FastQCFastQC Report
Mon 6 Jun 2016
ERR1211395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211395_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7302496
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268830.36813440226465033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226870.3106745967406213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145830.19969884269707233No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG78200.051.7993162
GGTATCA128300.051.558021
ACCTGGG89550.044.1863253
GTATCAA226600.039.3375431
CCTGGGG81900.035.174414
ATCAACG280700.031.2064863
CAACGCA284650.030.8523965
TATCAAC287000.030.8476812
TCAACGC292150.030.0805664
GTACCTG138000.029.6734371
AACGCAG303450.029.3423616
TGGGGGG156050.029.2668156
GTACATG242650.028.830451
TACATGG240950.028.3960532
CTGGGGG61450.028.3689215
ATAACGC8350.027.5772513
ATGGGGG148700.027.0491495
ACATGGG240100.026.9906583
GGGTTAG29500.026.9335441
CATGGGG184800.026.9050624
Produced by FastQC (version 0.11.5)