FastQCFastQC Report
Mon 6 Jun 2016
ERR1211400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211400_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5282823
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65650.046.264361
TACCTGG49600.037.5173382
GTATCAA109600.036.849481
CAACGCA126500.030.8279325
ATCAACG127250.030.7250233
ACCTGGG59500.030.485763
TATCAAC136750.029.483462
TCAACGC135750.028.870954
AACGCAG139450.028.6718376
GTACTAG20300.026.6348691
GTACATG173300.026.1533471
TACATGG177850.024.9158842
TGGGGGG90400.024.6870756
ACGCAGA159250.024.6644447
CCTGGGG59100.023.8544124
ACATGGG177000.023.761643
CGCAGAG162950.023.6430748
CATGGGG137600.022.8477064
ATGGGGG95650.022.8415075
TCTAACG9250.022.352672
Produced by FastQC (version 0.11.5)