##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211404_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7205260 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0597166514463 33.0 31.0 34.0 30.0 34.0 2 32.184368780585295 33.0 31.0 34.0 30.0 34.0 3 32.26093631596917 34.0 31.0 34.0 30.0 34.0 4 35.91349694528719 37.0 35.0 37.0 35.0 37.0 5 35.77383189503224 37.0 35.0 37.0 35.0 37.0 6 35.811730458026496 37.0 35.0 37.0 35.0 37.0 7 35.7339908622312 37.0 35.0 37.0 33.0 37.0 8 35.71271196320466 37.0 35.0 37.0 33.0 37.0 9 37.29819034982776 39.0 37.0 39.0 34.0 39.0 10-11 37.2677214979057 39.0 37.0 39.0 34.0 39.0 12-13 37.171275012976636 39.0 37.0 39.0 33.0 39.0 14-15 38.37269196392636 40.0 38.0 41.0 33.0 41.0 16-17 38.35055133333148 40.0 38.0 41.0 33.0 41.0 18-19 38.349146179319 40.0 38.0 41.0 33.5 41.0 20-21 38.30394531217472 40.0 38.0 41.0 34.0 41.0 22-23 38.21111313124023 40.0 38.0 41.0 33.5 41.0 24-25 38.097217727049404 40.0 37.0 41.0 33.0 41.0 26-27 37.97811182386201 40.0 37.0 41.0 33.0 41.0 28-29 37.894296527814404 40.0 37.0 41.0 33.0 41.0 30-31 37.95081641745058 40.0 37.0 41.0 33.0 41.0 32-33 37.78987899118144 40.0 37.0 41.0 33.0 41.0 34-35 37.69083523703517 40.0 37.0 41.0 33.0 41.0 36-37 37.56931116989533 40.0 36.5 41.0 32.0 41.0 38-39 37.46383919525458 40.0 36.0 41.0 32.0 41.0 40-41 37.39727490749813 40.0 36.0 41.0 32.0 41.0 42-43 37.27434547816456 40.0 36.0 41.0 31.5 41.0 44-45 37.13703912141963 39.0 36.0 41.0 31.0 41.0 46-47 36.932170178452964 39.0 35.5 41.0 31.0 41.0 48-49 36.72340540105423 39.0 35.0 41.0 30.0 41.0 50-51 36.547789947899176 39.0 35.0 41.0 30.0 41.0 52-53 36.34907525890807 39.0 35.0 40.0 29.5 41.0 54-55 36.15268616260899 38.5 35.0 40.0 29.0 41.0 56-57 35.92750830643169 38.0 35.0 40.0 28.5 41.0 58-59 35.665458290193556 38.0 34.0 40.0 28.0 41.0 60-61 35.40210013240328 37.5 34.0 40.0 28.0 41.0 62-63 35.08283469298818 37.0 34.0 40.0 27.5 41.0 64-65 34.73216809664051 36.5 34.0 39.5 26.5 41.0 66-67 34.38834025420318 36.0 33.0 39.0 26.0 40.5 68-69 34.0277998989627 35.5 33.0 39.0 26.0 40.0 70-71 33.65839476993197 35.0 33.0 38.0 26.0 40.0 72-73 33.30108330580715 35.0 32.0 37.0 26.0 39.5 74-75 32.92946812467558 35.0 32.0 37.0 25.0 39.0 76-77 31.922638947102534 34.0 30.5 36.0 24.5 38.0 78-79 32.40779826127024 34.5 31.5 36.0 25.0 38.0 80-81 32.30774663232138 35.0 32.0 35.5 25.0 37.0 82-83 32.0299912841452 34.5 31.5 35.0 24.5 37.0 84-85 31.75253162273117 34.0 31.0 35.0 24.0 36.5 86-87 31.501044028945522 34.0 31.0 35.0 23.5 36.0 88-89 31.272709381757217 34.0 31.0 35.0 23.0 36.0 90-91 31.045667193133905 34.0 31.0 35.0 22.0 35.0 92-93 30.842453152280417 34.0 30.0 35.0 20.0 35.0 94-95 30.6556449038619 34.0 30.0 35.0 20.0 35.0 96-97 30.475181881014706 34.0 30.0 35.0 20.0 35.0 98-99 30.268642838703947 34.0 29.0 35.0 19.0 35.0 100 28.453629709406737 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2691390332522481 1101 2 -0.20957203469365382 1101 3 -0.2827770867442787 1101 4 -0.1613836682044152 1101 5 -0.20311714709312412 1101 6 -0.22678028325796618 1101 7 -0.198247464434246 1101 8 -0.271200093838992 1101 9 -0.4709024991433921 1101 10-11 -0.3653836086747049 1101 12-13 -0.43523655274939443 1101 14-15 -0.5788320749548959 1101 16-17 -0.5736750369901529 1101 18-19 -0.5239797054918967 1101 20-21 -0.6627275335856808 1101 22-23 -0.6843476213198088 1101 24-25 -0.7493000381414348 1101 26-27 -0.660149566959511 1101 28-29 -0.6648701659422471 1101 30-31 -0.7301137622192968 1101 32-33 -0.5616077183225983 1101 34-35 -0.7499150060096085 1101 36-37 -0.722516369780358 1101 38-39 -0.770635149900933 1101 40-41 -0.7765294442550541 1101 42-43 -0.661712776308498 1101 44-45 -0.680682363555853 1101 46-47 -0.8121772687570257 1101 48-49 -0.7134102340696202 1101 50-51 -0.8299763726883 1101 52-53 -0.7909241230728625 1101 54-55 -0.8471247817999839 1101 56-57 -0.7927225957894279 1101 58-59 -0.7722578633213502 1101 60-61 -0.8554356868397406 1101 62-63 -0.9199040800594815 1101 64-65 -0.8532151481124686 1101 66-67 -0.8358944607045515 1101 68-69 -0.8716742680457443 1101 70-71 -0.8996481958952174 1101 72-73 -0.8322964767934664 1101 74-75 -0.9039434842981535 1101 76-77 -0.8549736051853074 1101 78-79 -0.7865073914373255 1101 80-81 -0.6895101312488023 1101 82-83 -0.6869390842437504 1101 84-85 -0.8351169781949039 1101 86-87 -0.8951223765964507 1101 88-89 -0.982104742468028 1101 90-91 -1.1104826578757354 1101 92-93 -1.1890753736788007 1101 94-95 -1.369273704894006 1101 96-97 -1.257232225025568 1101 98-99 -1.6144834986848444 1101 100 -1.5308015430290531 1102 1 -0.2847150814953636 1102 2 -0.25820292847389226 1102 3 -0.2771626975056485 1102 4 -0.16286891136189752 1102 5 -0.19741627610372348 1102 6 -0.3339155921987782 1102 7 -0.3011933384493801 1102 8 -0.34190972590392477 1102 9 -0.48552634492437363 1102 10-11 -0.46084952628446985 1102 12-13 -0.4704555118974909 1102 14-15 -0.6056144721176295 1102 16-17 -0.6893755015379028 1102 18-19 -0.7261739258916506 1102 20-21 -0.7174143326620097 1102 22-23 -0.6994158809366695 1102 24-25 -0.7519699834373981 1102 26-27 -0.7473429669492688 1102 28-29 -0.801222081185621 1102 30-31 -0.7340843584240275 1102 32-33 -0.6983713815360204 1102 34-35 -0.7069970259342782 1102 36-37 -0.732171955643409 1102 38-39 -0.7563846283008218 1102 40-41 -0.817169243623411 1102 42-43 -0.754692136599445 1102 44-45 -0.7272561733592227 1102 46-47 -0.7822776523175179 1102 48-49 -0.9412061010096622 1102 50-51 -0.9349348855333659 1102 52-53 -0.9527490912349279 1102 54-55 -0.8938719690732384 1102 56-57 -0.8281319803765612 1102 58-59 -0.9140181621011223 1102 60-61 -0.9787049980867337 1102 62-63 -0.9664035637280577 1102 64-65 -0.9313595763996005 1102 66-67 -0.9534543957943171 1102 68-69 -0.938666690167004 1102 70-71 -0.9025599739720676 1102 72-73 -0.9677965957152779 1102 74-75 -1.0413407825149292 1102 76-77 -0.8832153913006557 1102 78-79 -0.7878872984675596 1102 80-81 -0.7151244226403648 1102 82-83 -0.7737095967516154 1102 84-85 -0.8184474513833031 1102 86-87 -0.8941225301410718 1102 88-89 -0.9576763198530109 1102 90-91 -1.0201347061911115 1102 92-93 -1.0720724141814593 1102 94-95 -1.1999114645274247 1102 96-97 -1.2470963229810188 1102 98-99 -1.361277539648139 1102 100 -1.3093776274003162 1103 1 -0.3336346016628937 1103 2 -0.2861889052991913 1103 3 -0.3213771666005698 1103 4 -0.21371385346499494 1103 5 -0.16202793398847604 1103 6 -0.31515655748255256 1103 7 -0.3168316539150169 1103 8 -0.3276168920092033 1103 9 -0.4091445501453066 1103 10-11 -0.4728466331827832 1103 12-13 -0.44492497293775557 1103 14-15 -0.6127405282641192 1103 16-17 -0.7987002502096558 1103 18-19 -0.7088141078263064 1103 20-21 -0.7362865659591762 1103 22-23 -0.8010037618891275 1103 24-25 -0.7689201073207244 1103 26-27 -0.7557319904975657 1103 28-29 -0.7953205871137854 1103 30-31 -0.7484589717556105 1103 32-33 -0.7093334001120439 1103 34-35 -0.6772239311822119 1103 36-37 -0.6614643985111286 1103 38-39 -0.7360059198953834 1103 40-41 -0.8717657286642648 1103 42-43 -0.8449335098464203 1103 44-45 -0.754566657266345 1103 46-47 -0.7627419308801748 1103 48-49 -0.808719552836628 1103 50-51 -0.7908050476327375 1103 52-53 -0.8316542305750332 1103 54-55 -0.8006425393350654 1103 56-57 -0.8647311488090708 1103 58-59 -0.8938177321208585 1103 60-61 -0.9255737186164978 1103 62-63 -0.8956835161472938 1103 64-65 -0.8615507960750861 1103 66-67 -0.9780815752094654 1103 68-69 -0.9380483195690417 1103 70-71 -0.9037170652093707 1103 72-73 -0.9740221440746097 1103 74-75 -0.971538178922664 1103 76-77 -0.9500290666854845 1103 78-79 -0.8308019016573454 1103 80-81 -0.7118359976373512 1103 82-83 -0.7444881613985892 1103 84-85 -0.7020320880569528 1103 86-87 -0.7966065727525553 1103 88-89 -0.8635760206799965 1103 90-91 -1.1365848011832753 1103 92-93 -0.8989062810051358 1103 94-95 -0.9245315581348059 1103 96-97 -1.0604121124022647 1103 98-99 -1.219155824648542 1103 100 -1.2073389819776246 1104 1 -0.41954851551959393 1104 2 -0.3723620045962086 1104 3 -0.372137253691168 1104 4 -0.24082903002224043 1104 5 -0.25460792960311807 1104 6 -0.3303910236205354 1104 7 -0.35967267357965227 1104 8 -0.44167445775632785 1104 9 -0.46210428742212883 1104 10-11 -0.47179298060179065 1104 12-13 -0.6178332886425437 1104 14-15 -0.725939897343018 1104 16-17 -0.6678459487603803 1104 18-19 -0.7274990761534497 1104 20-21 -0.683484793051754 1104 22-23 -0.7988515278369377 1104 24-25 -0.8475989336186345 1104 26-27 -0.8563984669957492 1104 28-29 -0.8700789684427974 1104 30-31 -0.777518343924541 1104 32-33 -0.7353036398004207 1104 34-35 -0.8501562414747568 1104 36-37 -0.8171350291942403 1104 38-39 -0.8819856754861277 1104 40-41 -0.9892168704164845 1104 42-43 -0.8152470596096393 1104 44-45 -0.8402130582927683 1104 46-47 -0.8636804313862072 1104 48-49 -0.8545790136099569 1104 50-51 -0.8324307804430546 1104 52-53 -0.7570875133311574 1104 54-55 -0.7671665170485724 1104 56-57 -0.8632161734552213 1104 58-59 -1.0092123987092378 1104 60-61 -1.0130676053612646 1104 62-63 -0.9881664482898174 1104 64-65 -1.0149088142469864 1104 66-67 -1.0270443359994132 1104 68-69 -0.8766871846367437 1104 70-71 -0.9541876810080296 1104 72-73 -0.932930437150354 1104 74-75 -0.8248533262767452 1104 76-77 -0.8263372350283902 1104 78-79 -0.7975361002459209 1104 80-81 -0.7793647292054118 1104 82-83 -0.870789270358209 1104 84-85 -0.8560319736437343 1104 86-87 -0.6915120218844173 1104 88-89 -0.831313797778467 1104 90-91 -0.7448873357725461 1104 92-93 -1.1093765510281308 1104 94-95 -1.1485291915466647 1104 96-97 -1.108723410380712 1104 98-99 -1.1959897573263625 1104 100 -1.193074028862661 1105 1 -0.3820223846924691 1105 2 -0.3561363868085401 1105 3 -0.37272905109474763 1105 4 -0.23282928135047598 1105 5 -0.2403411633297594 1105 6 -0.27147437200981983 1105 7 -0.27076334253632695 1105 8 -0.3020947728368455 1105 9 -0.4835506589246208 1105 10-11 -0.44769481274900613 1105 12-13 -0.43959546503635494 1105 14-15 -0.6159201893485644 1105 16-17 -0.6335944060248409 1105 18-19 -0.6383865951483116 1105 20-21 -0.689240486076713 1105 22-23 -0.6446801280717338 1105 24-25 -0.67243031083364 1105 26-27 -0.7193960561376187 1105 28-29 -0.6741341476411407 1105 30-31 -0.7035452615346074 1105 32-33 -0.637815553995722 1105 34-35 -0.6282598359370652 1105 36-37 -0.7112580318750759 1105 38-39 -0.6996388882518758 1105 40-41 -0.7310724815020677 1105 42-43 -0.6644733819035267 1105 44-45 -0.6220591311813237 1105 46-47 -0.8329109021890204 1105 48-49 -0.752445619228169 1105 50-51 -0.7465706081529575 1105 52-53 -0.7280466154240273 1105 54-55 -0.7461293387430956 1105 56-57 -0.702051989584163 1105 58-59 -0.7377580033513667 1105 60-61 -0.8247294345997531 1105 62-63 -0.7810894476720875 1105 64-65 -0.804259511218973 1105 66-67 -0.8787662530209914 1105 68-69 -0.8278515877874781 1105 70-71 -0.8151391167463444 1105 72-73 -0.9157167020716841 1105 74-75 -0.9298064440025584 1105 76-77 -0.8453922399275093 1105 78-79 -0.6899728750768119 1105 80-81 -0.5401771964540245 1105 82-83 -0.6754836989866142 1105 84-85 -0.5682985696473715 1105 86-87 -0.6376974529245238 1105 88-89 -0.6816752086238544 1105 90-91 -0.6884304936659014 1105 92-93 -0.8900321649062484 1105 94-95 -0.8068525909175364 1105 96-97 -0.821951006558276 1105 98-99 -0.8472887222888481 1105 100 -0.8103307624352389 1106 1 -0.3542390829207669 1106 2 -0.3339774313088384 1106 3 -0.3950199203217899 1106 4 -0.22048823750478164 1106 5 -0.271206876283955 1106 6 -0.2622771817090097 1106 7 -0.358080839871981 1106 8 -0.3731748731364206 1106 9 -0.4874663567529751 1106 10-11 -0.5230025742014988 1106 12-13 -0.5272906568351701 1106 14-15 -0.6608936849372924 1106 16-17 -0.7501272613687959 1106 18-19 -0.7206775677574413 1106 20-21 -0.7058982847580424 1106 22-23 -0.6365142868741032 1106 24-25 -0.8269450762098174 1106 26-27 -0.737280027224763 1106 28-29 -0.8350425980245859 1106 30-31 -0.8357246672736522 1106 32-33 -0.8080344296582709 1106 34-35 -0.7762573092609912 1106 36-37 -0.8058840327569357 1106 38-39 -0.8768776328214329 1106 40-41 -0.8132401517950356 1106 42-43 -0.7576459052402242 1106 44-45 -0.7891087253705535 1106 46-47 -0.7942719516408232 1106 48-49 -0.8685527909603863 1106 50-51 -0.855163798813301 1106 52-53 -0.9415498408771796 1106 54-55 -0.9261116698879945 1106 56-57 -0.9924786600658848 1106 58-59 -0.965269353823345 1106 60-61 -0.97465318918767 1106 62-63 -1.034579123301107 1106 64-65 -1.0612423725017663 1106 66-67 -0.8952496565179544 1106 68-69 -0.9543187837734024 1106 70-71 -0.9475639759198629 1106 72-73 -1.0500254287696933 1106 74-75 -1.0587486024515336 1106 76-77 -1.016952814083588 1106 78-79 -0.8738729017168794 1106 80-81 -0.8199223989048043 1106 82-83 -0.7890987418646667 1106 84-85 -0.8742782613439566 1106 86-87 -0.8313757200386256 1106 88-89 -0.8811650948092122 1106 90-91 -0.836327925641509 1106 92-93 -0.7683793346593077 1106 94-95 -0.8488028830080268 1106 96-97 -0.9904037416034122 1106 98-99 -1.0664597122300385 1106 100 -1.1234359849412385 1107 1 -0.3757067101060052 1107 2 -0.3643014416207748 1107 3 -0.3797512093605917 1107 4 -0.23790607800890484 1107 5 -0.2579287264623815 1107 6 -0.30780419711076945 1107 7 -0.3372462498991382 1107 8 -0.42318574021636124 1107 9 -0.4752712057226489 1107 10-11 -0.5184983660671989 1107 12-13 -0.5457055441959682 1107 14-15 -0.6658772412481468 1107 16-17 -0.7242648254419564 1107 18-19 -0.7435347990550909 1107 20-21 -0.7098868695992877 1107 22-23 -0.7594590906745182 1107 24-25 -0.7942976921557374 1107 26-27 -0.8001586915303776 1107 28-29 -0.8154588261737388 1107 30-31 -0.8550759074587262 1107 32-33 -0.8413383425400554 1107 34-35 -0.8072177789661268 1107 36-37 -0.8398166996054712 1107 38-39 -0.8924718042160862 1107 40-41 -0.9318025108618997 1107 42-43 -0.8655221183812358 1107 44-45 -0.8608250280835037 1107 46-47 -0.9885044465678234 1107 48-49 -0.9031573312537589 1107 50-51 -0.8458433900485929 1107 52-53 -0.8382976784342517 1107 54-55 -0.9374498626886307 1107 56-57 -0.9167456223147639 1107 58-59 -0.915015997635436 1107 60-61 -0.935510660523704 1107 62-63 -0.935912683131285 1107 64-65 -0.9524940110640046 1107 66-67 -0.9044315883924483 1107 68-69 -0.8616856704340421 1107 70-71 -0.8636344177362147 1107 72-73 -0.9359685583950963 1107 74-75 -0.9105209256018405 1107 76-77 -0.7812647274500435 1107 78-79 -0.7115876126521137 1107 80-81 -0.6624555624861941 1107 82-83 -0.6749356514137368 1107 84-85 -0.7205064087324686 1107 86-87 -0.7538130078027017 1107 88-89 -0.7483554022393903 1107 90-91 -0.7323844164410538 1107 92-93 -0.7811861066737968 1107 94-95 -0.8134377133548512 1107 96-97 -0.8102195196496886 1107 98-99 -0.7512516025711768 1107 100 -0.8458906750466468 1108 1 -0.44784158087940185 1108 2 -0.39208738675049304 1108 3 -0.4470302885802049 1108 4 -0.28440191865156095 1108 5 -0.3565390148704992 1108 6 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0.8243976121637004 2115 60-61 0.8304657168188427 2115 62-63 0.8600495527449183 2115 64-65 0.9801718656680833 2115 66-67 0.9142279091521388 2115 68-69 0.9355042036662553 2115 70-71 0.9723532461000488 2115 72-73 0.969560821284638 2115 74-75 0.6700414785942428 2115 76-77 0.6859067223720992 2115 78-79 0.5106992846398768 2115 80-81 0.6904716525808325 2115 82-83 0.8113225431340503 2115 84-85 0.8411003536213038 2115 86-87 0.8704314478687145 2115 88-89 0.8942612433308277 2115 90-91 0.9833956488908839 2115 92-93 0.9657502148030339 2115 94-95 0.9626601065471263 2115 96-97 1.0681100701052166 2115 98-99 1.12970193287304 2115 100 1.0976271678616776 2116 1 0.163217038385568 2116 2 0.309951597667542 2116 3 0.3840509349298955 2116 4 0.1955692937847786 2116 5 0.319269594557376 2116 6 0.25761439603877534 2116 7 0.33215529806702193 2116 8 0.39951554055149785 2116 9 0.5037660778995701 2116 10-11 0.5245397596870092 2116 12-13 0.4169623460169518 2116 14-15 0.5444199600442658 2116 16-17 0.7213442281658757 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0.5400946503738631 2116 86-87 0.5775983878098607 2116 88-89 0.6104912590033926 2116 90-91 0.43951357565151383 2116 92-93 0.6692247256236179 2116 94-95 0.745773938036308 2116 96-97 0.8649148003326346 2116 98-99 0.9927264931906912 2116 100 0.5326644173449893 2201 1 0.6873449252040444 2201 2 0.6340799119517371 2201 3 0.6290679908120183 2201 4 0.4381774022394751 2201 5 0.46921031931124446 2201 6 0.5175808186066817 2201 7 0.5553659028265159 2201 8 0.5524259052652454 2201 9 0.6987499933145571 2201 10-11 0.6641993105535278 2201 12-13 0.6760511280341319 2201 14-15 0.8286715708775816 2201 16-17 0.7884930365814355 2201 18-19 0.7924561147117899 2201 20-21 0.7928528298863995 2201 22-23 0.7828355677329952 2201 24-25 0.7675426812695392 2201 26-27 0.7876598975191058 2201 28-29 0.8512413616236287 2201 30-31 0.9623253122090674 2201 32-33 0.8173387796568576 2201 34-35 0.9713431320341073 2201 36-37 0.9590062252469806 2201 38-39 0.9907314938037999 2201 40-41 0.9564913434830373 2201 42-43 0.0299704744182705 2201 44-45 0.7539652406550701 2201 46-47 0.9409401552841317 2201 48-49 1.0203920219026443 2201 50-51 1.024252935936687 2201 52-53 1.0339481923724847 2201 54-55 1.0859483990187755 2201 56-57 1.0195699098595128 2201 58-59 1.0336311737491783 2201 60-61 1.027313985668762 2201 62-63 1.0156249494719773 2201 64-65 1.0697592098171356 2201 66-67 1.0639567195952324 2201 68-69 1.044586265870457 2201 70-71 1.0458284103738507 2201 72-73 1.0489206296009854 2201 74-75 1.095635669363567 2201 76-77 1.1050024476066476 2201 78-79 0.8276320546947815 2201 80-81 0.7415784725075696 2201 82-83 0.7746288159259258 2201 84-85 0.7245796812368859 2201 86-87 0.6656439765536426 2201 88-89 0.6848406086855441 2201 90-91 0.06174279630551638 2201 92-93 0.42628865597140475 2201 94-95 0.6513076954789625 2201 96-97 0.5180477143854283 2201 98-99 0.44386312453285726 2201 100 0.6145819288105692 2202 1 0.6844197145262214 2202 2 0.6355288114842637 2202 3 0.6140220611319407 2202 4 0.3743779612623399 2202 5 0.39088881503155193 2202 6 0.4335411648806229 2202 7 0.4568233304163485 2202 8 0.4987439600327832 2202 9 0.6555592989914558 2202 10-11 0.6207690909473556 2202 12-13 0.6730267134243562 2202 14-15 0.8386905153477429 2202 16-17 0.8156366247006375 2202 18-19 0.7638560127231884 2202 20-21 0.8011200417685913 2202 22-23 0.784799196438783 2202 24-25 0.7101864921624781 2202 26-27 0.7802768189252305 2202 28-29 0.7677777868708517 2202 30-31 0.8744665246551548 2202 32-33 0.525857612740154 2202 34-35 0.8588545752946786 2202 36-37 0.911132931293551 2202 38-39 0.9920411597476075 2202 40-41 1.0253558413544965 2202 42-43 1.0940903702019327 2202 44-45 1.0427848705025795 2202 46-47 1.054618941654546 2202 48-49 1.0439211930799033 2202 50-51 1.1221039090054248 2202 52-53 1.1349288754524736 2202 54-55 1.0686081608597178 2202 56-57 1.0466562816758511 2202 58-59 1.0527897155558108 2202 60-61 1.0394590705490359 2202 62-63 1.0595772950358224 2202 64-65 1.0663773564788386 2202 66-67 1.059916117340343 2202 68-69 1.040225643914006 2202 70-71 1.1115181648702261 2202 72-73 1.0692807137676894 2202 74-75 1.092961079145418 2202 76-77 1.1065520739385057 2202 78-79 0.9373448593117644 2202 80-81 0.8104027231698012 2202 82-83 0.8186231491188671 2202 84-85 0.7842571553307316 2202 86-87 0.7282993090260526 2202 88-89 0.745552762385504 2202 90-91 0.7315505582300013 2202 92-93 0.7092234911710484 2202 94-95 0.7235916832681752 2202 96-97 0.5093223369753801 2202 98-99 0.447374421598159 2202 100 0.721846180238586 2203 1 0.642366802552317 2203 2 0.5900411288633975 2203 3 0.5362742508784848 2203 4 0.3241990832053574 2203 5 0.48123012659182507 2203 6 0.46851352885975217 2203 7 0.46798372758126305 2203 8 0.4842719447142656 2203 9 0.6523312441268345 2203 10-11 0.6354947246063958 2203 12-13 0.6594005415024711 2203 14-15 0.8067447992880261 2203 16-17 0.7726947409009668 2203 18-19 0.7704791707289687 2203 20-21 0.7802432668003974 2203 22-23 0.7460102327062543 2203 24-25 0.7521214978390205 2203 26-27 0.7212311962509332 2203 28-29 0.7751981004424096 2203 30-31 0.9049847742761088 2203 32-33 0.9287265333855856 2203 34-35 0.9767037752544852 2203 36-37 1.0115345362098225 2203 38-39 0.9936931007336369 2203 40-41 0.9982428740609492 2203 42-43 1.018949261624023 2203 44-45 1.0642763900189252 2203 46-47 1.0925268420060164 2203 48-49 1.0606708717934623 2203 50-51 0.9507623805815015 2203 52-53 0.9977614122409904 2203 54-55 1.023565480941194 2203 56-57 1.059114838293553 2203 58-59 1.0906743290611942 2203 60-61 1.0874334187491215 2203 62-63 1.12069483626059 2203 64-65 1.1485013868252167 2203 66-67 1.0987880336106812 2203 68-69 0.9989198528903884 2203 70-71 1.0696097190396614 2203 72-73 1.056882668497181 2203 74-75 1.0452266465351414 2203 76-77 1.0120457995507373 2203 78-79 0.8970360491101843 2203 80-81 0.8329377345592093 2203 82-83 0.8051476917946552 2203 84-85 0.7754979655613887 2203 86-87 0.744884194544337 2203 88-89 0.7916177301491167 2203 90-91 0.8481865714517944 2203 92-93 0.8144117253096219 2203 94-95 0.7988932001611175 2203 96-97 0.7543061122921131 2203 98-99 0.7249254384268298 2203 100 0.8584381749005559 2204 1 0.6550314297031576 2204 2 0.6338883424524866 2204 3 0.6307935344251518 2204 4 0.4432539323122171 2204 5 0.5012100557875812 2204 6 0.49558338996632045 2204 7 0.5207963943894143 2204 8 0.5107957166497528 2204 9 0.6953401702940667 2204 10-11 0.6674208087947235 2204 12-13 0.7227770838268981 2204 14-15 0.8791769009428165 2204 16-17 0.8580081018547219 2204 18-19 0.864183724463949 2204 20-21 0.8316526130620332 2204 22-23 0.8325899724904318 2204 24-25 0.8071606733757903 2204 26-27 0.8427269493101406 2204 28-29 0.9128728287050265 2204 30-31 1.0078107368037124 2204 32-33 0.9972692526300477 2204 34-35 1.0392565002618213 2204 36-37 1.1098758148190413 2204 38-39 1.1195314231074391 2204 40-41 1.0872721858795416 2204 42-43 1.1346117449624344 2204 44-45 1.1565545549244334 2204 46-47 1.0997164568607616 2204 48-49 1.1487603957451924 2204 50-51 1.1889007393892186 2204 52-53 1.1908770536832591 2204 54-55 1.1804846096926909 2204 56-57 1.1562685896577278 2204 58-59 1.0995670123331038 2204 60-61 1.1117221536816828 2204 62-63 1.1612585244344444 2204 64-65 1.1488501373211832 2204 66-67 1.1469392994722227 2204 68-69 1.1723425205961036 2204 70-71 1.1071611088827353 2204 72-73 1.1696753875319317 2204 74-75 1.2046231690683769 2204 76-77 1.1815444125177983 2204 78-79 1.0013926271638809 2204 80-81 0.9010967513638306 2204 82-83 0.9563242161677543 2204 84-85 0.9286253676337388 2204 86-87 0.9300061167111231 2204 88-89 0.9261021847827529 2204 90-91 0.9956876892149644 2204 92-93 0.9516069129022959 2204 94-95 0.9497011213341366 2204 96-97 0.8401854151210664 2204 98-99 0.8329007310375154 2204 100 1.1030843060419286 2205 1 0.6383451750306293 2205 2 0.41671692161973795 2205 3 0.5156379890636558 2205 4 0.33287122238717615 2205 5 0.4378243356512854 2205 6 0.49133863057281957 2205 7 0.5063878010334477 2205 8 0.5169560111395697 2205 9 0.6776096170944044 2205 10-11 0.6743898123312349 2205 12-13 0.686178035786206 2205 14-15 0.8886307067352988 2205 16-17 0.8606793013222571 2205 18-19 0.879189144503691 2205 20-21 0.8480194143258615 2205 22-23 0.8453921122948529 2205 24-25 0.835621364307876 2205 26-27 0.8476491395870269 2205 28-29 0.8800186162169439 2205 30-31 0.9467856528953362 2205 32-33 1.0214475491195572 2205 34-35 1.0109152339342913 2205 36-37 0.9909259452970929 2205 38-39 1.0336683921643157 2205 40-41 1.0749904173048677 2205 42-43 1.1311881408523305 2205 44-45 1.0894071079142904 2205 46-47 1.1215750119000916 2205 48-49 1.142633883897851 2205 50-51 1.1470887925429452 2205 52-53 1.2081067940251273 2205 54-55 1.2124423967992186 2205 56-57 1.1653632326196472 2205 58-59 1.196010529829259 2205 60-61 1.172698637999126 2205 62-63 1.2359261654636455 2205 64-65 1.166225972774825 2205 66-67 1.185223923569204 2205 68-69 1.205036710040865 2205 70-71 1.180419658113827 2205 72-73 1.1641365158603278 2205 74-75 1.2262313176788169 2205 76-77 1.1432357849643218 2205 78-79 1.0851827670180967 2205 80-81 1.0508125326585613 2205 82-83 0.9256104600535444 2205 84-85 0.8853939571254337 2205 86-87 0.9108700277809838 2205 88-89 0.9740972442194611 2205 90-91 0.9935593827388267 2205 92-93 0.9967802326751602 2205 94-95 1.0397908349936458 2205 96-97 0.96718355470502 2205 98-99 1.0584256510704435 2205 100 1.20215753554994 2206 1 0.6572088733497807 2206 2 0.6657894983036954 2206 3 0.6061542593705553 2206 4 0.27253667078279165 2206 5 0.44497209634968726 2206 6 0.4622990692783304 2206 7 0.511969945496304 2206 8 0.5360334322555929 2206 9 0.7115330984332928 2206 10-11 0.7043010734868957 2206 12-13 0.763787800331734 2206 14-15 0.955546036626842 2206 16-17 0.9200482231010412 2206 18-19 0.8649870638527588 2206 20-21 0.873717357261107 2206 22-23 0.8545743504598633 2206 24-25 0.9014831656710882 2206 26-27 0.9155534649669619 2206 28-29 0.9567341257349398 2206 30-31 0.9806722237887797 2206 32-33 1.0396151770422932 2206 34-35 1.0249615459338486 2206 36-37 1.096414647553722 2206 38-39 1.0730195344261446 2206 40-41 1.0505493717855074 2206 42-43 1.1604510048654433 2206 44-45 1.142780889838555 2206 46-47 1.143632869340955 2206 48-49 1.1934522655070552 2206 50-51 1.1813002848828589 2206 52-53 1.2074672539291313 2206 54-55 1.2521855345632744 2206 56-57 1.2203631916567161 2206 58-59 1.31144722873659 2206 60-61 1.2290560763286393 2206 62-63 1.2839149068942675 2206 64-65 1.2966237274748238 2206 66-67 1.3355691855553076 2206 68-69 1.339373659926089 2206 70-71 1.3302996586413087 2206 72-73 1.2920702664956423 2206 74-75 1.292938342686547 2206 76-77 1.219485273639652 2206 78-79 1.2114947289041424 2206 80-81 1.0503713086255289 2206 82-83 1.056000861871535 2206 84-85 1.0790571134371483 2206 86-87 1.1128844224604073 2206 88-89 1.2090372677028292 2206 90-91 1.2434877268226252 2206 92-93 1.258356315851426 2206 94-95 1.2690028100535429 2206 96-97 1.2504587374752525 2206 98-99 1.2655967599073925 2206 100 1.5811852847717134 2207 1 0.7027837299204762 2207 2 0.6394896334796414 2207 3 0.6163205785492494 2207 4 0.3950064208756743 2207 5 0.5125576429638841 2207 6 0.5504276980738467 2207 7 0.5767088444703319 2207 8 0.5766542606752623 2207 9 0.7168581812946542 2207 10-11 0.7359329197990832 2207 12-13 0.7793860668139487 2207 14-15 0.95449415315462 2207 16-17 0.9242577851576428 2207 18-19 0.9492056852488915 2207 20-21 0.9702983107038818 2207 22-23 0.9448270135940291 2207 24-25 0.935785901752169 2207 26-27 0.9270410068611241 2207 28-29 0.9795845486236203 2207 30-31 1.010680607548501 2207 32-33 1.0099361991405473 2207 34-35 1.0157991126387813 2207 36-37 0.9921109885135024 2207 38-39 1.0164935791784302 2207 40-41 1.1036418462063864 2207 42-43 1.1994282318842764 2207 44-45 1.1053890829448036 2207 46-47 1.0857546975473227 2207 48-49 1.1126961905805999 2207 50-51 1.0833696501037835 2207 52-53 1.0849497419150183 2207 54-55 1.1477754538499099 2207 56-57 1.1149473468621665 2207 58-59 1.0746951559277775 2207 60-61 1.056506637812582 2207 62-63 1.1724088029807191 2207 64-65 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1.0806181832439918 2311 44-45 1.0593811008755338 2311 46-47 1.0744723364782516 2311 48-49 1.0401922616787616 2311 50-51 1.035829168306698 2311 52-53 1.075164382225985 2311 54-55 1.0779422946594153 2311 56-57 1.111907271778385 2311 58-59 1.1276679252321173 2311 60-61 1.135555373296171 2311 62-63 1.1390528966313767 2311 64-65 1.1617487012860508 2311 66-67 1.1721648246900074 2311 68-69 1.1742741963117354 2311 70-71 1.2089064357442467 2311 72-73 1.2352935311166107 2311 74-75 1.1145810268936387 2311 76-77 1.0539369040727387 2311 78-79 1.0109932121465803 2311 80-81 0.9679547613928605 2311 82-83 1.0295416842654284 2311 84-85 1.04048344092163 2311 86-87 1.1016396563272046 2311 88-89 1.1446037410980061 2311 90-91 1.1939234288273362 2311 92-93 1.1909349507328635 2311 94-95 1.206192040155944 2311 96-97 1.2779779984160768 2311 98-99 1.2866715259190187 2311 100 1.4609965192107772 2312 1 0.1854940309273232 2312 2 0.19182008119016558 2312 3 0.3164601629306887 2312 4 0.2437465159849026 2312 5 0.3299788512089705 2312 6 0.417735220883813 2312 7 0.4764395181594594 2312 8 0.30585465066821627 2312 9 0.45489467235595527 2312 10-11 0.32089889543129146 2312 12-13 0.4908239706307995 2312 14-15 0.8125312170329764 2312 16-17 0.8615453690465031 2312 18-19 0.9140485120306536 2312 20-21 0.8791676897161906 2312 22-23 0.494786521450969 2312 24-25 0.5144491990270055 2312 26-27 0.7813370155365718 2312 28-29 0.9124084640317278 2312 30-31 0.8620749823826017 2312 32-33 0.7315977937052907 2312 34-35 0.8561678528293655 2312 36-37 0.9340680828102776 2312 38-39 0.9477229690583542 2312 40-41 0.9675006607785122 2312 42-43 0.8476205394170364 2312 44-45 0.8114831092754073 2312 46-47 0.9918548498382407 2312 48-49 1.0768775216199415 2312 50-51 1.0431750678962644 2312 52-53 1.083253623756498 2312 54-55 1.0615237226836385 2312 56-57 1.0496723131224996 2312 58-59 1.1051491486872038 2312 60-61 1.1297601381766427 2312 62-63 1.1217408744116923 2312 64-65 1.1662527800133944 2312 66-67 1.1452522972627222 2312 68-69 1.1412344799749405 2312 70-71 0.9282289670919184 2312 72-73 1.0828045596826072 2312 74-75 1.102411609671016 2312 76-77 1.0619564700396786 2312 78-79 0.9607218641444888 2312 80-81 0.8613803833263631 2312 82-83 0.8539532878411471 2312 84-85 0.8125213224920138 2312 86-87 0.9997624096242497 2312 88-89 0.565697142632299 2312 90-91 0.9324659257183043 2312 92-93 1.1450765618512548 2312 94-95 1.200106909694604 2312 96-97 1.334080059125359 2312 98-99 1.3861410776274248 2312 100 1.4497339389397474 2313 1 -0.32342500633046356 2313 2 -0.05944545033307236 2313 3 0.21157407193751965 2313 4 0.03630657567599371 2313 5 0.18459016660221295 2313 6 0.30362850965733656 2313 7 0.1544248545250042 2313 8 0.11360108863818397 2313 9 0.2669548638499748 2313 10-11 0.3442816288006654 2313 12-13 0.5319202359165871 2313 14-15 0.5919349986601006 2313 16-17 0.7317166905160875 2313 18-19 0.6233409536911054 2313 20-21 0.8077774145384851 2313 22-23 0.8602654352161139 2313 24-25 0.6846268872482923 2313 26-27 0.8739179626411513 2313 28-29 0.7534948999464604 2313 30-31 0.7858247534029346 2313 32-33 0.9002346163164106 2313 34-35 0.9413002589253594 2313 36-37 0.8076366806504964 2313 38-39 0.5644774671125887 2313 40-41 0.7354576930233065 2313 42-43 0.752220349978252 2313 44-45 0.9505452532399445 2313 46-47 0.9936369818288213 2313 48-49 0.9552312360052255 2313 50-51 0.8706986472170613 2313 52-53 0.8409769403650031 2313 54-55 0.9548778391795665 2313 56-57 0.9636094676982907 2313 58-59 0.9174053691153219 2313 60-61 0.9214483996736575 2313 62-63 0.9563644988582283 2313 64-65 1.0263026417320162 2313 66-67 1.010205674768656 2313 68-69 1.0998399826355794 2313 70-71 0.9651114480251763 2313 72-73 0.9903258156975099 2313 74-75 0.6685610429184194 2313 76-77 0.8180942420786899 2313 78-79 0.6493787699478375 2313 80-81 0.7982691193139217 2313 82-83 0.881906678750763 2313 84-85 0.8474407216493738 2313 86-87 0.9884859987744044 2313 88-89 1.037997863303918 2313 90-91 1.0970861098849731 2313 92-93 0.8071690338933983 2313 94-95 0.8707799758775465 2313 96-97 1.146928834251689 2313 98-99 1.2250259578207938 2313 100 1.3225329417053722 2314 1 -0.28502216910301215 2314 2 -0.12713425932229683 2314 3 0.17368312997356128 2314 4 -0.11227125600571952 2314 5 0.20602766009219664 2314 6 0.21177202956167207 2314 7 0.16444145183929493 2314 8 0.2117396660155677 2314 9 0.2719780015628288 2314 10-11 0.4803620155904298 2314 12-13 0.5456320775717103 2314 14-15 0.610850641575361 2314 16-17 0.7849352621393635 2314 18-19 0.858269525935583 2314 20-21 0.5324279000103118 2314 22-23 0.7117151627121174 2314 24-25 0.9266506992123666 2314 26-27 0.9228113146346644 2314 28-29 0.8747135095410243 2314 30-31 0.8584028747722314 2314 32-33 0.9063152062630238 2314 34-35 0.9704877426486931 2314 36-37 0.8037349739295934 2314 38-39 0.8543573967238274 2314 40-41 0.9240398389844415 2314 42-43 0.7956617572756954 2314 44-45 0.8260341923017123 2314 46-47 0.9375061086075078 2314 48-49 0.7831456148102731 2314 50-51 0.8922752470745863 2314 52-53 1.0038436156482362 2314 54-55 1.0606970817993755 2314 56-57 0.9400705787581245 2314 58-59 0.9203404182816186 2314 60-61 1.0219691083973927 2314 62-63 0.6680545100456854 2314 64-65 0.808767001006359 2314 66-67 0.8932121211778323 2314 68-69 1.0145973987600954 2314 70-71 1.01613419799272 2314 72-73 1.0491563082514048 2314 74-75 1.0404412879313085 2314 76-77 0.8446390960063148 2314 78-79 0.8209305372063511 2314 80-81 0.8055699086421484 2314 82-83 0.8204513815234371 2314 84-85 0.7813363469653574 2314 86-87 0.9530160591805803 2314 88-89 1.0311899901130097 2314 90-91 1.1576901629865723 2314 92-93 1.2207763580027304 2314 94-95 1.2221590939828175 2314 96-97 1.3009281371029395 2314 98-99 1.378630884731539 2314 100 1.3521992653264547 2315 1 -0.1010448209383128 2315 2 0.09177460971119444 2315 3 0.11863694310531514 2315 4 -0.07484105871139946 2315 5 0.2593222683706671 2315 6 0.36873490196114744 2315 7 0.41138527790282353 2315 8 0.408269968615393 2315 9 0.5631933316254916 2315 10-11 0.6285934207056485 2315 12-13 0.6603620981654004 2315 14-15 0.7198796878104972 2315 16-17 0.7664982630622745 2315 18-19 0.8528130462556973 2315 20-21 0.8596970779399697 2315 22-23 0.9438904982187637 2315 24-25 0.8530726940109403 2315 26-27 0.9352154337695069 2315 28-29 0.9891193384010819 2315 30-31 0.9708347790422778 2315 32-33 0.9828954648251695 2315 34-35 0.9883646092858456 2315 36-37 1.0219497873934245 2315 38-39 1.044064607997477 2315 40-41 0.9613797231330992 2315 42-43 1.0242836046404449 2315 44-45 0.9580425687791418 2315 46-47 0.996866552534236 2315 48-49 1.0887804219013972 2315 50-51 1.0127066239005913 2315 52-53 1.0589647452430526 2315 54-55 0.9737156320049323 2315 56-57 1.1263603826063644 2315 58-59 1.1020757282852216 2315 60-61 1.1418700243750806 2315 62-63 1.149095088563051 2315 64-65 1.1854980156938382 2315 66-67 1.1604382367303927 2315 68-69 1.1221935973442285 2315 70-71 0.785625111382835 2315 72-73 0.9814958571149077 2315 74-75 1.1911502327591066 2315 76-77 1.044221715617585 2315 78-79 0.9843049746681061 2315 80-81 0.9673778235411277 2315 82-83 1.0071641678485683 2315 84-85 0.9427609942590998 2315 86-87 0.9930180808202955 2315 88-89 1.1019373369304581 2315 90-91 1.1726550512212874 2315 92-93 1.272649059313597 2315 94-95 1.2960903324177693 2315 96-97 1.4111920867224903 2315 98-99 1.3426532779619258 2315 100 1.2757301893112718 2316 1 0.4872820665002493 2316 2 0.5019200908197021 2316 3 0.47485153059085405 2316 4 0.3100048555186703 2316 5 0.3835066878524742 2316 6 0.4120910695726536 2316 7 0.47003786545829485 2316 8 0.4725559489462938 2316 9 0.5565500313273688 2316 10-11 0.4873450910893453 2316 12-13 0.6444258585544631 2316 14-15 0.7651429053153578 2316 16-17 0.800739057518804 2316 18-19 0.822232330426921 2316 20-21 0.8710214812766353 2316 22-23 0.9140445421815926 2316 24-25 0.92115493009792 2316 26-27 0.9873357568851802 2316 28-29 0.9805035410940732 2316 30-31 0.8116430889256279 2316 32-33 0.7718859639715419 2316 34-35 0.6995907849395167 2316 36-37 0.6749504730298739 2316 38-39 0.6406795630814415 2316 40-41 0.7480570544110492 2316 42-43 0.8823095607688316 2316 44-45 0.8027033079590851 2316 46-47 0.8940356797387992 2316 48-49 0.6462682256299743 2316 50-51 0.722044809898108 2316 52-53 0.855652349665796 2316 54-55 0.8557451919053776 2316 56-57 0.9478232548605945 2316 58-59 0.9091306955008491 2316 60-61 0.940140312600839 2316 62-63 0.983270796965364 2316 64-65 0.9337060303430462 2316 66-67 0.9295862878353915 2316 68-69 0.9248500015079131 2316 70-71 0.778675859121833 2316 72-73 0.8478223850124067 2316 74-75 0.9085964308213832 2316 76-77 0.7255551872944075 2316 78-79 0.712460289566792 2316 80-81 0.7550166611212958 2316 82-83 0.6968603518484535 2316 84-85 0.7571987723713356 2316 86-87 0.721635858263614 2316 88-89 0.7267262490792952 2316 90-91 0.8070121454301677 2316 92-93 0.8280260225370313 2316 94-95 0.7895024645342872 2316 96-97 1.026808368991869 2316 98-99 1.0623378281859353 2316 100 0.7275023542957726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 3.0 11 3.0 12 38.0 13 166.0 14 663.0 15 1950.0 16 4546.0 17 9650.0 18 16255.0 19 23250.0 20 31877.0 21 42129.0 22 52055.0 23 57131.0 24 60715.0 25 67021.0 26 79148.0 27 97331.0 28 119886.0 29 146658.0 30 180644.0 31 223272.0 32 281540.0 33 365939.0 34 515069.0 35 682914.0 36 1028513.0 37 1571980.0 38 1345709.0 39 199178.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.73067338042424 19.281594427629347 12.290386585852369 24.697345606094036 2 16.179554936254902 20.581117128320145 39.57969594435177 23.659631991073187 3 18.02352725647652 26.71177722941296 30.895623475072377 24.369072039038148 4 12.329818857956292 16.27077400588422 37.79073197376741 33.60867516239208 5 12.311822367948972 38.39736259502307 34.288638064800075 15.002176972227884 6 30.341139667409646 36.78573708651735 18.858236899154228 14.014886346918779 7 27.292856540121868 33.189336439217634 22.609450778099518 16.90835624256098 8 27.02531843211003 35.71585897733936 20.07754095118473 17.181281639365878 9 28.528253074712584 14.290899072815327 19.900834750723266 37.28001310174882 10-11 25.840723882299248 25.51031688274774 28.800882750794926 19.848076484158085 12-13 25.18657959295157 23.59123975953043 28.419325890227608 22.80285475729039 14-15 23.177984964451305 25.033012352438476 26.130759379522523 25.658243303587692 16-17 22.411863118525083 27.387751898596797 27.121626707061257 23.078758275816867 18-19 22.191940332479327 27.188366554433845 28.571182719291183 22.048510393795645 20-21 23.091773875982383 26.863331829107867 27.884467989593986 22.160426305315763 22-23 22.89497316439098 26.386603617344196 28.015809564639493 22.702613653625335 24-25 22.235913616393365 25.926906691448576 28.5546620411544 23.282517651003655 26-27 22.37494707858891 26.292078723882184 28.090279052406093 23.24269514512281 28-29 22.149536205942617 27.660395979759873 27.93444143712211 22.255626377175403 30-31 23.622321379004052 26.4104258035863 28.30829817354133 21.658954643868316 32-33 23.56777613685837 26.281511673349833 27.836709062906856 22.314003126884945 34-35 23.257865078619204 26.690216774919705 28.212029267952747 21.839888878508347 36-37 22.694683514147982 26.59502801191904 28.218024905823462 22.49226356810951 38-39 22.875727807089337 26.826423770015733 28.106242621982304 22.191605800912626 40-41 22.829502491863916 26.061965381071495 28.639780526668822 22.468751600395766 42-43 22.499346993770278 26.45580140172194 27.872935952094185 23.171915652413595 44-45 21.539011508712054 26.89250478046239 28.727447262580203 22.841036448245358 46-47 21.587264770337896 26.20066166627523 28.30667262982501 23.905400933561864 48-49 22.12681236511748 26.4475221728159 28.25323554962254 23.172429912444077 50-51 21.954048020680347 26.644366780248124 28.19723892816747 23.20434627090406 52-53 21.69480665320952 26.983286744135494 27.7697414611842 23.55216514147079 54-55 21.569894356642347 26.383564880006627 28.473245209988225 23.573295553362797 56-57 22.13853467315453 26.28909398294888 27.808433012386747 23.76393833150984 58-59 21.33245298079813 26.552358710253927 28.455466401432012 23.659721907515934 60-61 22.266956431646562 26.7398892972311 27.80914538969663 23.184008881425708 62-63 22.207863747123426 26.660660348906784 27.482297138271562 23.64917876569823 64-65 21.5220357257156 26.264397058881713 27.531280632455505 24.68228658294718 66-67 21.421587236173174 26.193797037425 27.484136996808378 24.900478729593452 68-69 20.994569313549153 26.435691514235632 27.809461121624395 24.76027805059082 70-71 21.3579212268277 26.124790736001778 27.957621562656797 24.559666474513723 72-73 21.358577671638475 26.77806665255076 27.30555275937117 24.5578029164396 74-75 21.70356417628896 26.770499466136418 27.05600646161659 24.469929895958035 76-77 21.542359625599314 26.450109212810496 27.1181791103975 24.889352051192684 78-79 21.253850868391684 26.432123284975955 27.4583251872489 24.85570065938346 80-81 21.263439916292892 26.842484160228768 26.99267590574096 24.90140001773738 82-83 21.75029691520546 26.689341663849646 27.15813103418117 24.402230386763723 84-85 21.74383551752771 26.9151205125428 26.62214270440199 24.7189012655275 86-87 21.17109761528166 27.096110643399484 26.860757503994527 24.872034237324332 88-89 21.211981708892086 26.56267792974077 26.967698096731564 25.25764226463558 90-91 21.931678817037348 26.143540445327012 26.900191101165188 25.024589636470452 92-93 21.671323871215296 26.509693989141585 26.683432464217542 25.135549675425573 94-95 21.192374098952 26.19588186131191 26.948886603817723 25.66285743591836 96-97 21.02821654850004 26.064261261556975 27.151199453657902 25.75632273628508 98-99 21.030273188740065 26.279401379929567 26.90586408292071 25.78446134840966 100 20.95819878916495 26.431609641038794 27.031932741380636 25.578258828415617 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 314.0 1 297.0 2 563.5 3 921.5 4 837.5 5 793.0 6 1132.5 7 2042.0 8 3222.0 9 4019.5 10 4152.5 11 4213.5 12 4714.0 13 5340.0 14 6113.5 15 6935.5 16 7816.0 17 8594.5 18 9357.5 19 10116.0 20 11119.0 21 12520.0 22 14429.5 23 16966.5 24 20277.5 25 24587.5 26 29790.0 27 35306.5 28 41780.5 29 51839.0 30 65379.0 31 77735.5 32 93226.5 33 109609.5 34 124025.5 35 139404.0 36 155478.0 37 176794.5 38 189507.0 39 190315.5 40 191003.5 41 192677.5 42 198147.0 43 208843.5 44 223993.5 45 248070.0 46 271076.5 47 289432.0 48 311515.0 49 331820.5 50 349241.5 51 359454.5 52 348727.5 53 325144.5 54 300259.5 55 265018.0 56 229658.0 57 196230.5 58 162171.0 59 133634.0 60 103569.0 61 77432.5 62 59701.0 63 46229.5 64 34013.5 65 23388.5 66 16528.0 67 12287.5 68 9211.0 69 6829.0 70 5058.0 71 3745.0 72 2800.5 73 2114.0 74 1625.0 75 1136.0 76 740.5 77 467.5 78 299.5 79 186.0 80 118.5 81 67.5 82 48.0 83 42.5 84 23.5 85 14.0 86 12.0 87 8.5 88 6.5 89 2.5 90 2.5 91 2.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05175385759847667 2 0.0 3 0.0 4 0.017279043365541286 5 0.004149746157668148 6 0.0 7 1.6654499629437385E-4 8 4.857562391919237E-4 9 0.0015960562144877492 10-11 7.702706078614789E-4 12-13 3.330899925887477E-4 14-15 3.469687422799455E-5 16-17 6.245437361039019E-5 18-19 0.0 20-21 2.081812453679673E-5 22-23 2.081812453679673E-5 24-25 5.551499876479128E-5 26-27 6.93937484559891E-6 28-29 9.506943538470506E-4 30-31 8.327249814718692E-4 32-33 0.013399932826851495 34-35 0.02238642325190208 36-37 0.03005443245628888 38-39 0.019818854559030485 40-41 1.52666246603176E-4 42-43 0.001693207462326134 44-45 0.009361216666712928 46-47 0.014211839683786568 48-49 0.005260046132963974 50-51 0.01025639602179519 52-53 0.016071592142407075 54-55 0.010228638522412792 56-57 0.0030394461823723224 58-59 0.007917826698828357 60-61 0.001533601840877359 62-63 9.576337286926495E-4 64-65 0.019451067692213744 66-67 0.045550056486511246 68-69 0.05624363312357916 70-71 0.049012804534465104 72-73 0.04142806782822549 74-75 0.029270283098736204 76-77 0.029443767469876175 78-79 0.012192481603717284 80-81 0.0019013887076941012 82-83 0.028104468124675583 84-85 0.04885319891301632 86-87 0.04897810766023711 88-89 0.06084443864621124 90-91 0.03512017609357609 92-93 0.026432078786886245 94-95 0.007279404213033256 96-97 0.0034766267976450538 98-99 0.021636970768577398 100 0.01812564709670435 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7205260.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.42852024116392 #Duplication Level Percentage of deduplicated Percentage of total 1 72.08772296464846 40.67803534450084 2 16.17579648914676 18.255525192095327 3 5.511622526320607 9.330381098644121 4 2.3760953207433873 5.363181716060125 5 1.2260274566828844 3.4591457577826437 6 0.661541852181386 2.2397896677716647 7 0.4407846369318825 1.741097736497338 8 0.2933183102961203 1.32412145677189 9 0.20939201196989568 1.0634113247203167 >10 0.9445318081786536 8.973353431658483 >50 0.04414879316225728 1.7057114848925243 >100 0.025143361492700463 2.7791550734475905 >500 0.0026130286592547973 1.0128714454417223 >1k 0.0011112682067244087 1.1140530893995948 >5k 6.0068551666797274E-5 0.18778270339073483 >10k+ 9.010282750019591E-5 0.7723834769250314 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17699 0.2456399907845102 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16641 0.23095627361122292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11234 0.1559138740309163 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 2.775749938239564E-5 5 0.0 0.0 0.0 0.0 2.775749938239564E-5 6 0.0 0.0 0.0 0.0 2.775749938239564E-5 7 0.0 0.0 0.0 0.0 2.775749938239564E-5 8 0.0 0.0 0.0 2.775749938239564E-5 2.775749938239564E-5 9 0.0 0.0 0.0 2.775749938239564E-5 2.775749938239564E-5 10-11 0.0 0.0 0.0 5.551499876479127E-5 2.775749938239564E-5 12-13 0.0 0.0 0.0 6.93937484559891E-5 3.469687422799455E-5 14-15 0.0 6.93937484559891E-6 0.0 6.93937484559891E-5 4.1636249073593455E-5 16-17 0.0 1.387874969119782E-5 0.0 1.0409062268398365E-4 4.1636249073593455E-5 18-19 0.0 1.387874969119782E-5 0.0 1.457268717575771E-4 5.551499876479128E-5 20-21 0.0 1.387874969119782E-5 0.0 2.983931183607531E-4 7.6333123301588E-5 22-23 0.0 1.387874969119782E-5 0.0 6.86998109714292E-4 1.0409062268398365E-4 24-25 0.0 1.387874969119782E-5 0.0 0.0014156324685021776 1.2490874722078039E-4 26-27 0.0 1.387874969119782E-5 0.0 0.003261506177431488 1.457268717575771E-4 28-29 0.0 1.387874969119782E-5 0.0 0.014426960304000133 1.52666246603176E-4 30-31 0.0 1.387874969119782E-5 0.0 0.058006234334361285 1.6654499629437385E-4 32-33 0.0 1.387874969119782E-5 0.0 0.15307566971906633 1.9430249567676947E-4 34-35 0.0 1.387874969119782E-5 0.0 0.28925396168909934 2.0818124536796728E-4 36-37 0.0 2.081812453679673E-5 0.0 0.5173928491130091 2.0818124536796728E-4 38-39 0.0 3.469687422799455E-5 0.0 0.920653800140453 2.0818124536796728E-4 40-41 0.0 4.857562391919237E-5 0.0 1.431280758779003 2.0818124536796728E-4 42-43 0.0 6.93937484559891E-5 0.0 1.8716743046052469 2.0818124536796728E-4 44-45 0.0 6.93937484559891E-5 0.0 2.347104754026919 2.0818124536796728E-4 46-47 0.0 6.93937484559891E-5 0.0 2.877758748469868 2.28999369904764E-4 48-49 0.0 6.93937484559891E-5 0.0 3.472414597113775 2.4981749444156077E-4 50-51 0.0 6.93937484559891E-5 0.0 4.123543078251166 2.4981749444156077E-4 52-53 0.0 6.93937484559891E-5 0.0 4.776128828106133 2.4981749444156077E-4 54-55 0.0 6.93937484559891E-5 0.0 5.4583595873015 2.4981749444156077E-4 56-57 0.0 6.93937484559891E-5 0.0 6.151388013756616 2.636962441327586E-4 58-59 0.0 6.93937484559891E-5 0.0 6.96378062693088 2.845143686695553E-4 60-61 0.0 6.93937484559891E-5 0.0 7.762974549148817 2.9145374351515423E-4 62-63 0.0 6.93937484559891E-5 0.0 8.596483679978238 2.9145374351515423E-4 64-65 0.0 6.93937484559891E-5 0.0 9.358822582391198 2.9145374351515423E-4 66-67 0.0 6.93937484559891E-5 0.0 10.191707169484516 2.9145374351515423E-4 68-69 0.0 6.93937484559891E-5 0.0 11.157008907381552 2.9145374351515423E-4 70-71 1.387874969119782E-5 6.93937484559891E-5 0.0 12.120783982812556 3.3308999258874764E-4 72-73 1.387874969119782E-5 6.93937484559891E-5 0.0 12.981134615544754 4.0942311589033564E-4 74-75 1.387874969119782E-5 6.93937484559891E-5 0.0 13.786324157629288 4.1636249073593456E-4 76-77 1.387874969119782E-5 6.93937484559891E-5 0.0 14.644946886024933 4.1636249073593456E-4 78-79 1.387874969119782E-5 6.93937484559891E-5 0.0 15.585135026355745 4.1636249073593456E-4 80-81 1.387874969119782E-5 6.93937484559891E-5 0.0 16.47910693021487 4.1636249073593456E-4 82-83 1.387874969119782E-5 6.93937484559891E-5 0.0 17.37686079336485 4.1636249073593456E-4 84-85 1.387874969119782E-5 6.93937484559891E-5 0.0 18.236933851103224 4.1636249073593456E-4 86-87 1.387874969119782E-5 6.93937484559891E-5 0.0 19.07606387555758 4.233018655815335E-4 88 1.387874969119782E-5 6.93937484559891E-5 0.0 19.741883013243104 4.7187748950072586E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 7270 0.0 52.93841 2 GGTATCA 11260 0.0 49.731663 1 ACCTGGG 8335 0.0 45.554382 3 GTATCAA 18715 0.0 37.809963 1 CCTGGGG 7995 0.0 36.266293 4 TGGGGGG 18005 0.0 34.73427 6 GTACATG 29355 0.0 34.260075 1 TACATGG 29790 0.0 33.41003 2 ACATGGG 29100 0.0 32.942883 3 CTGGGGG 6220 0.0 32.256233 5 ATGGGGG 17685 0.0 32.201324 5 CATGGGG 22150 0.0 31.802729 4 GTACCTG 13105 0.0 29.99362 1 CAACGCA 23075 0.0 29.770208 5 ATCAACG 23275 0.0 29.477291 3 TATCAAC 24650 0.0 28.519115 2 AACGCAG 24730 0.0 28.252705 6 TCAACGC 24695 0.0 28.087545 4 GTACTAG 2985 0.0 28.037243 1 ACGCAGA 27725 0.0 24.624489 7 >>END_MODULE