FastQCFastQC Report
Mon 6 Jun 2016
ERR1211399_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211399_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11181224
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383710.3431735201799016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306900.274477999904125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177910.15911495914937399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226900.046.729741
GTATCAA384800.038.208931
TACCTGG73700.031.6164992
CAACGCA459950.031.0193675
ATCAACG463800.030.8129653
GTACTAG52800.030.5237241
TATCAAC480400.030.4056872
AACGCAG494850.029.2028246
TCAACGC498050.028.8446484
ACGCAGA538250.026.525597
GTACATG417150.026.4795571
CGCAGAG545300.026.0939838
ACCTGGG89700.025.6799583
TACATGG425250.025.5486262
ACATGGG426350.024.7427123
GCAGAGT583150.023.7394379
CATGGGG309100.023.436274
TAGGCAT75600.022.8828875
GCATCGT57750.022.6285258
GAGTACT321400.022.51364512-13
Produced by FastQC (version 0.11.5)