##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211403_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7346936 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.331054469509468 31.0 31.0 34.0 28.0 34.0 2 31.384025667298584 31.0 31.0 34.0 28.0 34.0 3 31.44727189674716 31.0 31.0 34.0 28.0 34.0 4 35.07609212874591 37.0 35.0 37.0 32.0 37.0 5 35.12871188751338 37.0 35.0 37.0 32.0 37.0 6 35.19137705840911 37.0 35.0 37.0 32.0 37.0 7 35.126366283849485 37.0 35.0 37.0 32.0 37.0 8 35.1294968405877 37.0 35.0 37.0 32.0 37.0 9 36.69895409460488 39.0 37.0 39.0 32.0 39.0 10-11 36.62842829990625 39.0 37.0 39.0 32.0 39.0 12-13 36.53855654928803 39.0 37.0 39.0 32.0 39.0 14-15 37.72187589493089 40.0 37.0 41.0 32.0 41.0 16-17 37.716526045687615 40.0 37.0 41.0 32.0 41.0 18-19 37.72108161824194 40.0 37.0 41.0 32.0 41.0 20-21 37.70941457772328 40.0 37.0 41.0 32.0 41.0 22-23 37.586021301941386 40.0 37.0 41.0 32.0 41.0 24-25 37.494427064561336 40.0 37.0 41.0 31.5 41.0 26-27 37.359286646841625 40.0 37.0 41.0 31.0 41.0 28-29 37.229173086576495 40.0 36.0 41.0 31.0 41.0 30-31 37.071676886800155 39.5 36.0 41.0 30.5 41.0 32-33 36.87601811149573 39.0 36.0 41.0 30.0 41.0 34-35 36.75502542828738 39.0 35.0 41.0 30.0 41.0 36-37 36.62505648613245 39.0 35.0 41.0 30.0 41.0 38-39 36.476108271529796 39.0 35.0 41.0 30.0 41.0 40-41 36.31769250473939 39.0 35.0 40.0 29.0 41.0 42-43 36.12194872801396 39.0 35.0 40.0 28.5 41.0 44-45 35.98390145225166 39.0 35.0 40.0 28.0 41.0 46-47 35.75683475397091 38.0 35.0 40.0 27.0 41.0 48-49 35.51359396624661 38.0 34.0 40.0 26.0 41.0 50-51 34.84993798775435 37.5 33.5 39.5 25.0 40.5 52-53 35.14817544347739 38.0 34.0 40.0 26.0 40.5 54-55 35.55487144572921 38.0 34.0 40.0 27.0 41.0 56-57 35.482980864403885 38.0 34.0 40.0 26.0 41.0 58-59 35.28545001617 38.0 34.0 40.0 26.0 41.0 60-61 35.06888728852409 38.0 34.0 40.0 26.0 41.0 62-63 34.799327297801426 37.0 34.0 40.0 26.0 41.0 64-65 34.59834167604019 37.0 33.5 40.0 26.0 41.0 66-67 34.40746346504176 36.5 33.5 40.0 25.5 41.0 68-69 34.080391403981196 36.0 33.0 39.0 25.0 41.0 70-71 33.73036957175073 35.5 33.0 39.0 24.5 41.0 72-73 33.36603095494503 35.0 32.5 38.5 24.0 40.0 74-75 32.9998706943956 35.0 32.0 37.5 23.0 40.0 76-77 32.62529917233525 35.0 32.0 37.0 22.5 39.0 78-79 32.242120116467596 35.0 31.5 36.5 21.5 39.0 80-81 31.85756722258095 35.0 31.0 36.0 20.0 38.5 82-83 31.500701108598196 35.0 31.0 35.5 20.0 37.0 84-85 31.17294713061336 34.5 31.0 35.0 19.0 37.0 86-87 30.849148611067253 34.0 30.0 35.0 18.0 36.5 88-89 30.543605184528626 34.0 30.0 35.0 18.0 36.0 90-91 30.253243733169853 34.0 29.0 35.0 16.5 36.0 92-93 29.974695777940628 34.0 29.0 35.0 15.0 35.5 94-95 29.70515974278257 34.0 29.0 35.0 11.5 35.0 96-97 29.42688598349026 34.0 28.5 35.0 10.0 35.0 98-99 29.12778224827329 34.0 27.0 35.0 10.0 35.0 100 27.896113835754115 33.0 25.0 35.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3032612932052743 1101 2 -0.3305615510222175 1101 3 -0.3846544660539237 1101 4 -0.27781775626376515 1101 5 -0.2668857136279357 1101 6 -0.2970147846118891 1101 7 -0.322460181228287 1101 8 -0.29380221718651 1101 9 -0.4461085936766551 1101 10-11 -0.3572110167734621 1101 12-13 -0.46976967278542503 1101 14-15 -0.6814442846217403 1101 16-17 -0.5856173124667166 1101 18-19 -0.586928903750028 1101 20-21 -0.6550122402790279 1101 22-23 -0.6206520819288244 1101 24-25 -0.6104807589507146 1101 26-27 -0.7037048891617417 1101 28-29 -0.6953653525538783 1101 30-31 -0.6704047332911784 1101 32-33 -0.6701616881720582 1101 34-35 -0.6893813261692472 1101 36-37 -0.7035214481249312 1101 38-39 -0.807418058108901 1101 40-41 -0.8971629433643358 1101 42-43 -0.8660784377606277 1101 44-45 -0.9866628401160753 1101 46-47 -0.9224404516988685 1101 48-49 -0.9978415040391369 1101 50-51 -0.8601958604972992 1101 52-53 -0.6826381588115638 1101 54-55 -0.6013925920672065 1101 56-57 -0.7204712361714911 1101 58-59 -0.6103144634170476 1101 60-61 -0.6374272206530733 1101 62-63 -0.6414884977078117 1101 64-65 -0.5692877306740343 1101 66-67 -0.773497556026399 1101 68-69 -0.8190503771678834 1101 70-71 -0.8102326920980474 1101 72-73 -0.824512689260807 1101 74-75 -0.8765614893218086 1101 76-77 -0.8705866460243286 1101 78-79 -0.8849420651107316 1101 80-81 -0.9646150418950903 1101 82-83 -0.9532758789375322 1101 84-85 -1.0153097374329256 1101 86-87 -1.0425180712775024 1101 88-89 -1.127818100026463 1101 90-91 -1.1501929689650154 1101 92-93 -1.1890575440091382 1101 94-95 -1.314921883164434 1101 96-97 -1.4504270370043848 1101 98-99 -1.5329577332742836 1101 100 -1.5780341661661872 1102 1 -0.40506161857788925 1102 2 -0.39165028619598274 1102 3 -0.48800175556250736 1102 4 -0.4116780473348385 1102 5 -0.35902343585436824 1102 6 -0.3697989278534095 1102 7 -0.416041456929527 1102 8 -0.4371328473962066 1102 9 -0.6393577911886865 1102 10-11 -0.5604494743643116 1102 12-13 -0.5990336132499507 1102 14-15 -0.8069738005495779 1102 16-17 -0.8278459897678658 1102 18-19 -0.8175534056448939 1102 20-21 -0.839734688955474 1102 22-23 -0.8254394096660889 1102 24-25 -0.8825080932385205 1102 26-27 -0.8829893748839197 1102 28-29 -0.9899931482394848 1102 30-31 -0.9541673611540915 1102 32-33 -0.9870821437551172 1102 34-35 -1.0331346608880452 1102 36-37 -0.8552021182546738 1102 38-39 -0.9654452953308876 1102 40-41 -1.2149424723975955 1102 42-43 -1.1519253200353674 1102 44-45 -1.1359731623856035 1102 46-47 -1.3772906969691476 1102 48-49 -1.2841999006765334 1102 50-51 -1.1695083410358436 1102 52-53 -0.9569507100899628 1102 54-55 -1.028227963087737 1102 56-57 -0.9570785769622461 1102 58-59 -0.9713870627412362 1102 60-61 -0.9867583990262645 1102 62-63 -0.9868913245746 1102 64-65 -1.105167622747281 1102 66-67 -1.0657341400729266 1102 68-69 -1.063743896954918 1102 70-71 -1.0278143644541302 1102 72-73 -0.9477299714592675 1102 74-75 -1.0739260448290437 1102 76-77 -1.150232815778633 1102 78-79 -1.199625695901517 1102 80-81 -1.2612630807420935 1102 82-83 -1.2705783936765584 1102 84-85 -1.2875660743996065 1102 86-87 -1.2119683617445425 1102 88-89 -1.3937352508944016 1102 90-91 -1.5039032383369957 1102 92-93 -1.524656339195623 1102 94-95 -1.4667069617326156 1102 96-97 -1.5676447483494087 1102 98-99 -1.5580699071063258 1102 100 -1.6092223559420944 1103 1 -0.4129200885556408 1103 2 -0.4677137486863323 1103 3 -0.465104504887627 1103 4 -0.32570714736621653 1103 5 -0.2609521361272513 1103 6 -0.3453510760116032 1103 7 -0.36805715195941247 1103 8 -0.44864676423846817 1103 9 -0.5644570664893322 1103 10-11 -0.566086416245092 1103 12-13 -0.6360856084262281 1103 14-15 -0.7976817126290285 1103 16-17 -0.7390809919306705 1103 18-19 -0.7359726167144416 1103 20-21 -0.7194665588432585 1103 22-23 -0.7947594216439313 1103 24-25 -0.7751353424205547 1103 26-27 -0.8045804068970597 1103 28-29 -0.8614124657257847 1103 30-31 -0.9123521319764052 1103 32-33 -0.884845060511779 1103 34-35 -0.8489743373409979 1103 36-37 -0.8416260728291292 1103 38-39 -0.8607207549127551 1103 40-41 -0.942581886807119 1103 42-43 -0.927967950411869 1103 44-45 -0.9844481250551311 1103 46-47 -1.0696667574860683 1103 48-49 -0.9847569153017517 1103 50-51 -0.960702448580939 1103 52-53 -0.7922276637680952 1103 54-55 -0.8656520408750055 1103 56-57 -0.7362339036848695 1103 58-59 -0.8045456085393639 1103 60-61 -0.835476728917449 1103 62-63 -0.8472539537678614 1103 64-65 -0.7958523713210823 1103 66-67 -0.8238523534195963 1103 68-69 -0.8702430652371618 1103 70-71 -0.904184856203571 1103 72-73 -0.8591111619155569 1103 74-75 -0.8644316271963035 1103 76-77 -0.9544242320703233 1103 78-79 -0.9658876237594782 1103 80-81 -0.9668955979921989 1103 82-83 -0.9263006131064024 1103 84-85 -1.0028439230313637 1103 86-87 -1.0943948693183678 1103 88-89 -1.2322295252593598 1103 90-91 -1.2173483999281416 1103 92-93 -1.1883678999032483 1103 94-95 -1.2386280423653062 1103 96-97 -1.2336253799986565 1103 98-99 -1.3422748905941795 1103 100 -1.421534837148382 1104 1 -0.4620424237781009 1104 2 -0.396931587985339 1104 3 -0.359781582031637 1104 4 -0.2380351561413505 1104 5 -0.25383996547488863 1104 6 -0.2789088754203348 1104 7 -0.2677282055871828 1104 8 -0.27045486088606907 1104 9 -0.39073910029976133 1104 10-11 -0.4523828410807553 1104 12-13 -0.49682572867850894 1104 14-15 -0.6616822238253803 1104 16-17 -0.717391911969024 1104 18-19 -0.6440862234409153 1104 20-21 -0.6600338050878705 1104 22-23 -0.6617163315467849 1104 24-25 -0.6631556618698227 1104 26-27 -0.6341431220280498 1104 28-29 -0.7142398236946619 1104 30-31 -0.8084608011760324 1104 32-33 -0.7363325873813835 1104 34-35 -0.756415151524152 1104 36-37 -0.8983534228635648 1104 38-39 -0.8310611910892263 1104 40-41 -0.8404736864060354 1104 42-43 -0.9289326821034436 1104 44-45 -0.9838674568778885 1104 46-47 -0.9799907433155113 1104 48-49 -0.9319243484601074 1104 50-51 -0.8957265456743286 1104 52-53 -0.7084347685494166 1104 54-55 -0.671793907553301 1104 56-57 -0.6536795088196072 1104 58-59 -0.7455733554777879 1104 60-61 -0.7359814444615083 1104 62-63 -0.7240753178989507 1104 64-65 -0.6857971875538666 1104 66-67 -0.6855588407072233 1104 68-69 -0.7328402092001056 1104 70-71 -0.6634727911823006 1104 72-73 -0.7320401655853885 1104 74-75 -0.6604591348899831 1104 76-77 -0.7667124474802272 1104 78-79 -0.7224728805902174 1104 80-81 -0.7476574138052463 1104 82-83 -0.7800432790438272 1104 84-85 -0.8040931439717625 1104 86-87 -0.7630211623756047 1104 88-89 -0.8357608381557391 1104 90-91 -0.7950340735292691 1104 92-93 -0.875303501882378 1104 94-95 -0.7938851577763621 1104 96-97 -0.8834673876193584 1104 98-99 -1.0441928460025984 1104 100 -1.0254549378855593 1105 1 -0.46443988551389737 1105 2 -0.50511438820104 1105 3 -0.47407570179703384 1105 4 -0.28209169388942 1105 5 -0.3058087321324763 1105 6 -0.36389822549349304 1105 7 -0.3843585095964812 1105 8 -0.33041998356934243 1105 9 -0.5206496151422968 1105 10-11 -0.45783483506713907 1105 12-13 -0.5267621880843549 1105 14-15 -0.6106304450350564 1105 16-17 -0.6281574560702339 1105 18-19 -0.6308874129560067 1105 20-21 -0.7210451322195865 1105 22-23 -0.710635122072226 1105 24-25 -0.6598103894352221 1105 26-27 -0.6904007033343476 1105 28-29 -0.6327280920923855 1105 30-31 -0.6303078838048037 1105 32-33 -0.7449089625956802 1105 34-35 -0.772332192891291 1105 36-37 -0.6794030766158201 1105 38-39 -0.8045208922791929 1105 40-41 -0.7951506167649569 1105 42-43 -0.7810001186390707 1105 44-45 -0.7560889178294801 1105 46-47 -0.9471484928185063 1105 48-49 -0.969773496989454 1105 50-51 -0.9272329560524284 1105 52-53 -0.7393710428341862 1105 54-55 -0.6458402665185972 1105 56-57 -0.6297987060821626 1105 58-59 -0.6406598693787231 1105 60-61 -0.618344387847479 1105 62-63 -0.7267624613266008 1105 64-65 -0.7988354523906835 1105 66-67 -0.7091132596876477 1105 68-69 -0.6520640606181871 1105 70-71 -0.6723032735475343 1105 72-73 -0.6958197718700205 1105 74-75 -0.6668493211029229 1105 76-77 -0.7205501776404333 1105 78-79 -0.6713503323945957 1105 80-81 -0.739906386348764 1105 82-83 -0.7861034343039179 1105 84-85 -0.7668811156810236 1105 86-87 -0.7195400876977196 1105 88-89 -0.7437294662889222 1105 90-91 -0.7996924860280359 1105 92-93 -0.7455121479454974 1105 94-95 -0.839188185098461 1105 96-97 -0.9491293726442258 1105 98-99 -0.8367446393471916 1105 100 -0.8054096735392235 1106 1 -0.49915066931261265 1106 2 -0.44485976893004775 1106 3 -0.5000147168788835 1106 4 -0.42959851095529444 1106 5 -0.48270801190458457 1106 6 -0.40646775207963515 1106 7 -0.4106360604264623 1106 8 -0.4232371632978129 1106 9 -0.5890473280794737 1106 10-11 -0.6692684387561272 1106 12-13 -0.6145973737278112 1106 14-15 -0.7636928650470907 1106 16-17 -0.6928301550869094 1106 18-19 -0.7607635833383029 1106 20-21 -0.8134988489839188 1106 22-23 -0.7631418490475994 1106 24-25 -0.8324055844167546 1106 26-27 -0.8344368271899043 1106 28-29 -0.8175369592090576 1106 30-31 -0.8580390014223553 1106 32-33 -0.8919380932518948 1106 34-35 -0.8317113674655374 1106 36-37 -0.8824784139525335 1106 38-39 -0.9639034876813426 1106 40-41 -0.9641374585025773 1106 42-43 -0.944074139871006 1106 44-45 -1.030416749084658 1106 46-47 -0.9577240518997314 1106 48-49 -0.9953118922137989 1106 50-51 -0.9952363271782261 1106 52-53 -0.8232982807802713 1106 54-55 -0.7995840449422573 1106 56-57 -0.8039034501785594 1106 58-59 -0.7576300851964035 1106 60-61 -0.7841727304424424 1106 62-63 -0.7971013744103317 1106 64-65 -0.8148491485847771 1106 66-67 -0.8524945082584949 1106 68-69 -0.7395958762761268 1106 70-71 -0.7094952456217456 1106 72-73 -0.7295498421745634 1106 74-75 -0.7638038855449167 1106 76-77 -0.771443675255906 1106 78-79 -0.7581643290992872 1106 80-81 -0.7250614403782585 1106 82-83 -0.6500282210918975 1106 84-85 -0.6518047589724105 1106 86-87 -0.7104822598642428 1106 88-89 -0.6808854086891394 1106 90-91 -0.6963983571523045 1106 92-93 -0.6147633726758706 1106 94-95 -0.669976965775259 1106 96-97 -0.7235011284611765 1106 98-99 -0.7598091101150821 1106 100 -0.7975171576926492 1107 1 -0.42115358855878426 1107 2 -0.4265404206553498 1107 3 -0.43726099052585354 1107 4 -0.23905118824917082 1107 5 -0.2567166545989181 1107 6 -0.3540892135721876 1107 7 -0.3091430026239479 1107 8 -0.43586649812672107 1107 9 -0.5727275915306578 1107 10-11 -0.45271106406440964 1107 12-13 -0.5391914834655793 1107 14-15 -0.6393013409273607 1107 16-17 -0.6485566591098575 1107 18-19 -0.6213444126630634 1107 20-21 -0.6000575538740165 1107 22-23 -0.568251608389545 1107 24-25 -0.6536100251222621 1107 26-27 -0.747956496150266 1107 28-29 -0.7819075956191099 1107 30-31 -0.7425416510337257 1107 32-33 -0.6580768149672025 1107 34-35 -0.6984959544209701 1107 36-37 -0.689100285290003 1107 38-39 -0.6427224153257356 1107 40-41 -0.7407329775316072 1107 42-43 -0.7635327850819351 1107 44-45 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40-41 1.3002658358445558 2311 42-43 1.3053578250022042 2311 44-45 1.3572880323979817 2311 46-47 1.342953024127695 2311 48-49 1.3495080300910232 2311 50-51 1.2936272170135723 2311 52-53 1.1610249761114844 2311 54-55 1.1249240307822461 2311 56-57 1.1304184381916684 2311 58-59 1.1248564513150896 2311 60-61 1.130566470146242 2311 62-63 1.0814372735588265 2311 64-65 1.0584292447498882 2311 66-67 1.130493124254528 2311 68-69 1.1200126318222345 2311 70-71 1.0563332520495337 2311 72-73 1.0056012715519458 2311 74-75 1.050996233990169 2311 76-77 1.0896146761335785 2311 78-79 1.099453506526018 2311 80-81 1.113600464008936 2311 82-83 1.1674127831292047 2311 84-85 1.1647304879575557 2311 86-87 1.136585820923795 2311 88-89 1.1838908143546014 2311 90-91 1.281029755332792 2311 92-93 1.2867314344428635 2311 94-95 1.3030023990254733 2311 96-97 1.2861333289108217 2311 98-99 1.3795164482614375 2311 100 1.5312819172920662 2312 1 0.5244118756476048 2312 2 0.5853910105899089 2312 3 0.659409212764416 2312 4 0.4982064690936596 2312 5 0.5378679142221543 2312 6 0.44978292317131263 2312 7 0.5397065717478924 2312 8 0.5882696256661646 2312 9 0.748940939143715 2312 10-11 0.7593769454781167 2312 12-13 0.8400610506815624 2312 14-15 1.0031745150374292 2312 16-17 0.9853012527675773 2312 18-19 0.9342596095677536 2312 20-21 0.9943294854028011 2312 22-23 0.9845067394074789 2312 24-25 0.9932245070084988 2312 26-27 0.9882141959728301 2312 28-29 1.0446176918996528 2312 30-31 0.9774185840185083 2312 32-33 1.038426778455218 2312 34-35 1.1026726988019036 2312 36-37 1.148809613881994 2312 38-39 1.1675276883929442 2312 40-41 1.2018996052241135 2312 42-43 1.2654312135416887 2312 44-45 1.2438267200932387 2312 46-47 1.147949885434734 2312 48-49 1.2643214289040898 2312 50-51 1.2125447090426107 2312 52-53 1.091940820743794 2312 54-55 1.0696588059654388 2312 56-57 1.034567499491736 2312 58-59 1.0570281003734365 2312 60-61 1.0147035213065934 2312 62-63 1.0582593837655736 2312 64-65 0.9780080079770457 2312 66-67 0.9601690529870766 2312 68-69 0.9735053600591641 2312 70-71 1.0764873683161298 2312 72-73 1.0281710508126878 2312 74-75 1.025016510834753 2312 76-77 0.9986504698610048 2312 78-79 0.9794670417383813 2312 80-81 0.876932408213758 2312 82-83 0.9646622971309959 2312 84-85 1.0913720803284832 2312 86-87 1.1238313934007031 2312 88-89 1.1400583088090812 2312 90-91 1.1823582214377097 2312 92-93 1.2668752745635032 2312 94-95 1.258560518897827 2312 96-97 1.2657449977237647 2312 98-99 1.172527668042889 2312 100 1.3456576289776692 2313 1 0.5488242419674414 2313 2 0.5738884380552491 2313 3 0.6165843760669993 2313 4 0.5187971546562835 2313 5 0.515220640725282 2313 6 0.5736186700999468 2313 7 0.5723777103136314 2313 8 0.6221447406073111 2313 9 0.7614881920983692 2313 10-11 0.6956294276418689 2313 12-13 0.8128761896697725 2313 14-15 0.9978497296263384 2313 16-17 0.971300765604127 2313 18-19 1.0037815648848962 2313 20-21 1.0079384219247771 2313 22-23 1.0714214671007625 2313 24-25 1.1320306504633706 2313 26-27 1.1607864945257518 2313 28-29 1.144122998479986 2313 30-31 1.1012908921188682 2313 32-33 1.1242381579481275 2313 34-35 1.1002300853051494 2313 36-37 1.1589565346673893 2313 38-39 1.177744641756675 2313 40-41 1.1480371710313833 2313 42-43 1.1980724524944577 2313 44-45 1.1791364526217691 2313 46-47 1.1402553697438975 2313 48-49 1.1924395411978068 2313 50-51 1.2012723900834104 2313 52-53 1.0360848273080379 2313 54-55 1.0136173762804006 2313 56-57 1.0416420044138235 2313 58-59 0.9862640343090021 2313 60-61 0.9834706149129389 2313 62-63 1.0093328399043457 2313 64-65 0.9739442368863749 2313 66-67 0.9687316982831291 2313 68-69 1.0061158423195167 2313 70-71 0.9619517074050421 2313 72-73 0.9940199273426273 2313 74-75 0.955534401531267 2313 76-77 0.9441333138112071 2313 78-79 1.0072220116098407 2313 80-81 1.0050764896371582 2313 82-83 0.9785092525444092 2313 84-85 1.025924423957477 2313 86-87 1.0613908902575595 2313 88-89 1.081712556214164 2313 90-91 1.102307649219501 2313 92-93 1.141781961769201 2313 94-95 1.2129950857148977 2313 96-97 1.2549699673351995 2313 98-99 1.2886390021848406 2313 100 1.5018630641501325 2314 1 0.43569355848444147 2314 2 0.4656844205890991 2314 3 0.5163427042543098 2314 4 0.4229157482275667 2314 5 0.4548381667598278 2314 6 0.5348835420672344 2314 7 0.5627131006047037 2314 8 0.4669360007542096 2314 9 0.7019474792228451 2314 10-11 0.6283861974250087 2314 12-13 0.3724385713017284 2314 14-15 0.7532621594792772 2314 16-17 0.8085709627236426 2314 18-19 0.8532761011731367 2314 20-21 0.9039225618708073 2314 22-23 0.7831395567271144 2314 24-25 0.7819238672743722 2314 26-27 0.8835084078636584 2314 28-29 0.9540397540537313 2314 30-31 0.8793583081211409 2314 32-33 0.94482202910811 2314 34-35 0.9681571455654634 2314 36-37 0.9859402152406957 2314 38-39 1.0354509726515744 2314 40-41 1.0264661994714928 2314 42-43 1.0989370617016334 2314 44-45 1.1135557768040059 2314 46-47 1.0872714786811244 2314 48-49 1.1273218966983762 2314 50-51 1.0072645612428701 2314 52-53 0.8849715685058825 2314 54-55 0.8231576894768224 2314 56-57 0.8326919078058452 2314 58-59 0.8308989276919547 2314 60-61 0.8513894602304077 2314 62-63 0.8779581887534889 2314 64-65 0.822256934357064 2314 66-67 0.8636238472745461 2314 68-69 0.9256105522529623 2314 70-71 0.9497212254759191 2314 72-73 0.9314938882489017 2314 74-75 0.9617800577880047 2314 76-77 0.9980067183936683 2314 78-79 0.9966590523985275 2314 80-81 1.000388407450739 2314 82-83 0.9001122082710147 2314 84-85 0.89566967980954 2314 86-87 1.1364873352853166 2314 88-89 1.2043729054422059 2314 90-91 1.2689592080159713 2314 92-93 1.345951404360978 2314 94-95 1.3079347313414509 2314 96-97 1.2975790742585112 2314 98-99 1.3240292834204546 2314 100 1.3067625495282336 2315 1 0.3424764518062098 2315 2 0.36159921247632454 2315 3 0.4169484760640465 2315 4 0.32713515578533503 2315 5 0.2845336107132894 2315 6 -0.4261733013932272 2315 7 0.10994091048596744 2315 8 0.1942624111519109 2315 9 0.36541816509284075 2315 10-11 0.4875933446463634 2315 12-13 0.5235896673160596 2315 14-15 0.6311417762130844 2315 16-17 0.5050139231661745 2315 18-19 0.6046796142609097 2315 20-21 0.6582849881587123 2315 22-23 0.572091355640147 2315 24-25 0.6458219525810307 2315 26-27 0.700353036898683 2315 28-29 0.7610801303381365 2315 30-31 0.8493560933784465 2315 32-33 0.8513483885743796 2315 34-35 0.8498482940560947 2315 36-37 0.8865828516145413 2315 38-39 0.9930241446873751 2315 40-41 0.9605287746066509 2315 42-43 0.9817967893815194 2315 44-45 0.9933776265267795 2315 46-47 0.9968787964835713 2315 48-49 1.0214051150331613 2315 50-51 0.9130035084420811 2315 52-53 0.7916696458371746 2315 54-55 0.6136626233157969 2315 56-57 0.608774495378853 2315 58-59 0.6069764529618098 2315 60-61 0.5369982014066679 2315 62-63 0.7251200488380292 2315 64-65 0.7453124965468021 2315 66-67 0.7252362638621932 2315 68-69 0.8120777656101126 2315 70-71 0.7857595081543067 2315 72-73 0.8139337914189397 2315 74-75 0.8999988674800008 2315 76-77 0.8832362613875944 2315 78-79 0.8080768207406948 2315 80-81 0.8010348737228803 2315 82-83 0.9130187145008044 2315 84-85 0.858685933424546 2315 86-87 0.9214337945264113 2315 88-89 1.0452361248322397 2315 90-91 1.0241152265254279 2315 92-93 1.173184375520293 2315 94-95 1.2591846086089866 2315 96-97 1.2761054178505447 2315 98-99 1.378302307163647 2315 100 1.3797040861785774 2316 1 0.0025539960773599546 2316 2 -0.030137084005261272 2316 3 0.09803199876466095 2316 4 0.06900066426280915 2316 5 0.06735592651360633 2316 6 0.15516815357898395 2316 7 0.1771004044540092 2316 8 0.1847693776088306 2316 9 0.26240522486185824 2316 10-11 0.18134790998929873 2316 12-13 0.2661959431269594 2316 14-15 0.4952657313105604 2316 16-17 0.5159003630558985 2316 18-19 0.4441536143633016 2316 20-21 0.4006920433142085 2316 22-23 0.26043769341643497 2316 24-25 0.3543192049693644 2316 26-27 0.3391860729272267 2316 28-29 0.5299519919995817 2316 30-31 0.5575971675247757 2316 32-33 0.5550162659274349 2316 34-35 0.5891513200952048 2316 36-37 0.6268728067070626 2316 38-39 0.6679415135027469 2316 40-41 0.6293742017558799 2316 42-43 0.7284365478758872 2316 44-45 0.5665696562527174 2316 46-47 0.5980347702815365 2316 48-49 0.6896728506569829 2316 50-51 0.46505927096655597 2316 52-53 0.26183522440981477 2316 54-55 0.15855805230557252 2316 56-57 0.316309325045502 2316 58-59 0.3414008921099949 2316 60-61 0.43601460853803786 2316 62-63 0.4376521384167873 2316 64-65 0.41952047364575407 2316 66-67 0.39582393412001693 2316 68-69 0.48127323036570147 2316 70-71 0.40491084671564437 2316 72-73 0.40575810012013136 2316 74-75 0.5181524807707234 2316 76-77 0.5948834936871066 2316 78-79 0.6177988023830068 2316 80-81 0.6726977185931524 2316 82-83 0.7743357897137066 2316 84-85 0.8044596531804231 2316 86-87 0.8359322901472446 2316 88-89 0.8668087561245414 2316 90-91 0.9332589008538754 2316 92-93 0.9724043240704248 2316 94-95 1.043075220630545 2316 96-97 1.0866848820727029 2316 98-99 1.2100936177989219 2316 100 1.0997872560927355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 34.0 1 425.0 2 236.0 3 146.0 4 127.0 5 93.0 6 156.0 7 742.0 8 2674.0 9 4779.0 10 6339.0 11 8537.0 12 11048.0 13 13255.0 14 16023.0 15 19394.0 16 24705.0 17 31879.0 18 39282.0 19 48167.0 20 58241.0 21 69000.0 22 75247.0 23 77973.0 24 77739.0 25 80587.0 26 89250.0 27 102605.0 28 121210.0 29 144341.0 30 175084.0 31 216013.0 32 272401.0 33 353143.0 34 482726.0 35 655822.0 36 950357.0 37 1392133.0 38 1358111.0 39 366471.0 40 441.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34506226183323 19.366802919488972 12.980635638854768 23.307499179823033 2 15.83386472245948 20.77916413012986 40.650068605766705 22.73690254164396 3 18.42095589836977 27.120563915342995 30.436831265411392 24.021648920875847 4 12.574059453954177 17.374120881148045 38.880772566767696 31.171047098130078 5 12.847618138669173 37.5900499785635 34.95530057458534 14.60703130818199 6 29.059410491366144 37.664722156588105 19.321766142645373 13.95410120940038 7 26.086411150964693 33.17122991401214 23.34780900933097 17.394549925692196 8 25.303774285873036 35.20682145273454 21.244766410021473 18.24463785137095 9 26.85923252799577 15.752687579053953 21.10031254608431 36.28776734686597 10-11 24.38587265052433 26.178337851710708 29.29926591630147 20.136523581463493 12-13 25.03827020055192 24.480414453812546 28.746871184724494 21.734444160911046 14-15 22.655985059490984 25.77559095156029 26.79953805096224 24.768885937986486 16-17 21.740922413160536 28.22887557437938 27.555745754190607 22.474456258269477 18-19 21.375160717605258 27.69033392300843 29.56870402373784 21.36580133564847 20-21 22.4456813738834 27.018860054051963 29.19792883637305 21.33752973569159 22-23 22.355976410147843 26.860519059795923 29.01134530706324 21.772159222992993 24-25 21.891763256697544 26.839290289608087 29.36925976943643 21.899686684257947 26-27 21.831222000203525 26.968675623878774 29.106079216769775 22.094023159147923 28-29 21.66892972344239 27.465352360254315 29.16453725140296 21.70118066490033 30-31 22.418459607017144 27.03203404305834 29.114820992075774 21.434685357848743 32-33 22.293131163206606 27.158428454557672 28.78384517789476 21.76459520434096 34-35 22.249474152338472 27.233432386497807 28.999500411535912 21.517593049627806 36-37 22.067380228674214 27.120577439013267 28.926442610483527 21.885599721828992 38-39 22.01988553672461 27.150508941983908 29.04248984171786 21.787115679573624 40-41 22.04802258252505 26.635914849100732 29.424320012046046 21.89174255632817 42-43 21.81570681113004 26.932608130026843 28.904560962947563 22.347124095895555 44-45 21.56851785080142 27.27179825010512 29.135752474406264 22.0239314246872 46-47 21.52382273897361 26.83395709167205 28.854898704405546 22.787321464948793 48-49 21.691114244455807 26.88725201509543 28.88649084381759 22.535142896631168 50-51 21.605868166529486 27.11531393529575 28.736136798990913 22.542681099183856 52-53 21.353045602989535 27.3206703222917 28.57071951377436 22.7555645609444 54-55 21.237947340137513 27.077064922136046 28.84908787489533 22.835899862831113 56-57 21.646117881273106 26.999493783959792 28.472824164649918 22.881564170117187 58-59 21.318460669031587 27.06762489020828 28.711981425624195 22.901933015135935 60-61 21.606503551013514 27.123064021848958 28.441061807192263 22.829370619945266 62-63 21.652856680154667 26.860719883752953 28.443024258388 23.043399177704377 64-65 21.362643296245572 26.678476043126416 28.450202457150848 23.508678203477164 66-67 21.652889928923553 26.65767778268386 28.15684853703258 23.532583751360008 68-69 21.370963184974055 26.86135568060775 28.296180540284855 23.47150059413334 70-71 21.552517185466876 26.647815439986623 28.27172699985131 23.527940374695195 72-73 21.767599356510186 26.980451014787356 27.901566320873954 23.350383307828498 74-75 21.946191744454183 27.036992537503934 27.737694539699287 23.279121178342592 76-77 21.87658492819099 26.96757082491243 27.72940502914583 23.426439217750747 78-79 21.83526591024451 27.068984728863487 27.757343369106046 23.338405991785955 80-81 21.991520788621248 27.14289049717193 27.64784078801134 23.21774792619548 82-83 22.395807382007064 26.884035656203366 27.820141687094193 22.900015274695384 84-85 22.5488606598282 27.09259505799953 27.329904844669834 23.02863943750243 86-87 22.27008854778649 27.213029326957734 27.56981238770553 22.947069737550247 88-89 22.556495946444688 26.724869434146793 27.486482590017026 23.232152029391493 90-91 23.093347636928733 26.58938550243024 27.41084389057341 22.906422970067617 92-93 23.256452223881986 26.69501081114012 27.18275608686078 22.865780878117114 94-95 23.026485028462513 26.514551194234322 27.273551560120147 23.185412217183018 96-97 23.18760580116447 26.409243601148297 27.171175358529293 23.231975239157943 98-99 23.3001888624203 26.474524812878613 26.925396995369965 23.299889329331116 100 23.730928753161606 26.3500064876175 26.71166563486545 23.20739912435544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2733.0 1 1890.0 2 2334.5 3 3348.0 4 2359.5 5 1753.0 6 2303.0 7 3898.0 8 6293.0 9 7619.0 10 6697.0 11 5593.5 12 5782.0 13 6329.5 14 7082.0 15 7787.5 16 8449.5 17 9315.0 18 10304.5 19 11325.0 20 12455.5 21 13961.5 22 15951.0 23 19059.0 24 23221.0 25 28296.5 26 35188.5 27 43581.5 28 53246.0 29 65933.5 30 80968.0 31 95300.5 32 112062.5 33 129607.0 34 146238.0 35 162954.0 36 180564.5 37 201794.0 38 213738.0 39 216863.5 40 220909.5 41 225126.0 42 231434.0 43 241751.0 44 255216.5 45 272167.0 46 288315.0 47 300343.5 48 309734.5 49 314617.5 50 313550.0 51 307848.5 52 294547.5 53 275825.0 54 256187.0 55 230247.5 56 203146.5 57 174775.0 58 146024.5 59 122404.0 60 98195.0 61 75521.0 62 58489.0 63 45064.5 64 33349.5 65 23499.0 66 16711.5 67 12504.0 68 9501.0 69 6980.0 70 5181.5 71 3904.5 72 2971.5 73 2255.0 74 1658.5 75 1156.0 76 810.0 77 572.0 78 397.5 79 275.0 80 193.0 81 143.0 82 119.5 83 98.0 84 77.0 85 78.5 86 61.0 87 36.0 88 30.0 89 30.5 90 27.0 91 14.5 92 10.5 93 11.5 94 9.0 95 5.0 96 4.0 97 5.0 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22051369441628454 2 0.1472450556259099 3 0.023411119955311983 4 0.02252639739886124 5 0.027045287994886574 6 0.05500252077873007 7 0.06130446760390998 8 0.05422668715230404 9 0.0416364046181973 10-11 0.043868627683703795 12-13 0.0422216826170801 14-15 0.02092028568099681 16-17 0.01904195163807062 18-19 0.01858597924359216 20-21 0.02173014709805557 22-23 0.02488792606877207 24-25 0.02218611949253403 26-27 0.019851813055129376 28-29 0.019246118381866944 30-31 0.01766042333838215 32-33 0.024976398324417143 34-35 0.02573181527646355 36-37 0.024690564883102287 38-39 0.02311167539774404 40-41 0.02432987030239545 42-43 0.030448067058158668 44-45 0.040180015179116846 46-47 0.04464446131012983 48-49 0.04071084871298729 50-51 0.02839959406206887 52-53 0.04335140526608643 54-55 0.04177932133885473 56-57 0.04401154440436122 58-59 0.04744835125826603 60-61 0.02422098137237074 62-63 0.025711398602083914 64-65 0.026201398787195098 66-67 0.03142806742838103 68-69 0.03572918016435695 70-71 0.040016681784079786 72-73 0.04396390549747541 74-75 0.02550042630016105 76-77 0.018708479289869955 78-79 0.018327368034783482 80-81 0.0212265357966913 82-83 0.01979056303199048 84-85 0.011460559885100401 86-87 0.013822088555011231 88-89 0.0191780628006015 90-91 0.01991986863639482 92-93 0.020464313286518352 94-95 0.023799036768525003 96-97 0.024363898093028167 98-99 0.02955653894358138 100 0.02960417785046719 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7346936.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.47651007211839 #Duplication Level Percentage of deduplicated Percentage of total 1 77.12543048058315 48.95653164717203 2 14.226161298208991 18.060541418666876 3 4.1729300562120555 7.946491102301703 4 1.7018154269654584 4.321012163626374 5 0.8634332602861725 2.7403865021578624 6 0.4857693277916522 1.8500964976975793 7 0.31097812017647103 1.3817864044313142 8 0.21058321509341407 1.0693670059116935 9 0.14867519687489567 0.8493644368713148 >10 0.7041668633149354 7.540386751512185 >50 0.033702163779036516 1.4675652971284987 >100 0.01471636017568036 1.700121873957337 >500 0.0011181723702858826 0.4613844969343431 >1k 4.160465343306698E-4 0.5450359995255355 >5k 2.600290836592757E-5 0.08549972671433814 >10k+ 7.80087250977827E-5 1.0244286753910032 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26943 0.36672430520695976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22096 0.30075122472824045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13026 0.177298400312729 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.3611116253088362E-5 0.0 2 0.0 0.0 0.0 2.7222232506176724E-5 0.0 3 0.0 0.0 0.0 2.7222232506176724E-5 0.0 4 0.0 0.0 0.0 2.7222232506176724E-5 0.0 5 0.0 0.0 0.0 2.7222232506176724E-5 1.3611116253088362E-5 6 2.7222232506176724E-5 0.0 0.0 2.7222232506176724E-5 2.7222232506176724E-5 7 2.7222232506176724E-5 0.0 0.0 4.0833348759265086E-5 2.7222232506176724E-5 8 2.7222232506176724E-5 0.0 0.0 4.0833348759265086E-5 4.0833348759265086E-5 9 2.7222232506176724E-5 0.0 0.0 4.0833348759265086E-5 4.0833348759265086E-5 10-11 3.4027790632720905E-5 0.0 0.0 6.805558126544181E-5 4.0833348759265086E-5 12-13 4.0833348759265086E-5 0.0 0.0 1.0208337189816272E-4 5.444446501235345E-5 14-15 4.0833348759265086E-5 0.0 0.0 1.2930560440433943E-4 8.166669751853017E-5 16-17 4.0833348759265086E-5 0.0 0.0 1.6333339503706034E-4 9.527781377161853E-5 18-19 4.0833348759265086E-5 0.0 0.0 2.24583418175958E-4 1.088889300247069E-4 20-21 4.0833348759265086E-5 0.0 0.0 6.125002313889763E-4 1.3611116253088362E-4 22-23 4.0833348759265086E-5 0.0 0.0 0.0023819453442904637 1.6333339503706034E-4 24-25 4.0833348759265086E-5 0.0 0.0 0.005723474384423657 1.769445112901487E-4 26-27 4.0833348759265086E-5 0.0 0.0 0.011603476605757829 1.769445112901487E-4 28-29 4.0833348759265086E-5 0.0 0.0 0.030788344964485875 1.769445112901487E-4 30-31 4.0833348759265086E-5 0.0 0.0 0.08885336690016082 1.769445112901487E-4 32-33 4.0833348759265086E-5 0.0 0.0 0.22487605717539938 1.8375006941669287E-4 34-35 4.0833348759265086E-5 0.0 0.0 0.42605516095417195 2.109723019228696E-4 36-37 4.0833348759265086E-5 0.0 0.0 0.7332036103213639 2.177778600494138E-4 38-39 4.0833348759265086E-5 0.0 0.0 1.158020159696505 2.450000925555905E-4 40-41 4.0833348759265086E-5 0.0 0.0 1.61055574732106 2.586112088086789E-4 42-43 4.0833348759265086E-5 0.0 0.0 2.05006549669141 2.654167669352231E-4 44-45 4.0833348759265086E-5 0.0 0.0 2.5738893056915155 2.7902788318831144E-4 46-47 4.0833348759265086E-5 0.0 0.0 3.176412588866978 2.8583344131485563E-4 48-49 4.0833348759265086E-5 0.0 0.0 3.7952487948717666 2.8583344131485563E-4 50-51 5.444446501235345E-5 0.0 0.0 4.4158476404313305 2.9944455756794396E-4 52-53 6.805558126544181E-5 0.0 0.0 5.014192310917096 2.9944455756794396E-4 54-55 6.805558126544181E-5 0.0 0.0 5.660277971660568 2.9944455756794396E-4 56-57 6.805558126544181E-5 0.0 0.0 6.3786386597079385 3.266667900741207E-4 58-59 6.805558126544181E-5 0.0 0.0 7.1544178416689626 3.334723482006649E-4 60-61 7.486113939198599E-5 0.0 0.0 7.917457018817096 3.470834644537532E-4 62-63 9.527781377161853E-5 0.0 0.0 8.691922728059698 3.675001388333858E-4 64-65 9.527781377161853E-5 0.0 0.0 9.441405233419754 3.675001388333858E-4 66-67 9.527781377161853E-5 0.0 0.0 10.253226651218958 3.8111125508647414E-4 68-69 9.527781377161853E-5 0.0 0.0 11.13237545556406 3.947223713395625E-4 70-71 1.0208337189816272E-4 0.0 0.0 12.028204682877323 3.947223713395625E-4 72-73 1.088889300247069E-4 0.0 0.0 12.883772228313953 4.0833348759265086E-4 74-75 1.088889300247069E-4 0.0 0.0 13.71330851391655 4.0833348759265086E-4 76-77 1.088889300247069E-4 1.3611116253088362E-5 0.0 14.588530511222636 4.0833348759265086E-4 78-79 1.088889300247069E-4 1.3611116253088362E-5 0.0 15.500577383551455 4.0833348759265086E-4 80-81 1.088889300247069E-4 1.3611116253088362E-5 0.0 16.430529679311213 4.1513904571919506E-4 82-83 1.088889300247069E-4 1.3611116253088362E-5 0.0 17.349872109951686 4.2875016197228345E-4 84-85 1.1569448815125108E-4 2.0416674379632543E-5 0.0 18.23092647057222 4.355557200988276E-4 86-87 1.2250004627779526E-4 2.7222232506176724E-5 0.0 19.112613748098525 4.49166836351916E-4 88 1.2250004627779526E-4 2.7222232506176724E-5 0.0 19.779891916848058 4.627779526050043E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12510 0.0 51.587917 1 TACCTGG 6380 0.0 44.257088 2 GTATCAA 22360 0.0 41.966454 1 ACCTGGG 7760 0.0 35.315945 3 ATCAACG 27195 0.0 33.96546 3 CAACGCA 27355 0.0 33.801167 5 TATCAAC 28005 0.0 33.255424 2 TCAACGC 28295 0.0 32.72808 4 AACGCAG 29165 0.0 32.041912 6 ACGCAGA 32030 0.0 28.79525 7 GTACCTG 10035 0.0 28.634998 1 CGCAGAG 32630 0.0 28.307634 8 GTACATG 23335 0.0 26.223225 1 GTGGTAT 5155 0.0 25.67807 1 TACATGG 23675 0.0 25.52274 2 GCAGAGT 35145 0.0 25.492102 9 CCTGGGG 7725 0.0 24.157726 4 ACATGGG 23950 0.0 24.082582 3 CATGGGG 18040 0.0 23.112722 4 ATGGGGG 13370 0.0 22.853144 5 >>END_MODULE