FastQCFastQC Report
Mon 6 Jun 2016
ERR1211398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211398_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8214148
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288750.3515276325676138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239600.291691846798962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158160.1925458367684634No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA129800.055.0916371
TACCTGG93100.051.8878442
ACCTGGG108000.043.7303543
GTATCAA240650.041.729821
GTACCTG143550.034.0613021
CCTGGGG98900.033.0243644
ATCAACG298000.032.9745183
CAACGCA302000.032.533025
TATCAAC314650.031.646482
TCAACGC313800.031.3595434
AACGCAG323000.030.926076
TAACGCA10200.029.9474054
CTGGGGG67400.029.0705595
ACGCAGA360100.027.3614627
ATAACGC11350.027.32683
CGCAGAG368650.026.6504048
GTACATG259700.025.0912551
GCAGAGT400250.024.0532979
TACATGG267700.023.8733142
TGGGGGG134250.023.5540076
Produced by FastQC (version 0.11.5)