FastQCFastQC Report
Mon 6 Jun 2016
ERR1211399_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211399_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11181224
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343010.30677321194888857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294810.2636652302109322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176810.15813116703502228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231350.045.2182121
GTATCAA385250.037.564611
CAACGCA458750.030.624315
ATCAACG462800.030.3399413
TATCAAC483950.029.5668182
TACCTGG71100.029.0143412
AACGCAG494750.028.8224226
TCAACGC491650.028.703484
GTACTAG55200.028.0226821
ACGCAGA541550.025.8891537
CGCAGAG547450.025.5500628
GTACATG414000.025.3254641
TACATGG423550.024.3416352
ACATGGG420450.023.6834033
ACCTGGG85600.023.4961933
GCAGAGT595200.023.1291879
CTAACGC24350.022.9654793
GGCGTCG47500.022.9483768
TAGGCAT74350.022.2450265
CTGTGCG69100.022.1664039
Produced by FastQC (version 0.11.5)