FastQCFastQC Report
Mon 6 Jun 2016
ERR1211403_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211403_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7346936
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241370.3285315130007938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207020.2817773286714353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129800.17667228896508694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124650.050.27791
TACCTGG68000.042.775912
GTATCAA214200.040.671971
ACCTGGG77650.036.3113253
ATCAACG270350.031.7921283
CAACGCA272550.031.6872315
TATCAAC278000.031.2711932
TCAACGC283000.030.5040824
AACGCAG292450.029.9646536
CCTGGGG73050.027.8549844
GTACCTG109000.027.2171171
ACGCAGA322850.026.7501817
CGCAGAG328100.026.2791828
GTACATG227300.023.786891
GTACTAG26850.023.2887741
GCAGAGT360200.023.2194779
TACATGG229150.022.9881082
GTGGTAT53850.022.874671
ACATGGG228700.022.0683783
TGGGGGG124450.021.9740526
Produced by FastQC (version 0.11.5)