FastQCFastQC Report
Mon 6 Jun 2016
ERR1211403_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211403_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346936
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269430.36672430520695976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220960.30075122472824045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130260.177298400312729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA125100.051.5879171
TACCTGG63800.044.2570882
GTATCAA223600.041.9664541
ACCTGGG77600.035.3159453
ATCAACG271950.033.965463
CAACGCA273550.033.8011675
TATCAAC280050.033.2554242
TCAACGC282950.032.728084
AACGCAG291650.032.0419126
ACGCAGA320300.028.795257
GTACCTG100350.028.6349981
CGCAGAG326300.028.3076348
GTACATG233350.026.2232251
GTGGTAT51550.025.678071
TACATGG236750.025.522742
GCAGAGT351450.025.4921029
CCTGGGG77250.024.1577264
ACATGGG239500.024.0825823
CATGGGG180400.023.1127224
ATGGGGG133700.022.8531445
Produced by FastQC (version 0.11.5)