FastQCFastQC Report
Mon 6 Jun 2016
ERR1211398_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211398_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8214148
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316000.3847021017882804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248130.30207636872381655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157450.19168147445115427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA135900.051.698761
TACCTGG87350.049.3069842
GTATCAA248700.041.8544731
ACCTGGG102400.041.316773
ATCAACG304050.033.45773
CAACGCA305800.033.204545
GTACCTG134400.032.7468531
TATCAAC314350.032.7031362
TCAACGC317850.032.0938154
AACGCAG326800.031.401496
CCTGGGG96650.030.8369374
GTACATG262100.028.2982561
ACGCAGA359750.028.2642147
CGCAGAG367550.027.5346578
TAACGCA8900.027.4661084
TACATGG267150.027.310322
ACATGGG268800.026.1454283
GCAGAGT398900.024.5568439
CATGGGG188000.023.7797054
ATAACGC10150.023.620443
Produced by FastQC (version 0.11.5)