FastQCFastQC Report
Mon 6 Jun 2016
ERR1211400_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211400_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5282823
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56130.1062500106477162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65050.044.3333361
TACCTGG48600.040.973772
GTATCAA109300.038.585951
CAACGCA124700.032.4959535
ACCTGGG61950.031.8710563
ATCAACG128600.031.4739063
TATCAAC134650.030.6266542
AACGCAG138050.029.8988726
TCAACGC136650.029.6883134
GTACTAG19900.028.652241
GTACATG173200.027.6965831
TACATGG177600.026.4538782
ACGCAGA154450.025.9334757
CGCAGAG157600.025.174358
ACATGGG177550.024.8883233
GTACCTG84450.024.4399741
CATGGGG136700.023.7969864
CCTGGGG59950.023.6811124
TGGGGGG101900.022.8825386
CTAGTAC25400.022.7645933
Produced by FastQC (version 0.11.5)