102 44-45 -1.260338149156425 1102 46-47 -1.3882214878583383 1102 48-49 -1.6331079926847423 1102 50-51 -1.2615211350934672 1102 52-53 -1.107071833326195 1102 54-55 -1.0931512459410087 1102 56-57 -1.014699523931803 1102 58-59 -0.9927881993996195 1102 60-61 -1.1141217495546485 1102 62-63 -1.0183494386246181 1102 64-65 -1.1020051522527154 1102 66-67 -1.1145113919712557 1102 68-69 -1.193169372739625 1102 70-71 -1.1482042993570793 1102 72-73 -1.1396838099687905 1102 74-75 -1.2372190806945653 1102 76-77 -1.2503794699047575 1102 78-79 -1.289500858890392 1102 80-81 -1.366706075741014 1102 82-83 -1.471753117740036 1102 84-85 -1.5239600762509689 1102 86-87 -1.5949116574395177 1102 88-89 -1.6208365190594414 1102 90-91 -1.6339526726693023 1102 92-93 -1.8491279058313417 1102 94-95 -1.7958789501018657 1102 96-97 -1.992177445224673 1102 98-99 -1.9277991935470702 1102 100 -1.9669383696628309 1103 1 -0.42299689156030595 1103 2 -0.4641106412567808 1103 3 -0.565645522543921 1103 4 -0.4429067038822936 1103 5 -0.367301365005396 1103 6 -0.39114759427788925 1103 7 -0.5308272007658772 1103 8 -0.5229001433216283 1103 9 -0.6575053569322762 1103 10-11 -0.6445766965331572 1103 12-13 -0.7030018262173883 1103 14-15 -0.9073979282712799 1103 16-17 -0.8444353876353432 1103 18-19 -0.9342258210081198 1103 20-21 -0.857455118988284 1103 22-23 -0.7990893295860317 1103 24-25 -0.8468868527988747 1103 26-27 -0.9426277198568656 1103 28-29 -0.9626388673685327 1103 30-31 -0.9697661038933632 1103 32-33 -1.0534219352177843 1103 34-35 -0.9465278812964044 1103 36-37 -1.0434448469622595 1103 38-39 -1.024969946732611 1103 40-41 -1.0318337149480001 1103 42-43 -1.0696900431538339 1103 44-45 -1.0508202157117097 1103 46-47 -1.1757441026683466 1103 48-49 -1.2069363686923253 1103 50-51 -1.1667875340685256 1103 52-53 -0.9279211688791662 1103 54-55 -0.9652998870100973 1103 56-57 -1.0197184924772884 1103 58-59 -0.9736328575722837 1103 60-61 -1.0103030471067669 1103 62-63 -0.9261813483207888 1103 64-65 -1.0110210269527258 1103 66-67 -1.1873295111164381 1103 68-69 -1.1395501936871142 1103 70-71 -1.1004051338136804 1103 72-73 -1.1157529201308094 1103 74-75 -1.2387852434594286 1103 76-77 -1.185165269298441 1103 78-79 -1.146245850506972 1103 80-81 -1.0771681027851372 1103 82-83 -1.072871567041517 1103 84-85 -1.1915161051899545 1103 86-87 -1.3631404894294121 1103 88-89 -1.3064740599429356 1103 90-91 -1.2978667583086967 1103 92-93 -1.3248545684930946 1103 94-95 -1.3253852498706387 1103 96-97 -1.451785725831627 1103 98-99 -1.5384198355885026 1103 100 -1.4316928395746729 1104 1 -0.5089747422148676 1104 2 -0.39434713327926474 1104 3 -0.4143802274573112 1104 4 -0.17697276137254647 1104 5 -0.2565914344063316 1104 6 -0.3077790087334762 1104 7 -0.3342401285035379 1104 8 -0.3456159365983069 1104 9 -0.5678298769039714 1104 10-11 -0.5437648381933045 1104 12-13 -0.6143241089376232 1104 14-15 -0.7463721518415269 1104 16-17 -0.7273319357505414 1104 18-19 -0.7394254920676744 1104 20-21 -0.8067692468649952 1104 22-23 -0.8315079440409932 1104 24-25 -0.7432827218477271 1104 26-27 -0.774139199901569 1104 28-29 -0.9229706076628261 1104 30-31 -0.8472315355930533 1104 32-33 -0.9019547222613298 1104 34-35 -0.8649977713075501 1104 36-37 -0.930558273182605 1104 38-39 -0.9656087387436258 1104 40-41 -1.0123282731510699 1104 42-43 -0.9583583503610242 1104 44-45 -1.0684193679809724 1104 46-47 -0.962202476642922 1104 48-49 -0.9998306202523608 1104 50-51 -0.905515600967 1104 52-53 -0.9736231895550844 1104 54-55 -0.8679916896005793 1104 56-57 -0.8696049132182893 1104 58-59 -0.825253557859206 1104 60-61 -0.8761124859923584 1104 62-63 -0.8952444606216972 1104 64-65 -0.871074361751738 1104 66-67 -0.9981621920765988 1104 68-69 -1.049449870462464 1104 70-71 -0.9821473935108145 1104 72-73 -1.0270197031744779 1104 74-75 -1.0749010020317407 1104 76-77 -1.0946889820961019 1104 78-79 -1.1418262376430626 1104 80-81 -1.1394566992500152 1104 82-83 -1.1273642146511484 1104 84-85 -1.1173768281176244 1104 86-87 -1.2052195004856543 1104 88-89 -1.1971455006872276 1104 90-91 -1.2105533674364715 1104 92-93 -1.3714601780375553 1104 94-95 -1.2216101209046073 1104 96-97 -1.3552717151382332 1104 98-99 -1.4223790220627315 1104 100 -1.546357731747289 1105 1 -0.4145844736966069 1105 2 -0.4612071674419127 1105 3 -0.42232011933931446 1105 4 -0.25866298873370397 1105 5 -0.27039576890940253 1105 6 -0.2883278010834971 1105 7 -0.33430909851882973 1105 8 -0.36381343148740086 1105 9 -0.44882951855975506 1105 10-11 -0.3854147316833547 1105 12-13 -0.4901519248259092 1105 14-15 -0.6293056246392368 1105 16-17 -0.5192907605577091 1105 18-19 -0.5628027709831755 1105 20-21 -0.6277973166304207 1105 22-23 -0.5916124963993568 1105 24-25 -0.5566933064398327 1105 26-27 -0.6506053398409719 1105 28-29 -0.6353465389454485 1105 30-31 -0.6860101773597975 1105 32-33 -0.6911581199904191 1105 34-35 -0.7606321169320438 1105 36-37 -0.7229175879392642 1105 38-39 -0.7648927695424845 1105 40-41 -0.7157869718255725 1105 42-43 -0.6506577872433397 1105 44-45 -0.7606065645972464 1105 46-47 -0.7850425086577459 1105 48-49 -0.8984775194504309 1105 50-51 -0.7994852718713261 1105 52-53 -0.6855789050890095 1105 54-55 -0.616445111975203 1105 56-57 -0.6685833464069546 1105 58-59 -0.6125592727933835 1105 60-61 -0.5595410997775119 1105 62-63 -0.6252937474754958 1105 64-65 -0.7079243498131831 1105 66-67 -0.6155350197085383 1105 68-69 -0.5618850775374398 1105 70-71 -0.5924905858704363 1105 72-73 -0.6281987038851682 1105 74-75 -0.6096786754069896 1105 76-77 -0.6478063307350297 1105 78-79 -0.6722005714639359 1105 80-81 -0.7106206005876565 1105 82-83 -0.8191689954914274 1105 84-85 -0.7798043065213882 1105 86-87 -0.821266213239781 1105 88-89 -0.9519604230247403 1105 90-91 -0.885374586567707 1105 92-93 -0.9212181317047019 1105 94-95 -0.9634344565246948 1105 96-97 -1.0261808420827947 1105 98-99 -1.027291961943373 1105 100 -1.0132801669061031 1106 1 -0.4951120708203476 1106 2 -0.5272789477413617 1106 3 -0.565559037446306 1106 4 -0.46419608790587574 1106 5 -0.47620626161259594 1106 6 -0.5318209036704431 1106 7 -0.5140927690349955 1106 8 -0.5096159994350486 1106 9 -0.6510810718980693 1106 10-11 -0.8323111384608666 1106 12-13 -0.7182920437496136 1106 14-15 -0.8557876349610964 1106 16-17 -0.8275564264075896 1106 18-19 -0.8979825503874821 1106 20-21 -0.9423194492212374 1106 22-23 -0.8838043255944612 1106 24-25 -1.0009838076447224 1106 26-27 -0.9478635455226438 1106 28-29 -0.9901336100096998 1106 30-31 -1.017773720784625 1106 32-33 -0.9920920890437799 1106 34-35 -0.9081688741143097 1106 36-37 -0.9038963372337037 1106 38-39 -1.1113448410462254 1106 40-41 -1.141479996302614 1106 42-43 -1.0017233291791925 1106 44-45 -1.023006669405902 1106 46-47 -0.9994336349359827 1106 48-49 -0.9816266423212099 1106 50-51 -0.8802917861702682 1106 52-53 -0.8743820299906844 1106 54-55 -0.7836764712721234 1106 56-57 -0.8347823521241722 1106 58-59 -0.8580102468670106 1106 60-61 -0.6861413945473558 1106 62-63 -0.7875382419396075 1106 64-65 -0.796204496828814 1106 66-67 -0.7945523536327883 1106 68-69 -0.8439996369801293 1106 70-71 -0.9632470054573901 1106 72-73 -0.8170521654760847 1106 74-75 -0.893376086715211 1106 76-77 -0.971430578229743 1106 78-79 -1.0043825075809423 1106 80-81 -0.9479211053405017 1106 82-83 -1.0390470127640015 1106 84-85 -1.0443895766698184 1106 86-87 -0.9504613981991241 1106 88-89 -0.9805325436047205 1106 90-91 -1.0947095165597567 1106 92-93 -1.1444811220354048 1106 94-95 -1.1020151767781599 1106 96-97 -1.1839344122519826 1106 98-99 -1.163295071260979 1106 100 -1.1273015487579237 1107 1 -0.6184618179631336 1107 2 -0.6235579641915834 1107 3 -0.5987017766516658 1107 4 -0.4896384345804705 1107 5 -0.4952542082643703 1107 6 -0.5216389222516042 1107 7 -0.5834067670615184 1107 8 -0.5562893791693284 1107 9 -0.6461246060120018 1107 10-11 -0.7125688114538775 1107 12-13 -0.8023817379161571 1107 14-15 -0.9001585935311596 1107 16-17 -0.9022003275206032 1107 18-19 -0.9052783969904183 1107 20-21 -0.8877676573187969 1107 22-23 -0.9768093968689939 1107 24-25 -0.9688745670412189 1107 26-27 -0.9765903247011991 1107 28-29 -0.9759247374032256 1107 30-31 -1.0554451091187786 1107 32-33 -1.0462435520282867 1107 34-35 -1.0611352116010337 1107 36-37 -1.0129273487270183 1107 38-39 -1.0569993735281926 1107 40-41 -1.0182294703244636 1107 42-43 -1.0582504038398781 1107 44-45 -1.1113713959860263 1107 46-47 -1.019727078770579 1107 48-49 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0.5355099767078073 2116 40-41 0.4428913805171888 2116 42-43 0.4601220180629966 2116 44-45 0.35500209161365603 2116 46-47 0.3357985833164676 2116 48-49 0.32394789994091866 2116 50-51 0.3199493357644698 2116 52-53 0.20659105855531834 2116 54-55 0.19794965304598122 2116 56-57 0.2855099196324673 2116 58-59 0.18274343618057998 2116 60-61 0.32147945549601076 2116 62-63 0.1536908207493326 2116 64-65 0.07239554809295612 2116 66-67 0.3018069022339347 2116 68-69 0.2896710330647778 2116 70-71 0.1261279867584335 2116 72-73 0.24979480158241074 2116 74-75 0.4378399565851936 2116 76-77 0.6349549796607477 2116 78-79 0.6740604618643253 2116 80-81 0.7357448340482193 2116 82-83 0.6205026464589167 2116 84-85 0.696471623542589 2116 86-87 0.8043318852173513 2116 88-89 0.8474336061265966 2116 90-91 0.8983509964938854 2116 92-93 1.0355451311603545 2116 94-95 1.1372880736390698 2116 96-97 1.1278265155991782 2116 98-99 1.1927244003604613 2116 100 1.1328611886452968 2201 1 0.9673521531353657 2201 2 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0.7256343057132675 2202 90-91 0.6214815157837528 2202 92-93 0.6548680196268499 2202 94-95 0.5843640773109726 2202 96-97 0.5783840687330688 2202 98-99 0.4802533670998308 2202 100 0.47658807244851786 2203 1 -0.06562479546675704 2203 2 0.1616924847033161 2203 3 0.5942332033850448 2203 4 0.686917509779633 2203 5 0.6915670125042581 2203 6 0.7982521303379642 2203 7 0.8339684953367481 2203 8 0.8626343064947051 2203 9 1.042472108242336 2203 10-11 1.100029019708174 2203 12-13 1.1070295461371842 2203 14-15 1.3082225376727905 2203 16-17 1.3222843085767764 2203 18-19 1.2859727464810007 2203 20-21 1.2585518637086537 2203 22-23 1.3233038843643783 2203 24-25 1.31534336944528 2203 26-27 1.2552516366657969 2203 28-29 1.278631470241848 2203 30-31 1.3007307341790266 2203 32-33 1.2785620102288675 2203 34-35 1.2926374843138575 2203 36-37 1.278150800240752 2203 38-39 1.276277472261107 2203 40-41 1.2179990120467608 2203 42-43 1.2741661736152992 2203 44-45 1.264894505556299 2203 46-47 1.2709253975380719 2203 48-49 1.2887958007760787 2203 50-51 1.4324619632781221 2203 52-53 1.2803409137622168 2203 54-55 1.2222094073141463 2203 56-57 1.2920278825922367 2203 58-59 1.2200130745520426 2203 60-61 1.2397393248806665 2203 62-63 1.1862304814931264 2203 64-65 1.1629441996504042 2203 66-67 1.0873923186732384 2203 68-69 1.0274183510419093 2203 70-71 1.0149979239062645 2203 72-73 0.9181952758336536 2203 74-75 0.933741908085004 2203 76-77 0.8407455188558828 2203 78-79 0.7733127192006677 2203 80-81 0.6800986930594775 2203 82-83 0.7356771035925007 2203 84-85 0.7573834766166065 2203 86-87 0.8132296603338105 2203 88-89 0.742104182087207 2203 90-91 0.6574370325519148 2203 92-93 0.6232532054217437 2203 94-95 0.6369494402800804 2203 96-97 0.6873085966756527 2203 98-99 0.5001551013074312 2203 100 0.4606370996690998 2204 1 0.9643941708560675 2204 2 1.009113129110908 2204 3 1.0447471227869798 2204 4 0.8923822322234471 2204 5 0.9365957975271542 2204 6 0.9431223453818944 2204 7 1.0568384469828302 2204 8 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1.6529614085679114 2205 34-35 1.5961309460881807 2205 36-37 1.658847509706618 2205 38-39 1.5784589317135627 2205 40-41 1.601098520487831 2205 42-43 1.629230350260805 2205 44-45 1.7126271716862576 2205 46-47 1.6642164292394597 2205 48-49 1.6643954100694032 2205 50-51 1.7093052538436453 2205 52-53 1.6505669446452558 2205 54-55 1.5737255465812652 2205 56-57 1.5121119218220542 2205 58-59 1.505820606557748 2205 60-61 1.5219579478085663 2205 62-63 1.4582383107660135 2205 64-65 1.4789885271893226 2205 66-67 1.4482941325274155 2205 68-69 1.3814208789484397 2205 70-71 1.3883915691901336 2205 72-73 1.2150002720861934 2205 74-75 1.3331350314296557 2205 76-77 1.2917070723183315 2205 78-79 1.254000806607305 2205 80-81 1.2963175896039019 2205 82-83 1.2702590615735332 2205 84-85 1.306534937672545 2205 86-87 1.3416032510217626 2205 88-89 1.2528998033735022 2205 90-91 1.2579590733139447 2205 92-93 1.2248911982026591 2205 94-95 1.2564770520555228 2205 96-97 1.3333593566030935 2205 98-99 1.2360534880315264 2205 100 1.2694377666140007 2206 1 1.0287537118514116 2206 2 1.0458186772359035 2206 3 1.0664202783468895 2206 4 1.0567640921746815 2206 5 1.0329466725689755 2206 6 1.0062160741003225 2206 7 1.1028612487075904 2206 8 1.1024707473115996 2206 9 1.3051810038086842 2206 10-11 1.278014850757053 2206 12-13 1.3246902600267845 2206 14-15 1.5476168275961726 2206 16-17 1.5630834290488664 2206 18-19 1.5521865381973114 2206 20-21 1.5673914079039903 2206 22-23 1.5515733386528936 2206 24-25 1.5992488619626641 2206 26-27 1.6132457851283633 2206 28-29 1.6310390329470508 2206 30-31 1.6599096192395564 2206 32-33 1.6480714207529559 2206 34-35 1.668008127811305 2206 36-37 1.6757310192163146 2206 38-39 1.6336505987121015 2206 40-41 1.6812738494117454 2206 42-43 1.6782051737737405 2206 44-45 1.7559590927113078 2206 46-47 1.7719959521888597 2206 48-49 1.7810379816264899 2206 50-51 1.719329349537368 2206 52-53 1.643633140400354 2206 54-55 1.5402310296042074 2206 56-57 1.5777921792871226 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1.5854140482653207 2207 18-19 1.5589483350704896 2207 20-21 1.577726600610056 2207 22-23 1.6339064853662322 2207 24-25 1.6770513176852617 2207 26-27 1.656340678210647 2207 28-29 1.6593509901068941 2207 30-31 1.6628598676858175 2207 32-33 1.7087764869759212 2207 34-35 1.7265980722072314 2207 36-37 1.6895400561431728 2207 38-39 1.7526469473532131 2207 40-41 1.735173484590085 2207 42-43 1.7217199404200514 2207 44-45 1.788516904703414 2207 46-47 1.7536637084957718 2207 48-49 1.7867142517243835 2207 50-51 1.8410424145395368 2207 52-53 1.7516296215008111 2207 54-55 1.583921418434521 2207 56-57 1.531587905149344 2207 58-59 1.5785506217965732 2207 60-61 1.5054350443924065 2207 62-63 1.4791907015603982 2207 64-65 1.5197758788496998 2207 66-67 1.4893322628005095 2207 68-69 1.5210241898885712 2207 70-71 1.5063124336438989 2207 72-73 1.484244709711895 2207 74-75 1.504233018231858 2207 76-77 1.4415710419788184 2207 78-79 1.4384818081566202 2207 80-81 1.4301699288955945 2207 82-83 1.481348917116044 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42-43 1.6198787002515331 2310 44-45 1.741352905000113 2310 46-47 1.6858799314795547 2310 48-49 1.7757881274571332 2310 50-51 1.6888486994007508 2310 52-53 1.5240013121692257 2310 54-55 1.5160626790979919 2310 56-57 1.5232608642016174 2310 58-59 1.5118265675594458 2310 60-61 1.566793111574306 2310 62-63 1.5379836888043599 2310 64-65 1.4964919119495903 2310 66-67 1.4951736776746003 2310 68-69 1.5622398787869471 2310 70-71 1.488702286482905 2310 72-73 1.4546622886970368 2310 74-75 1.4529722516911647 2310 76-77 1.4876428924898732 2310 78-79 1.5765554747720891 2310 80-81 1.5908451715670964 2310 82-83 1.5438647466405797 2310 84-85 1.592143619105041 2310 86-87 1.692123821579635 2310 88-89 1.7187282072353547 2310 90-91 1.7568972031238737 2310 92-93 1.7758511347281285 2310 94-95 1.8809070672944657 2310 96-97 1.8923948936274861 2310 98-99 1.9358493874840441 2310 100 1.9798779356010883 2311 1 0.7484722670171955 2311 2 0.7806410926196605 2311 3 0.8518083570763473 2311 4 0.7179327094159831 2311 5 0.7803939423063966 2311 6 0.7877679677358742 2311 7 0.7958296092777175 2311 8 0.8351081779307918 2311 9 0.9798904816137508 2311 10-11 0.9861578695866768 2311 12-13 1.0384807232704674 2311 14-15 1.2750027413871123 2311 16-17 1.2218401840556936 2311 18-19 1.2364179284768895 2311 20-21 1.2978890921238033 2311 22-23 1.3433925245828604 2311 24-25 1.3471497240645363 2311 26-27 1.3715958330951636 2311 28-29 1.4419980410506597 2311 30-31 1.4499082719844978 2311 32-33 1.5102130798869666 2311 34-35 1.5126770338712916 2311 36-37 1.548687899137576 2311 38-39 1.563487880553616 2311 40-41 1.6423530289913728 2311 42-43 1.6406739062880504 2311 44-45 1.6597340434368348 2311 46-47 1.6773578082171952 2311 48-49 1.6273920521321728 2311 50-51 1.5776228990271477 2311 52-53 1.4346702751606415 2311 54-55 1.398415703072267 2311 56-57 1.368388063274466 2311 58-59 1.2806349918640691 2311 60-61 1.311329594206093 2311 62-63 1.3260452714845883 2311 64-65 1.3767118512878156 2311 66-67 1.408500230550736 2311 68-69 1.4274536945305698 2311 70-71 1.430499819318129 2311 72-73 1.4524323330868256 2311 74-75 1.3925014484439018 2311 76-77 1.404745531962618 2311 78-79 1.4676026233845185 2311 80-81 1.459789502322593 2311 82-83 1.4780666808825913 2311 84-85 1.4677486062808889 2311 86-87 1.50256888454647 2311 88-89 1.5588455422202294 2311 90-91 1.553149016928689 2311 92-93 1.562021050681306 2311 94-95 1.6320584257179078 2311 96-97 1.7738939033165373 2311 98-99 1.8483862140628418 2311 100 1.8851934219683564 2312 1 0.5825715300170664 2312 2 0.6310176078346679 2312 3 0.7051439534007393 2312 4 0.6387449057191148 2312 5 0.6510079815879521 2312 6 0.5462537904812379 2312 7 0.6718238957408929 2312 8 0.7362015850897521 2312 9 0.8891598486080952 2312 10-11 0.9610874346930416 2312 12-13 1.0232938127707314 2312 14-15 1.173216754907692 2312 16-17 1.1609586890416779 2312 18-19 1.1288891428368828 2312 20-21 1.183005604290237 2312 22-23 1.2352902619480233 2312 24-25 1.244647098751173 2312 26-27 1.2337658268461595 2312 28-29 1.2721420662761673 2312 30-31 1.1808911459981388 2312 32-33 1.2929607775701157 2312 34-35 1.2893894637668524 2312 36-37 1.2630340203390347 2312 38-39 1.320150256536742 2312 40-41 1.3526841784539698 2312 42-43 1.3955855765160763 2312 44-45 1.418981319551179 2312 46-47 1.4125988364513375 2312 48-49 1.52524300745376 2312 50-51 1.4114223110244524 2312 52-53 1.243783135793194 2312 54-55 1.2331480926614589 2312 56-57 1.1327311752790266 2312 58-59 1.1658034900160814 2312 60-61 1.1522388103918573 2312 62-63 1.153003175913625 2312 64-65 1.2177282595666554 2312 66-67 1.1250111314367928 2312 68-69 1.2168885870403727 2312 70-71 1.1647632101036862 2312 72-73 1.2408553198637975 2312 74-75 1.2474851706573418 2312 76-77 1.2086041098330327 2312 78-79 1.2064056548550859 2312 80-81 1.0705755186127561 2312 82-83 1.2806893506533257 2312 84-85 1.3387461916113033 2312 86-87 1.4279658313646806 2312 88-89 1.4382901822780951 2312 90-91 1.44727211164302 2312 92-93 1.5219867373997253 2312 94-95 1.6623917209909962 2312 96-97 1.634174093295293 2312 98-99 1.4765132196082007 2312 100 1.600951011191043 2313 1 0.5321736638841656 2313 2 0.5640058706720481 2313 3 0.6283318865074037 2313 4 0.5363373799952029 2313 5 0.5185974176948065 2313 6 0.5502134632525397 2313 7 0.5853008570355058 2313 8 0.6509720022571983 2313 9 0.7854208898959172 2313 10-11 0.6826045981152689 2313 12-13 0.7649176248689074 2313 14-15 1.0042225006218644 2313 16-17 0.9350345047677848 2313 18-19 0.9741379823009098 2313 20-21 1.0072274085159734 2313 22-23 1.0214712832196753 2313 24-25 1.0897401203445085 2313 26-27 1.1079381491244291 2313 28-29 1.1402034238910232 2313 30-31 1.1452591174393518 2313 32-33 1.2124666059403566 2313 34-35 1.1607609737816418 2313 36-37 1.1726211197363128 2313 38-39 1.1880920979353675 2313 40-41 1.2534510927909963 2313 42-43 1.2602960121806035 2313 44-45 1.267666529475271 2313 46-47 1.1596980550163423 2313 48-49 1.2907817910244148 2313 50-51 1.2317274459772491 2313 52-53 1.0884221074622715 2313 54-55 1.0569599674352688 2313 56-57 1.0423836176543375 2313 58-59 1.0574112176888875 2313 60-61 1.0318189622109628 2313 62-63 1.0313150037745231 2313 64-65 1.0390498408443847 2313 66-67 1.0715064021689429 2313 68-69 1.0552602176883568 2313 70-71 1.1244717245729703 2313 72-73 1.1171443078406895 2313 74-75 1.045406705468956 2313 76-77 1.0349269421216292 2313 78-79 1.10792205873501 2313 80-81 1.1770142034429085 2313 82-83 1.2373222772591959 2313 84-85 1.2496267468814146 2313 86-87 1.2932230257941058 2313 88-89 1.329522048205476 2313 90-91 1.4604099596362659 2313 92-93 1.5262440837376126 2313 94-95 1.5390995742116758 2313 96-97 1.592728215152686 2313 98-99 1.6613517498491568 2313 100 1.6837902696990348 2314 1 0.5158344813549647 2314 2 0.4930791223535458 2314 3 0.5923980569080598 2314 4 0.4867513713939715 2314 5 0.5448953278289608 2314 6 0.5654688079335557 2314 7 0.6484919717114721 2314 8 0.6375949977654756 2314 9 0.8434494345016788 2314 10-11 0.7810070316369462 2314 12-13 0.5807096687253335 2314 14-15 0.9496475506378204 2314 16-17 0.9681363240568714 2314 18-19 1.010342226959338 2314 20-21 1.0614058049476327 2314 22-23 0.879293974394308 2314 24-25 0.9759691519633904 2314 26-27 1.0898334384362087 2314 28-29 1.0785893109528146 2314 30-31 1.0118385661869453 2314 32-33 1.0176481186258712 2314 34-35 1.0779047721595063 2314 36-37 1.1831029240549071 2314 38-39 1.2057798024711488 2314 40-41 1.2884010590940917 2314 42-43 1.3075674214824957 2314 44-45 1.341314986195215 2314 46-47 1.3387948170822241 2314 48-49 1.3066805156949428 2314 50-51 1.2871811471063594 2314 52-53 1.0773815892250695 2314 54-55 1.1228655861380972 2314 56-57 1.1201958182164091 2314 58-59 1.1231844193348692 2314 60-61 1.0517464839859088 2314 62-63 1.0901890029271613 2314 64-65 1.0812603106561909 2314 66-67 1.131195636613974 2314 68-69 1.1840319653154978 2314 70-71 1.1899210227534383 2314 72-73 1.1754934764545553 2314 74-75 1.1553070730759174 2314 76-77 1.2082180475186135 2314 78-79 1.2795907520150642 2314 80-81 1.3403663753356128 2314 82-83 1.1811995798620707 2314 84-85 1.2089541926303191 2314 86-87 1.3871308814650156 2314 88-89 1.5235951924407338 2314 90-91 1.5379996004419247 2314 92-93 1.6447296470054766 2314 94-95 1.6220303948990846 2314 96-97 1.7124760761249753 2314 98-99 1.8012923318186616 2314 100 1.7563226612191478 2315 1 0.4233735630799238 2315 2 0.4227100978760454 2315 3 0.510275874410894 2315 4 0.40933773061865253 2315 5 0.3759594144953624 2315 6 -0.3514914432843952 2315 7 0.26451052985999723 2315 8 0.32896271073570915 2315 9 0.5422717434448856 2315 10-11 0.6456042365348367 2315 12-13 0.6593251808043021 2315 14-15 0.7532870482080369 2315 16-17 0.7106642573591131 2315 18-19 0.7676180296350807 2315 20-21 0.7899709822455137 2315 22-23 0.6902403209774448 2315 24-25 0.8376432277448558 2315 26-27 0.8774949939516432 2315 28-29 0.947098927145241 2315 30-31 1.0189894770475263 2315 32-33 1.037154879777276 2315 34-35 0.9350964327696474 2315 36-37 1.0581425625518222 2315 38-39 1.1292270770075348 2315 40-41 1.1873161137188362 2315 42-43 1.2800980194998672 2315 44-45 1.1778357052910167 2315 46-47 1.2049613513150703 2315 48-49 1.260355619175094 2315 50-51 1.199092072453702 2315 52-53 0.9825845009056167 2315 54-55 0.8524013147058938 2315 56-57 0.8451387510008175 2315 58-59 0.8529552866740531 2315 60-61 0.7362488703914423 2315 62-63 0.8949142630824838 2315 64-65 0.9898749016556394 2315 66-67 0.9754931003917235 2315 68-69 1.0330997198030474 2315 70-71 1.0320742271478593 2315 72-73 1.115448714739209 2315 74-75 1.1029089516504484 2315 76-77 1.0674664420449318 2315 78-79 1.0549286110107765 2315 80-81 1.0833388478888182 2315 82-83 1.274529451891592 2315 84-85 1.25223039993951 2315 86-87 1.3219234728390035 2315 88-89 1.5277708484158516 2315 90-91 1.5506722150216241 2315 92-93 1.6907755083645277 2315 94-95 1.6934067641838482 2315 96-97 1.7255898503728098 2315 98-99 1.858791102948885 2315 100 1.9162152691511842 2316 1 -0.08400177954827015 2316 2 -0.17197454736410123 2316 3 -0.002722257191777544 2316 4 -0.11968707695466207 2316 5 -0.10978025953087212 2316 6 0.033653733772474936 2316 7 0.014686157688629464 2316 8 0.005195982194535986 2316 9 0.13081085892826394 2316 10-11 0.01687730455270753 2316 12-13 0.12129538982460275 2316 14-15 0.2993310133150686 2316 16-17 0.34824144846924554 2316 18-19 0.2783624164617393 2316 20-21 0.2551599389379149 2316 22-23 0.14013593624873266 2316 24-25 0.1770944411389408 2316 26-27 0.25827879727280134 2316 28-29 0.38997589036868163 2316 30-31 0.33823619290974705 2316 32-33 0.534410395654092 2316 34-35 0.46008848942054925 2316 36-37 0.5115624973935553 2316 38-39 0.5868591342361285 2316 40-41 0.6270869233931506 2316 42-43 0.6434044594656356 2316 44-45 0.45155846036374214 2316 46-47 0.40288102999329567 2316 48-49 0.5905092374656249 2316 50-51 0.4177139051857708 2316 52-53 0.12256959097331332 2316 54-55 0.11415887761037169 2316 56-57 0.2439408255451596 2316 58-59 0.20466095936550488 2316 60-61 0.29968293603315743 2316 62-63 0.3002674722715284 2316 64-65 0.32809559905889074 2316 66-67 0.34141370034641483 2316 68-69 0.35670598509115337 2316 70-71 0.2389922761093004 2316 72-73 0.4053050959469928 2316 74-75 0.5452853888645421 2316 76-77 0.5506883024456997 2316 78-79 0.627321178164383 2316 80-81 0.785343403342516 2316 82-83 0.8506304608869613 2316 84-85 0.8605977300468517 2316 86-87 0.9039991535726202 2316 88-89 1.0533606895944736 2316 90-91 1.0776048622624295 2316 92-93 1.181296223402768 2316 94-95 1.2010886668781993 2316 96-97 1.237826332616958 2316 98-99 1.4102479283761191 2316 100 1.31466726623837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 45.0 1 438.0 2 201.0 3 141.0 4 134.0 5 113.0 6 94.0 7 468.0 8 1552.0 9 3402.0 10 6310.0 11 11475.0 12 17887.0 13 23182.0 14 27481.0 15 32171.0 16 37459.0 17 44660.0 18 52805.0 19 60834.0 20 70337.0 21 79718.0 22 88407.0 23 92512.0 24 96116.0 25 102235.0 26 113570.0 27 128644.0 28 149989.0 29 173797.0 30 203774.0 31 246654.0 32 302762.0 33 383286.0 34 515903.0 35 659796.0 36 921974.0 37 1255492.0 38 1050658.0 39 248495.0 40 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.94739408864391 18.296796891061692 11.442844270155367 24.31296475013903 2 16.566721778478318 20.074960403588037 39.81231404975908 23.54600376817456 3 18.48522759359411 26.735885815474646 30.17982374674082 24.59906284419042 4 12.445957456732648 15.870419502292194 37.661364120970894 34.02225892000427 5 12.551686874959305 38.20954790981991 34.14792611815447 15.090839097066302 6 30.358231219380915 36.338106481611995 19.166534628249483 14.137127670757613 7 27.516676276912854 33.059177451807116 22.61703391139242 16.80711235988761 8 27.15397285170671 35.18985489073236 20.305090926411104 17.351081331149828 9 28.91594837491046 14.159272006983464 19.94789894194198 36.9768806761641 10-11 25.915105877962002 25.25989849061951 28.837754597007816 19.987241034410676 12-13 25.318337961938642 23.545828468744713 28.321767431831134 22.81406613748551 14-15 23.294059278668936 24.96717676295279 26.106848972019804 25.631914986358474 16-17 22.495849971678403 27.245778360828872 27.135747171328063 23.122624496164665 18-19 22.23723185246978 27.103961212086215 28.527795420629136 22.131011514814872 20-21 23.277323733009645 26.81836392293569 27.84818965285079 22.05612269120387 22-23 23.04344901741838 26.370115819669238 27.97643445005422 22.610000712858163 24-25 22.377236353141626 25.857596231111117 28.521226549166297 23.243940866580964 26-27 22.415537753674432 26.235156175657682 28.069862616172266 23.27944345449562 28-29 22.214079986385084 27.65826281966614 27.861452399812965 22.266204794135813 30-31 23.732897462859047 26.375445500574337 28.245572756707233 21.646084279859384 32-33 23.61679332564946 26.261524509788607 27.78874829550917 22.332933869052763 34-35 23.32880209003597 26.71196382890614 28.115601123426266 21.843632957631627 36-37 22.742748337351564 26.541834243387548 28.201699276884845 22.513718142376042 38-39 22.96832144506879 26.860731175989 27.967570077597347 22.20337730134486 40-41 22.87859194953519 26.117796578993218 28.501353867981038 22.50225760349056 42-43 22.575900708866378 26.492543814681135 27.68665383540242 23.244901641050067 44-45 21.60102078786162 26.95127098640986 28.620318843477342 22.827389382251177 46-47 21.67760280845731 26.197149637439026 28.214975060264834 23.91027249383883 48-49 22.278026426408914 26.434871529997856 28.164716238572176 23.122385805021057 50-51 22.06872098742671 26.740851577377434 28.133101082349377 23.05732635284648 52-53 21.782600814448042 27.05476235222014 27.73739385929475 23.425242974037065 54-55 21.68108343248201 26.431812624763147 28.45480660142038 23.432297341334465 56-57 22.304737195991247 26.37860286580415 27.81340025000026 23.503259688204338 58-59 21.604404492665832 26.641598641994378 28.43022951058686 23.323767354752935 60-61 22.43548595329751 26.7074479382853 27.93680684447912 22.920259263938075 62-63 22.404550424755964 26.50327697245658 27.744591543001775 23.347581059785686 64-65 21.857103331778035 26.114522572959647 27.796591604634024 24.231782490628294 66-67 21.802880408679755 25.94130990129464 27.693339968622364 24.562469721403243 68-69 21.281818425416173 26.20116243698306 28.12527984581181 24.391739291788955 70-71 21.570369932243164 25.828029178716516 28.399070695172274 24.20253019386805 72-73 21.617392535880384 26.34258555370655 27.8748287815075 24.165193128905567 74-75 21.89707937138149 26.196220820263722 27.79551087921493 24.11118892913986 76-77 21.637586333504306 25.815902369482064 27.970570087431867 24.57594120958176 78-79 21.28772959526103 25.742258354367 28.44649869439266 24.523513355979315 80-81 21.507163508739783 25.871734619617214 28.04978276780778 24.57131910383522 82-83 22.091933239106044 25.78378141268193 28.248460755334154 23.875824592877873 84-85 22.006315584920326 26.069582477374937 27.69129976125701 24.232802176447727 86-87 21.44615429887794 26.30099557872212 28.05293318445829 24.199916937941648 88-89 21.43437239050289 25.463520952567826 28.27624433558451 24.825862321344776 90-91 22.35307231658111 25.00180287030835 28.22990202172037 24.41522279139017 92-93 22.349221887783653 25.235962892264492 28.042947168993614 24.37186805095824 94-95 21.998862218648647 25.03348722626648 28.125903641469492 24.84174691361538 96-97 21.931619270978157 24.837337974823946 28.162238754771508 25.068803999426386 98-99 21.803050949839132 25.01938245341148 27.922005937635596 25.255560659113783 100 22.09213602883909 24.859509965428302 27.88297157213062 25.16538243360199 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 418.0 1 375.0 2 738.0 3 1188.5 4 1073.5 5 1015.0 6 1442.0 7 2450.0 8 3831.5 9 4855.0 10 4917.5 11 4817.5 12 5210.5 13 5851.0 14 6576.0 15 7391.5 16 8108.5 17 8786.0 18 9537.5 19 10306.5 20 11251.5 21 12415.0 22 14300.5 23 17043.0 24 20274.0 25 24113.0 26 29067.5 27 34588.0 28 41029.5 29 50815.5 30 64151.0 31 76655.0 32 91709.5 33 108352.5 34 123700.5 35 139443.0 36 155633.0 37 176443.5 38 189284.0 39 191265.0 40 192335.5 41 193653.5 42 199576.0 43 210774.5 44 225163.5 45 246876.5 46 269164.0 47 289059.0 48 312497.0 49 333681.0 50 347453.5 51 351848.0 52 339550.0 53 317277.0 54 293461.0 55 260348.5 56 228305.5 57 197301.5 58 164337.0 59 135503.0 60 106181.0 61 81171.5 62 63150.0 63 49064.0 64 36747.0 65 25950.0 66 18627.0 67 14002.0 68 10569.0 69 7963.0 70 6026.5 71 4457.5 72 3303.0 73 2524.0 74 1971.5 75 1442.5 76 1032.0 77 732.5 78 522.5 79 374.5 80 259.0 81 176.5 82 124.0 83 96.0 84 81.5 85 61.5 86 50.5 87 46.0 88 38.5 89 32.0 90 26.5 91 20.5 92 14.5 93 13.0 94 11.0 95 6.5 96 3.0 97 1.0 98 3.0 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22458592750296313 2 0.14982110291648046 3 0.024454356955890558 4 0.023260784482447543 5 0.028159983123440372 6 0.056528147492248715 7 0.06400879357580434 8 0.05547336251571769 9 0.04306576029178683 10-11 0.04532105711660648 12-13 0.04339191090952998 14-15 0.02112345703000308 16-17 0.019340037694684162 18-19 0.01896531145302182 20-21 0.02240030200159328 22-23 0.02586305004954714 24-25 0.022920755115013198 26-27 0.020325428922759205 28-29 0.019381673943757755 30-31 0.0178341933531892 32-33 0.02584917129985594 34-35 0.026910895651232572 36-37 0.02557853568087758 38-39 0.023975540091544234 40-41 0.02532871818643602 42-43 0.031650488670776626 44-45 0.0413586740797695 46-47 0.0457859952312616 48-49 0.041566855325137465 50-51 0.02960337309132495 52-53 0.04447445338544341 54-55 0.04320454778869881 56-57 0.04534881461598888 58-59 0.04888789578724431 60-61 0.024842961947244096 62-63 0.02621001879182708 64-65 0.026772108154320594 66-67 0.03273303114669006 68-69 0.03654274793692386 70-71 0.040067950358488105 72-73 0.044481392760289014 74-75 0.025606293180259976 76-77 0.018944493328485024 78-79 0.018500373338366693 80-81 0.02168554639249659 82-83 0.019458007067059344 84-85 0.011935724734430126 86-87 0.013989779688727402 88-89 0.019506582690978533 90-91 0.020394822671215194 92-93 0.020526670793281576 94-95 0.024523750704346547 96-97 0.025356475685818416 98-99 0.03087327868806955 100 0.031338216802724674 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7205260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.8671948507486 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59038980183067 43.90927010457657 2 14.9091806274021 17.55323282116571 3 4.915708833136039 8.681219691292958 4 2.1289999887058784 5.0131302868956205 5 1.0890124623813229 3.205355440894743 6 0.6281687613319946 2.2187119723490354 7 0.36976312092782776 1.5236842381795548 8 0.2740657467961169 1.2906785374850354 9 0.1892655705553614 1.0027379898378508 >10 0.8376310173820444 8.307435231757857 >50 0.0414594168592716 1.6727463128957947 >100 0.022790597001748306 2.6071642657097724 >500 0.0023665501070297855 0.9354018564334755 >1k 0.0010549453943674305 1.0959770927145662 >5k 5.702407529858189E-5 0.19300193849090044 >10k+ 8.553611294787285E-5 0.7902522193205552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19169 0.26604175283057097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16797 0.23312135856304977 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11117 0.15429006031704615 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.387874969119782E-5 2 0.0 1.387874969119782E-5 0.0 0.0 1.387874969119782E-5 3 0.0 1.387874969119782E-5 0.0 0.0 1.387874969119782E-5 4 0.0 1.387874969119782E-5 0.0 0.0 1.387874969119782E-5 5 0.0 1.387874969119782E-5 0.0 1.387874969119782E-5 1.387874969119782E-5 6 0.0 1.387874969119782E-5 0.0 1.387874969119782E-5 1.387874969119782E-5 7 0.0 1.387874969119782E-5 0.0 1.387874969119782E-5 1.387874969119782E-5 8 0.0 1.387874969119782E-5 0.0 2.775749938239564E-5 1.387874969119782E-5 9 0.0 1.387874969119782E-5 0.0 4.1636249073593455E-5 4.1636249073593455E-5 10-11 0.0 1.387874969119782E-5 0.0 6.93937484559891E-5 4.1636249073593455E-5 12-13 0.0 1.387874969119782E-5 0.0 9.715124783838474E-5 9.715124783838474E-5 14-15 0.0 3.469687422799455E-5 0.0 1.1102999752958256E-4 1.0409062268398365E-4 16-17 0.0 5.551499876479128E-5 0.0 1.387874969119782E-4 1.1102999752958256E-4 18-19 0.0 5.551499876479128E-5 0.0 1.8736312083117055E-4 1.2490874722078039E-4 20-21 0.0 5.551499876479128E-5 0.0 3.4002936743434656E-4 1.457268717575771E-4 22-23 0.0 5.551499876479128E-5 0.0 7.425131084790833E-4 1.52666246603176E-4 24-25 0.0 5.551499876479128E-5 0.0 0.001471147467266969 1.52666246603176E-4 26-27 0.0 5.551499876479128E-5 0.0 0.003296203051659482 1.5960562144877493E-4 28-29 0.0 5.551499876479128E-5 0.0 0.014239597183168962 1.6654499629437382E-4 30-31 0.0 5.551499876479128E-5 0.0 0.057263721225882205 1.6654499629437382E-4 32-33 0.0 5.551499876479128E-5 0.0 0.15121591726044584 1.6654499629437382E-4 34-35 0.0 6.93937484559891E-5 0.0 0.2855483355215495 1.6654499629437382E-4 36-37 0.0 6.93937484559891E-5 0.0 0.5107241098863886 1.7348437113997274E-4 38-39 0.0 7.6333123301588E-5 0.0 0.9088429841532436 1.8736312083117055E-4 40-41 0.0 9.021187299278582E-5 0.0 1.413238384180446 1.9430249567676947E-4 42-43 0.0 9.715124783838474E-5 0.0 1.8480387938811367 1.9430249567676947E-4 44-45 0.0 9.715124783838474E-5 0.0 2.3184034996655223 1.9430249567676947E-4 46-47 0.0 9.715124783838474E-5 0.0 2.8436655998534404 1.9430249567676947E-4 48-49 0.0 9.715124783838474E-5 0.0 3.4336720673507966 2.3593874475036293E-4 50-51 0.0 9.715124783838474E-5 0.0 4.081344739815079 2.3593874475036293E-4 52-53 0.0 9.715124783838474E-5 0.0 4.731161679106653 2.636962441327586E-4 54-55 0.0 9.715124783838474E-5 0.0 5.409603539636321 2.9145374351515423E-4 56-57 0.0 9.715124783838474E-5 0.0 6.099155339293794 2.9839311836075315E-4 58-59 0.0 9.715124783838474E-5 0.0 6.907412085060081 3.05332493206352E-4 60-61 0.0 1.2490874722078039E-4 0.0 7.703427773598732 3.05332493206352E-4 62-63 0.0 1.2490874722078039E-4 0.0 8.533904397620628 3.1921124289754986E-4 64-65 0.0 1.2490874722078039E-4 0.0 9.293564701343186 3.1921124289754986E-4 66-67 0.0 1.52666246603176E-4 0.0 10.124200930986529 3.261506177431487E-4 68-69 0.0 1.52666246603176E-4 0.0 11.086206465831907 3.3308999258874764E-4 70-71 0.0 1.52666246603176E-4 0.0 12.046317551344433 3.469687422799455E-4 72-73 0.0 1.52666246603176E-4 0.0 12.90222698417545 4.024837410447368E-4 74-75 0.0 1.5960562144877493E-4 0.0 13.701719299511746 4.024837410447368E-4 76-77 0.0 1.6654499629437382E-4 0.0 14.555102799898963 4.163624907359346E-4 78-79 0.0 1.6654499629437382E-4 0.0 15.489336956612252 4.302412404271324E-4 80-81 0.0 1.6654499629437382E-4 0.0 16.377445088726848 4.302412404271324E-4 82-83 0.0 1.7348437113997274E-4 0.0 17.27000136011747 4.4411999011833024E-4 84-85 0.0 1.8042374598557166E-4 0.0 18.124980916719174 4.4411999011833024E-4 86-87 0.0 1.8042374598557166E-4 0.0 18.959107651909854 4.4411999011833024E-4 88 0.0 1.8042374598557166E-4 0.0 19.619791652209635 4.4411999011833024E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 7180 0.0 52.311775 2 GGTATCA 10815 0.0 51.184704 1 ACCTGGG 8230 0.0 44.38058 3 GTATCAA 18210 0.0 39.660763 1 CCTGGGG 8120 0.0 35.66075 4 GTACATG 27795 0.0 35.306236 1 TACATGG 28545 0.0 33.901123 2 TGGGGGG 19670 0.0 32.836754 6 ACATGGG 28995 0.0 32.668087 3 CATGGGG 22535 0.0 31.623316 4 CAACGCA 22195 0.0 31.472805 5 ATCAACG 22575 0.0 31.088795 3 ATGGGGG 18360 0.0 30.90341 5 CTGGGGG 6750 0.0 30.642254 5 TATCAAC 23510 0.0 30.3306 2 AACGCAG 23985 0.0 29.594776 6 GTACCTG 12820 0.0 29.582624 1 TCAACGC 24135 0.0 29.137352 4 ACGCAGA 26955 0.0 25.880663 7 CGCAGAG 27355 0.0 25.277943 8 >>END_MODULE