FastQCFastQC Report
Mon 6 Jun 2016
ERR1211397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211397_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9205157
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311160.33802791196282694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258920.2812771145565469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165440.1797253430875758No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158100.050.762851
TACCTGG91950.047.1722
ACCTGGG107350.039.7053453
GTATCAA277100.039.448531
ATCAACG342650.031.4620043
CAACGCA344250.031.3118655
TATCAAC355600.030.579892
CCTGGGG99950.030.467774
TCAACGC357200.030.2202474
AACGCAG370800.029.473146
GTACCTG151750.029.1544761
GTACATG301950.027.700351
TGGGGGG182150.027.1358246
CTGGGGG73100.027.0616465
TACATGG304850.026.899462
ACGCAGA409050.026.3265937
CGCAGAG416550.025.8300238
ACATGGG305500.025.6275753
CATGGGG229050.024.9078834
ATGGGGG173000.024.797865
Produced by FastQC (version 0.11.5)