FastQCFastQC Report
Mon 6 Jun 2016
ERR1211401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211401_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7087612
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216570.3055613089429839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186820.26358666360404603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114340.16132372934635814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109900.054.545651
GTATCAA201450.040.2595981
TACCTGG57050.038.5490882
ATCAACG242100.032.803633
CAACGCA246650.032.0987055
TATCAAC253150.031.7611312
TCAACGC256000.031.0231534
AACGCAG264000.030.3798836
ACCTGGG78350.027.9496863
ACGCAGA292300.027.1492187
CGCAGAG294800.026.8711688
GTACTAG23650.025.4464051
GTGGTAT46750.024.4383721
GTACATG207150.024.1719861
GTACCTG95300.023.6807791
GCAGAGT329100.023.6565349
TACATGG207300.023.532
TATCACG4600.023.4959982
ACATGGG202600.023.0324333
CATGGGG147050.021.7631264
Produced by FastQC (version 0.11.5)