##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211401_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7087612 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03966865003333 33.0 31.0 34.0 30.0 34.0 2 32.18057943916794 33.0 31.0 34.0 30.0 34.0 3 32.25915907924983 34.0 31.0 34.0 30.0 34.0 4 35.908640032778315 37.0 35.0 37.0 35.0 37.0 5 35.76397000851627 37.0 35.0 37.0 35.0 37.0 6 35.79798823637637 37.0 35.0 37.0 35.0 37.0 7 35.72870030131446 37.0 35.0 37.0 33.0 37.0 8 35.7122994317409 37.0 35.0 37.0 33.0 37.0 9 37.301210619317196 39.0 37.0 39.0 34.0 39.0 10-11 37.276260960673355 39.0 37.0 39.0 34.0 39.0 12-13 37.20045693810553 39.0 37.0 39.0 33.0 39.0 14-15 38.406840272859185 40.0 38.0 41.0 33.5 41.0 16-17 38.39642068442798 40.0 38.0 41.0 33.5 41.0 18-19 38.40524876361742 40.0 38.0 41.0 33.5 41.0 20-21 38.366484790645984 40.0 38.0 41.0 34.0 41.0 22-23 38.26600108753132 40.0 38.0 41.0 34.0 41.0 24-25 38.14609017818695 40.0 37.5 41.0 33.0 41.0 26-27 38.02467467180766 40.0 37.0 41.0 33.0 41.0 28-29 37.94987204435006 40.0 37.0 41.0 33.0 41.0 30-31 37.99089813324996 40.0 37.0 41.0 33.0 41.0 32-33 37.833744214553505 40.0 37.0 41.0 33.0 41.0 34-35 37.72409557690234 40.0 37.0 41.0 33.0 41.0 36-37 37.57330621371486 40.0 37.0 41.0 32.5 41.0 38-39 37.45390527867496 40.0 36.0 41.0 32.0 41.0 40-41 37.38728241613677 40.0 36.0 41.0 32.0 41.0 42-43 37.24949729189464 40.0 36.0 41.0 31.0 41.0 44-45 37.09445226403477 39.5 36.0 41.0 31.0 41.0 46-47 36.87457587971802 39.0 36.0 41.0 30.5 41.0 48-49 36.6519128022245 39.0 35.0 41.0 30.0 41.0 50-51 36.472618774842644 39.0 35.0 41.0 30.0 41.0 52-53 36.269966104239344 39.0 35.0 40.0 29.0 41.0 54-55 36.07473793712184 39.0 35.0 40.0 29.0 41.0 56-57 35.84836430098036 38.0 35.0 40.0 28.0 41.0 58-59 35.58952747413374 38.0 34.0 40.0 28.0 41.0 60-61 35.34085824675505 38.0 34.0 40.0 27.5 41.0 62-63 35.05703408990222 37.0 34.0 40.0 27.0 41.0 64-65 34.71173273875601 37.0 34.0 39.5 26.0 41.0 66-67 34.36356039241426 36.0 33.0 39.0 26.0 41.0 68-69 33.995462646657295 36.0 33.0 39.0 26.0 40.0 70-71 33.62022864682773 35.0 32.5 38.5 25.5 40.0 72-73 33.26068279414844 35.0 32.0 37.5 25.0 40.0 74-75 32.88872577957146 35.0 32.0 37.0 24.5 39.0 76-77 31.86072389402806 34.0 30.5 36.0 23.5 39.0 78-79 32.33860276211509 35.0 31.5 36.0 24.0 38.5 80-81 32.214146809955174 35.0 31.5 36.0 24.0 37.5 82-83 31.937189493442926 34.5 31.0 35.0 24.0 37.0 84-85 31.65804857827996 34.0 31.0 35.0 23.5 37.0 86-87 31.397039369536593 34.0 31.0 35.0 23.0 36.0 88-89 31.15277543409543 34.0 31.0 35.0 21.5 36.0 90-91 30.92795366055591 34.0 30.5 35.0 20.0 35.5 92-93 30.743697595184386 34.0 30.0 35.0 20.0 35.0 94-95 30.57301739993668 34.0 30.0 35.0 20.0 35.0 96-97 30.398616346380138 34.0 29.5 35.0 19.5 35.0 98-99 30.198471431562563 34.0 29.0 35.0 19.0 35.0 100 28.350196370794563 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2861444345886355 1101 2 -0.2913590354916522 1101 3 -0.32235161915057375 1101 4 -0.13408181776277672 1101 5 -0.1387376285587223 1101 6 -0.21545173433118947 1101 7 -0.23626565456689974 1101 8 -0.2725764222872442 1101 9 -0.4455726079237152 1101 10-11 -0.4010356807499278 1101 12-13 -0.3855730806482214 1101 14-15 -0.575745070708507 1101 16-17 -0.6227504762271607 1101 18-19 -0.5923731542989259 1101 20-21 -0.5665675952227289 1101 22-23 -0.7068665054460936 1101 24-25 -0.7371284570065058 1101 26-27 -0.69107904802636 1101 28-29 -0.6936908706959031 1101 30-31 -0.6462795149867944 1101 32-33 -0.5687379536462132 1101 34-35 -0.6470699931295982 1101 36-37 -0.6615694103706389 1101 38-39 -0.6033130248968703 1101 40-41 -0.6727096411742224 1101 42-43 -0.6522930671300102 1101 44-45 -0.6845458851634447 1101 46-47 -0.7407696035512288 1101 48-49 -0.6739909260871926 1101 50-51 -0.7170611198223682 1101 52-53 -0.694227629949431 1101 54-55 -0.706482681074597 1101 56-57 -0.7806713254723618 1101 58-59 -0.7402238033004735 1101 60-61 -0.6660635168561413 1101 62-63 -0.820697310922597 1101 64-65 -0.9182247619031969 1101 66-67 -0.8749336898428055 1101 68-69 -0.8640367552390984 1101 70-71 -0.881849509800297 1101 72-73 -0.8909211123526788 1101 74-75 -0.8481232084516677 1101 76-77 -0.7465181182397735 1101 78-79 -0.6743641295245055 1101 80-81 -0.6104165689487999 1101 82-83 -0.7275723280230615 1101 84-85 -0.8084560547943767 1101 86-87 -0.8208365640078377 1101 88-89 -0.7924776112919467 1101 90-91 -0.9219118268764532 1101 92-93 -0.966669766096647 1101 94-95 -1.0968146791414064 1101 96-97 -0.9895454624007058 1101 98-99 -1.1346060892699512 1101 100 -1.0437887576148803 1102 1 -0.31005207290153436 1102 2 -0.2544260982397262 1102 3 -0.304731451350289 1102 4 -0.11425473801550368 1102 5 -0.16422153137357753 1102 6 -0.2467763144586641 1102 7 -0.24794915746335988 1102 8 -0.2909314877054072 1102 9 -0.4317624345516151 1102 10-11 -0.3767136031459657 1102 12-13 -0.39373294658854263 1102 14-15 -0.559018465700845 1102 16-17 -0.577080921229502 1102 18-19 -0.6139223449176754 1102 20-21 -0.6736764678614477 1102 22-23 -0.6260685020068735 1102 24-25 -0.705857052493684 1102 26-27 -0.7864982326360845 1102 28-29 -0.7683388575698231 1102 30-31 -0.6452054856076046 1102 32-33 -0.6108792446420139 1102 34-35 -0.6150374986151164 1102 36-37 -0.6399149426581872 1102 38-39 -0.7674170321970237 1102 40-41 -0.7783423918003933 1102 42-43 -0.7983639546035164 1102 44-45 -0.7602142182433127 1102 46-47 -0.8482199568851883 1102 48-49 -0.8752997567897722 1102 50-51 -0.8523180939016086 1102 52-53 -0.8743500563854312 1102 54-55 -1.034687744752901 1102 56-57 -0.9637156722588642 1102 58-59 -1.0214334155063582 1102 60-61 -1.0354182436973218 1102 62-63 -0.8789267926542834 1102 64-65 -0.9816906665489427 1102 66-67 -0.9339745452872421 1102 68-69 -0.9445257381252148 1102 70-71 -0.9523858589979639 1102 72-73 -1.032986406483353 1102 74-75 -0.9836741533817488 1102 76-77 -0.8376042515928326 1102 78-79 -0.9067418960788132 1102 80-81 -0.7004192417759185 1102 82-83 -0.740564316954412 1102 84-85 -0.7740866425127528 1102 86-87 -0.8720503821038434 1102 88-89 -0.7787503942394487 1102 90-91 -0.8562117319606379 1102 92-93 -0.9648007347200362 1102 94-95 -1.0428915369203295 1102 96-97 -1.1146798768983537 1102 98-99 -1.090011862057171 1102 100 -1.055393718771068 1103 1 -0.34883082138785326 1103 2 -0.33952067362782046 1103 3 -0.3432215894422228 1103 4 -0.1940737718534038 1103 5 -0.23184859028546612 1103 6 -0.33844544825218037 1103 7 -0.3920886191538955 1103 8 -0.3675757367493304 1103 9 -0.5516142594528262 1103 10-11 -0.45285718161518673 1103 12-13 -0.5820494088136456 1103 14-15 -0.6977682709493038 1103 16-17 -0.833857041902796 1103 18-19 -0.7348879818917808 1103 20-21 -0.7293287083296818 1103 22-23 -0.7557199168272106 1103 24-25 -0.7103387569338224 1103 26-27 -0.8488138292883392 1103 28-29 -0.9064605259797958 1103 30-31 -0.7730276802800518 1103 32-33 -0.7661907632662732 1103 34-35 -0.7676308094392397 1103 36-37 -0.7838491783798673 1103 38-39 -0.7487721290415479 1103 40-41 -0.7772310574227106 1103 42-43 -0.7655384971235151 1103 44-45 -0.7630473511769011 1103 46-47 -0.9420172429939768 1103 48-49 -0.9252456303046301 1103 50-51 -0.8906784289461243 1103 52-53 -0.8776261213751653 1103 54-55 -0.9290715588600875 1103 56-57 -1.0724651679715649 1103 58-59 -1.1621748062669823 1103 60-61 -1.0766263077662472 1103 62-63 -1.1177978194351397 1103 64-65 -1.004647628348799 1103 66-67 -1.1495509978730567 1103 68-69 -1.120971210253451 1103 70-71 -1.0449739358964152 1103 72-73 -1.1347173548976457 1103 74-75 -1.1662331447216516 1103 76-77 -1.150362451507565 1103 78-79 -0.9474568165997468 1103 80-81 -0.8176247237811154 1103 82-83 -0.7394103450821454 1103 84-85 -0.8541644839465548 1103 86-87 -0.9219345833186239 1103 88-89 -0.9049220798711737 1103 90-91 -1.0258119780298252 1103 92-93 -1.0299793258175143 1103 94-95 -1.106922297541761 1103 96-97 -1.2837318007946408 1103 98-99 -1.2358086435146376 1103 100 -1.2874332348863504 1104 1 -0.4181102576078892 1104 2 -0.3222513364642978 1104 3 -0.3526309153562508 1104 4 -0.22741670306235306 1104 5 -0.2770677488589399 1104 6 -0.3824680000854954 1104 7 -0.3937674537887972 1104 8 -0.38222734203370834 1104 9 -0.5140470258906262 1104 10-11 -0.4474362491806332 1104 12-13 -0.551849198341948 1104 14-15 -0.7331881315588333 1104 16-17 -0.7346592511134205 1104 18-19 -0.7442162440362807 1104 20-21 -0.7110096618730282 1104 22-23 -0.7825872113051062 1104 24-25 -0.8642779916442151 1104 26-27 -0.9002504599447647 1104 28-29 -0.8970632791042803 1104 30-31 -0.8922412431426565 1104 32-33 -0.8112789547188726 1104 34-35 -0.8808598097565365 1104 36-37 -0.7956386700785245 1104 38-39 -1.0082971569727235 1104 40-41 -0.9814726835680005 1104 42-43 -0.7952947708360725 1104 44-45 -0.908390745287285 1104 46-47 -0.9107612311923248 1104 48-49 -0.9373817145497014 1104 50-51 -0.9044340146553154 1104 52-53 -0.7946619748090669 1104 54-55 -0.8013281291217922 1104 56-57 -0.832443434105052 1104 58-59 -0.9303233340457595 1104 60-61 -0.898448434109298 1104 62-63 -0.9763920635013648 1104 64-65 -0.9738029457376172 1104 66-67 -0.9675606645583485 1104 68-69 -0.972014059586364 1104 70-71 -0.9175039242393908 1104 72-73 -0.9211093388602265 1104 74-75 -0.9154112215865382 1104 76-77 -0.7979172082982835 1104 78-79 -0.8315812347577172 1104 80-81 -1.0059386379885211 1104 82-83 -0.952817183857956 1104 84-85 -0.8941090356746457 1104 86-87 -0.7384657406791071 1104 88-89 -0.9080329632972699 1104 90-91 -0.8635659360214767 1104 92-93 -0.9530183913653545 1104 94-95 -0.9947616094039553 1104 96-97 -1.073776357625107 1104 98-99 -1.008020921634369 1104 100 -0.9665592838895734 1105 1 -0.33316348228248316 1105 2 -0.35450939200929454 1105 3 -0.4096469963181981 1105 4 -0.25392141369867005 1105 5 -0.28642505732103274 1105 6 -0.3042621671098402 1105 7 -0.3369106343281203 1105 8 -0.31034019521555223 1105 9 -0.5731181642395526 1105 10-11 -0.5244204361265687 1105 12-13 -0.509153813310121 1105 14-15 -0.7535229190726511 1105 16-17 -0.7047341262674252 1105 18-19 -0.7272618467780276 1105 20-21 -0.8102171087850891 1105 22-23 -0.7419704298892 1105 24-25 -0.6724750689453742 1105 26-27 -0.7041834913177993 1105 28-29 -0.7116948269721917 1105 30-31 -0.713730514312708 1105 32-33 -0.6179757478893535 1105 34-35 -0.6523087788674147 1105 36-37 -0.7674754798796855 1105 38-39 -0.7514239988394067 1105 40-41 -0.7102587827643703 1105 42-43 -0.7678779219333265 1105 44-45 -0.8183058861614043 1105 46-47 -0.8838825387681837 1105 48-49 -0.8164037163685052 1105 50-51 -0.7940031511112764 1105 52-53 -0.7557179490157466 1105 54-55 -0.850732335368221 1105 56-57 -0.8425796916822605 1105 58-59 -0.8603175448252998 1105 60-61 -0.9213614873413931 1105 62-63 -0.8860059702379033 1105 64-65 -0.9589998533946869 1105 66-67 -0.910873466012653 1105 68-69 -0.8927809528468558 1105 70-71 -0.916524136915605 1105 72-73 -1.0305260922802262 1105 74-75 -1.0190285110077006 1105 76-77 -0.8498301539964146 1105 78-79 -0.7481338860723667 1105 80-81 -0.795832044951716 1105 82-83 -0.8401976797934516 1105 84-85 -0.7213881762187491 1105 86-87 -0.7631566268979846 1105 88-89 -0.8673994028757015 1105 90-91 -0.8251608546711076 1105 92-93 -0.8819147128696443 1105 94-95 -0.8006107846833821 1105 96-97 -0.891294311624673 1105 98-99 -0.902511080886601 1105 100 -0.9652455155566564 1106 1 -0.42403834806481555 1106 2 -0.3522992188101455 1106 3 -0.39964741870092624 1106 4 -0.20247008441576497 1106 5 -0.28292105913505594 1106 6 -0.35538181479817865 1106 7 -0.3510015444295149 1106 8 -0.37546438685281913 1106 9 -0.4172257028820141 1106 10-11 -0.48808246102936437 1106 12-13 -0.5375289291874878 1106 14-15 -0.6650666945962413 1106 16-17 -0.6983531459393433 1106 18-19 -0.7036097478693364 1106 20-21 -0.6826355166689737 1106 22-23 -0.6800563469821483 1106 24-25 -0.776812883981755 1106 26-27 -0.7452253650075846 1106 28-29 -0.7437533981181161 1106 30-31 -0.7821692988285989 1106 32-33 -0.7398346653891181 1106 34-35 -0.8132702219935766 1106 36-37 -0.8561835222691272 1106 38-39 -0.9079889935373942 1106 40-41 -0.8472884095781126 1106 42-43 -0.8121845362245423 1106 44-45 -0.907428085930654 1106 46-47 -0.8881616648360904 1106 48-49 -0.9410396891492709 1106 50-51 -0.9419399782166877 1106 52-53 -0.9872088044296916 1106 54-55 -0.9468914313802586 1106 56-57 -0.9142124121160293 1106 58-59 -0.9587836841804744 1106 60-61 -1.006759883176258 1106 62-63 -1.020578107650124 1106 64-65 -1.1328839849202197 1106 66-67 -1.0506608630012835 1106 68-69 -1.0425483088865874 1106 70-71 -0.9232403001687501 1106 72-73 -0.9852985169126995 1106 74-75 -1.0385130818112742 1106 76-77 -1.0238198256271893 1106 78-79 -0.866836048449624 1106 80-81 -0.8166481217275603 1106 82-83 -0.9025828861718743 1106 84-85 -0.7160439231803721 1106 86-87 -0.7139345336136458 1106 88-89 -0.8421073474501952 1106 90-91 -0.7856591217903919 1106 92-93 -0.833398054317172 1106 94-95 -0.8531275743968081 1106 96-97 -0.9024867314108604 1106 98-99 -0.9344170384838222 1106 100 -0.9773023193643233 1107 1 -0.4062669796666363 1107 2 -0.3104695089141991 1107 3 -0.434081930570386 1107 4 -0.19785256054365874 1107 5 -0.24292921375075593 1107 6 -0.3561651858395223 1107 7 -0.31982471208976193 1107 8 -0.3866837462333663 1107 9 -0.5430309602748835 1107 10-11 -0.5007501264073895 1107 12-13 -0.5933077440718151 1107 14-15 -0.7283697568093288 1107 16-17 -0.6734773358498316 1107 18-19 -0.7280363014621543 1107 20-21 -0.7312754868952425 1107 22-23 -0.7739585029377523 1107 24-25 -0.821612680312704 1107 26-27 -0.7912088786347127 1107 28-29 -0.7920748082165261 1107 30-31 -0.8681820277081371 1107 32-33 -0.8754620461365832 1107 34-35 -0.9255503686021385 1107 36-37 -0.8736087776244403 1107 38-39 -0.9252631005382526 1107 40-41 -0.9279676096578697 1107 42-43 -0.8161918382924043 1107 44-45 -0.8615198574260177 1107 46-47 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1.0113663147436114 2115 54-55 0.9931336030120406 2115 56-57 0.8948491580922493 2115 58-59 0.8742901072902356 2115 60-61 0.9085706827769755 2115 62-63 0.9183343759535489 2115 64-65 0.998963681202568 2115 66-67 0.9960658702104723 2115 68-69 1.057532473018263 2115 70-71 1.045997390588525 2115 72-73 1.01223683516843 2115 74-75 0.6448430464159998 2115 76-77 0.6600757817567562 2115 78-79 0.46858641691813574 2115 80-81 0.6468376574579011 2115 82-83 0.7811643544462221 2115 84-85 0.8443513482788205 2115 86-87 0.8173060684914866 2115 88-89 0.8892564270643142 2115 90-91 0.9866646776785295 2115 92-93 0.9501804241036602 2115 94-95 0.9596023740947324 2115 96-97 0.9966234679044916 2115 98-99 1.055496420901278 2115 100 1.0369648847819484 2116 1 0.21967116614024818 2116 2 0.33324515992902803 2116 3 0.3643214459073576 2116 4 0.23928133105111016 2116 5 0.34024715576643416 2116 6 0.2896471634803959 2116 7 0.38564838806421875 2116 8 0.38220408205108924 2116 9 0.49784621360575443 2116 10-11 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0.6691571093913105 2116 78-79 0.6741156453109269 2116 80-81 0.602396736892544 2116 82-83 0.6326001313130369 2116 84-85 0.5570213244795355 2116 86-87 0.5665791155343918 2116 88-89 0.5447803696384845 2116 90-91 0.4484741284310054 2116 92-93 0.6475935328173676 2116 94-95 0.7052726042477175 2116 96-97 0.8101278025670027 2116 98-99 0.7864204402435853 2116 100 0.5060527767334086 2201 1 0.6702368318985243 2201 2 0.6204482900841128 2201 3 0.5903551870904238 2201 4 0.38320668382078793 2201 5 0.48562286888128625 2201 6 0.4993955019980305 2201 7 0.5313772323935524 2201 8 0.5274134871797003 2201 9 0.6681667793959463 2201 10-11 0.5716840085655832 2201 12-13 0.6301391338586697 2201 14-15 0.8344464898403317 2201 16-17 0.7935285125083098 2201 18-19 0.7942423915245556 2201 20-21 0.7374687653655414 2201 22-23 0.7607572029089624 2201 24-25 0.6995900659643794 2201 26-27 0.775295675707973 2201 28-29 0.8053018695688721 2201 30-31 0.9259803066221153 2201 32-33 0.7626015396046597 2201 34-35 0.9007244449507468 2201 36-37 0.9589448178529736 2201 38-39 0.9802965329010362 2201 40-41 0.9106107348210699 2201 42-43 -0.0628467738855889 2201 44-45 0.7092156156253466 2201 46-47 0.9377207286070544 2201 48-49 1.0185441231486578 2201 50-51 1.0092438935302326 2201 52-53 0.948856943240699 2201 54-55 1.0258565253406573 2201 56-57 1.018811333052689 2201 58-59 1.0409934101018692 2201 60-61 1.0508228157126638 2201 62-63 1.0575664961366442 2201 64-65 1.0267274246953448 2201 66-67 0.9459170976017219 2201 68-69 0.965354600055413 2201 70-71 1.0373611142357575 2201 72-73 1.006832441036103 2201 74-75 1.0195236573277384 2201 76-77 1.0683971710177538 2201 78-79 0.8728261950891465 2201 80-81 0.8320615077490672 2201 82-83 0.8120363590107011 2201 84-85 0.7333620207668474 2201 86-87 0.7800207861611703 2201 88-89 0.7289037415774651 2201 90-91 0.14329855327274643 2201 92-93 0.5639678572857605 2201 94-95 0.7864609176896771 2201 96-97 0.7887538631797852 2201 98-99 0.8362971974497349 2201 100 1.0065379117590112 2202 1 0.6674842465261683 2202 2 0.63669329903977 2202 3 0.6124831510079787 2202 4 0.4169072794025652 2202 5 0.4053165669410532 2202 6 0.45310157187972777 2202 7 0.514951264749925 2202 8 0.5429950608395941 2202 9 0.686617471029642 2202 10-11 0.6593730654544387 2202 12-13 0.6929409064389063 2202 14-15 0.8396422332126292 2202 16-17 0.8258153533602908 2202 18-19 0.8186912045434127 2202 20-21 0.795951401600469 2202 22-23 0.7906003824322099 2202 24-25 0.8014972267273635 2202 26-27 0.8591532020194919 2202 28-29 0.8581984429303589 2202 30-31 0.934092241195124 2202 32-33 0.523512257943537 2202 34-35 0.8915231390761491 2202 36-37 0.991842867645623 2202 38-39 1.0507239764964496 2202 40-41 1.0456235085869992 2202 42-43 1.0508143607181992 2202 44-45 1.0480675178367136 2202 46-47 1.0404683046594343 2202 48-49 1.0533412564754272 2202 50-51 1.0686408864522363 2202 52-53 1.0648083437291191 2202 54-55 1.094569750880133 2202 56-57 1.054450607367606 2202 58-59 1.0957168033624924 2202 60-61 1.1005497838061729 2202 62-63 1.0505991312570586 2202 64-65 1.0513135026686413 2202 66-67 1.0516345532602571 2202 68-69 1.065823798370083 2202 70-71 1.0938733620230963 2202 72-73 1.1143241067500398 2202 74-75 1.0533575391696246 2202 76-77 1.046901028553652 2202 78-79 0.9620961837738307 2202 80-81 0.8994579053049918 2202 82-83 0.9019819347864875 2202 84-85 0.9127035852038361 2202 86-87 0.8914317773761731 2202 88-89 0.9243462191248035 2202 90-91 0.9921204642779813 2202 92-93 0.9423643140427487 2202 94-95 0.9918855078901494 2202 96-97 0.8065819850227456 2202 98-99 0.8107946775700441 2202 100 0.9322914968382356 2203 1 0.6348983860781452 2203 2 0.6029420147103508 2203 3 0.5734310267387883 2203 4 0.331623430334659 2203 5 0.4556632293093301 2203 6 0.42620530483637253 2203 7 0.5009224381802042 2203 8 0.5042242141642745 2203 9 0.6583550330020032 2203 10-11 0.6644990580297403 2203 12-13 0.6792822933039417 2203 14-15 0.8240535023979305 2203 16-17 0.7857874363379054 2203 18-19 0.7681473502537415 2203 20-21 0.7736620545489572 2203 22-23 0.7745945934385006 2203 24-25 0.8079835909976936 2203 26-27 0.8135445428843369 2203 28-29 0.8517117006203705 2203 30-31 0.9391613267364889 2203 32-33 0.9866494739533138 2203 34-35 1.0503504214423387 2203 36-37 1.0534861352660059 2203 38-39 1.0718004114505462 2203 40-41 1.0528983670674492 2203 42-43 1.0872714116324218 2203 44-45 1.0631499570258143 2203 46-47 1.0738208295380645 2203 48-49 1.1354939880371901 2203 50-51 1.1390727316123375 2203 52-53 1.0953964897888468 2203 54-55 1.1176593757184037 2203 56-57 1.1268625760067223 2203 58-59 1.1792816497923226 2203 60-61 1.1949144752880017 2203 62-63 1.1499606544448042 2203 64-65 1.1862915096081679 2203 66-67 1.1678450064911985 2203 68-69 1.1154021393679656 2203 70-71 1.1660043989344047 2203 72-73 1.1725572796819463 2203 74-75 1.167850107794692 2203 76-77 1.1123902625471693 2203 78-79 0.9992850068623724 2203 80-81 0.9355104376961592 2203 82-83 0.8661645671940725 2203 84-85 0.8502084816217774 2203 86-87 0.9073265755343662 2203 88-89 0.9328908726064142 2203 90-91 1.0371458886516294 2203 92-93 0.971091394986189 2203 94-95 0.9573444102334996 2203 96-97 0.9916323262316915 2203 98-99 0.9110839503005614 2203 100 1.1908884484872857 2204 1 0.7137914495698787 2204 2 0.6453773101932896 2204 3 0.6352186656784795 2204 4 0.4417269485140878 2204 5 0.5073748498081869 2204 6 0.5290550169842732 2204 7 0.5441861749188632 2204 8 0.5870276243371961 2204 9 0.7174850857821866 2204 10-11 0.7320508937215138 2204 12-13 0.7319395522001955 2204 14-15 0.907547678668152 2204 16-17 0.8519381040399665 2204 18-19 0.8842236794663236 2204 20-21 0.8612753201199794 2204 22-23 0.8847475941425316 2204 24-25 0.9141766261362321 2204 26-27 0.8810873868578355 2204 28-29 0.9246527599631804 2204 30-31 0.9956929654408171 2204 32-33 1.0321437102859505 2204 34-35 1.022415342514627 2204 36-37 1.0362279798416907 2204 38-39 1.0047952016441641 2204 40-41 0.9626897354606712 2204 42-43 1.053154538417182 2204 44-45 1.0568138757613426 2204 46-47 1.0341587211458503 2204 48-49 1.0223512209513075 2204 50-51 1.0185414190803925 2204 52-53 1.0549380809216018 2204 54-55 1.026808939163658 2204 56-57 1.0396992171961656 2204 58-59 1.0862840053497962 2204 60-61 1.018641624171508 2204 62-63 1.1206660132746862 2204 64-65 1.0931949402222116 2204 66-67 1.0788418827634558 2204 68-69 1.1000489368130886 2204 70-71 1.051056414059481 2204 72-73 1.1144374997915136 2204 74-75 1.1029146696153447 2204 76-77 1.0570889090794005 2204 78-79 0.9568403105820877 2204 80-81 0.9057160703523337 2204 82-83 0.8937104354650351 2204 84-85 0.9298171126317811 2204 86-87 0.9717341493172249 2204 88-89 0.9319077112312044 2204 90-91 1.0320472715602556 2204 92-93 1.0548886186323436 2204 94-95 1.057990934830908 2204 96-97 1.059028543434259 2204 98-99 1.0695211546908077 2204 100 1.1652918098787381 2205 1 0.6538524346049606 2205 2 0.42246003412279265 2205 3 0.5589756868893616 2205 4 0.37233929128594667 2205 5 0.48429590614166784 2205 6 0.4990689818840224 2205 7 0.5327400653802812 2205 8 0.5567720078566651 2205 9 0.7225488635628992 2205 10-11 0.7212111509243684 2205 12-13 0.7261368196288487 2205 14-15 0.9131186965374241 2205 16-17 0.8883751589278219 2205 18-19 0.8962188011634069 2205 20-21 0.88711511798315 2205 22-23 0.9038566680624598 2205 24-25 0.9438154250470632 2205 26-27 0.9130564392840412 2205 28-29 0.9085881263549282 2205 30-31 0.9716664878221835 2205 32-33 1.0168500244373817 2205 34-35 1.0674145391755516 2205 36-37 1.0500606039689089 2205 38-39 1.0303664491504705 2205 40-41 1.0468018598820734 2205 42-43 1.096445680762237 2205 44-45 1.1094688665043932 2205 46-47 1.1210740498318117 2205 48-49 1.0887746233707603 2205 50-51 1.1471110779514575 2205 52-53 1.1256301702304086 2205 54-55 1.0769359542188681 2205 56-57 1.087964086799424 2205 58-59 1.0850537991443332 2205 60-61 1.0772786674525463 2205 62-63 1.115995141334274 2205 64-65 1.0431410965864458 2205 66-67 1.1028394761048546 2205 68-69 1.2023865246541376 2205 70-71 1.2108438685821739 2205 72-73 1.1602546651285266 2205 74-75 1.1065123477193595 2205 76-77 1.06745103808964 2205 78-79 1.0418221110660895 2205 80-81 1.0148688557597225 2205 82-83 1.0050793119767825 2205 84-85 0.9627174682020794 2205 86-87 0.9922807354463821 2205 88-89 0.9869110374533427 2205 90-91 1.0051705609498605 2205 92-93 1.0714854399715286 2205 94-95 1.1632347199271997 2205 96-97 1.1427021001752635 2205 98-99 1.1782816747421414 2205 100 1.3498076502647542 2206 1 0.6749518355808597 2206 2 0.6252723593490828 2206 3 0.601690438314975 2206 4 0.2646826570997973 2206 5 0.46222450085024747 2206 6 0.45853385057087337 2206 7 0.5014413086217147 2206 8 0.5228971596351073 2206 9 0.7031423804745742 2206 10-11 0.70142484252613 2206 12-13 0.7035830291931688 2206 14-15 0.9157760346799577 2206 16-17 0.9041816634848701 2206 18-19 0.867421462040781 2206 20-21 0.855676814510268 2206 22-23 0.8476183309863572 2206 24-25 0.9264315136786792 2206 26-27 0.9229611834834017 2206 28-29 0.9523401828021179 2206 30-31 0.9511313409519957 2206 32-33 1.0365849945795205 2206 34-35 1.026914022292651 2206 36-37 1.0339180671901076 2206 38-39 1.0395990511210798 2206 40-41 1.0155846998366158 2206 42-43 1.0709084764725318 2206 44-45 1.119624079165341 2206 46-47 1.1533264535900898 2206 48-49 1.1046279546401863 2206 50-51 1.0486028821100106 2206 52-53 1.0628566287230399 2206 54-55 1.0860050516223225 2206 56-57 1.0674741331516486 2206 58-59 1.1108742583728102 2206 60-61 1.093902967093726 2206 62-63 1.0641229673691441 2206 64-65 1.0919113119971016 2206 66-67 1.1049163808124973 2206 68-69 1.171558913073163 2206 70-71 1.2363657102876644 2206 72-73 1.205376130664618 2206 74-75 1.2002993686303682 2206 76-77 1.1423218562580963 2206 78-79 1.0463511953549514 2206 80-81 1.0383803769200313 2206 82-83 0.9456877169827287 2206 84-85 1.002087921168851 2206 86-87 1.0435487903720961 2206 88-89 1.04720816927831 2206 90-91 1.067213582408474 2206 92-93 1.1826508883917484 2206 94-95 1.1796204522479776 2206 96-97 1.2659115423695582 2206 98-99 1.2324112540562489 2206 100 1.3965541660695457 2207 1 0.7064122395774355 2207 2 0.6617199732824233 2207 3 0.6519843122905371 2207 4 0.4307930096947601 2207 5 0.5095589422098925 2207 6 0.5433321774316653 2207 7 0.5692452934270733 2207 8 0.5951531841246336 2207 9 0.711443568899675 2207 10-11 0.7303956156289146 2207 12-13 0.7607699547491364 2207 14-15 0.9795606466019677 2207 16-17 0.9272876864139761 2207 18-19 0.9264009565846152 2207 20-21 0.9537228304505376 2207 22-23 0.9479389319586815 2207 24-25 1.0071148976961979 2207 26-27 1.0185832675926534 2207 28-29 1.0364659225219057 2207 30-31 1.0596594282813996 2207 32-33 1.1373198985921604 2207 34-35 1.108069621686873 2207 36-37 1.1483095825639325 2207 38-39 1.2218511338205715 2207 40-41 1.2139899659697022 2207 42-43 1.2610458687631976 2207 44-45 1.238526153816757 2207 46-47 1.2666338229694745 2207 48-49 1.2967943233921844 2207 50-51 1.2626859493392075 2207 52-53 1.2346681871767586 2207 54-55 1.3027159891211184 2207 56-57 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2311 40-41 1.1308676822860022 2311 42-43 1.1761250844702076 2311 44-45 1.1158762880330642 2311 46-47 1.2120093117992567 2311 48-49 1.2074691403341191 2311 50-51 1.260029027285377 2311 52-53 1.312823812568631 2311 54-55 1.250876068406967 2311 56-57 1.2627043684811952 2311 58-59 1.3362037484442766 2311 60-61 1.3540965136877787 2311 62-63 1.3555970739115537 2311 64-65 1.3570053381437432 2311 66-67 1.431301775317948 2311 68-69 1.407670044544851 2311 70-71 1.4613045216740304 2311 72-73 1.448156530884269 2311 74-75 1.3652647261325157 2311 76-77 1.3146727573050931 2311 78-79 1.2129792278333227 2311 80-81 1.2239555162932518 2311 82-83 1.2087692836799953 2311 84-85 1.3044771784823155 2311 86-87 1.3217809448892943 2311 88-89 1.2839619568031004 2311 90-91 1.3546508285757461 2311 92-93 1.3939864341050026 2311 94-95 1.4154914256646016 2311 96-97 1.4635915364568461 2311 98-99 1.5160621970663932 2311 100 1.5449334236945305 2312 1 0.21373833860113578 2312 2 0.17236935597159686 2312 3 0.3212142402946938 2312 4 0.2553008273412303 2312 5 0.3057603854437829 2312 6 0.37873126430609716 2312 7 0.4498955623943317 2312 8 0.27406064684523557 2312 9 0.3655089161004028 2312 10-11 0.2741743840340902 2312 12-13 0.458026120730878 2312 14-15 0.7851927105881344 2312 16-17 0.8718186509488604 2312 18-19 0.904038812994088 2312 20-21 0.881773479947249 2312 22-23 0.50612764906856 2312 24-25 0.4984307200404601 2312 26-27 0.7386748807587011 2312 28-29 0.9405027896169926 2312 30-31 0.873193050095658 2312 32-33 0.7670651532468113 2312 34-35 0.8838040015539477 2312 36-37 0.9696007808896425 2312 38-39 0.9560234770724492 2312 40-41 0.9813490179527662 2312 42-43 0.8609216861733202 2312 44-45 0.7499586746248568 2312 46-47 0.9010199613729455 2312 48-49 0.9563957988637881 2312 50-51 0.9596270978830361 2312 52-53 0.9741591836798449 2312 54-55 0.9947878429505437 2312 56-57 1.018362864620535 2312 58-59 1.0758283789922416 2312 60-61 1.0368336271034835 2312 62-63 1.0197206440913362 2312 64-65 1.1097014444788584 2312 66-67 1.1709174701790275 2312 68-69 1.192195619142609 2312 70-71 0.9949692917687329 2312 72-73 1.0770005089373385 2312 74-75 1.0959263994049735 2312 76-77 0.9966538233568123 2312 78-79 0.9615287341862668 2312 80-81 0.8548726619568612 2312 82-83 0.8016209548078841 2312 84-85 0.6623120108047047 2312 86-87 0.8705744727161324 2312 88-89 0.5075181483736415 2312 90-91 0.8469954601474505 2312 92-93 1.056963037167229 2312 94-95 1.1285796696543038 2312 96-97 1.2139753517877487 2312 98-99 1.2106092879156272 2312 100 1.277998421660282 2313 1 -0.3844889628191268 2313 2 -0.012394203069810317 2313 3 0.24789217371520778 2313 4 0.08864594848375162 2313 5 0.2330447219225249 2313 6 0.3157165121761025 2313 7 0.11874641898936744 2313 8 0.07615019262450318 2313 9 0.26102623150943316 2313 10-11 0.3450456326848226 2313 12-13 0.5434793805014522 2313 14-15 0.6046317902506644 2313 16-17 0.7239489771380434 2313 18-19 0.6052890610648305 2313 20-21 0.807274938759825 2313 22-23 0.8327883563428102 2313 24-25 0.7239537471270765 2313 26-27 0.9119705173046739 2313 28-29 0.7232875556104759 2313 30-31 0.7504122973671628 2313 32-33 0.8685179936105456 2313 34-35 0.8654970142588709 2313 36-37 0.771340849446851 2313 38-39 0.5763544013078743 2313 40-41 0.703821486727179 2313 42-43 0.736915421265941 2313 44-45 0.9049485695681412 2313 46-47 0.9544441562214487 2313 48-49 1.0366218913217935 2313 50-51 0.9284559164000399 2313 52-53 0.8423178766338921 2313 54-55 0.9430684244141219 2313 56-57 1.0178430549794157 2313 58-59 0.9321933759755296 2313 60-61 0.9517562532337536 2313 62-63 0.9288510639565857 2313 64-65 1.0166271382868146 2313 66-67 1.071680545749416 2313 68-69 1.072225354887827 2313 70-71 0.9758802516693947 2313 72-73 1.087680534551616 2313 74-75 0.744748145236457 2313 76-77 0.9258094674253421 2313 78-79 0.7921994391741336 2313 80-81 0.8828144179361104 2313 82-83 0.8675228019348076 2313 84-85 0.9161623605808913 2313 86-87 0.9715884307350215 2313 88-89 0.8949596542178604 2313 90-91 1.0194818877949317 2313 92-93 0.7955371692470692 2313 94-95 0.7602190748767619 2313 96-97 1.037960019948109 2313 98-99 1.1118797589126395 2313 100 1.191138711110792 2314 1 -0.3615543234337011 2314 2 -0.22855126311030816 2314 3 0.08554298234053448 2314 4 -0.16103612106372367 2314 5 0.16946632395755046 2314 6 0.20686110528477286 2314 7 0.1561765910714854 2314 8 0.18501702683989407 2314 9 0.29878804683295357 2314 10-11 0.4531585561633378 2314 12-13 0.5287208799937986 2314 14-15 0.5952982306748993 2314 16-17 0.7483476657717816 2314 18-19 0.8572888706465847 2314 20-21 0.44516302322703183 2314 22-23 0.6478601662046231 2314 24-25 0.8756894071453445 2314 26-27 0.8675898983361421 2314 28-29 0.8164574133620306 2314 30-31 0.8395790938222163 2314 32-33 0.9111593459858511 2314 34-35 0.9211994357032651 2314 36-37 0.7908129260279892 2314 38-39 0.8814528050612083 2314 40-41 0.9282764702612383 2314 42-43 0.721124657130261 2314 44-45 0.7998166168636587 2314 46-47 0.8874967183445577 2314 48-49 0.8382540532299103 2314 50-51 0.899214899196501 2314 52-53 0.9879351801221077 2314 54-55 1.0545375250970963 2314 56-57 0.9333470100041481 2314 58-59 0.9176260601736814 2314 60-61 1.0018999086329359 2314 62-63 0.7354048210433319 2314 64-65 0.8565282378629533 2314 66-67 0.9751032411285081 2314 68-69 1.0224052181813548 2314 70-71 1.0975594434264693 2314 72-73 1.0594923362236628 2314 74-75 1.0605842479771326 2314 76-77 0.8784848910641081 2314 78-79 0.8456383028231826 2314 80-81 0.8470632124091679 2314 82-83 0.8750466217737376 2314 84-85 0.7895589397863461 2314 86-87 0.9332262488898344 2314 88-89 1.0299440974490928 2314 90-91 1.0796046908361383 2314 92-93 1.155217670742715 2314 94-95 1.2258389670719403 2314 96-97 1.2711586901643415 2314 98-99 1.2351894347122077 2314 100 1.3436787432566888 2315 1 -0.04125114809002284 2315 2 0.13586240708487907 2315 3 0.17695085434381497 2315 4 -0.017019274620132308 2315 5 0.26465954073621134 2315 6 0.3791346994793656 2315 7 0.43636926406504273 2315 8 0.35301455197240017 2315 9 0.5924717525615009 2315 10-11 0.5844476523495601 2315 12-13 0.6294332235408007 2315 14-15 0.6763332625259224 2315 16-17 0.7276946653046608 2315 18-19 0.7748865174304598 2315 20-21 0.7836099697018142 2315 22-23 0.8720453912304507 2315 24-25 0.8197568298150415 2315 26-27 0.9087507834971191 2315 28-29 0.9131724609188439 2315 30-31 0.8634121028632862 2315 32-33 0.9086368989903661 2315 34-35 0.9251050095590259 2315 36-37 0.9475792238707825 2315 38-39 0.9760023095973125 2315 40-41 0.9640552684779493 2315 42-43 1.0414107432492514 2315 44-45 0.8994612778162931 2315 46-47 0.9367844943406354 2315 48-49 1.0174989847193743 2315 50-51 0.9517348548058209 2315 52-53 0.9880618142656346 2315 54-55 0.926895327872721 2315 56-57 1.026135767435214 2315 58-59 1.0209253496341475 2315 60-61 1.0687106400900603 2315 62-63 1.0278937277863989 2315 64-65 1.0532047830433413 2315 66-67 1.0694119954384078 2315 68-69 1.0364053821183674 2315 70-71 0.6392272862791728 2315 72-73 0.8960277279400728 2315 74-75 1.0274893002719239 2315 76-77 0.9093436227029379 2315 78-79 0.7865235977463882 2315 80-81 0.8869719382895553 2315 82-83 0.8332330586402286 2315 84-85 0.8012706079421861 2315 86-87 0.7871506263265324 2315 88-89 0.9381360414472901 2315 90-91 1.0194005137242819 2315 92-93 0.9974237576093898 2315 94-95 1.0114218233356134 2315 96-97 1.1304064222218173 2315 98-99 1.0590198572760237 2315 100 1.012859038353639 2316 1 0.4826104362916972 2316 2 0.4722270767733292 2316 3 0.49382663258526804 2316 4 0.2975476800260566 2316 5 0.40864293579323885 2316 6 0.4214397140214672 2316 7 0.4679045363978602 2316 8 0.48515504492077355 2316 9 0.5512677545835132 2316 10-11 0.5127325985025664 2316 12-13 0.631040763832857 2316 14-15 0.7810585101999834 2316 16-17 0.7598036795633263 2316 18-19 0.8271124229959739 2316 20-21 0.8611289958696986 2316 22-23 0.8573657125817746 2316 24-25 0.9043651005707574 2316 26-27 0.9706827347359663 2316 28-29 0.918802694580279 2316 30-31 0.8251461993419085 2316 32-33 0.7583587077163614 2316 34-35 0.6820887544378067 2316 36-37 0.7005501272881105 2316 38-39 0.639032768324796 2316 40-41 0.6984930406401944 2316 42-43 0.8611988995700486 2316 44-45 0.8156163521152209 2316 46-47 0.8749242460251381 2316 48-49 0.6003216564356322 2316 50-51 0.6639738549794103 2316 52-53 0.8496100218374281 2316 54-55 0.9236414850359438 2316 56-57 0.9406252831875932 2316 58-59 0.8585928024338898 2316 60-61 0.9237149746661188 2316 62-63 0.978552402904981 2316 64-65 0.9802152703202935 2316 66-67 0.9792943217741694 2316 68-69 0.9823163530080947 2316 70-71 0.8412077897748915 2316 72-73 0.8994126551531778 2316 74-75 0.9480371730369086 2316 76-77 0.8243651283115447 2316 78-79 0.8252988501751943 2316 80-81 0.7597031545339306 2316 82-83 0.6763493277490475 2316 84-85 0.7354840713909496 2316 86-87 0.7890165948072685 2316 88-89 0.7524238565074874 2316 90-91 0.7693623639231255 2316 92-93 0.7506984764809808 2316 94-95 0.7592336987832624 2316 96-97 0.8893929822701168 2316 98-99 1.0108294999757739 2316 100 0.5994108547794035 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 9.0 12 36.0 13 236.0 14 901.0 15 2529.0 16 5893.0 17 11802.0 18 19043.0 19 27334.0 20 36650.0 21 48290.0 22 57790.0 23 60415.0 24 61362.0 25 67069.0 26 78843.0 27 95084.0 28 115894.0 29 141936.0 30 172900.0 31 214388.0 32 269115.0 33 347485.0 34 474846.0 35 659331.0 36 996846.0 37 1522534.0 38 1384280.0 39 214738.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.195097884628225 20.09601068774251 13.684974441710429 24.023916985918838 2 15.40990110632467 21.190635153278706 39.967678817632795 23.431784922763832 3 17.995454604456338 26.540377774629874 30.968385402586936 24.495782218326852 4 12.241568839950837 17.727583817704343 38.86745379601525 31.163393546329576 5 12.651971212984463 37.75346878006231 35.288038059859225 14.306521947093998 6 29.571840215271404 38.182625993441235 18.543751907377533 13.701781883909824 7 26.08508491306368 33.06239543360363 23.117466245988417 17.73505340734426 8 25.384623415997165 35.490227837928366 20.833282775423267 18.2918659706512 9 26.268097564059023 15.60664590989726 20.79467658988299 37.33057993616073 10-11 24.25686441123364 26.272595511638187 29.2484394295492 20.222100647578976 12-13 24.912045177572764 24.38249968695272 28.796357011465467 21.909098124009052 14-15 22.343762940714438 25.87256129039813 26.590031535308583 25.193644233578844 16-17 21.65656405182908 28.436824348538657 27.458545934564064 22.448065665068196 18-19 21.36080530367633 27.717403266431628 29.470652456708972 21.45113897318307 20-21 22.274358576228394 27.123502748422368 29.186771876534145 21.41536679881509 22-23 22.147678230693217 26.967383372566108 28.968120715411622 21.916817681329057 24-25 21.82557039021932 26.967145737826208 29.300085049898385 21.907198822056085 26-27 21.820785464891802 27.148948556223772 29.005272086372113 22.02499389251231 28-29 21.61286376534807 27.531006544446473 29.04440273742525 21.811726952780205 30-31 22.178855112537086 27.14767897662839 29.015128988462518 21.658336922372005 32-33 22.041370604290393 27.31449352625636 28.76289788680819 21.881237982645054 34-35 22.08400284617636 27.274033946947206 29.051172467166943 21.590790739709494 36-37 22.062036033142114 27.081995481955346 28.913940366665603 21.94202811823694 38-39 21.905636582591118 27.116952775390686 29.093069131438366 21.884341510579834 40-41 21.95962891230977 26.72425506134167 29.399837490760266 21.916278535588297 42-43 21.75133779720763 26.93649465914628 28.95058554223106 22.36158200141503 44-45 21.495548716061535 27.240366228377138 29.11429806698063 22.14978698858069 46-47 21.57120811623456 26.946896948001847 28.807709327810826 22.674185607952772 48-49 21.623969781043506 26.945050426776017 28.835767037706113 22.595212754474364 50-51 21.48811046995038 27.148614557323587 28.62167328346517 22.74160168926086 52-53 21.342870415552696 27.291655671603838 28.50341507827403 22.86205883456944 54-55 21.24146083997974 27.114846543112098 28.609256870661103 23.03443574624706 56-57 21.513726621173703 27.044382732388595 28.311406615349277 23.13048403108843 58-59 21.144585330144313 27.114865261954957 28.530959268618822 23.20959013928191 60-61 21.345279433097826 27.28459387274909 28.19717216285598 23.1729545312971 62-63 21.306205250243085 27.080156248886688 28.156171567886368 23.457466932983856 64-65 20.991962422013874 26.924097584958755 28.135460139044817 23.948479853982555 66-67 21.149989515753436 26.895900077331465 27.900884452592823 24.05322595432228 68-69 21.138266701375027 26.924274131298116 27.87557306909806 24.0618860982288 70-71 21.147298965736745 26.67815369691645 27.809484782484567 24.365062554862238 72-73 21.20318791061774 26.814622259381437 27.577971718016887 24.404218111983937 74-75 21.374993640293294 26.875700670321905 27.35299092507782 24.396314764306975 76-77 21.362348189708115 26.665708386636776 27.401438817242518 24.570504606412587 78-79 21.2613505405913 26.672357547020315 27.40204750339964 24.664244408988743 80-81 21.224477212622762 26.77292750137549 27.271709470755095 24.73088581524665 82-83 21.55165120060855 26.398943502579677 27.421590948783653 24.627814348028117 84-85 21.626643023678795 26.45949738512262 27.054823283863648 24.85903630733494 86-87 21.29644889089553 26.53895757371121 27.28085670024561 24.88373683514765 88-89 21.473381471280785 26.111448284741506 27.17742048196394 25.237749762013767 90-91 21.586055663362455 25.958868621756427 27.194825438212817 25.260250276668305 92-93 21.636937124470336 25.977398538631707 27.13023536426598 25.255428972631982 94-95 21.26247762127407 25.780412208794363 27.389697997259244 25.567412172672334 96-97 21.3839899358603 25.593783960192695 27.411947948892546 25.610278155054463 98-99 21.28132155138088 25.73740281148747 27.25121825878518 25.730057378346466 100 21.305654639980503 25.51729266839856 27.392920026039093 25.784132665581843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1470.0 1 1050.5 2 1150.0 3 1684.5 4 1313.0 5 1016.5 6 1450.5 7 2508.0 8 3880.5 9 4731.5 10 4450.0 11 3989.0 12 4248.0 13 4820.0 14 5546.0 15 6357.5 16 7135.5 17 7975.5 18 8946.0 19 9952.0 20 11217.0 21 12968.5 22 15258.5 23 18482.0 24 22500.5 25 27885.5 26 35326.5 27 44128.5 28 53740.5 29 66270.0 30 81250.5 31 95676.5 32 112562.5 33 128794.5 34 143404.0 35 158006.0 36 172482.5 37 190357.5 38 201844.0 39 204800.5 40 208384.5 41 213308.5 42 218351.0 43 226832.5 44 240124.5 45 257788.0 46 273204.5 47 283506.0 48 291511.0 49 293683.5 50 290677.5 51 287769.5 52 283112.0 53 272923.5 54 258504.0 55 233382.0 56 207033.0 57 179830.0 58 151328.5 59 128874.5 60 104235.0 61 81128.5 62 62490.0 63 46359.0 64 32657.0 65 22227.0 66 15548.0 67 11527.0 68 8548.0 69 6326.5 70 4780.0 71 3615.0 72 2709.5 73 1897.5 74 1300.0 75 842.0 76 536.0 77 323.5 78 199.5 79 121.5 80 76.0 81 45.0 82 25.5 83 17.0 84 11.5 85 12.0 86 9.5 87 5.0 88 2.5 89 2.5 90 4.0 91 2.0 92 1.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050919830261588805 2 0.0 3 0.0 4 0.015548255181011602 5 0.0035272811209191473 6 1.410912448367659E-5 7 1.4109124483676591E-4 8 4.232737345102977E-4 9 0.0017636405604595736 10-11 8.39492906778757E-4 12-13 3.4567354985007645E-4 14-15 1.410912448367659E-5 16-17 1.1287299586941272E-4 18-19 0.0 20-21 7.054562241838295E-6 22-23 0.0 24-25 1.1287299586941272E-4 26-27 7.054562241838295E-6 28-29 0.0010722934607594208 30-31 8.39492906778757E-4 32-33 0.013911596740905119 34-35 0.022772126916654013 36-37 0.03168909359033762 38-39 0.020676921930828044 40-41 2.1163686725514884E-4 42-43 0.0017142586247667056 44-45 0.009424895155095961 46-47 0.01377050549606835 48-49 0.005714195415889019 50-51 0.010236169812907365 52-53 0.015491818683076896 54-55 0.009918714512024642 56-57 0.0030475708884741435 58-59 0.008218565011741613 60-61 0.0015590582554462632 62-63 0.0010370206495502292 64-65 0.018842735747950087 66-67 0.04477530654894766 68-69 0.05395329202557928 70-71 0.04783698656190548 72-73 0.03927274800031379 74-75 0.028027775786823545 76-77 0.028225303529595017 78-79 0.011781118943869953 80-81 0.0020669867368586204 82-83 0.02729410131367236 84-85 0.04713858489996349 86-87 0.047413712827395184 88-89 0.059526396196631526 90-91 0.03404531737911161 92-93 0.025213005452330062 94-95 0.0073931812294465325 96-97 0.0038094636105926796 98-99 0.022243034748516143 100 0.018948554181577662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7087612.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.80286698268924 #Duplication Level Percentage of deduplicated Percentage of total 1 78.18038225704001 51.444932943158015 2 13.929454895308712 18.33196135234737 3 3.91689996796889 7.732297427302696 4 1.5615949494123549 4.11029698948082 5 0.7275358961353345 2.3936973899262517 6 0.4276813686921456 1.688559612901424 7 0.27515376860042984 1.267413478449982 8 0.18486212051207063 0.9731566020954919 9 0.1368249947939101 0.8103129239097734 >10 0.6172909082273914 6.927330309937557 >50 0.029380133634829306 1.3068802191681932 >100 0.011929697194613268 1.4026430313888036 >500 5.692113885463713E-4 0.245895501847171 >1k 3.622138394777595E-4 0.4831976522227121 >5k 0.0 0.0 >10k+ 7.761725119435667E-5 0.8814245658637655 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21657 0.3055613089429839 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18682 0.26358666360404603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11434 0.16132372934635814 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.821824896735318E-5 2 0.0 0.0 0.0 0.0 2.821824896735318E-5 3 0.0 0.0 0.0 0.0 2.821824896735318E-5 4 0.0 0.0 0.0 2.821824896735318E-5 2.821824896735318E-5 5 0.0 0.0 0.0 2.821824896735318E-5 4.232737345102977E-5 6 4.232737345102977E-5 0.0 0.0 2.821824896735318E-5 4.232737345102977E-5 7 4.232737345102977E-5 0.0 0.0 2.821824896735318E-5 4.232737345102977E-5 8 4.232737345102977E-5 0.0 0.0 2.821824896735318E-5 5.643649793470636E-5 9 4.232737345102977E-5 0.0 0.0 7.054562241838294E-5 5.643649793470636E-5 10-11 4.232737345102977E-5 0.0 0.0 8.465474690205953E-5 7.054562241838294E-5 12-13 4.232737345102977E-5 0.0 0.0 1.1992755811125101E-4 2.3280055398066372E-4 14-15 5.643649793470636E-5 7.054562241838295E-6 0.0 1.269821203530893E-4 3.0334617639904665E-4 16-17 9.876387138573613E-5 1.410912448367659E-5 0.0 1.9047318052963397E-4 3.59782674333753E-4 18-19 9.876387138573613E-5 1.410912448367659E-5 0.0 2.539642407061786E-4 4.162191722684594E-4 20-21 9.876387138573613E-5 1.410912448367659E-5 0.0 4.8676479468684236E-4 4.6560110796132744E-4 22-23 9.876387138573613E-5 1.410912448367659E-5 0.0 0.0016578221268319993 5.149830436541956E-4 24-25 9.876387138573613E-5 2.1163686725514884E-5 0.0 0.0043879377144234195 5.361467303797104E-4 26-27 9.876387138573613E-5 2.821824896735318E-5 0.0 0.009453113404063315 5.855286660725785E-4 28-29 9.876387138573613E-5 2.821824896735318E-5 0.0 0.027167119193319274 5.925832283144168E-4 30-31 9.876387138573613E-5 2.821824896735318E-5 0.0 0.08504980238760249 5.925832283144168E-4 32-33 1.0581843362757442E-4 2.821824896735318E-5 0.0 0.20915366134602176 6.066923527980933E-4 34-35 1.269821203530893E-4 2.821824896735318E-5 0.0 0.40040284372225793 6.631288507327997E-4 36-37 1.269821203530893E-4 2.821824896735318E-5 0.0 0.6953047091178242 6.631288507327997E-4 38-39 1.269821203530893E-4 2.821824896735318E-5 0.0 1.1050548478105178 7.125107864256678E-4 40-41 1.269821203530893E-4 2.821824896735318E-5 0.0 1.530402623619916 7.19565348667506E-4 42-43 1.269821203530893E-4 2.821824896735318E-5 0.0 1.9487734373721362 7.336744731511826E-4 44-45 1.340366825949276E-4 2.821824896735318E-5 0.0 2.4492381919326283 7.830564088440507E-4 46-47 1.410912448367659E-4 2.821824896735318E-5 0.0 3.0341248928411995 8.536020312624337E-4 48-49 1.410912448367659E-4 2.821824896735318E-5 0.0 3.627977942359147 9.029839669553017E-4 50-51 1.4814580707860417E-4 2.821824896735318E-5 0.0 4.222014410495383 9.735295893736847E-4 52-53 1.5520036932044248E-4 2.821824896735318E-5 0.0 4.789652706722659 0.0010229115250665528 54-55 1.5520036932044248E-4 2.821824896735318E-5 0.0 5.408782252753113 0.0010581843362757442 56-57 1.5520036932044248E-4 2.821824896735318E-5 0.0 6.11227025407147 0.0010652388985175824 58-59 1.5520036932044248E-4 2.821824896735318E-5 0.0 6.859045049305747 0.0010722934607594208 60-61 1.9047318052963397E-4 2.821824896735318E-5 0.0 7.60227845429462 0.0010722934607594208 62-63 1.9752774277147225E-4 2.821824896735318E-5 0.0 8.337596640448151 0.0011146208342104505 64-65 1.9752774277147225E-4 2.821824896735318E-5 0.0 9.05525020274812 0.0011146208342104505 66-67 2.1163686725514884E-4 2.821824896735318E-5 0.0 9.843209532350247 0.0011146208342104505 68-69 2.1163686725514884E-4 2.821824896735318E-5 0.0 10.697778038639813 0.0011146208342104505 70-71 2.1163686725514884E-4 2.821824896735318E-5 0.0 11.561390493723415 0.0011357845209359653 72-73 2.2574599173882544E-4 2.821824896735318E-5 0.0 12.387634932611999 0.0011640027699033185 74-75 2.2574599173882544E-4 2.821824896735318E-5 0.0 13.190020841998688 0.0011781118943869954 76-77 2.539642407061786E-4 2.821824896735318E-5 0.0 14.03736547655261 0.0012133847055961866 78-79 2.539642407061786E-4 2.821824896735318E-5 0.0 14.93523488588258 0.0012627666412890546 80-81 2.539642407061786E-4 2.821824896735318E-5 0.0 15.838183580026671 0.0012768757657727315 82-83 2.539642407061786E-4 4.232737345102977E-5 0.0 16.735107678016234 0.001312148576981923 84-85 2.539642407061786E-4 5.643649793470636E-5 0.0 17.592773137129967 0.001312148576981923 86-87 2.539642407061786E-4 5.643649793470636E-5 0.0 18.461810550577543 0.0013685850749166292 88 2.539642407061786E-4 5.643649793470636E-5 0.0 19.1248476920012 0.001439130697335012 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10990 0.0 54.54565 1 GTATCAA 20145 0.0 40.259598 1 TACCTGG 5705 0.0 38.549088 2 ATCAACG 24210 0.0 32.80363 3 CAACGCA 24665 0.0 32.098705 5 TATCAAC 25315 0.0 31.761131 2 TCAACGC 25600 0.0 31.023153 4 AACGCAG 26400 0.0 30.379883 6 ACCTGGG 7835 0.0 27.949686 3 ACGCAGA 29230 0.0 27.149218 7 CGCAGAG 29480 0.0 26.871168 8 GTACTAG 2365 0.0 25.446405 1 GTGGTAT 4675 0.0 24.438372 1 GTACATG 20715 0.0 24.171986 1 GTACCTG 9530 0.0 23.680779 1 GCAGAGT 32910 0.0 23.656534 9 TACATGG 20730 0.0 23.53 2 TATCACG 460 0.0 23.495998 2 ACATGGG 20260 0.0 23.032433 3 CATGGGG 14705 0.0 21.763126 4 >>END_MODULE