FastQCFastQC Report
Mon 6 Jun 2016
ERR1211402_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211402_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7558091
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265570.35137179480903313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221850.2935265002763264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143260.18954521717190226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126150.055.1605641
TACCTGG74750.045.4530332
GTATCAA227000.040.9067961
ACCTGGG89050.037.0465353
ATCAACG278500.032.6850243
CAACGCA281650.032.2987255
TATCAAC285850.032.1727642
TCAACGC287250.031.7225364
AACGCAG298700.030.8316156
GTACCTG115350.030.2848511
ACGCAGA333400.027.4255627
CCTGGGG82600.027.081774
TAACGCA8050.026.8541934
CGCAGAG338050.026.8537228
ATAACGC8100.025.5277213
GTGGTAT51400.024.0572971
GCAGAGT372400.023.934989
GTACATG207650.023.4348721
TACATGG210400.022.6255662
CTGGGGG57150.022.5280765
Produced by FastQC (version 0.11.5)