FastQCFastQC Report
Mon 6 Jun 2016
ERR1211395_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211395_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302496
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295980.4053134709009084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235280.3221912069517053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146560.20069850089613198No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78480.10747010337287416No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130200.051.0167121
TACCTGG79650.050.984792
ACCTGGG95050.042.233493
ATAACGC8150.041.5265733
GTATCAA234300.041.4189721
TAACGCA8550.037.3847124
CAACGCA288050.033.04525
ATCAACG288350.033.027123
TATCAAC293350.032.7395362
CCTGGGG92900.032.0792924
TCAACGC297350.032.07494
AACGCAG307700.031.3323066
GTACCTG133500.030.8767191
GTACATG258150.029.9826351
TACATGG258200.029.2141272
ACGCAGA340600.028.0023577
ACATGGG260350.027.8044533
CATGGGG203350.027.3689884
CGCAGAG349000.027.2600988
ATGGGGG162650.027.2237575
Produced by FastQC (version 0.11.5)