##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211397_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9205157 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07488889108573 33.0 31.0 34.0 30.0 34.0 2 32.20996035157249 33.0 31.0 34.0 30.0 34.0 3 32.2854559677798 34.0 31.0 34.0 30.0 34.0 4 35.93069069870291 37.0 35.0 37.0 35.0 37.0 5 35.78943857231332 37.0 35.0 37.0 35.0 37.0 6 35.824700545574615 37.0 35.0 37.0 35.0 37.0 7 35.755868476767965 37.0 35.0 37.0 33.0 37.0 8 35.7416071230507 37.0 35.0 37.0 33.0 37.0 9 37.32902339416916 39.0 37.0 39.0 34.0 39.0 10-11 37.30518480021579 39.0 37.0 39.0 34.0 39.0 12-13 37.21785967365902 39.0 37.0 39.0 33.0 39.0 14-15 38.42643607273618 40.0 38.0 41.0 33.5 41.0 16-17 38.41309936375882 40.0 38.0 41.0 34.0 41.0 18-19 38.42080336055105 40.0 38.0 41.0 34.0 41.0 20-21 38.381369378056235 40.0 38.0 41.0 34.0 41.0 22-23 38.28323976440598 40.0 38.0 41.0 34.0 41.0 24-25 38.16730572873445 40.0 37.5 41.0 33.0 41.0 26-27 38.041530579000444 40.0 37.0 41.0 33.0 41.0 28-29 37.958500381905495 40.0 37.0 41.0 33.0 41.0 30-31 37.999046621366695 40.0 37.0 41.0 33.0 41.0 32-33 37.83444948304521 40.0 37.0 41.0 33.0 41.0 34-35 37.72311694412164 40.0 37.0 41.0 33.0 41.0 36-37 37.579160029535615 40.0 37.0 41.0 32.5 41.0 38-39 37.46335820236418 40.0 36.0 41.0 32.0 41.0 40-41 37.39579927860003 40.0 36.0 41.0 32.0 41.0 42-43 37.262866890809136 40.0 36.0 41.0 31.5 41.0 44-45 37.110728964209954 40.0 36.0 41.0 31.0 41.0 46-47 36.89198598133633 39.0 36.0 41.0 31.0 41.0 48-49 36.677105996128034 39.0 35.0 41.0 30.0 41.0 50-51 36.497325955439976 39.0 35.0 41.0 30.0 41.0 52-53 36.30048895418079 39.0 35.0 40.0 29.5 41.0 54-55 36.104795170793935 39.0 35.0 40.0 29.0 41.0 56-57 35.881662854854085 38.0 35.0 40.0 28.0 41.0 58-59 35.62895711610351 38.0 34.0 40.0 28.0 41.0 60-61 35.37452028248948 38.0 34.0 40.0 28.0 41.0 62-63 35.079735848068644 37.0 34.0 40.0 27.0 41.0 64-65 34.731582361930386 37.0 34.0 39.5 26.0 41.0 66-67 34.37979515178286 36.0 33.0 39.0 26.0 41.0 68-69 34.01635659228843 36.0 33.0 39.0 26.0 40.0 70-71 33.64088559271721 35.0 33.0 38.5 25.5 40.0 72-73 33.284074079344876 35.0 32.0 37.5 25.0 40.0 74-75 32.913565135282326 35.0 32.0 37.0 25.0 39.0 76-77 31.893898387610335 34.0 30.5 36.0 23.5 38.5 78-79 32.3756016871847 35.0 32.0 36.0 25.0 38.5 80-81 32.26938139132228 35.0 32.0 36.0 25.0 37.5 82-83 32.00111220264901 34.5 31.5 35.0 24.0 37.0 84-85 31.729561483850844 34.0 31.0 35.0 24.0 36.5 86-87 31.480994946636976 34.0 31.0 35.0 23.0 36.0 88-89 31.262098571485527 34.0 31.0 35.0 23.0 36.0 90-91 31.047839325282556 34.0 31.0 35.0 21.5 35.0 92-93 30.872349271174844 34.0 30.5 35.0 20.0 35.0 94-95 30.710891840302125 34.0 30.0 35.0 20.0 35.0 96-97 30.550035051004564 34.0 30.0 35.0 20.0 35.0 98-99 30.362069544278278 34.0 29.5 35.0 19.0 35.0 100 28.529836264606892 32.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2509289695973962 1101 2 -0.2829723698317217 1101 3 -0.3207787454114346 1101 4 -0.17833542544987324 1101 5 -0.18790709419503315 1101 6 -0.2857606599113609 1101 7 -0.2570959858369122 1101 8 -0.3340927822184767 1101 9 -0.4440058802295255 1101 10-11 -0.4021197841178932 1101 12-13 -0.4753777824933465 1101 14-15 -0.6744410913502747 1101 16-17 -0.6727468911860299 1101 18-19 -0.6429846900956449 1101 20-21 -0.7136721209235546 1101 22-23 -0.7029961211003908 1101 24-25 -0.8144897688458812 1101 26-27 -0.6790023809992789 1101 28-29 -0.7247533182408645 1101 30-31 -0.7092327484315604 1101 32-33 -0.626843451239175 1101 34-35 -0.679940908169641 1101 36-37 -0.6820836748924037 1101 38-39 -0.6849493868811791 1101 40-41 -0.7200434581814577 1101 42-43 -0.7276339805576342 1101 44-45 -0.711773032249404 1101 46-47 -0.7279394200177336 1101 48-49 -0.7696758979392087 1101 50-51 -0.8066508771956293 1101 52-53 -0.7974012312232119 1101 54-55 -0.7839260670310892 1101 56-57 -0.9225662297172761 1101 58-59 -0.8397503374503543 1101 60-61 -0.8466456637869797 1101 62-63 -0.8727866112750675 1101 64-65 -0.7955642656231134 1101 66-67 -0.8582531676026761 1101 68-69 -0.9426032834698574 1101 70-71 -0.9650289210166463 1101 72-73 -0.9895984258957284 1101 74-75 -1.0906612590676588 1101 76-77 -1.003533367561861 1101 78-79 -0.8523559773045406 1101 80-81 -0.7351161557850574 1101 82-83 -0.7632023468166658 1101 84-85 -0.7942700928433197 1101 86-87 -0.8785119101709213 1101 88-89 -0.893857902562786 1101 90-91 -0.9705867111741462 1101 92-93 -1.0516467159564513 1101 94-95 -1.0959360552440032 1101 96-97 -1.018518330256338 1101 98-99 -1.1061362549366223 1101 100 -1.1738452807300845 1102 1 -0.3206036363346243 1102 2 -0.27558806340256226 1102 3 -0.3241354470892972 1102 4 -0.14341111615692625 1102 5 -0.19790663464934255 1102 6 -0.30137477237313703 1102 7 -0.28868814740292237 1102 8 -0.29565907986906836 1102 9 -0.4658514827157205 1102 10-11 -0.4550755857869362 1102 12-13 -0.4867248331230769 1102 14-15 -0.6255539368099647 1102 16-17 -0.6267046944645713 1102 18-19 -0.6343302983209043 1102 20-21 -0.6672612785827496 1102 22-23 -0.6953568782304345 1102 24-25 -0.7812532493678717 1102 26-27 -0.7620500796136582 1102 28-29 -0.787179005575247 1102 30-31 -0.7015743683355922 1102 32-33 -0.682692323577264 1102 34-35 -0.7445502433883817 1102 36-37 -0.6950809486330414 1102 38-39 -0.7458729316100658 1102 40-41 -0.7825729251999789 1102 42-43 -0.7319948014831041 1102 44-45 -0.7354450240354922 1102 46-47 -0.8063467232260422 1102 48-49 -0.8462092105380989 1102 50-51 -0.8736166629327826 1102 52-53 -0.8491463633428751 1102 54-55 -0.8914891828428111 1102 56-57 -0.8842339175265153 1102 58-59 -0.9864139803745786 1102 60-61 -0.9775302959694301 1102 62-63 -1.0065437754193098 1102 64-65 -0.9470298947377316 1102 66-67 -1.0025751386279254 1102 68-69 -1.0804928481233986 1102 70-71 -1.0735483655664737 1102 72-73 -1.1204674385700102 1102 74-75 -1.0463582444773607 1102 76-77 -0.8945923364397146 1102 78-79 -0.8155241609788568 1102 80-81 -0.7390054343402284 1102 82-83 -0.6576404831162925 1102 84-85 -0.803893426687118 1102 86-87 -0.8190682385110044 1102 88-89 -0.8327561665045558 1102 90-91 -0.8139708978607523 1102 92-93 -0.9251568345597398 1102 94-95 -0.9200434914486841 1102 96-97 -0.9809170026387832 1102 98-99 -1.0098864490943242 1102 100 -1.0257108463351798 1103 1 -0.36112711928432617 1103 2 -0.3093262171211357 1103 3 -0.4044048318726965 1103 4 -0.21834991090918265 1103 5 -0.23531132492299633 1103 6 -0.36743290336103485 1103 7 -0.35005468967653286 1103 8 -0.39727981594997175 1103 9 -0.5287590403259657 1103 10-11 -0.5185693927344204 1103 12-13 -0.5968716639495213 1103 14-15 -0.7269763672904261 1103 16-17 -0.8054368198124351 1103 18-19 -0.7040538699776135 1103 20-21 -0.6870330094593768 1103 22-23 -0.7544035289131088 1103 24-25 -0.7902788191838894 1103 26-27 -0.879014219624878 1103 28-29 -0.8530918818204043 1103 30-31 -0.7529915584871958 1103 32-33 -0.759837884570409 1103 34-35 -0.842660357641499 1103 36-37 -0.7345629909426137 1103 38-39 -0.8130224656890377 1103 40-41 -0.8154292391686013 1103 42-43 -0.8576404528370531 1103 44-45 -0.8882091281414972 1103 46-47 -0.9748813778395586 1103 48-49 -1.0296772083678434 1103 50-51 -1.029777466508321 1103 52-53 -1.0969946404801618 1103 54-55 -0.9357886415347778 1103 56-57 -1.043104150446176 1103 58-59 -1.1013550645328607 1103 60-61 -1.1291473060370336 1103 62-63 -1.1154156920187788 1103 64-65 -1.1013979280144142 1103 66-67 -1.0932245588343577 1103 68-69 -1.1221236743279945 1103 70-71 -1.0588975119408062 1103 72-73 -1.0754829015251701 1103 74-75 -1.0528272610828253 1103 76-77 -0.9522948826218531 1103 78-79 -0.8843276863054861 1103 80-81 -0.8050055331692434 1103 82-83 -0.87559871151851 1103 84-85 -0.8361705647661992 1103 86-87 -0.922172555525794 1103 88-89 -0.9100929098374309 1103 90-91 -1.089529256892618 1103 92-93 -0.9857897806068685 1103 94-95 -1.0297759278289966 1103 96-97 -1.1236196255329638 1103 98-99 -1.231650391606923 1103 100 -1.2124821442371143 1104 1 -0.3628152476005191 1104 2 -0.29794749192411984 1104 3 -0.32570667843503287 1104 4 -0.17696949269477358 1104 5 -0.21430899716376928 1104 6 -0.3057469485886273 1104 7 -0.3484806863776626 1104 8 -0.3562549593256321 1104 9 -0.5098871765987809 1104 10-11 -0.4878628764613353 1104 12-13 -0.5430575276454164 1104 14-15 -0.6806526176647907 1104 16-17 -0.6890035157576904 1104 18-19 -0.7366255689848273 1104 20-21 -0.6902960350770257 1104 22-23 -0.7596991532174613 1104 24-25 -0.8580730885155816 1104 26-27 -0.869822266653884 1104 28-29 -0.8136471468251187 1104 30-31 -0.7215115763225484 1104 32-33 -0.7550298688314925 1104 34-35 -0.7460070144070841 1104 36-37 -0.7493572111041615 1104 38-39 -0.8130860376540099 1104 40-41 -0.8948336686161511 1104 42-43 -0.7782459831370332 1104 44-45 -0.8329162137824824 1104 46-47 -0.7628982929271899 1104 48-49 -0.8390047982933311 1104 50-51 -0.8225207497551565 1104 52-53 -0.8031787077896126 1104 54-55 -0.7249725512762737 1104 56-57 -0.828661887926792 1104 58-59 -0.8814465070283859 1104 60-61 -0.8435811872822043 1104 62-63 -0.9255119705095254 1104 64-65 -0.8861733713279492 1104 66-67 -0.9295096931358913 1104 68-69 -0.8304390857032828 1104 70-71 -0.8168646674033866 1104 72-73 -0.8797331993397748 1104 74-75 -0.8000594849589575 1104 76-77 -0.7348651568124467 1104 78-79 -0.7575065611361822 1104 80-81 -0.769193256390512 1104 82-83 -0.8072188565257328 1104 84-85 -0.7061636474212065 1104 86-87 -0.6183526082048978 1104 88-89 -0.7113847296020417 1104 90-91 -0.6743431981924068 1104 92-93 -0.8748115266609666 1104 94-95 -0.9366522325140174 1104 96-97 -1.0369903142055037 1104 98-99 -0.9891640363213519 1104 100 -1.0818434743788572 1105 1 -0.4033274403438263 1105 2 -0.37927162693303273 1105 3 -0.3999756868606923 1105 4 -0.24588356220261431 1105 5 -0.2673542214476541 1105 6 -0.2684727146228312 1105 7 -0.3509603936063641 1105 8 -0.3549704346161704 1105 9 -0.5458808253966367 1105 10-11 -0.4830547280017825 1105 12-13 -0.5742986085784807 1105 14-15 -0.8013413817617376 1105 16-17 -0.7521822326462555 1105 18-19 -0.6630317098209559 1105 20-21 -0.8089884020637825 1105 22-23 -0.7138644883374567 1105 24-25 -0.684989696135041 1105 26-27 -0.7039169806707264 1105 28-29 -0.7677739130069909 1105 30-31 -0.7330692682911746 1105 32-33 -0.7271994666456223 1105 34-35 -0.7568141341779651 1105 36-37 -0.849106740287624 1105 38-39 -0.7790761477518018 1105 40-41 -0.7255405697562551 1105 42-43 -0.8160855826371787 1105 44-45 -0.8682296442581077 1105 46-47 -0.9449449136821357 1105 48-49 -0.8631083666363821 1105 50-51 -0.9013142458751204 1105 52-53 -0.8574012764588517 1105 54-55 -0.9002442418960896 1105 56-57 -0.9245171803013861 1105 58-59 -0.9359718900730414 1105 60-61 -1.0072466372825346 1105 62-63 -0.9774225792816438 1105 64-65 -1.019316995992675 1105 66-67 -0.9990153688306336 1105 68-69 -0.9561376173838099 1105 70-71 -0.9686114408362201 1105 72-73 -1.0450067536657812 1105 74-75 -0.9909585103769842 1105 76-77 -0.8869121343300641 1105 78-79 -0.9303096675335567 1105 80-81 -0.8024145768036206 1105 82-83 -0.7656309898728786 1105 84-85 -0.7620720506010485 1105 86-87 -0.7159042726749725 1105 88-89 -0.7936548435787643 1105 90-91 -0.7964527730965933 1105 92-93 -0.7944742812435059 1105 94-95 -0.832930123366129 1105 96-97 -0.8509545081808163 1105 98-99 -0.8676440014109552 1105 100 -0.879167988465337 1106 1 -0.3970620050185545 1106 2 -0.3289984754415407 1106 3 -0.37228190677506845 1106 4 -0.1840269317785186 1106 5 -0.2864251217192546 1106 6 -0.2870976078478549 1106 7 -0.2870662059142788 1106 8 -0.3695021069935578 1106 9 -0.4885194329447202 1106 10-11 -0.5030747980474146 1106 12-13 -0.5602466788080562 1106 14-15 -0.7154930536923061 1106 16-17 -0.7623789635584828 1106 18-19 -0.742454597876474 1106 20-21 -0.6487456303571904 1106 22-23 -0.6732353434138574 1106 24-25 -0.7716897266443112 1106 26-27 -0.7250536020758602 1106 28-29 -0.7078670636010358 1106 30-31 -0.7252910533438524 1106 32-33 -0.6405220961717859 1106 34-35 -0.6322785631071994 1106 36-37 -0.7831692811167486 1106 38-39 -0.8183443671871586 1106 40-41 -0.7755957329284584 1106 42-43 -0.7483150222891837 1106 44-45 -0.804305114242446 1106 46-47 -0.8442500072861989 1106 48-49 -0.8651288084179001 1106 50-51 -0.8538840839142949 1106 52-53 -0.8452526064298809 1106 54-55 -0.9176031863312417 1106 56-57 -0.9278322369373555 1106 58-59 -0.9524252203218779 1106 60-61 -1.039826169392093 1106 62-63 -0.9943682478325044 1106 64-65 -0.970194803281963 1106 66-67 -0.9039271958435293 1106 68-69 -0.9775195698104611 1106 70-71 -0.924160065743294 1106 72-73 -0.9286421850627704 1106 74-75 -0.9310664283376369 1106 76-77 -0.9427425865565056 1106 78-79 -0.8641652684224539 1106 80-81 -0.7590802620557113 1106 82-83 -0.7808874576333409 1106 84-85 -0.8221466277026259 1106 86-87 -0.8803398160107712 1106 88-89 -0.8760424908611881 1106 90-91 -0.8600640259168131 1106 92-93 -0.892547986853284 1106 94-95 -0.7968723674352454 1106 96-97 -0.9102552779868063 1106 98-99 -0.984158971842426 1106 100 -0.9838050555924909 1107 1 -0.3749753122369981 1107 2 -0.3336718683324591 1107 3 -0.4281801243047063 1107 4 -0.1977128231079135 1107 5 -0.21592085030864183 1107 6 -0.2791211210194007 1107 7 -0.33684187049356495 1107 8 -0.3440512552587194 1107 9 -0.5018438193808947 1107 10-11 -0.46589370573037314 1107 12-13 -0.5476981670148362 1107 14-15 -0.5874027277167855 1107 16-17 -0.6162489851022102 1107 18-19 -0.6882422981958953 1107 20-21 -0.6233007106524511 1107 22-23 -0.6728145182196172 1107 24-25 -0.6873025856106452 1107 26-27 -0.6921936447446058 1107 28-29 -0.7241811932082172 1107 30-31 -0.7648751412663017 1107 32-33 -0.7601395639765585 1107 34-35 -0.7568255479260912 1107 36-37 -0.7466609508017683 1107 38-39 -0.8190389381234198 1107 40-41 -0.8111907050793192 1107 42-43 -0.7988744127277272 1107 44-45 -0.8430761650980898 1107 46-47 -0.8976173416203039 1107 48-49 -0.8445401277083704 1107 50-51 -0.8285033697118322 1107 52-53 -0.8535089455019289 1107 54-55 -0.8335478900402862 1107 56-57 -0.9227907092372192 1107 58-59 -0.9178767280694515 1107 60-61 -0.9328191507536587 1107 62-63 -0.8812275100694009 1107 64-65 -0.9882761544448826 1107 66-67 -0.9898025785467084 1107 68-69 -0.8363313213714605 1107 70-71 -0.859967648831848 1107 72-73 -0.9096982469345463 1107 74-75 -0.8094877266985137 1107 76-77 -0.8170634563456538 1107 78-79 -0.5848458607042559 1107 80-81 -0.6997678122598785 1107 82-83 -0.6832301907917859 1107 84-85 -0.6607378333633172 1107 86-87 -0.736226727788921 1107 88-89 -0.6797376570941225 1107 90-91 -0.6645465819099421 1107 92-93 -0.7281648028326408 1107 94-95 -0.7399836753501781 1107 96-97 -0.7919665849246975 1107 98-99 -0.7966270871806316 1107 100 -0.8322368013052035 1108 1 -0.45968191038049255 1108 2 -0.43150760388222054 1108 3 -0.4651712136259931 1108 4 -0.2837632773407037 1108 5 -0.3467107503467517 1108 6 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0.6540063872348583 2116 74-75 0.5886910100765519 2116 76-77 0.49414905070642945 2116 78-79 0.4867274244653572 2116 80-81 0.44434958893210563 2116 82-83 0.40726004788998793 2116 84-85 0.345117433736327 2116 86-87 0.3656606622824974 2116 88-89 0.3765281830454086 2116 90-91 0.29326892734859555 2116 92-93 0.45162434148986463 2116 94-95 0.5229822191280853 2116 96-97 0.5831485255727209 2116 98-99 0.6790035897712592 2116 100 0.30723374408738024 2201 1 0.6804822756655469 2201 2 0.6313461178326918 2201 3 0.6129146294204091 2201 4 0.3965287801139894 2201 5 0.5001710326430313 2201 6 0.5265402315712677 2201 7 0.5622036676017999 2201 8 0.5512955355110094 2201 9 0.7155791923642525 2201 10-11 0.6396375653570985 2201 12-13 0.6473248821507482 2201 14-15 0.8409598647535717 2201 16-17 0.8148604825078323 2201 18-19 0.7838932989397023 2201 20-21 0.8286526465732109 2201 22-23 0.7970571160494657 2201 24-25 0.7653619404895764 2201 26-27 0.7825295966356407 2201 28-29 0.7993202697486268 2201 30-31 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1.0800920491012533 2202 58-59 1.0626863372176132 2202 60-61 1.0698833147215936 2202 62-63 1.0578555939687746 2202 64-65 1.1628244623168413 2202 66-67 1.1727475246324133 2202 68-69 1.1339823318690279 2202 70-71 1.1443330624650159 2202 72-73 1.1388863058220196 2202 74-75 1.0919789616744637 2202 76-77 1.140233600758851 2202 78-79 1.0329575109502045 2202 80-81 0.9901836183847479 2202 82-83 0.9604338642971761 2202 84-85 0.8338435209741348 2202 86-87 0.9042099219545285 2202 88-89 0.9809992870189461 2202 90-91 0.9946543885875627 2202 92-93 0.9474061962300162 2202 94-95 0.963494686985694 2202 96-97 0.8211876476133604 2202 98-99 0.8535440427722705 2202 100 1.0849843103960097 2203 1 0.6730948912065742 2203 2 0.6408985063762032 2203 3 0.6206375214113962 2203 4 0.364521956728602 2203 5 0.46501973225185367 2203 6 0.4888569442719799 2203 7 0.5118279022180872 2203 8 0.507823999322973 2203 9 0.6842111457139808 2203 10-11 0.675568289346792 2203 12-13 0.7015838019431513 2203 14-15 0.8482001737592881 2203 16-17 0.8594479036231135 2203 18-19 0.8212303198800441 2203 20-21 0.8336013635945463 2203 22-23 0.7830468342734989 2203 24-25 0.815779558473082 2203 26-27 0.8497520635385172 2203 28-29 0.8371971014770665 2203 30-31 0.9170715495207702 2203 32-33 0.9881046266323139 2203 34-35 0.9996383742825969 2203 36-37 0.941601807443206 2203 38-39 0.9567072286737144 2203 40-41 0.9417112638439775 2203 42-43 1.020034024019509 2203 44-45 1.050469007585761 2203 46-47 1.0566280474334633 2203 48-49 1.0611237725228904 2203 50-51 0.9987144013522453 2203 52-53 1.0502360715876122 2203 54-55 1.03166178670633 2203 56-57 1.0390591029187917 2203 58-59 1.01164614875686 2203 60-61 1.0243502599420324 2203 62-63 1.0115393944517521 2203 64-65 1.0390456579915082 2203 66-67 1.0318276017658192 2203 68-69 0.9985257829443555 2203 70-71 1.0365234613099972 2203 72-73 1.1101350988569223 2203 74-75 1.068093810404953 2203 76-77 1.0886018243196283 2203 78-79 0.9490421173263499 2203 80-81 0.9112143822861256 2203 82-83 0.8693234341640554 2203 84-85 0.8937428888870613 2203 86-87 0.836289056846578 2203 88-89 0.9073583514756152 2203 90-91 0.9288045154415521 2203 92-93 1.0173548787794964 2203 94-95 0.9945300226167006 2203 96-97 0.9513508823161168 2203 98-99 0.9517080181280022 2203 100 1.1485421495448662 2204 1 0.7220069584952711 2204 2 0.6700931466825537 2204 3 0.6425879514633692 2204 4 0.39102216187706773 2204 5 0.5195552989175098 2204 6 0.5198336029353214 2204 7 0.5737471800954879 2204 8 0.5779685530110896 2204 9 0.7245086869716175 2204 10-11 0.7277693804327114 2204 12-13 0.7673978424561838 2204 14-15 0.9389981443090889 2204 16-17 0.8688393724861427 2204 18-19 0.8740856066320077 2204 20-21 0.8908623466356005 2204 22-23 0.9051974360423358 2204 24-25 0.9179038708458123 2204 26-27 0.9189177551167944 2204 28-29 0.9932342029571757 2204 30-31 1.0441025383363751 2204 32-33 1.0530733604863656 2204 34-35 1.0816100030094589 2204 36-37 1.0786407932499742 2204 38-39 1.107115341155854 2204 40-41 1.1134664039527848 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68-69 1.2589627532176024 2205 70-71 1.240603800384541 2205 72-73 1.2090526227639273 2205 74-75 1.2176763506882438 2205 76-77 1.1621472616360258 2205 78-79 1.0200646081548541 2205 80-81 0.9752879080910191 2205 82-83 0.9693965519515153 2205 84-85 0.976664471740115 2205 86-87 1.0347016348432199 2205 88-89 1.0710019464124656 2205 90-91 1.0914803559610213 2205 92-93 1.1151513281741963 2205 94-95 1.1092213253046488 2205 96-97 1.1268156667012938 2205 98-99 1.1053855775772234 2205 100 1.370944888136318 2206 1 0.6557056571214375 2206 2 0.6361501143945745 2206 3 0.599540012213474 2206 4 0.29541661727095914 2206 5 0.46528170608230823 2206 6 0.4683032568684311 2206 7 0.5529159239364247 2206 8 0.5509526553819626 2206 9 0.7416833435132659 2206 10-11 0.6815991584165957 2206 12-13 0.7455969685937944 2206 14-15 0.9134779933033457 2206 16-17 0.8993918042292322 2206 18-19 0.8473352733179951 2206 20-21 0.8884102786395474 2206 22-23 0.8747173914072519 2206 24-25 0.947302963652028 2206 26-27 0.9686671174725987 2206 28-29 0.9814409011195977 2206 30-31 1.0474408593032791 2206 32-33 1.093182557054682 2206 34-35 1.1221163504675857 2206 36-37 1.1383246521567045 2206 38-39 1.1181043780232827 2206 40-41 1.1225617100220049 2206 42-43 1.1985615412832047 2206 44-45 1.136132552053354 2206 46-47 1.1767898868939355 2206 48-49 1.1698404744171427 2206 50-51 1.134330203238541 2206 52-53 1.1491469914042014 2206 54-55 1.1206337603075198 2206 56-57 1.1046832497081027 2206 58-59 1.1588743063398326 2206 60-61 1.1418212757142001 2206 62-63 1.1223853827202035 2206 64-65 1.144198637459077 2206 66-67 1.2005120078210112 2206 68-69 1.2709004285223031 2206 70-71 1.281927028292678 2206 72-73 1.2473157282312783 2206 74-75 1.2720433217692388 2206 76-77 1.1973056675750904 2206 78-79 1.077719148366029 2206 80-81 1.042367213032641 2206 82-83 1.0028721773000306 2206 84-85 1.0339513089991499 2206 86-87 1.0661189886016338 2206 88-89 1.0758707569543873 2206 90-91 1.1450642288312345 2206 92-93 1.2007355738352565 2206 94-95 1.1705516498422703 2206 96-97 1.1557362661786819 2206 98-99 1.2211146814560117 2206 100 1.4647969651143953 2207 1 0.6949288163725527 2207 2 0.6592642598938525 2207 3 0.642024101411522 2207 4 0.39786149605374277 2207 5 0.5181751570448512 2207 6 0.5072090322674399 2207 7 0.560294504078513 2207 8 0.584299160805628 2207 9 0.72657727714423 2207 10-11 0.7229983514330272 2207 12-13 0.7701381075572726 2207 14-15 0.9387542937791764 2207 16-17 0.9223281418198823 2207 18-19 0.8816201370695467 2207 20-21 0.8803382994403322 2207 22-23 0.8919871258200018 2207 24-25 0.9436042889619713 2207 26-27 0.9354546445826202 2207 28-29 0.9669675445032837 2207 30-31 1.002041004103802 2207 32-33 1.051996472344392 2207 34-35 1.0160278213036023 2207 36-37 1.0486991989660694 2207 38-39 1.0774323804006727 2207 40-41 1.071321366002401 2207 42-43 1.1103415743876752 2207 44-45 1.1525197179870403 2207 46-47 1.1404489134600126 2207 48-49 1.2217026395096298 2207 50-51 1.2216190754292597 2207 52-53 1.1661109781719219 2207 54-55 1.165412636528039 2207 56-57 1.2121035751933533 2207 58-59 1.0524287653740032 2207 60-61 1.0939055815834635 2207 62-63 1.2416694607575778 2207 64-65 1.2723905832512088 2207 66-67 1.2587670708499843 2207 68-69 1.3129898098313006 2207 70-71 1.3342868979181617 2207 72-73 1.3304322411402936 2207 74-75 1.284174076639168 2207 76-77 1.3165275342345346 2207 78-79 1.2357409432700592 2207 80-81 1.1146177376166335 2207 82-83 1.0876914806939944 2207 84-85 1.1110421489769493 2207 86-87 1.131938459837972 2207 88-89 1.1719530441758934 2207 90-91 1.2516734287263596 2207 92-93 1.3178439873677554 2207 94-95 1.3103896421375865 2207 96-97 1.2697487191177892 2207 98-99 1.271601450476151 2207 100 1.4300913921349512 2208 1 0.7199170781012754 2208 2 0.6660772578457852 2208 3 0.6258727192870097 2208 4 0.3990803232279134 2208 5 0.4852811431951807 2208 6 0.5048418111904809 2208 7 0.5683951045795368 2208 8 0.53569633209176 2208 9 0.7282276128627956 2208 10-11 0.6996732559017502 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1.2848640506819926 2310 78-79 1.122979016589646 2310 80-81 1.08557576642324 2310 82-83 1.1414744715933658 2310 84-85 1.1975109621700568 2310 86-87 1.227391096262064 2310 88-89 1.2510916799997744 2310 90-91 1.2707382999049806 2310 92-93 1.3346578349317788 2310 94-95 1.3617820131644613 2310 96-97 1.3999880331593992 2310 98-99 1.347791874075778 2310 100 1.415230712068226 2311 1 0.4046528245339971 2311 2 0.4885339717305257 2311 3 0.5124056734239488 2311 4 0.354790622252672 2311 5 -0.009191357864864358 2311 6 0.13158137896881072 2311 7 0.38590835225665643 2311 8 0.19529303408556586 2311 9 0.36292342038634473 2311 10-11 0.4638794740428054 2311 12-13 0.4991187637306709 2311 14-15 0.7006502389076061 2311 16-17 0.8358070740966994 2311 18-19 0.8628804493554227 2311 20-21 0.926101713749965 2311 22-23 0.8536354993018307 2311 24-25 0.8191168047373196 2311 26-27 0.8544640804845471 2311 28-29 0.8347225889098482 2311 30-31 0.8744163283823099 2311 32-33 1.004383102331822 2311 34-35 0.9739242363951277 2311 36-37 1.0007957877263962 2311 38-39 1.0417167351322476 2311 40-41 0.9942518057203316 2311 42-43 1.0465094326561157 2311 44-45 1.0511142380208867 2311 46-47 1.1101194964736862 2311 48-49 1.1452496031527843 2311 50-51 1.1281425422912221 2311 52-53 1.1757621819834796 2311 54-55 1.1640621611411888 2311 56-57 1.1698129815807548 2311 58-59 1.1694042342115054 2311 60-61 1.2209707262505063 2311 62-63 1.1696336925775128 2311 64-65 1.1866318776060112 2311 66-67 1.2042005886707514 2311 68-69 1.2046544249714302 2311 70-71 1.2001163755378954 2311 72-73 1.1723736208516087 2311 74-75 1.0842523634701493 2311 76-77 0.9922299422334611 2311 78-79 0.8962627007614472 2311 80-81 0.9328309364102978 2311 82-83 0.9485987535371656 2311 84-85 1.0042898316643445 2311 86-87 1.0771688891781217 2311 88-89 1.0581963129827088 2311 90-91 1.0825228973023933 2311 92-93 1.1676338608231696 2311 94-95 1.1355249769441862 2311 96-97 1.2032085352504218 2311 98-99 1.1802597289918957 2311 100 1.3098198943229207 2312 1 0.08008779055871429 2312 2 0.10089205144412006 2312 3 0.28583426997445116 2312 4 0.22039870109693283 2312 5 0.29404426889504975 2312 6 0.3658811029894906 2312 7 0.4134756274063207 2312 8 0.2587340553474533 2312 9 0.37390339891631896 2312 10-11 0.2771361968385335 2312 12-13 0.3942241110261264 2312 14-15 0.739905421885048 2312 16-17 0.8062339652654416 2312 18-19 0.7996782017657296 2312 20-21 0.8281829079046759 2312 22-23 0.4462513908894792 2312 24-25 0.40046692920797256 2312 26-27 0.7229137052984314 2312 28-29 0.8894610740346494 2312 30-31 0.7946864443257056 2312 32-33 0.6316630107900565 2312 34-35 0.8661978531932633 2312 36-37 0.9398212620394659 2312 38-39 0.8820263056373463 2312 40-41 0.9168316757221433 2312 42-43 0.7888558303165709 2312 44-45 0.7578484832164776 2312 46-47 0.9132696758435657 2312 48-49 0.9518839514121638 2312 50-51 0.9310530963980099 2312 52-53 0.9802196864616164 2312 54-55 1.0086222214707234 2312 56-57 0.9896246346869049 2312 58-59 1.0617936141490034 2312 60-61 1.0818309506958101 2312 62-63 1.0550335381060236 2312 64-65 1.0561392138133172 2312 66-67 1.0691205953656748 2312 68-69 1.1146802659653545 2312 70-71 0.9548624868367099 2312 72-73 1.069674116697513 2312 74-75 1.0976327106538264 2312 76-77 1.0439933368536636 2312 78-79 0.9143974134662542 2312 80-81 0.7905748881100507 2312 82-83 0.8007037984468717 2312 84-85 0.6511624208193467 2312 86-87 0.819540541831504 2312 88-89 0.509522295028475 2312 90-91 0.8393793484548198 2312 92-93 0.9969920831072727 2312 94-95 1.0435118786598814 2312 96-97 1.0737042757701012 2312 98-99 1.122672658966362 2312 100 1.291168039731783 2313 1 -0.4142733305769326 2313 2 -0.06755067406755444 2313 3 0.21025981054241782 2313 4 0.0996823066225403 2313 5 0.22865864816307635 2313 6 0.3293791970617548 2313 7 0.08798471900368554 2313 8 0.07030707526727298 2313 9 0.27011732982576575 2313 10-11 0.3569079913228208 2313 12-13 0.5727990418057303 2313 14-15 0.6777704161988751 2313 16-17 0.7765556532114104 2313 18-19 0.689831422692194 2313 20-21 0.8595418320703274 2313 22-23 0.9145867518163797 2313 24-25 0.7320637154921812 2313 26-27 0.9537490191621316 2313 28-29 0.8559868270795619 2313 30-31 0.8325876223641302 2313 32-33 1.00630373540087 2313 34-35 0.988026366087503 2313 36-37 0.8449195120361779 2313 38-39 0.5858667107941997 2313 40-41 0.7175648940355543 2313 42-43 0.7643212837014985 2313 44-45 0.9838278923448911 2313 46-47 0.9961018350330093 2313 48-49 1.0322903793616547 2313 50-51 0.9454977051874423 2313 52-53 0.9326673316923078 2313 54-55 0.9419815336927257 2313 56-57 1.0528799490681706 2313 58-59 0.9521990342218771 2313 60-61 1.0157823861712316 2313 62-63 1.0776994668216489 2313 64-65 1.1599595985812883 2313 66-67 1.1690459186251516 2313 68-69 1.1838244862094953 2313 70-71 1.063332635246887 2313 72-73 1.1573225027209375 2313 74-75 0.8834831638771661 2313 76-77 0.9859661865043456 2313 78-79 0.8119360181135562 2313 80-81 0.9357672663889716 2313 82-83 0.9131129070483262 2313 84-85 0.9096925903605211 2313 86-87 1.0211759996736838 2313 88-89 0.9906177512852423 2313 90-91 1.0902324318511099 2313 92-93 0.79742997875017 2313 94-95 0.8240748767323254 2313 96-97 1.1439068644218047 2313 98-99 1.200204738992511 2313 100 1.2155017793675071 2314 1 -0.35661160465823016 2314 2 -0.14858314910206616 2314 3 0.09654900955014512 2314 4 -0.16622615856445577 2314 5 0.1866626626769161 2314 6 0.21242338392650595 2314 7 0.17106044755553285 2314 8 0.20042618711278948 2314 9 0.2694195897255298 2314 10-11 0.4489316243789361 2314 12-13 0.5165495909151261 2314 14-15 0.5924364222882801 2314 16-17 0.7402208356305309 2314 18-19 0.8295104928498134 2314 20-21 0.4282158367160349 2314 22-23 0.6733192846787048 2314 24-25 0.8505230441103251 2314 26-27 0.8581793367068684 2314 28-29 0.8096098192043613 2314 30-31 0.8241979339267473 2314 32-33 0.8803149311365175 2314 34-35 0.9019839414513626 2314 36-37 0.8550298038883497 2314 38-39 0.8847075459357612 2314 40-41 0.9332492316069434 2314 42-43 0.7511323936607042 2314 44-45 0.7708783242552499 2314 46-47 0.9071271228242281 2314 48-49 0.7550101368067885 2314 50-51 0.9083625381815139 2314 52-53 1.0533773864003564 2314 54-55 1.039246422607242 2314 56-57 0.8924442751107193 2314 58-59 0.8743340363812635 2314 60-61 0.9941041925704539 2314 62-63 0.7587302313561111 2314 64-65 0.8599870666645373 2314 66-67 0.9148515706830693 2314 68-69 0.9798476281833715 2314 70-71 1.098902806369182 2314 72-73 1.1034688767941603 2314 74-75 1.0933851841514226 2314 76-77 0.894247279693861 2314 78-79 0.8427412265828877 2314 80-81 0.842114452340958 2314 82-83 0.8755424889126111 2314 84-85 0.7740036379129336 2314 86-87 0.8836114442190031 2314 88-89 0.9715751545569162 2314 90-91 1.0587041483605724 2314 92-93 1.085215503846964 2314 94-95 1.204891248944019 2314 96-97 1.2732489353003906 2314 98-99 1.2009237238673087 2314 100 1.1889453976051385 2315 1 -0.10130392421037726 2315 2 0.10715469658169496 2315 3 0.11171547033541174 2315 4 -0.07940698217434061 2315 5 0.22180322975120959 2315 6 0.34283791456493873 2315 7 0.4305278761949154 2315 8 0.3424531417079635 2315 9 0.5771270661593348 2315 10-11 0.5789363105108336 2315 12-13 0.6422790083638077 2315 14-15 0.7457543578733876 2315 16-17 0.7353363640500419 2315 18-19 0.856739563695939 2315 20-21 0.7768866749363852 2315 22-23 0.8974611195742739 2315 24-25 0.8426179394437057 2315 26-27 0.9186949904749326 2315 28-29 0.9302244757959883 2315 30-31 0.9449280083178664 2315 32-33 0.9852676905547852 2315 34-35 0.9451522112292849 2315 36-37 0.9669234458726521 2315 38-39 0.961874531962799 2315 40-41 0.9500880263940559 2315 42-43 1.0254001238339114 2315 44-45 0.8663624987201359 2315 46-47 0.8921748368580111 2315 48-49 1.0260903755255342 2315 50-51 0.9363777382349667 2315 52-53 0.9673611478742501 2315 54-55 0.9251464678024846 2315 56-57 1.0380092260152693 2315 58-59 1.0124457898442998 2315 60-61 1.0748049809580706 2315 62-63 1.034817769722828 2315 64-65 1.073821925293096 2315 66-67 1.0737147568684478 2315 68-69 1.003488753241804 2315 70-71 0.6192202701370988 2315 72-73 0.8650406038383736 2315 74-75 1.0178410057397045 2315 76-77 0.991109258761373 2315 78-79 0.8811045085307825 2315 80-81 0.9260074108389418 2315 82-83 0.9046060077500151 2315 84-85 0.8003190029976537 2315 86-87 0.8149489565984389 2315 88-89 0.9076943847174874 2315 90-91 0.9864146919223771 2315 92-93 1.040084449601526 2315 94-95 1.0893189100351321 2315 96-97 1.1293967585797624 2315 98-99 1.0037280592815705 2315 100 0.9235059337562674 2316 1 0.5385056219610647 2316 2 0.5180578264251281 2316 3 0.5187937266532074 2316 4 0.34861652800231013 2316 5 0.4110728611472254 2316 6 0.4322139958934059 2316 7 0.47845246153383414 2316 8 0.4859368081954756 2316 9 0.5196072670104357 2316 10-11 0.47816940426898924 2316 12-13 0.6486015958187963 2316 14-15 0.8161874852359787 2316 16-17 0.8654344451867928 2316 18-19 0.8686321214275239 2316 20-21 0.9107878369819034 2316 22-23 0.9256892721168484 2316 24-25 0.9416808569186159 2316 26-27 1.030714031549529 2316 28-29 0.9715040721981651 2316 30-31 0.881208622546545 2316 32-33 0.8905347542276374 2316 34-35 0.7791782769223587 2316 36-37 0.7233502034723287 2316 38-39 0.6746629924310952 2316 40-41 0.7703905237130613 2316 42-43 0.9571852479136069 2316 44-45 0.9280749891207947 2316 46-47 0.9733969610673086 2316 48-49 0.6503162978253414 2316 50-51 0.7817027910919379 2316 52-53 0.8687089512179611 2316 54-55 1.0058504878043024 2316 56-57 0.9972292801054792 2316 58-59 0.9948437460055999 2316 60-61 1.0267292115835502 2316 62-63 1.0955353392034013 2316 64-65 1.0756934694848965 2316 66-67 1.0066095092996505 2316 68-69 0.9867766164775134 2316 70-71 0.850705252080104 2316 72-73 0.8713547183813688 2316 74-75 0.9415112342606093 2316 76-77 0.7958948743417587 2316 78-79 0.7985007252319747 2316 80-81 0.8132749577550058 2316 82-83 0.7019575406110867 2316 84-85 0.7684322039740508 2316 86-87 0.7663707363623296 2316 88-89 0.6819492980191271 2316 90-91 0.761444625356166 2316 92-93 0.8091467962319498 2316 94-95 0.6932385293859689 2316 96-97 0.8679964734447339 2316 98-99 0.9647874533175731 2316 100 0.6624737535647149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 12.0 12 48.0 13 300.0 14 1124.0 15 3123.0 16 7470.0 17 14844.0 18 24729.0 19 34734.0 20 46893.0 21 61518.0 22 73896.0 23 77941.0 24 79634.0 25 86236.0 26 100114.0 27 120907.0 28 148665.0 29 180610.0 30 220352.0 31 272212.0 32 343439.0 33 446379.0 34 619272.0 35 853096.0 36 1282196.0 37 1992372.0 38 1830242.0 39 282761.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11118758070927 19.517500264388925 13.27669341786243 24.094618737039372 2 15.865975995846677 20.919969099929528 39.93864526156371 23.275409642660087 3 18.109979004160383 26.720739255180547 31.08003481092175 24.08924692973732 4 12.455807439463566 17.217831200417656 38.17006211051902 32.156299249599755 5 12.56271209126057 37.848607525407495 34.58391763221741 15.00476275111453 6 29.649380233275764 37.27847336009587 18.96237076673434 14.109775639894028 7 26.347508022691667 33.26322033124971 22.877957315152187 17.511314330906426 8 25.955017632582223 35.35834087080687 20.655444069266686 18.03119742734422 9 27.17742129978575 15.607419190061394 20.525934074481743 36.68922543567111 10-11 24.79568342603941 26.017009307254103 29.008895959382418 20.178411307324065 12-13 25.173994078191104 24.077257384784446 28.591709195957275 22.157039341067176 14-15 22.776195662984257 25.636598494897616 26.572541503784645 25.014664338333485 16-17 21.983803739417812 27.97593347071658 27.383335816544857 22.656926973320747 18-19 21.636534824989948 27.381504736964292 29.294329254786206 21.687631183259555 20-21 22.58009900865868 26.870646223265453 28.894773031783444 21.65448173629242 22-23 22.325052268258556 26.679921194169808 28.79816872206355 22.196857815508082 24-25 21.92064649024478 26.531467356237144 29.134385146663274 22.4135010068548 26-27 21.9772996761054 26.68963205942757 28.914944941176984 22.418123323290047 28-29 21.753473689562277 27.472429819531747 28.850357640487495 21.923738850418477 30-31 22.7526584245475 26.722658237694073 28.980803634864067 21.54387970289436 32-33 22.58193966560884 26.77486438796767 28.66808457677524 21.975111369648253 34-35 22.590252901265035 26.94895930419079 28.908745126301227 21.552042668242947 36-37 22.254697585472773 26.81880915228218 28.85724266072819 22.06925060151686 38-39 22.20429631132309 26.940965338190427 28.84882167710575 22.005916673380735 40-41 22.247457508540876 26.396409442372654 29.2338990025577 22.122234046528767 42-43 22.012470628699283 26.73557241621907 28.66270128079458 22.589255674287063 44-45 21.46970763339521 27.083403500072684 29.055592265586416 22.391296600945694 46-47 21.522517173691973 26.527465928452287 28.73984913129376 23.210167766561977 48-49 21.72792173489107 26.60574250138178 28.82565670200674 22.840679061720415 50-51 21.526897363201726 26.875579561621898 28.728378650728935 22.869144424447438 52-53 21.336448612854756 27.196834610025338 28.283222065201503 23.183494711918406 54-55 21.142576899555173 26.845596386103065 28.7182210559973 23.293605658344458 56-57 21.5750479705699 26.692560589579696 28.2329855551889 23.499405884661503 58-59 21.133544351483685 26.813879612097296 28.580791307742697 23.47178472867632 60-61 21.629397340995236 26.990236743569245 28.192178335676076 23.188187579759443 62-63 21.534794154340464 26.89064113923528 28.003204110090852 23.571360596333406 64-65 21.097008911191345 26.606517329356823 28.046457107944466 24.250016651507366 66-67 21.196625023611837 26.573151478541106 27.838425266066537 24.39179823178052 68-69 20.912429926919867 26.68937889169588 27.991330035810723 24.40686114557353 70-71 21.042943204968683 26.45893818098346 28.02776516999208 24.470353444055775 72-73 21.09958969690215 26.76118231760852 27.653989729897244 24.48523825559209 74-75 21.396832761975755 26.77908361289965 27.388961529193566 24.43512209593102 76-77 21.148947053086875 26.583297061228745 27.489883247742757 24.777872637941623 78-79 20.947257547970686 26.619982517346706 27.766111629636764 24.666648305045843 80-81 21.060283630735448 26.76897127706618 27.42193435572762 24.748810736470762 82-83 21.32219211110448 26.476778634167957 27.611063678765774 24.589965575961788 84-85 21.424653264189597 26.676493415690505 27.159259089788137 24.739594230331765 86-87 21.019571865310475 26.786356320679744 27.396671498206217 24.797400315803568 88-89 21.017055236303865 26.30137185832747 27.522947098570615 25.158625806798053 90-91 21.47893023550748 26.01939198498744 27.450537495295123 25.051140284209954 92-93 21.330247926268832 26.0784159997818 27.438580225935382 25.15275584801398 94-95 20.926846433886013 25.8818687768613 27.678986891027492 25.512297898225196 96-97 20.844044415568316 25.83034753853016 27.785721971891842 25.539886074009686 98-99 20.85086669602612 25.92068014832717 27.592881321640405 25.63557183400631 100 20.75532503826009 25.92525307072181 27.704041580037064 25.61538031098104 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2705.0 1 1949.5 2 2559.5 3 3827.5 4 2785.0 5 2039.5 6 2811.5 7 4887.0 8 7423.0 9 8603.5 10 7588.5 11 6490.5 12 6869.0 13 7656.0 14 8581.0 15 9597.0 16 10662.0 17 11863.0 18 13165.5 19 14369.0 20 15820.0 21 17878.0 22 20586.5 23 24463.0 24 29766.0 25 36115.0 26 44309.5 27 54287.0 28 66122.0 29 81185.0 30 98278.5 31 114580.5 32 133441.5 33 151598.5 34 168505.0 35 186690.5 36 206557.5 37 231253.5 38 247039.0 39 251682.5 40 256890.5 41 264121.5 42 274916.5 43 289744.0 44 307431.0 45 330321.5 46 351011.0 47 369605.5 48 389556.0 49 403605.0 50 411578.0 51 413655.0 52 403547.5 53 380395.5 54 351437.0 55 313120.5 56 273834.5 57 236040.5 58 198324.5 59 165345.0 60 129073.0 61 97374.0 62 75487.0 63 57460.5 64 41252.5 65 28452.5 66 20143.0 67 14992.0 68 11222.5 69 8241.0 70 6127.5 71 4614.5 72 3478.0 73 2450.0 74 1737.5 75 1218.0 76 813.5 77 496.0 78 297.5 79 172.5 80 99.0 81 70.5 82 57.0 83 37.5 84 25.0 85 19.5 86 10.0 87 7.5 88 3.5 89 1.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05101488220135735 2 0.0 3 0.0 4 0.015230593025192292 5 0.004269345976391278 6 0.0 7 1.8467908803728171E-4 8 4.2367555490905803E-4 9 0.0017707465500045247 10-11 8.582145855850151E-4 12-13 3.3133601089041715E-4 14-15 2.715868941724731E-5 16-17 8.147606825174194E-5 18-19 0.0 20-21 2.715868941724731E-5 22-23 5.431737883449462E-6 24-25 7.0612592484843E-5 26-27 2.172695153379785E-5 28-29 0.0011189380039905892 30-31 8.799415371188128E-4 32-33 0.013421824310003622 34-35 0.022373328341928334 36-37 0.03037970998213284 38-39 0.020026817576278166 40-41 1.41225184969686E-4 42-43 0.0018250639288390192 44-45 0.009679356908306942 46-47 0.014410400604791422 48-49 0.005915162555076464 50-51 0.010885202718432723 52-53 0.01617028367902905 54-55 0.00988576294787802 56-57 0.003199293613351733 58-59 0.007990086426554159 60-61 0.0014557057527644559 62-63 0.001140664955524387 64-65 0.01940216771968148 66-67 0.04631099719429011 68-69 0.05561013245075559 70-71 0.04880959662067686 72-73 0.04115627794289657 74-75 0.02889141380206769 76-77 0.02957581277538232 78-79 0.012780879239756585 80-81 0.0021238095124287396 82-83 0.027984313575531626 84-85 0.04801656288969324 86-87 0.04889650442681206 88-89 0.059955522757515166 90-91 0.03544208968950774 92-93 0.02695771511555968 94-95 0.0075827060852954485 96-97 0.0034926074590580043 98-99 0.021596589824595062 100 0.01848963575526197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9205157.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.471266161170945 #Duplication Level Percentage of deduplicated Percentage of total 1 75.43905459829097 46.37334204158651 2 15.128445394028677 18.59929386842155 3 4.605174281409956 8.492576819133918 4 1.8680684736900697 4.593301374139785 5 0.9234904211188324 2.8384062736943796 6 0.5096984319202709 1.8799084782301467 7 0.33424629851571225 1.4382580225611883 8 0.22631822342771304 1.1129654199558643 9 0.1510784587934766 0.8358285736541968 >10 0.763619809803599 8.145950659568149 >50 0.033557774085296144 1.4109465234248073 >100 0.015084054847946794 1.6659547395988072 >500 0.001298384193496155 0.5313635985119203 >1k 7.355865018907115E-4 0.8771649822949666 >5k 6.490468602568023E-5 0.22655262323167893 >10k+ 6.490468602568023E-5 0.9781860019921602 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31116 0.33802791196282694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25892 0.2812771145565469 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16544 0.1797253430875758 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0863475766898925E-5 0.0 2 0.0 0.0 0.0 1.0863475766898925E-5 0.0 3 0.0 0.0 0.0 1.0863475766898925E-5 0.0 4 0.0 0.0 0.0 3.259042730069677E-5 0.0 5 0.0 2.172695153379785E-5 0.0 3.259042730069677E-5 0.0 6 0.0 2.172695153379785E-5 0.0 3.259042730069677E-5 1.0863475766898925E-5 7 0.0 2.172695153379785E-5 0.0 3.259042730069677E-5 1.0863475766898925E-5 8 0.0 2.172695153379785E-5 0.0 3.259042730069677E-5 3.259042730069677E-5 9 0.0 2.172695153379785E-5 0.0 7.604433036829248E-5 5.431737883449462E-5 10-11 0.0 2.172695153379785E-5 0.0 1.4122518496968603E-4 6.518085460139354E-5 12-13 0.0 2.715868941724731E-5 0.0 1.7924735015383225E-4 8.69078061351914E-5 14-15 0.0 3.259042730069677E-5 0.0 2.2270125322142794E-4 1.2492997131933762E-4 16-17 0.0 3.259042730069677E-5 0.0 2.444282047552258E-4 1.6295213650348387E-4 18-19 0.0 3.259042730069677E-5 0.0 3.3676774877386666E-4 1.9554256380418063E-4 20-21 0.0 3.259042730069677E-5 0.0 7.278528763822278E-4 2.0640603957107956E-4 22-23 0.0 3.259042730069677E-5 0.0 0.0021129460366618408 2.3899646687177632E-4 24-25 0.0 3.802216518414624E-5 0.0 0.0055512361168853505 2.82450369939372E-4 26-27 0.0 4.34539030675957E-5 0.0 0.010771136222880283 2.82450369939372E-4 28-29 0.0 4.34539030675957E-5 0.0 0.030336256079065246 2.82450369939372E-4 30-31 0.0 4.34539030675957E-5 0.0 0.093827840198706 2.8788210782282146E-4 32-33 0.0 4.34539030675957E-5 0.0 0.22841000973693332 3.150407972400688E-4 34-35 0.0 4.34539030675957E-5 0.0 0.4294223335897476 3.3676774877386666E-4 36-37 0.0 4.888564095104516E-5 0.0 0.7374073033192156 3.3676774877386666E-4 38-39 0.0 5.431737883449462E-5 0.0 1.1981761962343498 3.476312245407656E-4 40-41 0.0 5.431737883449462E-5 0.0 1.7059187583655553 3.584947003076645E-4 42-43 0.0 5.431737883449462E-5 0.0 2.1836020830497516 3.6935817607456343E-4 44-45 0.0 5.431737883449462E-5 0.0 2.7377479819192656 4.128120791421591E-4 46-47 0.0 5.431737883449462E-5 0.0 3.3541198699815764 4.3453903067595695E-4 48-49 0.0 5.431737883449462E-5 0.0 4.00236519594397 4.3453903067595695E-4 50-51 0.0 5.431737883449462E-5 0.0 4.66282650040624 4.562659822097548E-4 52-53 0.0 5.974911671794408E-5 0.0 5.303016559087476 4.671294579766537E-4 54-55 0.0 8.69078061351914E-5 0.0 6.007458645192038 4.834246716270021E-4 56-57 0.0 8.69078061351914E-5 0.0 6.762046535436603 4.888564095104516E-4 58-59 0.0 8.69078061351914E-5 0.0 7.586052035831654 4.94288147393901E-4 60-61 0.0 8.69078061351914E-5 0.0 8.395511342174826 5.051516231608E-4 62-63 0.0 8.69078061351914E-5 0.0 9.207040140651593 5.431737883449462E-4 64-65 0.0 8.69078061351914E-5 0.0 9.985332135019533 5.431737883449462E-4 66-67 0.0 8.69078061351914E-5 0.0 10.831270992987953 5.649007398787441E-4 68-69 0.0 8.69078061351914E-5 0.0 11.752488306283098 5.86627691412542E-4 70-71 0.0 8.69078061351914E-5 0.0 12.704786023747342 5.974911671794409E-4 72-73 0.0 8.69078061351914E-5 0.0 13.577334965606779 6.300815944801376E-4 74-75 0.0 8.69078061351914E-5 0.0 14.405696719784356 6.409450702470366E-4 76-77 0.0 8.69078061351914E-5 0.0 15.293275280367297 6.409450702470366E-4 78-79 0.0 8.69078061351914E-5 0.0 16.248755996231242 6.409450702470366E-4 80-81 0.0 8.69078061351914E-5 0.0 17.171602830891423 6.572402838973849E-4 82-83 0.0 8.69078061351914E-5 0.0 18.0910385341608 6.952624490815312E-4 84-85 0.0 8.69078061351914E-5 0.0 18.980664859925803 6.952624490815312E-4 86-87 0.0 9.777128190209032E-5 0.0 19.882903681056174 7.006941869649806E-4 88 0.0 9.777128190209032E-5 0.0 20.56117022230039 7.061259248484301E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15810 0.0 50.76285 1 TACCTGG 9195 0.0 47.172 2 ACCTGGG 10735 0.0 39.705345 3 GTATCAA 27710 0.0 39.44853 1 ATCAACG 34265 0.0 31.462004 3 CAACGCA 34425 0.0 31.311865 5 TATCAAC 35560 0.0 30.57989 2 CCTGGGG 9995 0.0 30.46777 4 TCAACGC 35720 0.0 30.220247 4 AACGCAG 37080 0.0 29.47314 6 GTACCTG 15175 0.0 29.154476 1 GTACATG 30195 0.0 27.70035 1 TGGGGGG 18215 0.0 27.135824 6 CTGGGGG 7310 0.0 27.061646 5 TACATGG 30485 0.0 26.89946 2 ACGCAGA 40905 0.0 26.326593 7 CGCAGAG 41655 0.0 25.830023 8 ACATGGG 30550 0.0 25.627575 3 CATGGGG 22905 0.0 24.907883 4 ATGGGGG 17300 0.0 24.79786 5 >>END_MODULE