FastQCFastQC Report
Mon 6 Jun 2016
ERR1211402_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211402_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7558091
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295320.39073358603382785No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT232270.3073130503456495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142060.18795751466871727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123450.054.6513331
TACCTGG74850.045.9620322
GTATCAA232000.043.0929531
ACCTGGG91650.036.46813
ATCAACG285700.034.3390433
CAACGCA288200.034.073795
TATCAAC290800.033.9535182
TCAACGC294800.033.342844
AACGCAG305400.032.5248346
TAACGCA8300.030.5838154
GTACCTG115550.030.2947771
ACGCAGA337850.029.0675077
CGCAGAG343200.028.4477528
ATAACGC9600.026.9319293
GTACATG218100.025.4471111
CCTGGGG91200.025.411374
GCAGAGT371400.025.4014479
GTGGTAT52600.025.3483431
TACATGG218250.024.647092
ACATGGG215950.023.7273543
Produced by FastQC (version 0.11.5)