FastQCFastQC Report
Mon 6 Jun 2016
ERR1211404_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211404_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7205260
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176990.2456399907845102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166410.23095627361122292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112340.1559138740309163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG72700.052.938412
GGTATCA112600.049.7316631
ACCTGGG83350.045.5543823
GTATCAA187150.037.8099631
CCTGGGG79950.036.2662934
TGGGGGG180050.034.734276
GTACATG293550.034.2600751
TACATGG297900.033.410032
ACATGGG291000.032.9428833
CTGGGGG62200.032.2562335
ATGGGGG176850.032.2013245
CATGGGG221500.031.8027294
GTACCTG131050.029.993621
CAACGCA230750.029.7702085
ATCAACG232750.029.4772913
TATCAAC246500.028.5191152
AACGCAG247300.028.2527056
TCAACGC246950.028.0875454
GTACTAG29850.028.0372431
ACGCAGA277250.024.6244897
Produced by FastQC (version 0.11.5)