FastQCFastQC Report
Mon 6 Jun 2016
ERR1211397_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211397_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205157
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333160.36192755865000453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263440.2861874056031853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162870.1769334298154828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159150.050.7780081
TACCTGG93550.045.8338932
GTATCAA281500.040.0908551
ACCTGGG108400.038.9828343
CAACGCA341750.032.4185875
ATCAACG343400.032.3312573
TATCAAC350200.031.8928812
TCAACGC357700.031.1044354
AACGCAG366550.030.5847726
GTACATG295350.029.037061
GTACCTG149900.029.0147131
CCTGGGG109650.028.2072184
TACATGG297700.028.0470352
ACGCAGA403400.027.395467
CGCAGAG409750.026.8330968
ACATGGG296650.026.699223
ATGGGGG183700.026.4834295
CATGGGG234000.026.1740884
TGGGGGG210450.025.1500916
GTACTAG30250.024.7679421
Produced by FastQC (version 0.11.5)