FastQCFastQC Report
Mon 6 Jun 2016
ERR1211396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211396_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9789411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339480.34678286569028516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287910.29410349611432185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189950.19403618869408998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA173850.053.8961561
TACCTGG86850.044.0978782
GTATCAA304150.039.3855361
ACCTGGG102850.036.462113
ATCAACG376150.031.0961783
CAACGCA377900.030.9225655
TATCAAC386650.030.8341732
TCAACGC398150.029.555144
AACGCAG404250.029.3823076
CCTGGGG94000.028.2465884
GTACATG360600.027.3790721
TGGGGGG214650.027.361386
TACATGG365450.026.5277962
CTGGGGG70700.026.251515
ACGCAGA448350.026.1149837
GTACTAG39050.025.8846681
CGCAGAG454100.025.7117488
ACATGGG363500.025.6883743
ATGGGGG209550.025.5619415
CATGGGG273350.025.3581964
Produced by FastQC (version 0.11.5)