FastQCFastQC Report
Mon 6 Jun 2016
ERR1211401_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211401_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7087612
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239790.33832269599408094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195150.2753395642989486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113820.16059005487320696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111900.055.45831
GTATCAA204350.041.2521481
TACCTGG57850.036.7756732
ATCAACG243100.033.6668473
CAACGCA244900.033.3611075
TATCAAC251450.033.0196532
TCAACGC253900.032.3822174
AACGCAG261650.031.7120936
ACGCAGA289350.028.1238337
CGCAGAG292400.027.6516698
ACCTGGG75400.027.4951363
GTACATG210050.025.6858331
TACATGG210000.024.8563752
GTGGTAT49150.024.5429821
GCAGAGT319250.024.3823289
GTACCTG89450.024.3373071
ACATGGG209400.023.6620643
GTACTAG23250.022.2936211
CATGGGG148500.021.8108314
CAGAGTA312550.021.55109410-11
Produced by FastQC (version 0.11.5)