FastQCFastQC Report
Mon 6 Jun 2016
ERR1211396_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211396_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT379970.38814388322239207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300310.3067702438890348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189070.19313725820685226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170200.051.703321
TACCTGG88850.045.8732152
GTATCAA310950.040.8593561
ACCTGGG103800.038.131783
ATCAACG378500.033.1105653
CAACGCA383800.032.6288385
TATCAAC392150.032.2608452
TCAACGC401400.031.3387074
AACGCAG410700.030.9040816
GTACATG368750.029.2808511
TACATGG373950.028.1924742
CCTGGGG103000.028.0679194
ACGCAGA453800.027.5963067
GTACTAG38850.027.404291
ACATGGG373200.027.2702183
CGCAGAG458850.027.2085428
CATGGGG283800.027.0486814
GTACCTG153550.026.9367871
ATGGGGG222900.026.1507385
TGGGGGG246650.025.805626
Produced by FastQC (version 0.11.5)