FastQCFastQC Report
Mon 6 Jun 2016
ERR1211394_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211394_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8772532
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345330.39364917677131306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271190.3091353784745385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171260.19522299833161055No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88320.10067788866429897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141500.055.9017071
TACCTGG92200.051.7097742
GTATCAA271800.043.177261
ACCTGGG109200.043.1767043
ATCAACG329650.034.9654053
CAACGCA333050.034.6362885
TATCAAC337900.034.5288772
GTACATG331150.033.8596951
TCAACGC345250.033.466444
CCTGGGG107250.033.3540534
AACGCAG354950.033.0431946
TACATGG337250.032.6974952
ACATGGG343700.030.6228623
ATGGGGG221000.030.5867165
CATGGGG271200.030.453934
TGGGGGG248000.030.1576236
GTACCTG164150.029.8772541
ACGCAGA394800.029.1725927
CGCAGAG402900.028.4903168
GGGTTAG32450.028.4558891
Produced by FastQC (version 0.11.5)