##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211395_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7302496 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.338466327129794 31.0 31.0 34.0 28.0 34.0 2 31.374783224804233 31.0 31.0 34.0 28.0 34.0 3 31.438187162307244 31.0 31.0 34.0 28.0 34.0 4 35.07420366953984 37.0 35.0 37.0 32.0 37.0 5 35.13258439306232 37.0 35.0 37.0 32.0 37.0 6 35.19549671783456 37.0 35.0 37.0 32.0 37.0 7 35.1280968863249 37.0 35.0 37.0 32.0 37.0 8 35.12780219256539 37.0 35.0 37.0 32.0 37.0 9 36.69515395831781 39.0 37.0 39.0 32.0 39.0 10-11 36.619025056638165 39.0 37.0 39.0 32.0 39.0 12-13 36.52223075507334 39.0 37.0 39.0 32.0 39.0 14-15 37.698821129104346 40.0 37.0 41.0 32.0 41.0 16-17 37.67871019717094 40.0 37.0 41.0 32.0 41.0 18-19 37.683932110335974 40.0 37.0 41.0 32.0 41.0 20-21 37.66636962211277 40.0 37.0 41.0 32.0 41.0 22-23 37.544058839607715 40.0 37.0 41.0 32.0 41.0 24-25 37.451896721340205 40.0 37.0 41.0 31.5 41.0 26-27 37.31449897404942 40.0 36.5 41.0 31.0 41.0 28-29 37.166691840707614 40.0 36.0 41.0 31.0 41.0 30-31 36.99712386011578 39.0 36.0 41.0 30.0 41.0 32-33 36.775229044973116 39.0 35.5 41.0 30.0 41.0 34-35 36.648811379013424 39.0 35.0 41.0 30.0 41.0 36-37 36.52123301402698 39.0 35.0 41.0 30.0 41.0 38-39 36.36134590145616 39.0 35.0 40.5 29.5 41.0 40-41 36.19360243401708 39.0 35.0 40.0 29.0 41.0 42-43 35.9981187939028 38.5 35.0 40.0 28.0 41.0 44-45 35.86609564729649 38.0 35.0 40.0 27.5 41.0 46-47 35.640439652414734 38.0 34.5 40.0 26.5 41.0 48-49 35.389885184462955 38.0 34.0 40.0 26.0 41.0 50-51 34.71774842464823 37.5 33.5 39.5 25.0 40.5 52-53 35.02084759786243 38.0 34.0 39.5 26.0 40.5 54-55 35.432183393185014 38.0 34.0 40.0 26.0 41.0 56-57 35.357162879651014 38.0 34.0 40.0 26.0 41.0 58-59 35.15842706384228 38.0 34.0 40.0 26.0 41.0 60-61 34.934808591473384 38.0 34.0 40.0 26.0 41.0 62-63 34.641152490874354 37.0 33.5 40.0 25.0 41.0 64-65 34.43923858362949 37.0 33.0 40.0 25.0 41.0 66-67 34.23759478950759 36.0 33.0 39.5 25.0 41.0 68-69 33.9014702644137 36.0 33.0 39.0 24.0 41.0 70-71 33.53236393419456 35.0 33.0 39.0 24.0 41.0 72-73 33.147532912034464 35.0 32.0 38.0 23.0 40.0 74-75 32.77652305458298 35.0 32.0 37.0 22.5 39.5 76-77 32.389541808718555 35.0 31.5 37.0 21.5 39.0 78-79 31.99748538034119 35.0 31.0 36.0 20.0 39.0 80-81 31.60853515017331 35.0 31.0 36.0 20.0 38.0 82-83 31.238634844853046 35.0 30.5 35.0 19.0 37.0 84-85 30.89699658856369 34.0 30.0 35.0 18.0 37.0 86-87 30.557517183165864 34.0 30.0 35.0 17.5 36.0 88-89 30.240615674421456 34.0 29.0 35.0 15.5 36.0 90-91 29.941249676822828 34.0 29.0 35.0 13.0 36.0 92-93 29.644259784599676 34.0 29.0 35.0 10.5 35.0 94-95 29.350128093188957 34.0 28.0 35.0 10.0 35.0 96-97 29.037726210325893 34.0 27.0 35.0 10.0 35.0 98-99 28.711129181036185 34.0 26.5 35.0 10.0 35.0 100 27.455999291201255 32.0 24.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3478615536816463 1101 2 -0.3293075163029364 1101 3 -0.4311104061048283 1101 4 -0.30423137352469354 1101 5 -0.29581726431744215 1101 6 -0.2869331192435425 1101 7 -0.3438964023504525 1101 8 -0.3418629476559829 1101 9 -0.4552485593815305 1101 10-11 -0.4369981145455384 1101 12-13 -0.5489848804421911 1101 14-15 -0.7105172508452284 1101 16-17 -0.707253941056301 1101 18-19 -0.6646592582704045 1101 20-21 -0.6976417467562186 1101 22-23 -0.6628924972010353 1101 24-25 -0.6945183036331741 1101 26-27 -0.6979849436589305 1101 28-29 -0.8201579812698938 1101 30-31 -0.7761203791610214 1101 32-33 -0.7759235798542576 1101 34-35 -0.7307719661486303 1101 36-37 -0.8111871258418475 1101 38-39 -0.866175323736357 1101 40-41 -1.047287127413071 1101 42-43 -1.0709745525504601 1101 44-45 -1.0319757774241296 1101 46-47 -1.0550619847826965 1101 48-49 -1.1503294083000597 1101 50-51 -1.0280859050793865 1101 52-53 -0.8244951746392957 1101 54-55 -0.7634716932075136 1101 56-57 -0.818609656343277 1101 58-59 -0.7411088321063488 1101 60-61 -0.7317012553759881 1101 62-63 -0.8551178198413965 1101 64-65 -0.8134897792069893 1101 66-67 -0.9751274888861801 1101 68-69 -0.9006291520998317 1101 70-71 -0.8783766813955722 1101 72-73 -0.9668293483120394 1101 74-75 -1.0710817156742714 1101 76-77 -0.9916032771145673 1101 78-79 -1.073199207071898 1101 80-81 -1.1631885955480357 1101 82-83 -1.0520773196678377 1101 84-85 -1.1374315861822204 1101 86-87 -1.205673519329686 1101 88-89 -1.3035951448156027 1101 90-91 -1.415103249229798 1101 92-93 -1.4759610195530293 1101 94-95 -1.5289003063628925 1101 96-97 -1.625705086219618 1101 98-99 -1.7789446257067318 1101 100 -1.771846385581398 1102 1 -0.3218965842830599 1102 2 -0.3442961599405727 1102 3 -0.44643378598272676 1102 4 -0.35156356434493574 1102 5 -0.3017336307262397 1102 6 -0.36480118599690314 1102 7 -0.4343218340003716 1102 8 -0.3502999774149771 1102 9 -0.5709430808346667 1102 10-11 -0.5228240174755925 1102 12-13 -0.4774984751984306 1102 14-15 -0.669614953608658 1102 16-17 -0.6635649982799094 1102 18-19 -0.7190645600546546 1102 20-21 -0.8109645357749287 1102 22-23 -0.734175724018435 1102 24-25 -0.7332056823960684 1102 26-27 -0.7728588648870272 1102 28-29 -0.7649730832556472 1102 30-31 -0.7972609467524236 1102 32-33 -0.7275052444215291 1102 34-35 -0.8028588726584971 1102 36-37 -0.832518211211621 1102 38-39 -0.9169054544020625 1102 40-41 -0.9956479709084363 1102 42-43 -1.0076530925740457 1102 44-45 -0.8994962553164711 1102 46-47 -1.0724134354236696 1102 48-49 -1.107719226567859 1102 50-51 -0.97682886995036 1102 52-53 -0.7810717580085935 1102 54-55 -0.7975713786761887 1102 56-57 -0.7878125323907952 1102 58-59 -0.7468291430605518 1102 60-61 -0.9132702358415941 1102 62-63 -0.7997282884035144 1102 64-65 -0.9326668475116833 1102 66-67 -0.9225808049726609 1102 68-69 -0.9490705720416699 1102 70-71 -0.8984579458168795 1102 72-73 -0.945600503646773 1102 74-75 -1.0908751295139787 1102 76-77 -1.0455608089145088 1102 78-79 -1.040597321864496 1102 80-81 -1.0959455052866929 1102 82-83 -1.0911278396925503 1102 84-85 -1.1315806946510953 1102 86-87 -1.2226497109702876 1102 88-89 -1.3809113984208459 1102 90-91 -1.5176930662119865 1102 92-93 -1.365788885991055 1102 94-95 -1.4294279187801067 1102 96-97 -1.6372147444915726 1102 98-99 -1.6714392849980548 1102 100 -1.690555079402973 1103 1 -0.45080891220782604 1103 2 -0.5323125173758072 1103 3 -0.5353605191222286 1103 4 -0.3853107049530422 1103 5 -0.2885819029584056 1103 6 -0.3465286849645679 1103 7 -0.4082827397806028 1103 8 -0.440688781753785 1103 9 -0.52632839330726 1103 10-11 -0.5128915417507827 1103 12-13 -0.6375537483307312 1103 14-15 -0.7144730156762762 1103 16-17 -0.7533863602865196 1103 18-19 -0.7926070296099823 1103 20-21 -0.7638398231214865 1103 22-23 -0.7309864997279405 1103 24-25 -0.7788973270337536 1103 26-27 -0.8841076685620024 1103 28-29 -0.8357172072174137 1103 30-31 -0.9359216995318533 1103 32-33 -0.9811347068641822 1103 34-35 -0.9002131022751669 1103 36-37 -1.0024156291131305 1103 38-39 -0.9425848010594109 1103 40-41 -0.9288458141884703 1103 42-43 -0.9890267774721977 1103 44-45 -1.0466646236158326 1103 46-47 -1.1433525652624112 1103 48-49 -1.1585723570134192 1103 50-51 -1.0816186218646138 1103 52-53 -0.9009647713893827 1103 54-55 -0.8578613411562515 1103 56-57 -0.8501890127184666 1103 58-59 -0.831535271230571 1103 60-61 -0.8714932103351387 1103 62-63 -0.8511590618350979 1103 64-65 -0.8556150377828331 1103 66-67 -0.8669727286849209 1103 68-69 -0.9080895690818593 1103 70-71 -0.9820068176898573 1103 72-73 -0.9788394918157692 1103 74-75 -1.02212304913229 1103 76-77 -1.0472065256599628 1103 78-79 -1.1279908044077978 1103 80-81 -1.1309205974517376 1103 82-83 -1.065296327652561 1103 84-85 -1.1833173188129358 1103 86-87 -1.2852386824016584 1103 88-89 -1.3692487859698979 1103 90-91 -1.2879485135525393 1103 92-93 -1.4881348864047546 1103 94-95 -1.4445525610873133 1103 96-97 -1.5298578843221797 1103 98-99 -1.6280775440072013 1103 100 -1.7721690921559983 1104 1 -0.39248618898277243 1104 2 -0.35599775172625314 1104 3 -0.3339036065037 1104 4 -0.13102416904165892 1104 5 -0.17495959771247982 1104 6 -0.2070710011850352 1104 7 -0.21766644908328914 1104 8 -0.3096008930001872 1104 9 -0.45661364771237345 1104 10-11 -0.42061168949366134 1104 12-13 -0.4668150611271358 1104 14-15 -0.635839553452854 1104 16-17 -0.6512494053919013 1104 18-19 -0.6486892164465701 1104 20-21 -0.6386801917206384 1104 22-23 -0.6739978640146589 1104 24-25 -0.623786530270273 1104 26-27 -0.6350283085465804 1104 28-29 -0.7757517059000492 1104 30-31 -0.7926834442985822 1104 32-33 -0.8423865327855893 1104 34-35 -0.8585558321864752 1104 36-37 -0.9159194386653837 1104 38-39 -0.9084832021397631 1104 40-41 -0.8766706405144689 1104 42-43 -0.8591701783717198 1104 44-45 -0.9512444389964045 1104 46-47 -0.9297703259359764 1104 48-49 -0.9462961953810449 1104 50-51 -0.8794920324302566 1104 52-53 -0.6545365657497086 1104 54-55 -0.6910847865982319 1104 56-57 -0.5854748626177795 1104 58-59 -0.655241204516436 1104 60-61 -0.6617339455796056 1104 62-63 -0.7135712961213727 1104 64-65 -0.7469984365101041 1104 66-67 -0.7982532454783993 1104 68-69 -0.7889602732274241 1104 70-71 -0.7480955735826385 1104 72-73 -0.6099088863218753 1104 74-75 -0.7277117868278147 1104 76-77 -0.827153847097776 1104 78-79 -0.7830671943316041 1104 80-81 -0.801823025571661 1104 82-83 -0.7708714259833549 1104 84-85 -0.76347928090021 1104 86-87 -0.9386536400601102 1104 88-89 -0.8774142418364193 1104 90-91 -0.9353216096017469 1104 92-93 -1.0450613453489979 1104 94-95 -1.1734780462220407 1104 96-97 -1.0827992270347053 1104 98-99 -1.1023120643620352 1104 100 -1.0470113844310447 1105 1 -0.5271752720887335 1105 2 -0.4827184756278271 1105 3 -0.574084395254232 1105 4 -0.3740695847837614 1105 5 -0.4327507946711435 1105 6 -0.43434241467427626 1105 7 -0.46797787758894316 1105 8 -0.4688808587515112 1105 9 -0.5534361072477836 1105 10-11 -0.5828857386617656 1105 12-13 -0.5850711503944837 1105 14-15 -0.8762545092834273 1105 16-17 -0.8140109617164271 1105 18-19 -0.8055033513190679 1105 20-21 -0.8486432325083229 1105 22-23 -0.721894695779838 1105 24-25 -0.7600870185801725 1105 26-27 -0.7868780968994784 1105 28-29 -0.8407166618865247 1105 30-31 -0.8453617714641624 1105 32-33 -0.9439076037648348 1105 34-35 -0.8800835688226627 1105 36-37 -0.8019467338914268 1105 38-39 -0.9176716782003496 1105 40-41 -0.8651743062387851 1105 42-43 -0.8132152814413871 1105 44-45 -0.8857193971060582 1105 46-47 -0.917433000351366 1105 48-49 -0.9408359383156224 1105 50-51 -0.9748409797476114 1105 52-53 -0.8632424926536189 1105 54-55 -0.891097064392433 1105 56-57 -0.8609307581029739 1105 58-59 -0.7647445666351445 1105 60-61 -0.7409859351207402 1105 62-63 -0.8622648488132398 1105 64-65 -0.7301504036916029 1105 66-67 -0.6628168025128289 1105 68-69 -0.6883986813477208 1105 70-71 -0.6483768748315626 1105 72-73 -0.7220762887384495 1105 74-75 -0.7223658269153859 1105 76-77 -0.8683170650466003 1105 78-79 -0.8566992292373357 1105 80-81 -0.9202407805712376 1105 82-83 -0.9635073854709688 1105 84-85 -0.8743282689187204 1105 86-87 -0.8775517995829354 1105 88-89 -0.9199647709193464 1105 90-91 -0.9639044781557757 1105 92-93 -0.8777943339089553 1105 94-95 -0.9597450016910862 1105 96-97 -0.94485595349569 1105 98-99 -0.9357537793045161 1105 100 -1.0328102534188623 1106 1 -0.525967748159573 1106 2 -0.5431871427127106 1106 3 -0.574707459031238 1106 4 -0.4470130100585905 1106 5 -0.37983489180931684 1106 6 -0.42771531068132873 1106 7 -0.5115342775484422 1106 8 -0.4978412090765474 1106 9 -0.6877451774079333 1106 10-11 -0.805423774997756 1106 12-13 -0.722188459369498 1106 14-15 -0.830213326910993 1106 16-17 -0.7983814224516479 1106 18-19 -0.8392159223382833 1106 20-21 -0.9004201694436134 1106 22-23 -0.8585087902876154 1106 24-25 -0.8579259660380316 1106 26-27 -0.8874206018435444 1106 28-29 -0.9985307923649955 1106 30-31 -0.9660126629217061 1106 32-33 -0.9749833945731297 1106 34-35 -0.9166629518667762 1106 36-37 -0.9704306646079033 1106 38-39 -1.071913860425738 1106 40-41 -1.0369954471864062 1106 42-43 -0.9169652579103484 1106 44-45 -0.9660205333627303 1106 46-47 -0.9844686718814444 1106 48-49 -0.9783089920951085 1106 50-51 -0.9291560382394195 1106 52-53 -0.8768898915960648 1106 54-55 -0.9703470309402036 1106 56-57 -1.005701124408759 1106 58-59 -0.992307624734174 1106 60-61 -0.9370878657344477 1106 62-63 -1.0302229879836915 1106 64-65 -1.0107188462013283 1106 66-67 -0.9582459870567206 1106 68-69 -0.9066368653314498 1106 70-71 -0.8963418214682477 1106 72-73 -0.8369689757491656 1106 74-75 -0.8038835815301439 1106 76-77 -0.8672615707141489 1106 78-79 -0.806912000715581 1106 80-81 -0.9104734275104995 1106 82-83 -0.8634098079724417 1106 84-85 -0.8012448657554394 1106 86-87 -0.9100197693858725 1106 88-89 -0.9955225901185365 1106 90-91 -0.9846177395884261 1106 92-93 -0.9970364474491511 1106 94-95 -0.9197496430297072 1106 96-97 -1.0177612839249086 1106 98-99 -1.1805997820040979 1106 100 -1.1079785703109941 1107 1 -0.49792220822888567 1107 2 -0.4184169168794476 1107 3 -0.4327818810208086 1107 4 -0.305566617764768 1107 5 -0.39859312440454886 1107 6 -0.39541735417762425 1107 7 -0.44188014389607844 1107 8 -0.46692089052798025 1107 9 -0.6061182823165439 1107 10-11 -0.6179040536941827 1107 12-13 -0.6713716952879878 1107 14-15 -0.7900531160759954 1107 16-17 -0.8337643782772588 1107 18-19 -0.7624531399124379 1107 20-21 -0.7732458747445605 1107 22-23 -0.7932469685829702 1107 24-25 -0.8780141697780621 1107 26-27 -0.8391091146793386 1107 28-29 -0.8340363223863605 1107 30-31 -0.883612273128044 1107 32-33 -0.8731259666742162 1107 34-35 -0.9106727947274393 1107 36-37 -0.8689916640264954 1107 38-39 -0.8693742264799909 1107 40-41 -0.7186973000154637 1107 42-43 -0.8183200527215106 1107 44-45 -0.9058071136114876 1107 46-47 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1.275398870751978 2310 32-33 1.2641648385513378 2310 34-35 1.260375799049804 2310 36-37 1.2786452488789308 2310 38-39 1.2493430443325906 2310 40-41 1.158366190904907 2310 42-43 1.2415344865745297 2310 44-45 1.3492106564165525 2310 46-47 1.3619097800919207 2310 48-49 1.4096381441404375 2310 50-51 1.304544694449163 2310 52-53 1.2797379070823638 2310 54-55 1.247325699442385 2310 56-57 1.2531281554457578 2310 58-59 1.2370711539712076 2310 60-61 1.221301333632134 2310 62-63 1.1501685027001471 2310 64-65 1.1251960470429978 2310 66-67 1.085752128231853 2310 68-69 1.1558131458887644 2310 70-71 1.1405491202435982 2310 72-73 1.0889275911502807 2310 74-75 1.0881109296967608 2310 76-77 1.112448944794707 2310 78-79 1.1128980835151303 2310 80-81 1.171637061743457 2310 82-83 1.1486550723743854 2310 84-85 1.2122178151730019 2310 86-87 1.2436571103782406 2310 88-89 1.3331335332694287 2310 90-91 1.3493605276268212 2310 92-93 1.381196709823584 2310 94-95 1.4811467852233662 2310 96-97 1.4931837479968948 2310 98-99 1.4847347466638432 2310 100 1.5167510861747004 2311 1 0.551146685181692 2311 2 0.6227899927310361 2311 3 0.6899841693600344 2311 4 0.5188945847459436 2311 5 0.4805259223609539 2311 6 0.48383548563109713 2311 7 0.570033400528601 2311 8 0.543226424002043 2311 9 0.7279725826677819 2311 10-11 0.675381129192921 2311 12-13 0.7611099200451861 2311 14-15 0.9650072663315399 2311 16-17 0.9883225789124666 2311 18-19 1.0001140059339093 2311 20-21 1.044847355051175 2311 22-23 1.0417562733124228 2311 24-25 1.0152769665793855 2311 26-27 1.0477059633215617 2311 28-29 1.10656622069898 2311 30-31 1.153561050823491 2311 32-33 1.1673796665823062 2311 34-35 1.1672780275185843 2311 36-37 1.1952233532200367 2311 38-39 1.2315477258485785 2311 40-41 1.3057977264277838 2311 42-43 1.3371236428802078 2311 44-45 1.3753077035283638 2311 46-47 1.3603898406912833 2311 48-49 1.3958440488524175 2311 50-51 1.3582806067398607 2311 52-53 1.241467612049874 2311 54-55 1.228667833371432 2311 56-57 1.2662010557597156 2311 58-59 1.1975107303893608 2311 60-61 1.2173699823299344 2311 62-63 1.218666771943532 2311 64-65 1.179119804961303 2311 66-67 1.2001479907078973 2311 68-69 1.215161401119758 2311 70-71 1.2576739034221376 2311 72-73 1.214815516768411 2311 74-75 1.1868596220340564 2311 76-77 1.2233039457012111 2311 78-79 1.1730942643512314 2311 80-81 1.2272998579882746 2311 82-83 1.2535661629860648 2311 84-85 1.2077961183567645 2311 86-87 1.2680280848020438 2311 88-89 1.27302373345427 2311 90-91 1.312207008520371 2311 92-93 1.364365389956614 2311 94-95 1.4115983671439523 2311 96-97 1.5146242844696722 2311 98-99 1.5540829508468832 2311 100 1.5918825353477821 2312 1 0.6149446105007641 2312 2 0.6299177934646494 2312 3 0.728009469809745 2312 4 0.5520944744684684 2312 5 0.5447728071020776 2312 6 0.46151376703201663 2312 7 0.6222413270915297 2312 8 0.6817682124577757 2312 9 0.7896220778485272 2312 10-11 0.8273308672216828 2312 12-13 0.9077463650301212 2312 14-15 1.0902307347508184 2312 16-17 1.096356367177819 2312 18-19 1.0635419878774712 2312 20-21 1.0635510641013326 2312 22-23 1.0572393800320796 2312 24-25 1.0892714794388638 2312 26-27 1.1736051099212048 2312 28-29 1.1712605293221614 2312 30-31 1.1129876604407656 2312 32-33 1.2028406084925436 2312 34-35 1.2111912038397321 2312 36-37 1.2499427061621589 2312 38-39 1.2201630159358956 2312 40-41 1.2970329250737578 2312 42-43 1.3815367964320657 2312 44-45 1.3232362303248024 2312 46-47 1.3107906003925862 2312 48-49 1.387934610914975 2312 50-51 1.4052200009112923 2312 52-53 1.2224628830467026 2312 54-55 1.1994411905648477 2312 56-57 1.1775103970406562 2312 58-59 1.178259719713175 2312 60-61 1.104143924570856 2312 62-63 1.1194298960820248 2312 64-65 1.12962401405904 2312 66-67 1.076957414880944 2312 68-69 1.145394940110826 2312 70-71 1.137844644915866 2312 72-73 1.1160105199832984 2312 74-75 1.0995616845524196 2312 76-77 1.1416431716748718 2312 78-79 1.0800965715575508 2312 80-81 0.9763477690816167 2312 82-83 1.0940379263268518 2312 84-85 1.174794854451779 2312 86-87 1.1778809156735015 2312 88-89 1.238370641159495 2312 90-91 1.3328644047104952 2312 92-93 1.303462376907877 2312 94-95 1.3313639541803468 2312 96-97 1.3289307846904777 2312 98-99 1.2799247061322454 2312 100 1.3042140280985564 2313 1 0.5879020371393224 2313 2 0.6337213285537757 2313 3 0.7009626351016358 2313 4 0.6185666951676012 2313 5 0.5645533310270991 2313 6 0.543652329789289 2313 7 0.5699310986809181 2313 8 0.6436058572535472 2313 9 0.8064558670695376 2313 10-11 0.7124451634167244 2313 12-13 0.8130405448046361 2313 14-15 1.0747563892849001 2313 16-17 1.0405894719024857 2313 18-19 1.0623679531581232 2313 20-21 1.0468698270453416 2313 22-23 1.05245055686472 2313 24-25 1.1504108810071685 2313 26-27 1.1110143489684816 2313 28-29 1.0711553805932112 2313 30-31 1.1466961666901554 2313 32-33 1.1725541389235943 2313 34-35 1.1436637874006266 2313 36-37 1.1382922814744632 2313 38-39 1.1857731662047257 2313 40-41 1.1852621173427806 2313 42-43 1.2083548858270845 2313 44-45 1.1951465753129114 2313 46-47 1.2025789543533065 2313 48-49 1.1950651708362372 2313 50-51 1.159435551584771 2313 52-53 1.0084738447946222 2313 54-55 0.980624660121471 2313 56-57 1.021637267471796 2313 58-59 1.0023197401336645 2313 60-61 0.9935906752818866 2313 62-63 1.0107223695378167 2313 64-65 1.0269528199183142 2313 66-67 0.9519393951832242 2313 68-69 1.0049874917191701 2313 70-71 1.0000872956504239 2313 72-73 0.9666664882245044 2313 74-75 1.0144371516856907 2313 76-77 1.0429504638288307 2313 78-79 1.0491821332223275 2313 80-81 1.1022968188566047 2313 82-83 1.1274750212039244 2313 84-85 1.1230331463141603 2313 86-87 1.1615076418256933 2313 88-89 1.2101638301304511 2313 90-91 1.2773881912643859 2313 92-93 1.3182245448124341 2313 94-95 1.3596368835090686 2313 96-97 1.424527496074404 2313 98-99 1.3943979021403585 2313 100 1.505906154918069 2314 1 0.5833310756586805 2314 2 0.5406144116563993 2314 3 0.599515389236263 2314 4 0.43329210003325613 2314 5 0.4442086768719662 2314 6 0.48468432458876265 2314 7 0.5760772962584824 2314 8 0.569767446759819 2314 9 0.6851967999454658 2314 10-11 0.6598898182154826 2314 12-13 0.4450213223636794 2314 14-15 0.8093386234684061 2314 16-17 0.8134461642193074 2314 18-19 0.8397653104172917 2314 20-21 0.8797096654760566 2314 22-23 0.680507266099788 2314 24-25 0.7680652577305978 2314 26-27 0.9319757376624906 2314 28-29 0.977873074782984 2314 30-31 0.8813552225966887 2314 32-33 0.9142544965054924 2314 34-35 1.017620625637342 2314 36-37 1.0917328004969136 2314 38-39 1.162414856999824 2314 40-41 1.1010085017566595 2314 42-43 1.1893544049442468 2314 44-45 1.221713421245589 2314 46-47 1.2021976960953182 2314 48-49 1.224758536767851 2314 50-51 1.2024178802897936 2314 52-53 1.010303590202554 2314 54-55 1.0266506186000441 2314 56-57 1.0002737485495672 2314 58-59 1.0330819440298242 2314 60-61 1.0087985260849948 2314 62-63 0.9879191903781575 2314 64-65 1.0210019637537826 2314 66-67 1.0595250691041969 2314 68-69 1.0469141308814258 2314 70-71 1.0609563051880784 2314 72-73 1.0664618141904327 2314 74-75 1.0792621351582241 2314 76-77 1.0793139413749202 2314 78-79 1.0851382855343807 2314 80-81 1.1711159342712456 2314 82-83 1.0633179407788056 2314 84-85 1.0252214390697496 2314 86-87 1.3090431862393146 2314 88-89 1.3850151288359278 2314 90-91 1.4367648549305123 2314 92-93 1.4539929413394113 2314 94-95 1.5024844638776464 2314 96-97 1.5761537627593398 2314 98-99 1.6261676613219542 2314 100 1.591555342152514 2315 1 0.3354022584135805 2315 2 0.3880148763618365 2315 3 0.45888306446468974 2315 4 0.4090314238440058 2315 5 0.32642262701099156 2315 6 -0.3406008633843811 2315 7 0.1926528527116247 2315 8 0.21793188067801594 2315 9 0.46227350337240836 2315 10-11 0.5440211500310781 2315 12-13 0.5907667393133949 2315 14-15 0.7188911583366107 2315 16-17 0.6639738983927046 2315 18-19 0.697754194614248 2315 20-21 0.7303342472819665 2315 22-23 0.6197501919896737 2315 24-25 0.7281443729134907 2315 26-27 0.7763879441871424 2315 28-29 0.8601008521017732 2315 30-31 0.8851982159586314 2315 32-33 0.969459693383115 2315 34-35 0.9529967478764974 2315 36-37 0.9777784221577406 2315 38-39 1.018878607731665 2315 40-41 1.0306446741689044 2315 42-43 1.0111951270532487 2315 44-45 1.0548426427109874 2315 46-47 1.0272971191914593 2315 48-49 1.0589527698202872 2315 50-51 0.9778344105333829 2315 52-53 0.8626155405578118 2315 54-55 0.6930886746196947 2315 56-57 0.6621222391238817 2315 58-59 0.6253289697518198 2315 60-61 0.5956352804500185 2315 62-63 0.7260416620888606 2315 64-65 0.8182289607835784 2315 66-67 0.858266311252855 2315 68-69 0.8720296060009147 2315 70-71 0.8694002240849485 2315 72-73 0.851023235683158 2315 74-75 0.905278372308949 2315 76-77 0.8853481187973316 2315 78-79 0.854819761881938 2315 80-81 0.8357582482717696 2315 82-83 1.0076476559682312 2315 84-85 0.9990978994341297 2315 86-87 1.0387582672873705 2315 88-89 1.2660643382960934 2315 90-91 1.2801827093704112 2315 92-93 1.3594234594104968 2315 94-95 1.3658415067761993 2315 96-97 1.3950372597493583 2315 98-99 1.5426186839085183 2315 100 1.5111226438622118 2316 1 -0.018327127303116697 2316 2 -0.059545266213049786 2316 3 0.0522316630409243 2316 4 -0.0010676513056679937 2316 5 -0.017937672435984098 2316 6 0.09728329948459447 2316 7 0.1173777060163701 2316 8 0.08987352028920981 2316 9 0.22943157434213646 2316 10-11 0.15691934947705732 2316 12-13 0.20083258463552056 2316 14-15 0.3946520748469027 2316 16-17 0.4337071227595004 2316 18-19 0.37150964294077937 2316 20-21 0.37952819901897783 2316 22-23 0.2725531388020883 2316 24-25 0.34318272218224166 2316 26-27 0.3706580483879378 2316 28-29 0.53750083993922 2316 30-31 0.4869007807676482 2316 32-33 0.5272702293793046 2316 34-35 0.5679010236338016 2316 36-37 0.6102367033658496 2316 38-39 0.6628929446492293 2316 40-41 0.6809903078129977 2316 42-43 0.7395506756587764 2316 44-45 0.5279731468859623 2316 46-47 0.4892244367780165 2316 48-49 0.5533606459409768 2316 50-51 0.34300948301225986 2316 52-53 0.15006483016553318 2316 54-55 0.11961240028218612 2316 56-57 0.23974142592080483 2316 58-59 0.2585070816274353 2316 60-61 0.3808066234935339 2316 62-63 0.44544803030140656 2316 64-65 0.4273318795476726 2316 66-67 0.42248834855465844 2316 68-69 0.38347336288888556 2316 70-71 0.21735128719121377 2316 72-73 0.3313793502196418 2316 74-75 0.4669071861375258 2316 76-77 0.6101142068740586 2316 78-79 0.6464349114564918 2316 80-81 0.7024069317376451 2316 82-83 0.7669865902805206 2316 84-85 0.7740915699431703 2316 86-87 0.9578556136172587 2316 88-89 0.9958140069286223 2316 90-91 0.9698135387074771 2316 92-93 1.073732266586898 2316 94-95 1.1369271758158064 2316 96-97 1.239753357258273 2316 98-99 1.3534341395325775 2316 100 1.1545038331115123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 32.0 1 399.0 2 185.0 3 142.0 4 123.0 5 106.0 6 127.0 7 684.0 8 2330.0 9 4511.0 10 6477.0 11 8788.0 12 11369.0 13 13688.0 14 16187.0 15 20159.0 16 26142.0 17 33637.0 18 42010.0 19 50680.0 20 60468.0 21 71215.0 22 78236.0 23 80931.0 24 81128.0 25 84513.0 26 92732.0 27 107070.0 28 126995.0 29 149424.0 30 181410.0 31 223282.0 32 280292.0 33 361136.0 34 497433.0 35 660241.0 36 939779.0 37 1368277.0 38 1283917.0 39 335783.0 40 458.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.97108423351151 18.144246167595288 12.235725981330422 23.648943617562782 2 16.495886492142574 20.43698221159846 40.30834509306992 22.75878620318905 3 18.819700342715535 26.978223092323905 30.357013957498125 23.845062607462435 4 12.736471105174546 16.792756282383028 37.76743992377918 32.70333268866325 5 12.74402788064295 37.511507696202024 34.04664498519221 15.697819437962815 6 29.37297220273588 36.38828755261312 19.786218103297088 14.45252214135391 7 26.758181310201728 33.20830022525143 22.768385108001887 17.265133356544958 8 26.205708701873725 34.64879349662648 21.069170412127676 18.076327389372114 9 28.08112012461051 15.697115368149658 20.490441527519863 35.73132297971997 10-11 25.021861412821973 25.574962349598756 29.149137236243405 20.254039001335865 12-13 25.3555726085709 23.985162735016576 28.426800426360394 22.232464230052134 14-15 23.207836157307995 25.426790955108714 26.70298803832748 24.662384849255805 16-17 22.146124443973285 27.63779265492082 27.450787432580668 22.765295468525228 18-19 21.667641507553938 27.10919854656599 29.454048698763756 21.76911124711631 20-21 22.776700292427698 26.762571994055566 28.840863175340196 21.61986453817654 22-23 22.513517171060254 26.534028041640862 28.796929998901476 22.15552478839741 24-25 21.995683009221167 26.183238027979765 29.243263758987055 22.577815203812012 26-27 21.939239550345363 26.364447510034257 29.05040045598518 22.645912483635204 28-29 21.909606475196007 27.475398236730676 28.769604693285793 21.845390594787524 30-31 23.238508603460147 26.47940719963048 28.97568952830515 21.306394668604227 32-33 22.882316949477328 26.4754366805498 28.725747369808087 21.91649900016478 34-35 22.758853639923153 26.833522003606387 28.833130120987082 21.574494235483375 36-37 22.454721943274457 26.766690187082848 28.777997382323555 22.000590487319144 38-39 22.359907169668613 26.922549323561316 28.742399939513863 21.975143567256207 40-41 22.40555388487793 26.123986895343204 29.302736624737946 22.167722595040914 42-43 22.10475820695859 26.67154954125795 28.528592041101625 22.695100210681836 44-45 21.310401476453137 26.985340131096592 29.243288998824053 22.460969393626222 46-47 21.308600888962932 26.250463743606918 28.86973147532665 23.571203892103508 48-49 21.677450183004005 26.475118318661917 28.850389345011834 22.997042153322244 50-51 21.640533610210863 26.821631787585986 28.810485914586202 22.727348687616946 52-53 21.400178852337906 27.09247131666762 28.354705765299286 23.15264406569518 54-55 21.110615641009307 26.57381054638936 29.07654191094827 23.239031901653064 56-57 21.675814348747735 26.569578804759196 28.463014051075312 23.291592795417753 58-59 21.34958412730098 26.782634750269164 28.677642024162463 23.190139098267398 60-61 21.946418633600096 26.653841038458122 28.490536653304105 22.909203674637677 62-63 21.86576493762671 26.451992366149767 28.462966471026313 23.219276225197213 64-65 21.59104770022811 26.20983645943291 28.34371455130873 23.855401289030244 66-67 21.577280580104336 26.20693887458487 28.058421381101127 24.15735916420966 68-69 21.27521609885067 26.456780229866162 28.25996246523925 24.008041206043917 70-71 21.546633941646363 26.27386367785089 28.337842818664306 23.841659561838448 72-73 21.748913327005827 26.835589609148435 27.702859325440755 23.712637738404986 74-75 22.149391768152785 26.82842807577935 27.467266702919048 23.55491345314882 76-77 22.04369778560851 26.555241723630314 27.526054806723543 23.875005684037635 78-79 21.87353962602373 26.75866006904059 27.874652865852955 23.49314743908272 80-81 22.213913581584464 26.786513348163332 27.40916374502172 23.59040932523048 82-83 22.752853002569484 26.713951908484773 27.497274375845766 23.03592071309998 84-85 22.87008105788931 27.088575453698134 26.896680037419024 23.144663450993537 86-87 22.487949451724877 27.207224234356204 27.246092773642804 23.05873354027612 88-89 22.62339379909239 26.521228073851855 27.302583686393817 23.55279444066194 90-91 23.56018766008777 26.31257076870756 27.11367219585395 23.01356937535072 92-93 23.62423902499323 26.423038243327785 26.94704993886176 23.005672792817226 94-95 23.507143870729855 26.248540571695937 26.892894262056615 23.35142129551759 96-97 23.611768579790947 26.06927692690373 26.87294335578589 23.446011137519438 98-99 23.67827913132223 26.201743900420247 26.55770582256942 23.5622711456881 100 24.076172650592852 26.144576646394157 26.475834146877354 23.303416556135634 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3200.0 1 2286.0 2 3404.0 3 5129.0 4 3512.0 5 2383.5 6 3163.0 7 5335.0 8 8290.5 9 9582.5 10 8247.5 11 6859.5 12 6939.0 13 7573.0 14 8334.5 15 9041.0 16 9772.5 17 10502.0 18 11387.0 19 12419.5 20 13447.5 21 15011.0 22 17251.5 23 20145.5 24 23814.0 25 28698.5 26 35110.0 27 42468.5 28 51303.0 29 62829.0 30 75986.0 31 88160.5 32 101522.5 33 114854.5 34 127748.0 35 141491.5 36 157047.0 37 175666.0 38 187721.0 39 192085.0 40 196403.5 41 201426.5 42 208547.5 43 219756.5 44 232948.0 45 250096.0 46 268342.0 47 284589.0 48 302577.5 49 320504.0 50 334492.5 51 338602.5 52 326670.0 53 306262.5 54 284200.5 55 255794.5 56 226829.5 57 197821.0 58 167701.5 59 138286.0 60 107283.0 61 81341.0 62 62702.5 63 48096.0 64 35427.5 65 25070.5 66 18167.0 67 13827.0 68 10498.5 69 7725.5 70 5769.5 71 4263.5 72 3208.5 73 2429.0 74 1816.5 75 1320.0 76 944.0 77 702.0 78 513.5 79 339.0 80 234.0 81 172.0 82 131.5 83 111.5 84 79.0 85 54.5 86 53.0 87 49.5 88 35.5 89 34.0 90 31.0 91 23.5 92 18.5 93 15.0 94 11.5 95 8.0 96 6.5 97 6.0 98 3.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21750097500909277 2 0.14605964864616153 3 0.023129078057694246 4 0.021965092483446757 5 0.02666211662423369 6 0.05389937906162495 7 0.0606504953922604 8 0.05318729376914414 9 0.040410840348286396 10-11 0.0425197083298642 12-13 0.04159536684443237 14-15 0.020109562538616933 16-17 0.018664851031756812 18-19 0.01786375507771589 20-21 0.021170843503372 22-23 0.024402615215400325 24-25 0.021595355889274023 26-27 0.018918189068504794 28-29 0.018384125099144184 30-31 0.01668607555553608 32-33 0.024553248642655883 34-35 0.025203711169441242 36-37 0.023916480063802842 38-39 0.022588167114367473 40-41 0.023724764792750316 42-43 0.02946252897639382 44-45 0.03889081212779849 46-47 0.043875409175164214 48-49 0.04010957349377528 50-51 0.02788087799021047 52-53 0.04352621350288997 54-55 0.04150635618287227 56-57 0.04377955153963795 58-59 0.04720988549668497 60-61 0.02395071493363365 62-63 0.025402273414459935 64-65 0.02590210251398974 66-67 0.03138652866088526 68-69 0.035549488832311585 70-71 0.03947280491492224 72-73 0.0431633238826834 74-75 0.025005148924422554 76-77 0.01845944181277196 78-79 0.017583029145103263 80-81 0.0209380463885225 82-83 0.019390630272169956 84-85 0.011811030091628946 86-87 0.0131598839629628 88-89 0.01833619628138105 90-91 0.019486487907696217 92-93 0.01942486514200076 94-95 0.023170159901491218 96-97 0.024210899944347795 98-99 0.029257119757408974 100 0.029209190939645843 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7302496.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.784252204610304 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74079726288223 48.80897821940276 2 14.108945606110069 17.71639198550286 3 3.9618529491212335 7.4622592426561996 4 1.553225533852152 3.9007241459205595 5 0.7682856132869332 2.4118118854890263 6 0.44243037137836216 1.6666596011759096 7 0.27946611368963536 1.2282249675172654 8 0.1957712745271396 0.983308245946395 9 0.1510371593200469 0.8534479592715961 >10 0.7382163435796171 8.138038974874508 >50 0.03981813864113696 1.6782157640146935 >100 0.017313921433501474 2.0020322809374456 >500 0.001841996634816788 0.7787587916846535 >1k 8.442208445833422E-4 0.9748357807843842 >5k 7.67473492618632E-5 0.30761463166476166 >10k+ 7.67473492618632E-5 1.0886975231568963 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29598 0.4053134709009084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23528 0.3221912069517053 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14656 0.20069850089613198 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7848 0.10747010337287416 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3693947932323413E-5 2 0.0 0.0 0.0 0.0 1.3693947932323413E-5 3 0.0 0.0 0.0 0.0 1.3693947932323413E-5 4 0.0 0.0 0.0 0.0 1.3693947932323413E-5 5 0.0 0.0 0.0 1.3693947932323413E-5 1.3693947932323413E-5 6 0.0 0.0 0.0 1.3693947932323413E-5 2.7387895864646827E-5 7 0.0 0.0 0.0 1.3693947932323413E-5 2.7387895864646827E-5 8 0.0 0.0 0.0 1.3693947932323413E-5 2.7387895864646827E-5 9 0.0 0.0 0.0 2.7387895864646827E-5 4.108184379697024E-5 10-11 0.0 0.0 0.0 4.108184379697024E-5 4.108184379697024E-5 12-13 0.0 0.0 0.0 6.846973966161707E-5 4.108184379697024E-5 14-15 0.0 0.0 0.0 1.0955158345858732E-4 6.162276569545536E-5 16-17 0.0 0.0 0.0 1.5748040122171925E-4 6.846973966161707E-5 18-19 0.0 0.0 0.0 2.6018501071414487E-4 8.216368759394048E-5 20-21 0.0 0.0 0.0 5.751458131575834E-4 8.216368759394048E-5 22-23 0.0 0.0 0.0 0.0021362558774424526 8.216368759394048E-5 24-25 0.0 0.0 0.0 0.006600482903379886 1.0955158345858731E-4 26-27 0.0 0.0 0.0 0.013844581359578972 1.0955158345858731E-4 28-29 0.0 0.0 0.0 0.03615202254133381 1.0955158345858731E-4 30-31 0.0 0.0 0.0 0.10799047339430243 1.1639855742474902E-4 32-33 0.0 0.0 0.0 0.2689422904168657 1.2324553139091073E-4 34-35 0.0 0.0 0.0 0.5035743942892951 1.2324553139091073E-4 36-37 0.0 0.0 0.0 0.8537012550229401 1.2324553139091073E-4 38-39 0.0 0.0 0.0 1.3827258515444583 1.5748040122171925E-4 40-41 0.0 0.0 0.0 1.9864920158805974 2.054092189848512E-4 42-43 0.0 0.0 0.0 2.5372009789529497 2.054092189848512E-4 44-45 0.0 0.0 0.0 3.1543255894970708 2.2595014088333635E-4 46-47 0.0 0.0 0.0 3.851628265184945 2.6018501071414487E-4 48-49 0.0 0.0 0.0 4.573675904786528 2.875729065787917E-4 50-51 0.0 0.0 0.0 5.309581819695621 2.875729065787917E-4 52-53 0.0 0.0 0.0 6.039078966972388 2.944198805449534E-4 54-55 0.0 0.0 0.0 6.845536101628813 3.012668545111151E-4 56-57 0.0 0.0 0.0 7.667159283620285 3.012668545111151E-4 58-59 0.0 0.0 0.0 8.561709585325346 3.081138284772768E-4 60-61 0.0 0.0 0.0 9.449871660319978 3.149608024434385E-4 62-63 0.0 0.0 0.0 10.379697571898705 3.286547503757619E-4 64-65 0.0 0.0 0.0 11.253364603006972 3.4234869830808537E-4 66-67 0.0 0.0 0.0 12.171085064613523 3.560426462404088E-4 68-69 0.0 0.0 0.0 13.173714850374449 3.697365941727322E-4 70-71 0.0 0.0 0.0 14.199247764052181 3.697365941727322E-4 72-73 0.0 0.0 0.0 15.123335911447263 4.313593598681875E-4 74-75 0.0 0.0 0.0 16.039597967599022 4.4505330780051093E-4 76-77 0.0 0.0 0.0 17.015086348558082 4.5190028176667264E-4 78-79 0.0 0.0 0.0 18.054094107001223 4.929821255636429E-4 80-81 0.0 0.0 0.0 19.03952429415915 4.998290995298046E-4 82-83 0.0 0.0 0.0 20.024803847889817 5.203700214282897E-4 84-85 0.0 0.0 0.0 20.98517411033159 5.203700214282897E-4 86-87 0.0 0.0 0.0 21.942449540540657 5.477579172929365E-4 88 0.0 0.0 0.0 22.672015157557087 5.751458131575834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13020 0.0 51.016712 1 TACCTGG 7965 0.0 50.98479 2 ACCTGGG 9505 0.0 42.23349 3 ATAACGC 815 0.0 41.526573 3 GTATCAA 23430 0.0 41.418972 1 TAACGCA 855 0.0 37.384712 4 CAACGCA 28805 0.0 33.0452 5 ATCAACG 28835 0.0 33.02712 3 TATCAAC 29335 0.0 32.739536 2 CCTGGGG 9290 0.0 32.079292 4 TCAACGC 29735 0.0 32.0749 4 AACGCAG 30770 0.0 31.332306 6 GTACCTG 13350 0.0 30.876719 1 GTACATG 25815 0.0 29.982635 1 TACATGG 25820 0.0 29.214127 2 ACGCAGA 34060 0.0 28.002357 7 ACATGGG 26035 0.0 27.804453 3 CATGGGG 20335 0.0 27.368988 4 CGCAGAG 34900 0.0 27.260098 8 ATGGGGG 16265 0.0 27.223757 5 >>END_MODULE